################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 22 CGCG CG^CG ApaI 306 GGGCCC GGGCC^C AspHI 508 GAGCAC G[AT]GC[AT]^C AvaI 384 CCCGGG C^[CT]CG[GA]G AvaII 483 GGACC G^G[AT]CC BalI 194 TGGCCA TGG^CCA BbsI 450 GAAGAC GAAGAC(2/6) BcgI 114 GCATGGTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 450 GAAGAC GAAGAC(2/6) BsiHKAI 508 GAGCAC G[AT]GC[AT]^C BsoBI 384 CCCGGG C^[CT]CG[GA]G BspHI 545 TCATGA T^CATGA BspMI 203 ACCTGC ACCTGC(4/8) BstAPI 235 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 22 CGCG CG^CG BstXI 583 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 21 GCGC GCG^C DdeI 334 CTGAG C^T[ACGT]AG DsaI 488 CCATGG C^C[GA][CT]GG EaeI 194 TGGCCA [CT]^GGCC[GA] EarI 55 CTCTTC CTCTTC(1/4) FauI 556 GCGGG CCCGC(4/6) HhaI 21 GCGC GCG^C HinP1I 21 GCGC G^CGC HinfI 453 GACTC G^A[ACGT]TC Ksp632I 55 CTCTTC CTCTTC(1/4) MaeIII 569 GTTAC ^GT[ACGT]AC MluNI 194 TGGCCA TGG^CCA MscI 194 TGGCCA TGG^CCA MslI 33 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 520 CAGCTG C[AC]G^C[GT]G MvnI 22 CGCG CG^CG NcoI 488 CCATGG C^CATGG NspI 341 GCATGT [GA]CATG^[CT] PleI 453 GACTC GAGTC(4/5) PpuMI 482 AGGACCC [GA]G^G[AT]CC[CT] PstI 616 CTGCAG CTGCA^G PvuII 520 CAGCTG CAG^CTG RcaI 545 TCATGA T^CATGA SbfI 615 CCTGCAGG CCTGCA^GG SfaNI 292 GATGC GCATC(5/9) SfcI 616 CTGCAG C^T[GA][CT]AG SmaI 384 CCCGGG CCC^GGG Sse8387I 615 CCTGCAGG CCTGCA^GG XmaCI 384 CCCGGG C^CCGGG XmaI 384 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 280 GCGG CCGC(-3/-1) 556 GCGG AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 236 CAGCCCCTG BanI 28 GGCACC G^G[CT][GA]CC 560 GGCACC BanII 306 GGGCCC G[GA]GC[CT]^C 622 GGGCTC BclI 320 TGATCA T^GATCA 542 TGATCA BpmI 455 CTCCAG CTGGAG(16/14) 625 CTCCAG BsaI 136 GAGACC GGTCTC(1/5) 476 GAGACC BseRI 129 CTCCTC GAGGAG(10/8) 586 GAGGAG BsmAI 136 GAGAC GTCTC(1/5) 476 GAGAC BsrI 397 CCAGT ACTGG(1/-1) 443 CCAGT BstF5I 33 CATCC GGATG(2/0) 291 GGATG DpnII 321 GATC ^GATC 543 GATC DraIII 332 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 532 CACCGGGTG FbaI 320 TGATCA T^GATCA 542 TGATCA FokI 33 CATCC GGATG(9/13) 291 GGATG MboI 321 GATC ^GATC 543 GATC NdeII 321 GATC ^GATC 543 GATC Sau3AI 321 GATC ^GATC 543 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI BbvI BcnI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI StyI TseI TspRI