(Circular) (Six-base) MAPSORT of: pFN21AE1938.seq Check: 8410 from: 1 to: 6145 pFN21AE1938 6145 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2976 3019 4208 5592 5860 1632 Size: 1344 43 1189 1384 268 1917 Fragments arranged by size: 1917 1384 1344 1189 268 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 2754 1166 Size: 1588 4557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 2705 2728 2999 3042 3374 3888 5460 2705 Size: 23 271 43 332 514 1572 3390 Fragments arranged by size: 3390 1572 514 332 271 43 23 ApaI G_GGCC'C Cuts at: 2359 2616 2359 Size: 257 5888 ApaLI G'TGCA_C Cuts at: 2969 3069 4202 5358 2969 Size: 100 1133 1156 3756 Fragments arranged by size: 3756 1156 1133 100 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2379 3171 3470 1870 Size: 73 436 792 299 4545 Fragments arranged by size: 4545 792 436 299 73 BamHI G'GATC_C Cuts at: 3414 3475 3842 3414 Size: 61 367 5717 Fragments arranged by size: 5717 367 61 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3466 4921 618 Size: 325 217 627 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2359 2442 2616 3464 1391 Size: 968 83 174 848 4072 Fragments arranged by size: 4072 968 848 174 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 928 Size: 971 582 4592 Fragments arranged by size: 4592 971 582 BbvCI CC'TCA_GC Cuts at: 2663 2663 Size: 6145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BfrBI ATG'CAT Cuts at: 2534 2534 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2864 4756 Size: 107 122 71 631 605 337 855 1892 Cuts at: 4756 136 Size: 1525 Fragments arranged by size: 1892 1525 855 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3333 6140 3333 Size: 2807 3338 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 2047 2329 2359 2588 2616 2793 2973 3073 Size: 257 282 30 229 28 177 180 100 Cuts at: 3073 3365 4206 5362 1790 Size: 292 841 1156 2573 Fragments arranged by size: 2573 1156 841 292 282 257 229 180 177 100 30 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2610 3876 4834 Size: 416 53 83 399 24 1193 1266 958 Cuts at: 4834 442 Size: 1753 Fragments arranged by size: 1753 1266 1193 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 3355 2212 Size: 1143 5002 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2351 2727 4805 1239 Size: 875 237 376 2078 2579 Fragments arranged by size: 2579 2078 875 376 237 Bpu10I CC'TnA_GC Cuts at: 2121 2663 2121 Size: 542 5603 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2863 3329 3936 493 Size: 711 1659 466 607 2702 Fragments arranged by size: 2702 1659 711 607 466 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2090 3952 4334 Size: 53 83 186 936 479 78 1862 382 Cuts at: 4334 4969 275 Size: 635 1451 Fragments arranged by size: 1862 1451 936 635 479 382 186 83 78 53 BsaWI w'CCGG_w Cuts at: 3411 4578 4976 5250 5397 5808 3411 Size: 1167 398 274 147 411 3748 Fragments arranged by size: 3748 1167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2785 2815 3237 3267 1178 Size: 30 165 30 1382 30 422 30 4056 Fragments arranged by size: 4056 1382 422 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2724 1186 Size: 1538 4607 BseYI C'CCAG_C Cuts at: 2889 3064 3364 5348 5844 2889 Size: 175 300 1984 496 3190 Fragments arranged by size: 3190 1984 496 300 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2477 2664 2935 1163 Size: 573 741 187 271 4373 Fragments arranged by size: 4373 741 573 271 187 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2388 2442 2721 2973 3073 4206 4291 5362 2388 Size: 54 279 252 100 1133 85 1071 3171 Fragments arranged by size: 3171 1133 1071 279 252 100 85 54 BsmI GAATG_Cn' Cuts at: 2576 3656 3749 2576 Size: 1080 93 4972 Fragments arranged by size: 4972 1080 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2138 3047 2138 Size: 909 5236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2329 2359 2388 2442 2588 2616 Size: 399 257 282 30 29 54 146 28 Cuts at: 2616 2721 2793 2973 3073 3365 3464 4206 4291 Size: 105 72 180 100 292 99 742 85 Cuts at: 4291 5362 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 292 282 257 180 146 105 100 99 85 72 54 30 29 28 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2571 3500 844 Size: 1296 431 929 3489 Fragments arranged by size: 3489 1296 929 431 BsrBI CCG'CTC Cuts at: 1394 1823 2070 4032 1394 Size: 429 247 1962 3507 Fragments arranged by size: 3507 1962 429 247 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 4640 4814 65 Size: 1183 1620 1772 174 1396 Fragments arranged by size: 1772 1620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 4789 4976 1700 Size: 110 264 2715 187 2869 Fragments arranged by size: 2869 2715 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 2059 1925 Size: 134 6011 BssSI C'ACGA_G Cuts at: 2640 4205 5217 2640 Size: 1565 1012 3568 Fragments arranged by size: 3568 1565 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3133 794 Size: 2339 3806 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2419 