Restriction map for
pFN21AE9545
(Circular) (Six-base) MAPSORT of: pFN21AE9545.seq Check: 4880 from: 1 to: 7939
pFN21AE9545 7939 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3421 3692 4352 3421
Size: 271 660 7008
Fragments arranged by size:
7008 660 271
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5749 278
Size: 53 83 186 936 4213 2468
Fragments arranged by size:
4213 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3276 5282 1937
Size: 1339 2006 4594
Fragments arranged by size:
4594 2006 1339
Acc65I G'GTAC_C
Cuts at: 5260 5260
Size: 7939
AclI AA'CG_TT
Cuts at: 1563 6066 6439 1563
Size: 4503 373 3063
Fragments arranged by size:
4503 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2704 2990 3026 3407 4414 5166 6002 7386
Size: 1072 286 36 381 1007 752 836 1384
Cuts at: 7386 7654 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1072 1007 836 752 381 286
268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7742
AflIII A'CryG_T
Cuts at: 1203 6838 7815 1203
Size: 5635 977 1327
Fragments arranged by size:
5635 1327 977
AgeI A'CCGG_T
Cuts at: 6770 6770
Size: 7939
AhdI GACnn_n'nnGTC
Cuts at: 3772 6668 3772
Size: 2896 5043
AleI CACnn'nnGTG
Cuts at: 1166 2459 1166
Size: 1293 6646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2073 4220 1184
Size: 889 2147 4903
Fragments arranged by size:
4903 2147 889
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 7907
AlwNI CAG_nnn'CTG
Cuts at: 3385 3747 4180 4270 4810 4846 5682 7254 3385
Size: 362 433 90 540 36 836 1572 4070
Fragments arranged by size:
4070 1572 836 540 433 362 90 36
ApaLI G'TGCA_C
Cuts at: 5996 7152 5996
Size: 1156 6783
ApoI r'AATT_y
Cuts at: 1493 2678 2785 2999 4975 5233 5248 5474 5626
Size: 1185 107 214 1976 258 15 226 152
Cuts at: 5626 7735 1493
Size: 2109 1697
Fragments arranged by size:
2109 1976 1697 1185 258 226 214 152
107 15
AseI AT'TA_AT
Cuts at: 160 1032 6493 160
Size: 872 5461 1606
Fragments arranged by size:
5461 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7939
AvaI C'yCGr_G
Cuts at: 1870 1943 4659 4784 5264 1870
Size: 73 2716 125 480 4545
Fragments arranged by size:
4545 2716 480 125 73
BamHI G'GATC_C
Cuts at: 4724 5269 5636 4724
Size: 545 367 7027
Fragments arranged by size:
7027 545 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2951 3486 3613 5260 6715
Size: 325 217 627 1164 535 127 1647 1455
Cuts at: 6715 618
Size: 1842
Fragments arranged by size:
1842 1647 1455 1164 627 535 325 217
127
BanII G_rGCy'C
Cuts at: 1391 2083 2115 2481 2838 3333 3681 3837 3954
Size: 692 32 366 357 495 348 156 117
Cuts at: 3954 4020 4167 4310 5258 1391
Size: 66 147 143 948 4072
Fragments arranged by size:
4072 948 692 495 366 357 348 156
147 143 117 66 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4773 928
Size: 971 2874 4094
Fragments arranged by size:
4094 2874 971
BbvCI CC'TCA_GC
Cuts at: 2525 2694 2525
Size: 169 7770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 4591 4625 6130 6164 1903
Size: 34 2111 34 509 34 1505 34 3678
Fragments arranged by size:
3678 2111 1505 509 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2279 5831 7041 2279
Size: 3552 1210 3177
Fragments arranged by size:
3552 3177 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7939
BfrBI ATG'CAT
Cuts at: 4086 4086
Size: 7939
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2894 4305 4529
Size: 107 122 71 631 605 1222 1411 224
Cuts at: 4529 4713 4789 6550 136
Size: 184 76 1761 1525
Fragments arranged by size:
1761 1525 1411 1222 631 605 224 184
122 107 76 71
BglII A'GATC_T
Cuts at: 2152 2827 2877 4572 5074 7934 2152
Size: 675 50 1695 502 2860 2157
Fragments arranged by size:
2860 2157 1695 675 502 50
BlpI GC'TnA_GC
Cuts at: 5356 5356
Size: 7939
Bme1580I G_kGCm'C
Cuts at: 1790 2636 3618 3761 6000 7156 1790
Size: 846 982 143 2239 1156 2573
Fragments arranged by size:
2573 2239 1156 982 846 143
BmgBI CAC'GTC
Cuts at: 1318 4032 1318
Size: 2714 5225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2635 2731 5670
Size: 416 53 83 399 24 1218 96 2939
Cuts at: 5670 6628 442
Size: 958 1753
Fragments arranged by size:
2939 1753 1218 958 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 3581 3523
Size: 58 7881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2497 2800 3589 3976 4292 5018 6599 1239
Size: 1258 303 789 387 316 726 1581 2579
Fragments arranged by size:
2579 1581 1258 789 726 387 316 303
Bpu10I CC'TnA_GC
Cuts at: 2443 2525 2694 2443
Size: 82 169 7688
Fragments arranged by size:
7688 169 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2852 4234 6064 6929 7227 2852
Size: 1382 1830 865 298 3564
Fragments arranged by size:
3564 1830 1382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3212 5112 6602 882
Size: 617 1713 1900 1490 2219
Fragments arranged by size:
2219 1900 1713 1490 617
BsaAI yAC'GTr
Cuts at: 493 1204 2941 4296 5730 493
Size: 711 1737 1355 1434 2702
Fragments arranged by size:
2702 1737 1434 1355 711
BsaBI GATnn'nnATC
Cuts at: 2151 4214 4523 4577 7933 2151
Size: 2063 309 54 3356 2157
Fragments arranged by size:
3356 2157 2063 309 54
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2448 5746 6128 6763
Size: 53 83 186 936 915 3298 382 635
Cuts at: 6763 275
Size: 1451
Fragments arranged by size:
3298 1451 936 915 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6372 6770 7044 7191 7602 6372
Size: 398 274 147 411 6709
Fragments arranged by size:
6709 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3776 3800 3806 3830 4292
Size: 30 165 30 2373 24 6 24 462
Cuts at: 4292 4322 4976 5006 1178
Size: 30 654 30 4111
Fragments arranged by size:
4111 2373 654 462 165 30 30 30
30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2500 2513 2899 3593 3953 3956 4628
Size: 1069 245 13 386 694 360 3 672
Cuts at: 4628 4726 4729 1186
Size: 98 3 4396
Fragments arranged by size:
4396 1069 694 672 386 360 245 98
13 3 3
BseYI C'CCAG_C
Cuts at: 2025 2257 2281 2473 2975 3868 3895 4821 5158
Size: 232 24 192 502 893 27 926 337
Cuts at: 5158 7142 7638 2025
Size: 1984 496 2326
Fragments arranged by size:
2326 1984 926 893 502 496 337 232
192 27 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3372 4180 1163
Size: 573 1636 808 4922
Fragments arranged by size:
4922 1636 808 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2666 5389 6150 7178 1540
Size: 275 186 665 2723 761 1028 2301
Fragments arranged by size:
2723 2301 1028 761 665 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2580 3954 4074 6000 6085 7156 2406
Size: 174 1374 120 1926 85 1071 3189
Fragments arranged by size:
3189 1926 1374 1071 174 120 85
BsmI GAATG_Cn'
Cuts at: 5450 5543 5450
Size: 93 7846
BsmBI CGTCTCn'nnnn_
Cuts at: 2455 4690 2455
Size: 2235 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2115 2406 2481 2580 2636 2838
Size: 399 293 32 291 75 99 56 202
Cuts at: 2838 3333 3618 3681 3761 3837 3954 4020 4074
Size: 495 285 63 80 76 117 66 54
Cuts at: 4074 4167 4310 5258 6000 6085 7156 1391
Size: 93 143 948 742 85 1071 2174
Fragments arranged by size:
2174 1071 948 742 495 399 293 291
285 202 143 117 99 93 85 80
76 75 66 63 56 54 32
BspEI T'CCGG_A
Cuts at: 7602 7602
Size: 7939
BspHI T'CATG_A
Cuts at: 5828 7558 5828
Size: 1730 6209
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 3370 3421 3692 4179 4352 5294 844
Size: 2476 50 51 271 487 173 942 3489
Fragments arranged by size:
3489 2476 942 487 271 173 51 50
BsrBI CCG'CTC
Cuts at: 1394 1823 5826 1394
Size: 429 4003 3507
Fragments arranged by size:
4003 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3282 3798 6434 6608 65
Size: 1183 2034 516 2636 174 1396
Fragments arranged by size:
2636 2034 1396 1183 516 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 6583 6770 1700
Size: 110 2238 2535 187 2869
Fragments arranged by size:
2869 2535 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 3188 96
Size: 3092 4847
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7939
BssSI C'ACGA_G
Cuts at: 4301 5999 7011 4301
Size: 1698 1012 5229
Fragments arranged by size:
5229 1698 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4083 4180 794
Size: 3289 97 4553
Fragments arranged by size:
4553 3289 97
BstBI TT'CG_AA
Cuts at: 6743 6743
Size: 7939
BstEII G'GTnAC_C
Cuts at: 1184 3433 3452 4921 1184
Size: 2249 19 1469 4202
Fragments arranged by size:
4202 2249 1469 19
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2593 2945 2461
Size: 132 352 7455
Fragments arranged by size:
7455 352 132
BstYI r'GATC_y
Cuts at: 1972 2152 2757 2827 2877 2982 4131 4458 4572
Size: 180 605 70 50 105 1149 327 114
Cuts at: 4572 4724 5074 5269 5636 6021 6038 7490 7576
Size: 152 350 195 367 385 17 1452 86
Cuts at: 7576 7588 7934 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1149 605 385 367 350 346
327 195 180 152 114 105 86 70
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2200 2454 2592 2790 4719 4962
Size: 552 939 196 254 138 198 1929 243
Cuts at: 4962 513
Size: 3490
Fragments arranged by size:
3490 1929 939 552 254 243 198 196
138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7200
Fragments arranged by size:
7200 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 2586 3217 5060 5455 6249 6269 2466
Size: 120 631 1843 395 794 20 4136
Fragments arranged by size:
4136 1843 794 631 395 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7904
DraI TTT'AAA
Cuts at: 5243 5243
Size: 7939
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6946 809
Size: 507 5630 1802
Fragments arranged by size:
5630 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2663 2793 2886
Size: 54 1425 50 275 18 833 130 93
Cuts at: 2886 5126 5386 6275 7904 8
Size: 2240 260 889 1629 43
Fragments arranged by size:
2240 1629 1425 889 833 275 260 130
93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2663 5386 1537
Size: 275 851 2723 4090
Fragments arranged by size:
4090 2723 851 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2486 2748 3338 3572 3974 4592 5142 5869
Size: 1133 262 590 234 402 618 550 727
Cuts at: 5869 1353
Size: 3423
Fragments arranged by size:
3423 1133 727 618 590 550 402 262
234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6526 6900 7046 387
Size: 1064 138 4937 374 146 1280
Fragments arranged by size:
4937 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3952 3952
Size: 7939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2497 2704 2800 2990 3026 3407 3589
Size: 393 865 207 96 190 36 381 182
Cuts at: 3589 3976 4292 4414 5018 5166 6002 6599 7386
