Restriction map for pFN21AE1779
 (Circular) (Six-base) MAPSORT of: pFN21AE1779.seq  Check: 5787  from: 1  to: 5569



pFN21AE1779  5569 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3379     278 
   Size:         53      83     186     936    1843    2468

  Fragments arranged by size: 

                2468    1843     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2912    1937 
   Size:        975    4594
 
Acc65I G'GTAC_C

Cuts at:   2890    2890 
   Size:       5569
 
AclI AA'CG_TT

Cuts at:   1563    3696    4069    1563 
   Size:       2133     373    3063

  Fragments arranged by size: 

                3063    2133     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2381    2421    3632    5016    5284    1632 
   Size:        749      40    1211    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1211     749     268      40
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5372
 
AflIII A'CryG_T

Cuts at:   1203    4468    5445    1203 
   Size:       3265     977    1327

  Fragments arranged by size: 

                3265    1327     977
 
AgeI A'CCGG_T

Cuts at:   4400    4400 
   Size:       5569
 
AhdI GACnn_n'nnGTC

Cuts at:   4298    4298 
   Size:       5569
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5569
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5569
 
AlwNI CAG_nnn'CTG

Cuts at:   3312    4884    3312 
   Size:       1572    3997
 
ApaLI G'TGCA_C

Cuts at:   3626    4782    3626 
   Size:       1156    4413
 
ApoI r'AATT_y

Cuts at:   1493    2457    2772    2878    3104    3256    5365    1493 
   Size:        964     315     106     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     964     315     226     152     106
 
AseI AT'TA_AT

Cuts at:    160    1032    4123     160 
   Size:        872    3091    1606

  Fragments arranged by size: 

                3091    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5569
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2258    2894    1870 
   Size:         73     315     636    4545

  Fragments arranged by size: 

                4545     636     315      73
 
BamHI G'GATC_C

Cuts at:   2899    3266    2899 
   Size:        367    5202
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2890    4345     618 
   Size:        325     217     627    1103    1455    1842

  Fragments arranged by size: 

                1842    1455    1103     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2595    2888    1391 
   Size:       1204     293    4072

  Fragments arranged by size: 

                4072    1204     293
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4598
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3760    3794    1903 
   Size:         34    1823      34    3678

  Fragments arranged by size: 

                3678    1823      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3461    4671    3461 
   Size:       1210    4359
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5569
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4180     136 
   Size:        107     122      71     631     605    2508    1525

  Fragments arranged by size: 

                2508    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5564    5564 
   Size:       5569
 
BlpI GC'TnA_GC

Cuts at:   2986    2986 
   Size:       5569
 
Bme1580I G_kGCm'C

Cuts at:   1790    2342    3630    4786    1790 
   Size:        552    1288    1156    2573

  Fragments arranged by size: 

                2573    1288    1156     552
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5569
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3300    4258     442 
   Size:        416      53      83     399      24    1883     958    1753

  Fragments arranged by size: 

                1883    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5569
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2520    4229    1239 
   Size:       1281    1709    2579

  Fragments arranged by size: 

                2579    1709    1281
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3694    4559    4857    3694 
   Size:        865     298    4406

  Fragments arranged by size: 

                4406     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4232     882 
   Size:        617    2733    2219

  Fragments arranged by size: 

                2733    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2116    3360     493 
   Size:        711     912    1244    2702

  Fragments arranged by size: 

                2702    1244     912     711
 
BsaBI GATnn'nnATC

Cuts at:   5563    5563 
   Size:       5569
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3376    3758    4393     275 
   Size:         53      83     186     936    1843     382     635    1451

  Fragments arranged by size: 

                1843    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2271    4002    4400    4674    4821    5232    2271 
   Size:       1731     398     274     147     411    2608

  Fragments arranged by size: 

                2608    1731     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5344

  Fragments arranged by size: 

                5344     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5569
 
BseYI C'CCAG_C

Cuts at:   4772    5268    4772 
   Size:        496    5073
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4996
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3019    3780    4808    1540 
   Size:        275     186    1018     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1018     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2595    3630    3715    4786    2595 
   Size:       1035      85    1071    3378

  Fragments arranged by size: 

                3378    1071    1035      85
 
BsmI GAATG_Cn'

Cuts at:   3080    3173    3080 
   Size:         93    5476
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2342    2595    2888    3630    3715    4786    1391 
   Size:        399     552     253     293     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     552     399     293     253      85
            
 
BspEI T'CCGG_A

Cuts at:   5232    5232 
   Size:       5569
 
BspHI T'CATG_A

Cuts at:   3458    5188    3458 
   Size:       1730    3839
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2739    2924     844 
   Size:       1895     185    3489

  Fragments arranged by size: 

                3489    1895     185
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3456    1394 
   Size:        429    1633    3507

  Fragments arranged by size: 

                3507    1633     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4064    4238      65 
   Size:       1183    2816     174    1396

  Fragments arranged by size: 

                2816    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4213    4400    1700 
   Size:        110    2403     187    2869

  Fragments arranged by size: 

                2869    2403     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5569
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5569
 
BssSI C'ACGA_G

Cuts at:   3629    4641    3629 
   Size:       1012    4557
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5569
 
BstBI TT'CG_AA

Cuts at:   4373    4373 
   Size:       5569
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5569
 
BstYI r'GATC_y

Cuts at:   1972    2578    2899    3266    3651    3668    5120    5206    5218 
   Size:        606     321     367     385      17    1452      86      12

Cuts at:   5218    5564    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     606     385     367     346     321      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4078

  Fragments arranged by size: 

                4078     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4830

  Fragments arranged by size: 

                4830     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3085    3879    3899    3085 
   Size:        794      20    4755

  Fragments arranged by size: 

                4755     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5569
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5534
 
DraI TTT'AAA

Cuts at:   2873    2873 
   Size:       5569
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2233    4576     809 
   Size:        507     917    2343    1802

  Fragments arranged by size: 

                2343    1802     917     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3016    3905    5534 
   Size:         54    1425      50     275      18    1186     889    1629

Cuts at:   5534       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1186     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3016    1537 
   Size:        275    1204    4090

  Fragments arranged by size: 

                4090    1204     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2169    3499    1353 
   Size:        816    1330    3423

  Fragments arranged by size: 

                3423    1330     816
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4156    4530    4676     387 
   Size:       1064     138    2567     374     146    1280

  Fragments arranged by size: 

                2567    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2593    2593 
   Size:       5569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2381    2421    2520    3632    4229    5016    5284 
   Size:        393     749      40      99    1112     597     787     268

Cuts at:   5284    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1112     787     749     597     393     268      99
                  40
 
EcoRI G'AATT_C

Cuts at:   2878    3256    2878 
   Size:        378    5191
 
EcoRV GAT'ATC

Cuts at:   2287    2287 
   Size:       5569
 
FspI TGC'GCA

Cuts at:   4075    4075 
   Size:       5569
 
HaeII r_GCGC'y

Cuts at:   1678    4716    1678 
   Size:       3038    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2302    2334    4210    4242    4284 
   Size:         32     135      32     730      32    1876      32      42

Cuts at:   4284    4316    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1876     730     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2913    3159     669 
   Size:        988     281     975     246    3079

  Fragments arranged by size: 

                3079     988     975     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5569
 
HpaI GTT'AAC

Cuts at:   3159    3159 
   Size:       5569
 
KpnI G_GTAC'C

Cuts at:   2894    2894 
   Size:       5569
 
MfeI C'AATT_G

Cuts at:   3168    3168 
   Size:       5569
 
MluI A'CGCG_T

Cuts at:   5445    5445 
   Size:       5569
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2486    2511    4683    4867    1305 
   Size:       1181      25    2172     184    2007

