Restriction map for
pFN21AE1938
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiEI
BsiWI BsmFI Bsp1407I BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 55 CGCG CG^CG
AvaII 877 GGTCC G^G[AT]CC
BamHI 1409 GGATCC G^GATCC
BbrPI 856 CACGTG CAC^GTG
BbsI 469 GAAGAC GAAGAC(2/6)
BbvCI 657 CCTCAGC CCTCAGC(-5/-2)
BglI 853 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1328 AGATCT A^GATCT
BpuAI 469 GAAGAC GAAGAC(2/6)
BsaWI 1406 ACCGGA [AT]^CCGG[AT]
BseRI 704 GAGGAG GAGGAG(10/8)
BsmI 571 GCATTC GAATGC(1/-1)
BspDI 1093 ATCGAT AT^CGAT
BsrBI 63 CCGCTC CCGCTC(-3/-3)
BsrDI 864 CATTGC GCAATG(2/0)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BssHII 54 GCGCGC G^CGCGC
BssSI 635 CTCGTG CACGAG(-5/-1)
BstAPI 1122 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 55 CGCG CG^CG
BstXI 974 CCAGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
ClaI 1093 ATCGAT AT^CGAT
EcoNI 324 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 527 ATGCAT ATGCA^T
HphI 442 GGTGA GGTGA(8/7)
MspA1I 698 CAGCTG C[AC]G^C[GT]G
MvnI 55 CGCG CG^CG
NaeI 69 GCCGGC GCC^GGC
NcoI 760 CCATGG C^CATGG
NdeI 767 CATATG CA^TATG
NgoMIV 69 GCCGGC G^CCGGC
NsiI 527 ATGCAT ATGCA^T
PflMI 594 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 472 GACTC GAGTC(4/5)
PmaCI 856 CACGTG CAC^GTG
PmlI 856 CACGTG CAC^GTG
PpuMI 876 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 698 CAGCTG CAG^CTG
SacI 433 GAGCTC GAGCT^C
SphI 561 GCATGC GCATG^C
TfiI 306 GAATC G^A[AT]TC
Van91I 594 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1206 TCTAGA T^CTAGA
XcmI 200 CCACTTGCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 6 GGCGTC G[GA]^CG[CT]C
84 GACGCC
Alw44I 964 GTGCAC G^TGCAC
1064 GTGCAC
ApaI 350 GGGCCC GGGCC^C
607 GGGCCC
ApaLI 964 GTGCAC G^TGCAC
1064 GTGCAC
AvaI 374 CCCGGG C^[CT]CG[GA]G
1166 CCCGGG
BfaI 589 CTAG C^TAG
1207 CTAG
BsaAI 856 CACGTG [CT]AC^GT[GA]
1322 TACGTG
BsaHI 6 GGCGTC G[GA]^CG[CT]C
84 GACGCC
BsmBI 139 GAGACG CGTCTC(1/5)
1048 GAGACG
BsoBI 374 CCCGGG C^[CT]CG[GA]G
1166 CCCGGG
BspMI 557 ACCTGC ACCTGC(4/8)
144 GCAGGT
EcoT14I 410 CCAAGG C^C[AT][AT]GG
760 CCATGG
FauI 59 CCCGC CCCGC(4/6)
507 GCGGG
HaeII 120 GGCGCT [GA]GCGC^[CT]
402 GGCGCT
Hin1I 6 GGCGTC G[GA]^CG[CT]C
84 GACGCC
HinfI 306 GAATC G^A[ACGT]TC
472 GACTC
MaeI 589 CTAG C^TAG
1207 CTAG
MaeIII 443 GTGAC ^GT[ACGT]AC
1079 GTGAC
MboII 252 GAAGA GAAGA(8/7)
469 GAAGA
MseI 359 TTAA T^TAA
551 TTAA
NspI 561 GCATGC [GA]CATG^[CT]
1421 GCATGT
SfcI 366 CTACAG C^T[GA][CT]AG
459 CTGTAG
SmaI 374 CCCGGG CCC^GGG
1166 CCCGGG
StyI 410 CCAAGG C^C[AT][AT]GG
760 CCATGG
TaqI 681 TCGA T^CGA
1094 TCGA
Tru9I 359 TTAA T^TAA
551 TTAA
Tsp45I 443 GTGAC ^GT[GC]AC
1079 GTGAC
TthHB8I 681 TCGA T^CGA
1094 TCGA
XmaCI 374 CCCGGG C^CCGGG
1166 CCCGGG
XmaI 374 CCCGGG C^CCGGG
1166 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BalI BanII BbvI BcnI
BmyI BpmI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MaeII MboI
MflI MluNI MnlI MscI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI XhoII