Restriction map for
pFN21AE9545
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MluI MroI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1270 GTCTAC GT^[AC][GT]AC
AccII 2235 CGCG CG^CG
AcyI 442 GGCGTC G[GA]^CG[CT]C
AhdI 1762 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 531 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1762 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 881 TGGCCA TGG^CCA
BamHI 2719 GGATCC G^GATCC
BbsI 2761 GAAGAC GAAGAC(2/6)
BciVI 280 GGATAC GTATCC(6/5)
BpuAI 2761 GAAGAC GAAGAC(2/6)
BsaHI 442 GGCGTC G[GA]^CG[CT]C
BsiEI 658 CGGCCG CG[GA][CT]^CG
Bsp1407I 1183 TGTACA T^GTACA
BsrFI 2043 GCCGGC [GA]^CCGG[CT]
BsrGI 1183 TGTACA T^GTACA
BssSI 2296 CACGAG CACGAG(-5/-1)
BstUI 2235 CGCG CG^CG
Cfr10I 2043 GCCGGC [GA]^CCGG[CT]
EagI 658 CGGCCG C^GGCCG
Eam1105I 1762 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 658 CGGCCG C^GGCCG
Eco52I 658 CGGCCG C^GGCCG
EcoT22I 2079 ATGCAT ATGCA^T
Hin1I 442 GGCGTC G[GA]^CG[CT]C
MfeI 632 CAATTG C^AATTG
MluNI 881 TGGCCA TGG^CCA
MscI 881 TGGCCA TGG^CCA
MunI 632 CAATTG C^AATTG
MvnI 2235 CGCG CG^CG
NaeI 2043 GCCGGC GCC^GGC
NgoMIV 2043 GCCGGC G^CCGGC
NsiI 2079 ATGCAT ATGCA^T
PvuII 638 CAGCTG CAG^CTG
SacI 1945 GAGCTC GAGCT^C
SexAI 1451 ACCTGGT A^CC[AT]GGT
SfiI 882 GGCCAGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2654 CCCGGG CCC^GGG
SphI 2429 GCATGC GCATG^C
SspBI 1183 TGTACA T^GTACA
XmaCI 2654 CCCGGG C^CCGGG
XmaI 2654 CCCGGG C^CCGGG
XmnI 531 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaI 2654 CCCGGG C^[CT]CG[GA]G
2779 CCCGAG
BbrPI 934 CACGTG CAC^GTG
2289 CACGTG
BbvCI 519 CCTCAGC CCTCAGC(-5/-2)
688 GCTGAGG
BcgI 2054 GCATCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2597 GCAACACCATCG
BsaAI 934 CACGTG [CT]AC^GT[GA]
2289 CACGTG
BsaI 1213 GAGACC GGTCTC(1/5)
3113 GAGACC
BsgI 2190 CTGCAC GTGCAG(16/14)
1346 GTGCAG
BsmBI 444 CGTCTC CGTCTC(1/5)
2679 CGTCTC
BsoBI 2654 CCCGGG C^[CT]CG[GA]G
2779 CCCGAG
BsrDI 1278 CATTGC GCAATG(2/0)
1794 CATTGC
BstAPI 2072 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2169 GCAGTTCCTGC
EcoRV 700 GATATC GAT^ATC
1245 GATATC
HgaI 273 GACGC GACGC(5/10)
443 GCGTC
MseI 1067 TTAA T^TAA
1514 TTAA
NcoI 587 CCATGG C^CATGG
2957 CCATGG
NspI 759 GCATGT [GA]CATG^[CT]
2429 GCATGC
PmaCI 934 CACGTG CAC^GTG
2289 CACGTG
PmlI 934 CACGTG CAC^GTG
2289 CACGTG
SapI 474 GCTCTTC GCTCTTC(1/4)
1326 GCTCTTC
ScaI 734 AGTACT AGT^ACT
926 AGTACT
SmlI 826 CTTGAG C^T[CT][GA]AG
2244 CTCAAG
StuI 317 AGGCCT AGG^CCT
890 AGGCCT
Tru9I 1067 TTAA T^TAA
1514 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbvI BcnI BglI BglII
BmyI BpmI BsaBI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MamI
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII