(Circular) (Six-base) MAPSORT of: pFN21AE3160.seq Check: 8642 from: 1 to: 5845 pFN21AE3160 5845 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2736 3188 1937 Size: 799 452 4594 Fragments arranged by size: 4594 799 452 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2109 3908 5292 5560 1632 Size: 391 86 1799 1384 268 1917 Fragments arranged by size: 1917 1799 1384 391 268 86 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 4129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2900 1184 Size: 1716 4129 AlwNI CAG_nnn'CTG Cuts at: 2016 2683 2764 2917 3588 5160 2016 Size: 667 81 153 671 1572 2701 Fragments arranged by size: 2701 1572 671 667 153 81 ApaLI G'TGCA_C Cuts at: 2437 3902 5058 2437 Size: 1465 1156 3224 Fragments arranged by size: 3224 1465 1156 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2103 2297 3006 3170 1870 Size: 73 160 194 709 164 4545 Fragments arranged by size: 4545 709 194 164 160 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2972 3005 2972 Size: 33 5812 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 3166 4621 618 Size: 325 217 627 602 777 1455 1842 Fragments arranged by size: 1842 1455 777 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 2304 2655 2873 3164 1391 Size: 913 351 218 291 4072 Fragments arranged by size: 4072 913 351 291 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 928 Size: 971 198 4676 Fragments arranged by size: 4676 971 198 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3000 3737 4947 3000 Size: 737 1210 3898 Fragments arranged by size: 3898 1210 737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BfrBI ATG'CAT Cuts at: 2031 2031 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4456 136 Size: 107 122 71 631 605 2784 1525 Fragments arranged by size: 2784 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3063 5840 3063 Size: 2777 3068 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2394 2441 2823 3906 5062 1790 Size: 604 47 382 1083 1156 2573 Fragments arranged by size: 2573 1156 1083 604 382 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3576 4534 442 Size: 416 53 83 399 24 2159 958 1753 Fragments arranged by size: 2159 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 2705 4505 1239 Size: 1193 273 1800 2579 Fragments arranged by size: 2579 1800 1193 273 Bpu10I CC'TnA_GC Cuts at: 2867 2867 Size: 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2883 4508 882 Size: 617 1384 1625 2219 Fragments arranged by size: 2219 1625 1384 617 BsaAI yAC'GTr Cuts at: 493 1204 2806 3636 493 Size: 711 1602 830 2702 Fragments arranged by size: 2702 1602 830 711 BsaBI GATnn'nnATC Cuts at: 5839 5839 Size: 5845 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3652 4034 4669 275 Size: 53 83 186 936 2119 382 635 1451 Fragments arranged by size: 2119 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2503 4278 4676 4950 5097 5508 2503 Size: 1775 398 274 147 411 2840 Fragments arranged by size: 2840 1775 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 4909 Fragments arranged by size: 4909 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2384 2429 1186 Size: 1051 147 45 4602 Fragments arranged by size: 4602 1051 147 45 BseYI C'CCAG_C Cuts at: 2059 2910 5048 5544 2059 Size: 851 2138 496 2360 Fragments arranged by size: 2360 2138 851 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2441 2655 3906 3991 5062 2441 Size: 214 1251 85 1071 3224 Fragments arranged by size: 3224 1251 1071 214 85 BsmI GAATG_Cn' Cuts at: 2033 3356 3449 2033 Size: 1323 93 4429 Fragments arranged by size: 4429 1323 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2394 2441 2655 2823 2873 3164 Size: 399 514 90 47 214 168 50 291 Cuts at: 3164 3906 3991 5062 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 399 291 214 168 90 85 50 47 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 2397 3734 5464 2397 Size: 1337 1730 2778 Fragments arranged by size: 2778 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 2691 3200 844 Size: 1453 394 509 3489 Fragments arranged by size: 3489 1453 509 394 BsrBI CCG'CTC Cuts at: 1394 1823 3106 3732 1394 Size: 429 1283 626 3507 Fragments arranged by size: 3507 1283 626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2245 2667 4340 4514 65 Size: 1183 997 422 1673 174 1396 Fragments arranged by size: 1673 1396 1183 997 422 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2433 4489 4676 1700 Size: 110 623 2056 187 2869 Fragments arranged by size: 2869 2056 623 187 110 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 3811 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 2016 2028 794 Size: 1222 12 4611 Fragments arranged by size: 4611 1222 12 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3063 3175 3542 3927 3944 5396 5482 5494 Size: 1091 112 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1091 385 367 346 112 86 17 12 BstZ17I GTA'TAC Cuts at: 2737 2737 Size: 5845 Bsu36I CC'TnA_GG Cuts at: 2428 2428 Size: 5845 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 513 Size: 552 939 360 3994 Fragments arranged by size: 3994 939 552 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 3361 4155 4175 3361 Size: 794 20 5031 Fragments arranged by size: 5031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2710 4852 809 Size: 507 1394 2142 1802 Fragments arranged by size: 2142 1802 1394 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2492 2899 3292 Size: 54 1425 50 275 18 662 407 393 Cuts at: 3292 4181 5810 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 662 407 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2255 3006 3775 1353 Size: 902 751 769 3423 Fragments arranged by size: 3423 902 769 751 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2603 4432 4806 4952 387 Size: 1064 138 1014 1829 374 146 1280 Fragments arranged by size: 1829 1280 1064 1014 374 146 138 EcoICRI GAG'CTC Cuts at: 2653 2653 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2109 2432 2705 3908 4505 5292 Size: 393 391 86 323 273 1203 597 787 Cuts at: 5292 5560 1239 Size: 268 1524 Fragments arranged by size: 1524 1203 787 597 393 391 323 273 268 86 EcoNI CCTnn'n_nnAGG Cuts at: 2786 2786 Size: 5845 EcoO109I rG'GnC_Cy Cuts at: 2063 2083 2572 2761 2063 Size: 20 489 189 5147 Fragments arranged by size: 5147 489 189 20 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 2666 4351 2666 Size: 1685 4160 FspAI rTGC'GCAy Cuts at: 2666 2666 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2062 2084 2087 2094 Size: 32 135 32 483 7 22 3 7 Cuts at: 2094 2116 2188 2220 2762 2794 2818 2850 4486 Size: 22 72 32 542 32 24 32 1636 Cuts at: 4486 4518 4560 4592 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1636 542 483 135 72 42 32 32 32 32 32 32 32 24 22 22 7 7 3 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4431 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2484 4959 5143 1305 Size: 1179 2475 184 2007 Fragments arranged by size: 2475 2007 1179 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 2882 2928 3054 3803 4162 4321 Size: 648 1014 702 46 126 749 359 159 Cuts at: 4321 518 Size: 2042 Fragments arranged by size: 2042 1014 749 702 648 359 159 126 46 MspA1I CmG'CkG Cuts at: 1606 2013 2491 2917 3261 3938 5086 5331 1606 Size: 407 478 426 344 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 478 426 407 344 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2364 513 Size: 552 939 360 3994 Fragments arranged by size: 3994 939 552 360 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NsiI A_TGCA'T Cuts at: 2033 2033 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 2796 2927 3203 4748 1132 Size: 97 1567 131 276 1545 2229 Fragments arranged by size: 2229 1567 1545 276 131 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5845 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2764 3097 1108 Size: 1656 333 3856 Fragments arranged by size: 3856 1656 333 PfoI T'CCnGG_A Cuts at: 2757 2838 2757 Size: 81 5764 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2063 2083 2572 2761 2063 Size: 20 489 189 5147 Fragments arranged by size: 5147 489 189 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspXI vC'TCGA_Gb Cuts at: 2297 2297 Size: 5845 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2672 2704 2672 Size: 32 5813 PstI C_TGCA'G Cuts at: 830 2157 2864 2922 3197 830 Size: 1327 707 58 275 3478 Fragments arranged by size: 3478 1327 707 275 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2917 2917 Size: 5845 SacI G_AGCT'C Cuts at: 2655 2655 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SanDI GG'GwC_CC Cuts at: 2063 2063 Size: 5845 SapI GCTCTTCn'nnn_ Cuts at: 3006 3006 Size: 5845 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 2726 4093 5805 2726 Size: 1367 1712 2766 Fragments arranged by size: 2766 1712 1367 SexAI A'CCwGG_T Cuts at: 2658 2763 2658 Size: 105 5740 SfcI C'TryA_G Cuts at: 826 1045 2153 2860 2918 2981 3193 4328 5009 Size: 219 1108 707 58 63 212 1135 681 Cuts at: 5009 5200 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1108 707 681 219 212 191 63 58 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2297 3949 4850 5112 820 Size: 197 926 354 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 354 262 197 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 2751 3284 513 Size: 552 939 360 387 533 3074 Fragments arranged by size: 3074 939 552 533 387 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3872 3889 4042 4227 4672 325 Size: 1255 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2229 2688 Size: 274 80 33 51 1443 153 99 459 Cuts at: 2688 2724 4091 5803 96 Size: 36 1367 1712 138 Fragments arranged by size: 1712 1443 1367 459 274 153 138 99 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2574 2606 5805 5837 2574 Size: 32 3199 32 2582 Fragments arranged by size: 3199 2582 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2120 1316 Size: 804 5041 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 2297 1943 Size: 354 5491 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 3974 3974 Size: 5845 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI RsrII SacII SfiI SfoI SrfI SwaI XcmI