################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeIII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 262 GACGTC GACGT^C AcyI 262 GACGTC G[GA]^CG[CT]C AluI 325 AGCT AG^CT AlwI 198 GATCC GGATC(4/5) Aor51HI 284 AGCGCT AGC^GCT AvaI 45 CCCGGG C^[CT]CG[GA]G BcgI 54 CGATCGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 505 CTAG C^TAG BpmI 442 CTGGAG CTGGAG(16/14) BsaBI 396 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 262 GACGTC G[GA]^CG[CT]C BseRI 350 GAGGAG GAGGAG(10/8) BsmAI 417 GAGAC GTCTC(1/5) BsoBI 45 CCCGGG C^[CT]CG[GA]G BspMI 116 ACCTGC ACCTGC(4/8) BsrI 441 ACTGG ACTGG(1/-1) BstXI 134 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 322 GCGAGC GC[ACGT]^[ACGT]GC DdeI 181 CTGAG C^T[ACGT]AG EaeI 67 CGGCCG [CT]^GGCC[GA] EagI 67 CGGCCG C^GGCCG EarI 471 GAAGAG CTCTTC(1/4) EclXI 67 CGGCCG C^GGCCG Eco47III 284 AGCGCT AGC^GCT Eco52I 67 CGGCCG C^GGCCG EcoT22I 407 ATGCAT ATGCA^T HaeII 284 AGCGCT [GA]GCGC^[CT] HaeIII 68 GGCC GG^CC Hin1I 262 GACGTC G[GA]^CG[CT]C Ksp632I 471 GAAGAG CTCTTC(1/4) MaeI 505 CTAG C^TAG MamI 396 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 8 TTAA T^TAA MwoI 104 GCGTAGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NsiI 407 ATGCAT ATGCA^T PvuI 54 CGATCG CGAT^CG SexAI 140 ACCTGGT A^CC[AT]GGT SmaI 45 CCCGGG CCC^GGG SmlI 430 CTCAAG C^T[CT][GA]AG TfiI 209 GAATC G^A[AT]TC Tru9I 8 TTAA T^TAA TspRI 537 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 45 CCCGGG C^CCGGG XmaI 45 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 25 CGCG CG^CG 103 CGCG AciI 24 CCGC CCGC(-3/-1) 102 CCGC BbvI 247 GCAGC GCAGC(8/12) 151 GCTGC BsiEI 54 CGATCG CG[GA][CT]^CG 67 CGGCCG BstF5I 275 GGATG GGATG(2/0) 498 GGATG BstUI 25 CGCG CG^CG 103 CGCG CfoI 26 GCGC GCG^C 285 GCGC DpnII 55 GATC ^GATC 198 GATC Fnu4HI 151 GCTGC GC^[ACGT]GC 247 GCAGC FokI 275 GGATG GGATG(9/13) 498 GGATG HhaI 26 GCGC GCG^C 285 GCGC HinP1I 26 GCGC G^CGC 285 GCGC ItaI 151 GCTGC GC^[ACGT]GC 247 GCAGC MboI 55 GATC ^GATC 198 GATC MvnI 25 CGCG CG^CG 103 CGCG NdeII 55 GATC ^GATC 198 GATC NlaIV 49 GGTTCC GG[ACGT]^[ACGT]CC 425 GGAACC Sau3AI 55 GATC ^GATC 198 GATC TseI 151 GCTGC G^C[AT]GC 247 GCAGC XcmI 19 CCACTCCGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 134 CCAACTACCTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### BcnI BsaJI BsiYI BslI BsmFI BssKI BstNI EcoRII HapII HinfI HpaII MaeII MboII MnlI MspI MvaI NciI NlaIII PleI ScrFI SfaNI