2826 3333 3414 3475 3842 4227 4244 Size: 447 407 507 81 61 367 385 17 Cuts at: 4244 5696 5782 5794 6140 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 507 447 407 385 367 346 86 81 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2765 3153 513 Size: 552 939 12 749 388 3505 Fragments arranged by size: 3505 939 749 552 388 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 2329 2827 3134 3375 3661 4455 4475 2329 Size: 498 307 241 286 794 20 3999 Fragments arranged by size: 3999 794 498 307 286 241 20 ClaI AT'CG_AT Cuts at: 1554 3099 1554 Size: 1545 4600 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3110 3145 529 Size: 35 2546 35 3529 Fragments arranged by size: 3529 2546 35 35 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 2922 2938 Size: 54 1425 50 275 18 1035 57 16 Cuts at: 2938 3003 3265 3592 4481 6110 8 Size: 65 262 327 889 1629 43 Fragments arranged by size: 1629 1425 1035 889 327 275 262 65 57 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4075 1353 Size: 2722 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2819 4732 5106 5252 387 Size: 1064 138 1230 1913 374 146 1280 Fragments arranged by size: 1913 1280 1230 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2440 2440 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2351 2727 2976 3019 4208 4805 Size: 393 482 237 376 249 43 1189 597 Cuts at: 4805 5592 5860 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1189 787 597 482 393 376 268 249 237 43 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2355 2612 2680 2882 2355 Size: 257 68 202 5618 Fragments arranged by size: 5618 257 202 68 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2129 2411 5292 1678 Size: 451 282 2881 2531 Fragments arranged by size: 2881 2531 451 282 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2535 2567 3237 3269 4786 Size: 32 135 32 963 32 670 32 1517 Cuts at: 4786 4818 4860 4892 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1517 963 670 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5259 5443 1305 Size: 3954 184 2007 Fragments arranged by size: 3954 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2867 2924 3005 10 Size: 54 1425 343 1035 57 81 3150 Fragments arranged by size: 3150 1425 1035 343 81 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 2754 2838 3213 4103 4462 4621 Size: 648 1198 390 84 375 890 359 159 Cuts at: 4621 518 Size: 2042 Fragments arranged by size: 2042 1198 890 648 390 375 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2705 3561 4238 5386 5631 1606 Size: 1099 856 677 1148 245 2120 Fragments arranged by size: 2120 1148 1099 856 677 245 NaeI GCC'GGC Cuts at: 2076 2076 Size: 6145 NcoI C'CATG_G Cuts at: 513 1065 2004 2765 513 Size: 552 939 761 3893 Fragments arranged by size: 3893 939 761 552 NdeI CA'TA_TG Cuts at: 387 2773 387 Size: 2386 3759 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NsiI A_TGCA'T Cuts at: 2536 2536 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 2570 3430 3503 5048 1132 Size: 97 1341 860 73 1545 2229 Fragments arranged by size: 2229 1545 1341 860 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 1108 Size: 1497 4648 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 2863 2863 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2882 2882 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2355 2612 2355 Size: 257 5888 PstI C_TGCA'G Cuts at: 830 3497 830 Size: 2667 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2705 2705 Size: 6145 SacI G_AGCT'C Cuts at: 2442 2442 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SfcI C'TryA_G Cuts at: 826 1045 2371 2464 3493 4628 5309 5500 826 Size: 219 1326 93 1029 1135 681 191 1471 Fragments arranged by size: 1471 1326 1135 1029 681 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2381 3173 3472 1872 Size: 509 792 299 4545 Fragments arranged by size: 4545 792 509 299 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 2570 3503 1132 Size: 1438 933 3774 Fragments arranged by size: 3774 1438 933 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2004 2415 2765 3584 513 Size: 552 939 411 350 819 3074 Fragments arranged by size: 3074 939 819 552 411 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 2669 3056 3102 4172 4189 4342 Size: 1255 855 234 387 46 1070 17 153 Cuts at: 4342 4527 4972 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1070 855 445 387 234 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3345 4391 6103 Size: 274 80 33 51 1443 1368 1046 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 1712 1443 1368 1046 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2785 2817 6105 6137 2785 Size: 32 3288 32 2793 Fragments arranged by size: 3288 2793 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3211 3481 3211 Size: 270 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2379 3171 3470 1870 Size: 509 792 299 4545 Fragments arranged by size: 4545 792 509 299 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI