################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmFI BspDI BspEI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 601 AAATTC [GA]^AATT[CT] AfaI 177 GTAC GT^AC ApoI 601 AAATTC [GA]^AATT[CT] AspHI 136 GTGCTC G[AT]GC[AT]^C AvaI 111 CTCGAG C^[CT]CG[GA]G AvaII 198 GGACC G^G[AT]CC BcgI 567 GCAGTATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 136 GTGCTC G[AGT]GC[ACT]^C BsaJI 423 CCAGGG C^C[ACGT][ACGT]GG BsaWI 407 TCCGGT [AT]^CCGG[AT] BsiHKAI 136 GTGCTC G[AT]GC[AT]^C BsmBI 100 GAGACG CGTCTC(1/5) BsmI 667 GCATTC GAATGC(1/-1) BsoBI 111 CTCGAG C^[CT]CG[GA]G Bsp1286I 136 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 176 TGTACA T^GTACA BspHI 881 TCATGA T^CATGA BspMI 490 GCAGGT ACCTGC(4/8) BsrBI 807 GAGCGG CCGCTC(-3/-3) BsrDI 608 GCAATG GCAATG(2/0) BsrGI 176 TGTACA T^GTACA Cac8I 617 GCAAGC GC[ACGT]^[ACGT]GC DraII 425 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 425 AGGGCCT [GA]G^G[ACGT]CC[CT] HapII 408 CCGG C^CGG HgaI 35 GCGTC GACGC(5/10) HincII 496 GTCAAC GT[CT]^[GA]AC HindII 496 GTCAAC GT[CT]^[GA]AC HindIII 514 AAGCTT A^AGCTT HpaII 408 CCGG C^CGG MspI 408 CCGG C^CGG NlaIV 543 GGGGCC GG[ACGT]^[ACGT]CC PaeR7I 111 CTCGAG C^TCGAG RcaI 881 TCATGA T^CATGA RsaI 177 GTAC GT^AC SmlI 111 CTCGAG C^T[CT][GA]AG SspBI 176 TGTACA T^GTACA XhoI 111 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 34 CGCG CG^CG 52 CGCG BglI 89 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 149 GCCAGGAGGGC BpmI 743 CTCCAG CTGGAG(16/14) 727 CTGGAG BsaI 374 GGTCTC GGTCTC(1/5) 410 GGTCTC BstUI 34 CGCG CG^CG 52 CGCG BstYI 402 GGATCT [GA]^GATC[CT] 785 AGATCC DdeI 41 CTAAG C^T[ACGT]AG 752 CTCAG EcoT22I 433 ATGCAT ATGCA^T 665 ATGCAT HphI 681 GGTGA GGTGA(8/7) 346 TCACC MflI 402 GGATCT [GA]^GATC[CT] 785 AGATCC MvnI 34 CGCG CG^CG 52 CGCG NsiI 433 ATGCAT ATGCA^T 665 ATGCAT PstI 439 CTGCAG CTGCA^G 488 CTGCAG SfcI 439 CTGCAG C^T[GA][CT]AG 488 CTGCAG Tsp45I 345 GTCAC ^GT[GC]AC 721 GTCAC TspRI 725 CACTG CA[GC]TG[ACGT][ACGT]^ 894 CAGTG XcmI 341 CCATGTCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 789 CCATGTCTGGAATGG XhoII 402 GGATCT [GA]^GATC[CT] 785 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AluI AlwI BbvI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MnlI MseI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TthHB8I