Size: 387 316 122 604 148 836 597 787
Cuts at: 7386 7654 1239
Size: 268 1524
Fragments arranged by size:
1524 865 836 787 604 597 393 387
381 316 268 207 190 182 148 122
96 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2843 3008 3324 2843
Size: 165 316 7458
Fragments arranged by size:
7458 316 165
EcoO109I rG'GnC_Cy
Cuts at: 2029 3101 3382 5105 5140 2029
Size: 1072 281 1723 35 4828
Fragments arranged by size:
4828 1723 1072 281 35
EcoRI G'AATT_C
Cuts at: 2999 4975 5233 5248 5626 2999
Size: 1976 258 15 378 5312
Fragments arranged by size:
5312 1976 378 258 15
EcoRV GAT'ATC
Cuts at: 2707 3252 2707
Size: 545 7394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4921 4921
Size: 7939
FspI TGC'GCA
Cuts at: 6445 6445
Size: 7939
HaeII r_GCGC'y
Cuts at: 1678 7086 1678
Size: 5408 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 3555 3587 3758
Size: 32 135 32 572 32 1379 32 171
Cuts at: 3758 3790 3806 3838 6580 6612 6654 6686 1373
Size: 32 16 32 2742 32 42 32 2626
Fragments arranged by size:
2742 2626 1379 572 171 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 5283 5529 669
Size: 988 281 3345 246 3079
Fragments arranged by size:
3345 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7939
HpaI GTT'AAC
Cuts at: 5529 5529
Size: 7939
KpnI G_GTAC'C
Cuts at: 5264 5264
Size: 7939
MfeI C'AATT_G
Cuts at: 2637 5538 2637
Size: 2901 5038
MluI A'CGCG_T
Cuts at: 7815 7815
Size: 7939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3202 3648 7053 7237 1305
Size: 1897 446 3405 184 2007
Fragments arranged by size:
3405 2007 1897 446 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2888 10
Size: 54 1425 343 1056 5061
Fragments arranged by size:
5061 1425 1056 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2591 4091 4202 4220 5897 6256
Size: 648 1293 132 1500 111 18 1677 359
Cuts at: 6256 6415 518
Size: 159 2042
Fragments arranged by size:
2042 1677 1500 1293 648 359 159 132
111 18
MspA1I CmG'CkG
Cuts at: 1606 2619 2645 4810 4914 5050 5125 5355 6032
Size: 1013 26 2165 104 136 75 230 677
Cuts at: 6032 7180 7425 1606
Size: 1148 245 2120
Fragments arranged by size:
2165 2120 1148 1013 677 245 230 136
104 75 26
NaeI GCC'GGC
Cuts at: 4050 4050
Size: 7939
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 4962 513
Size: 552 939 588 2370 3490
Fragments arranged by size:
3490 2370 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7939
NgoMIV G'CCGG_C
Cuts at: 4048 4048
Size: 7939
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7939
NotI GC'GGCC_GC
Cuts at: 5386 5386
Size: 7939
NsiI A_TGCA'T
Cuts at: 4088 4088
Size: 7939
NspI r_CATG'y
Cuts at: 1132 1229 2768 4438 5297 6842 1132
Size: 97 1539 1670 859 1545 2229
Fragments arranged by size:
2229 1670 1545 1539 859 97
PasI CC'CwG_GG
Cuts at: 1348 4321 1348
Size: 2973 4966
PciI A'CATG_T
Cuts at: 6838 6838
Size: 7939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2767 3503 4270 1108
Size: 1659 736 767 4777
Fragments arranged by size:
4777 1659 767 736
PfoI T'CCnGG_A
Cuts at: 2994 4385 5085 2994
Size: 1391 700 5848
Fragments arranged by size:
5848 1391 700
PmeI GTTT'AAAC
Cuts at: 5243 5243
Size: 7939
PmlI CAC'GTG
Cuts at: 2941 4296 2941
Size: 1355 6584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 6008 6040 7521 7553 3776
Size: 32 2200 32 1481 32 4162
Fragments arranged by size:
4162 2200 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 3101 3382 5105 5140 2029
Size: 1072 281 1723 35 4828
