Restriction map for
pFN21AE2792
(Circular) (Six-base) MAPSORT of: pFN21AE2792.seq Check: 7509 from: 1 to: 5335
pFN21AE2792 5335 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2540 3398 4782 5050 1632
Size: 908 858 1384 268 1917
Fragments arranged by size:
1917 1384 908 858 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 2155 2246 3078 4650 2155
Size: 91 832 1572 2840
Fragments arranged by size:
2840 1572 832 91
ApaI G_GGCC'C
Cuts at: 2315 2315
Size: 5335
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2389 2660 1870
Size: 73 446 271 4545
Fragments arranged by size:
4545 446 271 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2565 2656 4111 618
Size: 325 217 627 246 532 91 1455 1842
Fragments arranged by size:
1842 1455 627 532 325 246 217 91
BanII G_rGCy'C
Cuts at: 1391 2315 2631 2654 1391
Size: 924 316 23 4072
Fragments arranged by size:
4072 924 316 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 928
Size: 971 563 3801
Fragments arranged by size:
3801 971 563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2108 2142 3526 3560 1903
Size: 34 171 34 1384 34 3678
Fragments arranged by size:
3678 1384 171 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 2325 2547 1548
Size: 777 222 4336
Fragments arranged by size:
4336 777 222
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 136
Size: 107 122 71 631 605 2274 1525
Fragments arranged by size:
2274 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2226 2315 2568 3396 4552 1790
Size: 246 190 89 253 828 1156 2573
Fragments arranged by size:
2573 1156 828 253 246 190 89
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2445 2615 3995 1239
Size: 1206 170 1380 2579
Fragments arranged by size:
2579 1380 1206 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 2475 3998 882
Size: 617 636 340 1523 2219
Fragments arranged by size:
2219 1523 636 617 340
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3142 3524 4159 275
Size: 53 83 186 936 1609 382 635 1451
Fragments arranged by size:
1609 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2606 1186
Size: 939 481 3915
Fragments arranged by size:
3915 939 481
BseYI C'CCAG_C
Cuts at: 2308 2382 4538 5034 2308
Size: 74 2156 496 2609
Fragments arranged by size:
2609 2156 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2150 2401 1163
Size: 573 330 84 251 4097
Fragments arranged by size:
4097 573 330 251 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 3396 3481 4552 2517
Size: 879 85 1071 3300
Fragments arranged by size:
3300 1071 879 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2226 2315 2517 2568 2631 2654
Size: 399 246 190 89 202 51 63 23
Cuts at: 2654 3396 3481 4552 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 246 202 190 89
85 63 51 23
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 2550 3224 4954 2550
Size: 674 1730 2931
Fragments arranged by size:
2931 1730 674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2690 844
Size: 1373 473 3489
Fragments arranged by size:
3489 1373 473
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3979 4166 1700
Size: 110 2169 187 2869
Fragments arranged by size:
2869 2169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2246 794
Size: 1452 3883
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstXI CCAn_nnnn'nTGG
Cuts at: 2595 2595
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330
Size: 693 367 385 17 1452 86 12 346
Cuts at: 5330 1972
Size: 1977
Fragments arranged by size:
1977 1452 693 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2493 513
Size: 552 939 489 3355
Fragments arranged by size:
3355 939 552 489
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2092 2162 2851 3645 3665 2092
Size: 70 689 794 20 3762
Fragments arranged by size:
3762 794 689 70 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DraIII CAC_nnn'GTG
Cuts at: 2342 2542 2342
Size: 200 5135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2782 3671
Size: 54 1425 50 275 18 369 583 889
Cuts at: 3671 5300 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 583 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 3265 1353
Size: 713 1199 3423
Fragments arranged by size:
3423 1199 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3922 4296 4442 387
Size: 1064 138 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2445 2540 2615 3398 3995 4782 5050
Size: 393 813 95 75 783 597 787 268
Cuts at: 5050 1239
Size: 1524
Fragments arranged by size:
1524 813 787 783 597 393 268 95
75
EcoO109I rG'GnC_Cy
Cuts at: 2056 2074 2312 2488 2056
Size: 18 238 176 4903
Fragments arranged by size:
4903 238 176 18
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 4482 1678
Size: 2804 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2449 2481 3976 4008 4050
Size: 32 135 32 877 32 1495 32 42
Cuts at: 4050 4082 1373
Size: 32 2626
Fragments arranged by size:
2626 1495 877 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 10
Size: 54 1425 343 369 3144
Fragments arranged by size:
3144 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 3293 3652 3811 518
Size: 648 876 1251 359 159 2042
Fragments arranged by size:
2042 1251 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2751 3428 4576 4821 1606
Size: 921 224 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 245 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 2493 513
Size: 552 939 489 3355
Fragments arranged by size:
3355 939 552 489
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2350 2693 4238 1132
Size: 97 1121 343 1545 2229
Fragments arranged by size:
2229 1545 1121 343 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PspOMI G'GGCC_C
Cuts at: 2311 2311
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2625 2687 830
Size: 1795 62 3478
Fragments arranged by size:
3478 1795 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
PvuII CAG'CTG
Cuts at: 2527 2527
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2625 2687 2625
Size: 62 5273
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2621 2683 3818 4499 4690 826
Size: 219 1576 62 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 681 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2391 2662 1872
Size: 519 271 4545
Fragments arranged by size:
4545 519 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3439 4340 4602 820
Size: 197 926 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2301 2493 2774 513
Size: 552 939 225 72 192 281 3074
Fragments arranged by size:
3074 939 552 281 225 192 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 2349 3581
Size: 274 80 33 51 1443 130 242 1232
Cuts at: 3581 5293 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1232 274 242 138 130 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2389 2660 1870
Size: 519 271 4545
Fragments arranged by size:
4545 519 271
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI