################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HhaI HinP1I HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C AfaI 68 GTAC GT^AC BamHI 348 GGATCC G^GATCC BbsI 213 GAAGAC GAAGAC(2/6) BclI 422 TGATCA T^GATCA BpmI 296 CTGGAG CTGGAG(16/14) BpuAI 213 GAAGAC GAAGAC(2/6) BsaHI 3 GGCGTC G[GA]^CG[CT]C BsaJI 26 CCGTGG C^C[ACGT][ACGT]GG BsmFI 6 GTCCC GGGAC(10/14) BsrDI 301 GCAATG GCAATG(2/0) BssKI 477 CCAGG ^CC[ACGT]GG BstF5I 30 GGATG GGATG(2/0) BstNI 477 CCAGG CC^[AT]GG BstYI 348 GGATCC [GA]^GATC[CT] DdeI 594 CTCAG C^T[ACGT]AG DsaI 26 CCGTGG C^C[GA][CT]GG EcoRI 276 GAATTC G^AATTC EcoRII 477 CCAGG ^CC[AT]GG FbaI 422 TGATCA T^GATCA FokI 30 GGATG GGATG(9/13) HgaI 4 GCGTC GACGC(5/10) Hin1I 3 GGCGTC G[GA]^CG[CT]C HincII 334 GTTAAC GT[CT]^[GA]AC HindII 334 GTTAAC GT[CT]^[GA]AC HpaI 334 GTTAAC GTT^AAC MflI 348 GGATCC [GA]^GATC[CT] MslI 396 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 477 CCAGG CC^[AT]GG MwoI 541 GCTCCATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 396 CATG CATG^ NlaIV 348 GGATCC GG[ACGT]^[ACGT]CC RsaI 68 GTAC GT^AC ScrFI 477 CCAGG CC^[ACGT]GG SfaNI 198 GCATC GCATC(5/9) SmlI 619 CTCAAG C^T[CT][GA]AG XhoII 348 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 255 GTCTAC GT^[AC][GT]AC 306 GTCTAC AlwI 349 GATCC GGATC(4/5) 348 GGATC BalI 146 TGGCCA TGG^CCA 533 TGGCCA BbvI 97 GCAGC GCAGC(8/12) 550 GCTGC Cac8I 169 GCTTGC GC[ACGT]^[ACGT]GC 327 GCAGGC EaeI 146 TGGCCA [CT]^GGCC[GA] 533 TGGCCA EarI 10 CTCTTC CTCTTC(1/4) 601 GAAGAG Fnu4HI 97 GCAGC GC^[ACGT]GC 550 GCTGC HaeIII 147 GGCC GG^CC 534 GGCC HindIII 167 AAGCTT A^AGCTT 558 AAGCTT HinfI 51 GAATC G^A[ACGT]TC 489 GAATC ItaI 97 GCAGC GC^[ACGT]GC 550 GCTGC Ksp632I 10 CTCTTC CTCTTC(1/4) 601 GAAGAG MaeIII 60 GTTAC ^GT[ACGT]AC 244 GTTAC MluNI 146 TGGCCA TGG^CCA 533 TGGCCA MscI 146 TGGCCA TGG^CCA 533 TGGCCA MseI 137 TTAA T^TAA 335 TTAA MspA1I 121 CAGCTG C[AC]G^C[GT]G 548 CAGCTG PvuII 121 CAGCTG CAG^CTG 548 CAGCTG TfiI 51 GAATC G^A[AT]TC 489 GAATC Tru9I 137 TTAA T^TAA 335 TTAA TseI 97 GCAGC G^C[AT]GC 550 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AcsI AluI ApoI DpnII MboI MboII MnlI NdeII Sau3AI TaqI Tsp509I TthHB8I