Restriction map for
pFN21AE9545
(Linear) (Six-base) MAPSORT of: KIEE9545.seq Check: 2818 from: 1 to: 3234
KIEE9545.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1416 1687 2347 3234
Size: 1416 271 660 887
Fragments arranged by size:
1416 887 660 271
AccI GT'mk_AC
Cuts at: 0 1271 3234
Size: 1271 1963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 985 1021 1402 2409 3161 3234
Size: 699 286 36 381 1007 752 73
Fragments arranged by size:
1007 752 699 381 286 73 36
AhdI GACnn_n'nnGTC
Cuts at: 0 1767 3234
Size: 1767 1467
AleI CACnn'nnGTG
Cuts at: 0 454 3234
Size: 454 2780
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 2215 3234
Size: 68 2147 1019
Fragments arranged by size:
2147 1019 68
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 3234
Size: 1771 32 1431
Fragments arranged by size:
1771 1431 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1380 1742 2175 2265 2805 2841 3234
Size: 1380 362 433 90 540 36 393
Fragments arranged by size:
1380 540 433 393 362 90 36
ApoI r'AATT_y
Cuts at: 0 673 780 994 2970 3228 3234
Size: 673 107 214 1976 258 6
Fragments arranged by size:
1976 673 258 214 107 6
AvaI C'yCGr_G
Cuts at: 0 2654 2779 3234
Size: 2654 125 455
Fragments arranged by size:
2654 455 125
BamHI G'GATC_C
Cuts at: 0 2719 3234
Size: 2719 515
BanI G'GyrC_C
Cuts at: 0 946 1481 1608 3234
Size: 946 535 127 1626
Fragments arranged by size:
1626 946 535 127
BanII G_rGCy'C
Cuts at: 0 78 110 476 833 1328 1676 1832 1949
Size: 78 32 366 357 495 348 156 117
Cuts at: 1949 2015 2162 2305 3234
Size: 66 147 143 929
Fragments arranged by size:
929 495 366 357 348 156 147 143
117 78 66 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 2768 3234
Size: 2768 466
BbvCI CC'TCA_GC
Cuts at: 0 520 689 3234
Size: 520 169 2545
Fragments arranged by size:
2545 520 169
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2043 2077 2586 2620 3234
Size: 2043 34 509 34 614
Fragments arranged by size:
2043 614 509 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 274 3234
Size: 274 2960
BfrBI ATG'CAT
Cuts at: 0 2081 3234
Size: 2081 1153
BglI GCCn_nnn'nGGC
Cuts at: 0 889 2300 2524 2708 2784 3234
Size: 889 1411 224 184 76 450
Fragments arranged by size:
1411 889 450 224 184 76
BglII A'GATC_T
Cuts at: 0 147 822 872 2567 3069 3234
Size: 147 675 50 1695 502 165
Fragments arranged by size:
1695 675 502 165 147 50
Bme1580I G_kGCm'C
Cuts at: 0 631 1613 1756 3234
Size: 631 982 143 1478
Fragments arranged by size:
1478 982 631 143
BmgBI CAC'GTC
Cuts at: 0 2027 3234
Size: 2027 1207
BmrI ACTGGGnnnn_n'
Cuts at: 0 630 726 3234
Size: 630 96 2508
Fragments arranged by size:
2508 630 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1518 1576 3234
Size: 1518 58 1658
Fragments arranged by size:
1658 1518 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 795 1584 1971 2287 3013 3234
Size: 492 303 789 387 316 726 221
Fragments arranged by size:
789 726 492 387 316 303 221
Bpu10I CC'TnA_GC
Cuts at: 0 438 520 689 3234
Size: 438 82 169 2545
Fragments arranged by size:
2545 438 169 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 2229 3234
Size: 847 1382 1005
Fragments arranged by size:
1382 1005 847
BsaI GGTCTCn'nnnn_
Cuts at: 0 1207 3107 3234
Size: 1207 1900 127
Fragments arranged by size:
1900 1207 127
BsaAI yAC'GTr
Cuts at: 0 936 2291 3234
Size: 936 1355 943
Fragments arranged by size:
1355 943 936
BsaBI GATnn'nnATC
Cuts at: 0 146 2209 2518 2572 3234
Size: 146 2063 309 54 662
Fragments arranged by size:
2063 662 309 146 54
BsaHI Gr'CG_yC
Cuts at: 0 443 3234
Size: 443 2791
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1771 1795 1801 1825 2287 2317 2971 3001
Size: 1771 24 6 24 462 30 654 30
Cuts at: 3001 3234
Size: 233
Fragments arranged by size:
1771 654 462 233 30 30 24 24
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 495 508 894 1588 1948 1951 2623