Fragments arranged by size:
4828 1723 1072 281 35
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7939
PsiI TTA'TAA
Cuts at: 5509 5509
Size: 7939
PstI C_TGCA'G
Cuts at: 830 3632 4176 5055 5291 830
Size: 2802 544 879 236 3478
Fragments arranged by size:
3478 2802 879 544 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7939
PvuII CAG'CTG
Cuts at: 2645 2645
Size: 7939
SacI G_AGCT'C
Cuts at: 3954 3954
Size: 7939
SalI G'TCGA_C
Cuts at: 1936 5281 1936
Size: 3345 4594
SapI GCTCTTCn'nnn_
Cuts at: 2486 3338 2486
Size: 852 7087
SbfI CC_TGCA'GG
Cuts at: 5291 5291
Size: 7939
ScaI AGT'ACT
Cuts at: 2741 2933 6187 7899 2741
Size: 192 3254 1712 2781
Fragments arranged by size:
3254 2781 1712 192
SexAI A'CCwGG_T
Cuts at: 3456 3456
Size: 7939
SfcI C'TryA_G
Cuts at: 826 1045 3628 4172 5051 5287 6422 7103 7294
Size: 219 2583 544 879 236 1135 681 191
Cuts at: 7294 826
Size: 1471
Fragments arranged by size:
2583 1471 1135 879 681 544 236 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2894 2894
Size: 7939
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7939
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7939
SmaI CCC'GGG
Cuts at: 1872 4661 5266 1872
Size: 2789 605 4545
Fragments arranged by size:
4545 2789 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2831 4249 6043 6944 7206 820
Size: 197 926 888 1418 1794 901 262 1553
Fragments arranged by size:
1794 1553 1418 926 901 888 262 197
SnaBI TAC'GTA
Cuts at: 493 5730 493
Size: 5237 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7939
SphI G_CATG'C
Cuts at: 1132 4438 5297 1132
Size: 3306 859 3774
Fragments arranged by size:
3774 3306 859
SrfI GCCC'GGGC
Cuts at: 4661 4661
Size: 7939
SspI AAT'ATT
Cuts at: 5 52 5863 5
Size: 47 5811 2081
Fragments arranged by size:
5811 2081 47
StuI AGG'CCT
Cuts at: 1517 2324 2897 1517
Size: 807 573 6559
Fragments arranged by size:
6559 807 573
StyI C'CwwG_G
Cuts at: 513 1065 2004 2104 2325 2592 3048 3804 4708
Size: 552 939 100 221 267 456 756 904
Cuts at: 4708 4962 5378 513
Size: 254 416 3074
Fragments arranged by size:
3074 939 904 756 552 456 416 267
254 221 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 3887 3940 5102 5966 5983 6136
Size: 1255 1037 1270 53 1162 864 17 153
Cuts at: 6136 6321 6766 325
Size: 185 445 1498
Fragments arranged by size:
1498 1270 1255 1162 1037 864 445 185
153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 2931 3188
Size: 274 80 33 51 1443 762 192 257
Cuts at: 3188 3264 6185 7897 96
Size: 76 2921 1712 138
Fragments arranged by size:
2921 1712 1443 762 274 257 192 138
80 76 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4292 4324 4976 5008 7899 7931 4016
Size: 32 244 32 652 32 2891 32 4024
Fragments arranged by size:
4024 2891 652 244 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7939
XbaI T'CTAG_A
Cuts at: 5275 5275
Size: 7939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2278 2803 3768 4052 2278
Size: 525 965 284 6165
Fragments arranged by size:
6165 965 525 284
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7939
XmaI C'CCGG_G
Cuts at: 1870 4659 5264 1870
Size: 2789 605 4545
Fragments arranged by size:
4545 2789 605
XmnI GAAnn'nnTTC
Cuts at: 2540 6068 2540
Size: 3528 4411
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5747 276
Size: 53 83 186 936 4213 2468
Fragments arranged by size:
4213 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I
Bsu36I DraIII FseI FspAI KasI NarI NruI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SfoI SwaI