  Fragments arranged by size: 

                2172    2007    1181     184      25
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3747

  Fragments arranged by size: 

                3747    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2041    2347    3527    3886    4045     518 
   Size:        648     875     306    1180     359     159    2042

  Fragments arranged by size: 

                2042    1180     875     648     359     306     159
 
MspA1I CmG'CkG

Cuts at:   1606    2985    3662    4810    5055    1606 
   Size:       1379     677    1148     245    2120

  Fragments arranged by size: 

                2120    1379    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4078

  Fragments arranged by size: 

                4078     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5569
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5569
 
NotI GC'GGCC_GC

Cuts at:   3016    3016 
   Size:       5569
 
NspI r_CATG'y

Cuts at:   1132    1229    2927    4472    1132 
   Size:         97    1698    1545    2229

  Fragments arranged by size: 

                2229    1698    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5569
 
PciI A'CATG_T

Cuts at:   4468    4468 
   Size:       5569
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5569
 
PmeI GTTT'AAAC

Cuts at:   2873    2873 
   Size:       5569
 
PmlI CAC'GTG

Cuts at:   2116    2116 
   Size:       5569
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3638    3670    5151    5183    3638 
   Size:         32    1481      32    4024

  Fragments arranged by size: 

                4024    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5569
 
PsiI TTA'TAA

Cuts at:   3139    3139 
   Size:       5569
 
PstI C_TGCA'G

Cuts at:    830    2921     830 
   Size:       2091    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5569
 
SacI G_AGCT'C

Cuts at:   2595    2595 
   Size:       5569
 
SalI G'TCGA_C

Cuts at:   1936    2911    1936 
   Size:        975    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2169    2169 
   Size:       5569
 
SbfI CC_TGCA'GG

Cuts at:   2921    2921 
   Size:       5569
 
ScaI AGT'ACT

Cuts at:   3817    5529    3817 
   Size:       1712    3857
 
SfcI C'TryA_G

Cuts at:    826    1045    2865    2917    4052    4733    4924     826 
   Size:        219    1820      52    1135     681     191    1471

  Fragments arranged by size: 

                1820    1471    1135     681     219     191      52
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5569
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5569
 
SmaI CCC'GGG

Cuts at:   1872    2896    1872 
   Size:       1024    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3673    4574    4836     820 
   Size:        197     926    1730     901     262    1553

  Fragments arranged by size: 

                1730    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3360     493 
   Size:       2867    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5569
 
SphI G_CATG'C

Cuts at:   1132    2927    1132 
   Size:       1795    3774
 
SspI AAT'ATT

Cuts at:      5      52    2473    3493       5 
   Size:         47    2421    1020    2081

  Fragments arranged by size: 

                2421    2081    1020      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5569
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2121    3008     513 
   Size:        552     939     117     887    3074

  Fragments arranged by size: 

                3074     939     887     552     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3596    3613    3766    3951    4396     325 
   Size:       1255    2016      17     153     185     445    1498

  Fragments arranged by size: 

                2016    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2262    3815    5527 
   Size:        274      80      33      51    1443     285    1553    1712

Cuts at:   5527      96 
   Size:        138

  Fragments arranged by size: 

                1712    1553    1443     285     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2763    2795    5529    5561    2763 
   Size:         32    2734      32    2771

  Fragments arranged by size: 

                2771    2734      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5569
 
XbaI T'CTAG_A

Cuts at:   2905    2905 
   Size:       5569
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2311    2311 
   Size:       5569
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5569
 
XmaI C'CCGG_G

Cuts at:   1870    2894    1870 
   Size:       1024    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3698    3698 
   Size:       5569
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3377     276 
   Size:         53      83     186     936    1843    2468

  Fragments arranged by size: 

                2468    1843     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI     PstI     PvuI     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII    EcoNI EcoO109I     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI
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