Size: 250 245 13 386 694 360 3 672
Cuts at: 2623 2721 2724 3234
Size: 98 3 510
Fragments arranged by size:
694 672 510 386 360 250 245 98
13 3 3
BseYI C'CCAG_C
Cuts at: 0 20 252 276 468 970 1863 1890 2816
Size: 20 232 24 192 502 893 27 926
Cuts at: 2816 3153 3234
Size: 337 81
Fragments arranged by size:
926 893 502 337 232 192 81 27
24 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 2175 3234
Size: 1367 808 1059
Fragments arranged by size:
1367 1059 808
BsiEI CG_ry'CG
Cuts at: 0 661 3234
Size: 661 2573
BsiHKAI G_wGCw'C
Cuts at: 0 401 575 1949 2069 3234
Size: 401 174 1374 120 1165
Fragments arranged by size:
1374 1165 401 174 120
BsmBI CGTCTCn'nnnn_
Cuts at: 0 450 2685 3234
Size: 450 2235 549
Fragments arranged by size:
2235 549 450
Bsp1286I G_dGCh'C
Cuts at: 0 78 110 401 476 575 631 833 1328
Size: 78 32 291 75 99 56 202 495
Cuts at: 1328 1613 1676 1756 1832 1949 2015 2069 2162
Size: 285 63 80 76 117 66 54 93
Cuts at: 2162 2305 3234
Size: 143 929
Fragments arranged by size:
929 495 291 285 202 143 117 99
93 80 78 76 75 66 63 56
54 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1315 1365 1416 1687 2174 2347 3234
Size: 1315 50 51 271 487 173 887
Fragments arranged by size:
1315 887 487 271 173 51 50
BsrDI GCAATG_nn'
Cuts at: 0 1277 1793 3234
Size: 1277 516 1441
Fragments arranged by size:
1441 1277 516
BsrFI r'CCGG_y
Cuts at: 0 2043 3234
Size: 2043 1191
BsrGI T'GTAC_A
Cuts at: 0 1183 3234
Size: 1183 2051
BssSI C'ACGA_G
Cuts at: 0 2296 3234
Size: 2296 938
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2078 2175 3234
Size: 2078 97 1059
Fragments arranged by size:
2078 1059 97
BstEII G'GTnAC_C
Cuts at: 0 1428 1447 2916 3234
Size: 1428 19 1469 318
Fragments arranged by size:
1469 1428 318 19
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 588 940 3234
Size: 456 132 352 2294
Fragments arranged by size:
2294 456 352 132
BstYI r'GATC_y
Cuts at: 0 147 752 822 872 977 2126 2453 2567
Size: 147 605 70 50 105 1149 327 114
Cuts at: 2567 2719 3069 3234
Size: 152 350 165
Fragments arranged by size:
1149 605 350 327 165 152 147 114
105 70 50
BtgI C'CryG_G
Cuts at: 0 195 449 587 785 2714 2957 3234
Size: 195 254 138 198 1929 243 277
Fragments arranged by size:
1929 277 254 243 198 195 138
BtsI GCAGTG_nn'
Cuts at: 0 461 581 1212 3055 3234
Size: 461 120 631 1843 179
Fragments arranged by size:
1843 631 461 179 120
EaeI y'GGCC_r
Cuts at: 0 658 788 881 3121 3234
Size: 658 130 93 2240 113
Fragments arranged by size:
2240 658 130 113 93
EagI C'GGCC_G
Cuts at: 0 658 3234
Size: 658 2576
EarI CTCTTCn'nnn_
Cuts at: 0 481 743 1333 1567 1969 2587 3137 3234
Size: 481 262 590 234 402 618 550 97
Fragments arranged by size:
618 590 550 481 402 262 234 97
EcoICRI GAG'CTC
Cuts at: 0 1947 3234
Size: 1947 1287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 699 795 985 1021 1402 1584 1971
Size: 492 207 96 190 36 381 182 387
Cuts at: 1971 2287 2409 3013 3161 3234
Size: 316 122 604 148 73
Fragments arranged by size:
604 492 387 381 316 207 190 182
148 122 96 73 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 838 1003 1319 3234
Size: 838 165 316 1915
Fragments arranged by size:
1915 838 316 165
EcoO109I rG'GnC_Cy
Cuts at: 0 24 1096 1377 3100 3135 3234
Size: 24 1072 281 1723 35 99
Fragments arranged by size:
1723 1072 281 99 35 24
EcoRI G'AATT_C
Cuts at: 0 994 2970 3228 3234
Size: 994 1976 258 6
Fragments arranged by size:
1976 994 258 6
EcoRV GAT'ATC
Cuts at: 0 702 1247 3234
Size: 702 545 1987
Fragments arranged by size:
1987 702 545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2916 3234
Size: 2916 318
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 1550 1582 1753 1785 1801 1833
Size: 139 32 1379 32 171 32 16 32
Cuts at: 1833 3234
Size: 1401
Fragments arranged by size:
1401 1379 171 139 32 32 32 32
16
MfeI C'AATT_G
Cuts at: 0 632 3234
Size: 632 2602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 1643 3234
Size: 1197 446 1591
Fragments arranged by size:
1591 1197 446
MscI TGG'CCA
Cuts at: 0 883 3234
Size: 883 2351
MslI CAynn'nnrTG
Cuts at: 0 454 586 2086 2197 2215 3234
Size: 454 132 1500 111 18 1019
Fragments arranged by size:
1500 1019 454 132 111 18
MspA1I CmG'CkG
Cuts at: 0 614 640 2805 2909 3045 3120 3234
Size: 614 26 2165 104 136 75 114
Fragments arranged by size:
2165 614 136 114 104 75 26
NaeI GCC'GGC
Cuts at: 0 2045 3234
Size: 2045 1189
NcoI C'CATG_G
Cuts at: 0 587 2957 3234
Size: 587 2370 277
Fragments arranged by size:
2370 587 277
NgoMIV G'CCGG_C
Cuts at: 0 2043 3234
Size: 2043 1191
NsiI A_TGCA'T
Cuts at: 0 2083 3234
Size: 2083 1151
NspI r_CATG'y
Cuts at: 0 763 2433 3234
Size: 763 1670 801
Fragments arranged by size:
1670 801 763
PasI CC'CwG_GG
Cuts at: 0 2316 3234
Size: 2316 918
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1498 2265 3234
Size: 762 736 767 969
Fragments arranged by size:
969 767 762 736
PfoI T'CCnGG_A
Cuts at: 0 989 2380 3080 3234
Size: 989 1391 700 154
Fragments arranged by size:
1391 989 700 154
PmlI CAC'GTG
Cuts at: 0 936 2291 3234
Size: 936 1355 943
Fragments arranged by size:
1355 943 936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1771 1803 3234
Size: 1771 32 1431
Fragments arranged by size:
1771 1431 32
PpuMI rG'GwC_Cy
Cuts at: 0 24 1096 1377 3100 3135 3234
Size: 24 1072 281 1723 35 99
Fragments arranged by size:
1723 1072 281 99 35 24
PstI C_TGCA'G
Cuts at: 0 1627 2171 3050 3234
Size: 1627 544 879 184
Fragments arranged by size:
1627 879 544 184
PvuII CAG'CTG
Cuts at: 0 640 3234
Size: 640 2594
SacI G_AGCT'C
Cuts at: 0 1949 3234
Size: 1949 1285
SapI GCTCTTCn'nnn_
Cuts at: 0 481 1333 3234
Size: 481 852 1901
Fragments arranged by size:
1901 852 481
ScaI AGT'ACT
Cuts at: 0 736 928 3234
Size: 736 192 2306
Fragments arranged by size:
2306 736 192
SexAI A'CCwGG_T
Cuts at: 0 1451 3234
Size: 1451 1783
SfcI C'TryA_G
Cuts at: 0 1623 2167 3046 3234
Size: 1623 544 879 188
Fragments arranged by size:
1623 879 544 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 889 3234
Size: 889 2345
SmaI CCC'GGG
Cuts at: 0 2656 3234
Size: 2656 578
SmlI C'TyrA_G
Cuts at: 0 826 2244 3234
Size: 826 1418 990
Fragments arranged by size:
1418 990 826
SphI G_CATG'C
Cuts at: 0 2433 3234
Size: 2433 801
SrfI GCCC'GGGC
Cuts at: 0 2656 3234
Size: 2656 578
StuI AGG'CCT
Cuts at: 0 319 892 3234
Size: 319 573 2342
Fragments arranged by size:
2342 573 319
StyI C'CwwG_G
Cuts at: 0 99 320 587 1043 1799 2703 2957 3234
Size: 99 221 267 456 756 904 254 277
Fragments arranged by size:
904 756 456 277 267 254 221 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 1882 1935 3097 3234
Size: 612 1270 53 1162 137
Fragments arranged by size:
1270 1162 612 137 53
TatI w'GTAC_w
Cuts at: 0 734 926 1183 1259 3234
Size: 734 192 257 76 1975
Fragments arranged by size:
1975 734 257 192 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2011 2043 2287 2319 2971 3003 3234
Size: 2011 32 244 32 652 32 231
Fragments arranged by size:
2011 652 244 231 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 798 1763 2047 3234
Size: 273 525 965 284 1187
Fragments arranged by size:
1187 965 525 284 273
XmaI C'CCGG_G
Cuts at: 0 2654 3234
Size: 2654 580
XmnI GAAnn'nnTTC
Cuts at: 0 535 3234
Size: 535 2699
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AloI AlwNI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NsiI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI
SapI ScaI SexAI SfcI SfiI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BclI
BlpI BmtI BsaWI BsiWI BsmI BspEI BspHI BsrBI
BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI PacI PciI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SfoI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI ZraI