(Circular) (Six-base) MAPSORT of: pFN21AE2349.seq Check: 5258 from: 1 to: 5647 pFN21AE2349 5647 bp With 182 enzymes: SgfI * May 20, 2011 16:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3457 278 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2196 2990 1937 Size: 259 794 4594 Fragments arranged by size: 4594 794 259 Acc65I G'GTAC_C Cuts at: 2810 2968 2810 Size: 158 5489 AclI AA'CG_TT Cuts at: 1563 3774 4147 1563 Size: 2211 373 3063 Fragments arranged by size: 3063 2211 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 3710 5094 5362 1632 Size: 869 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 869 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5450 AflIII A'CryG_T Cuts at: 1203 4546 5523 1203 Size: 3343 977 1327 Fragments arranged by size: 3343 1327 977 AgeI A'CCGG_T Cuts at: 4478 4478 Size: 5647 AhdI GACnn_n'nnGTC Cuts at: 4376 4376 Size: 5647 AleI CACnn'nnGTG Cuts at: 1166 2127 2273 2379 1166 Size: 961 146 106 4434 Fragments arranged by size: 4434 961 146 106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2291 1184 Size: 1107 4540 AlwNI CAG_nnn'CTG Cuts at: 2020 2625 2647 2717 3390 4962 2020 Size: 605 22 70 673 1572 2705 Fragments arranged by size: 2705 1572 673 605 70 22 ApaI G_GGCC'C Cuts at: 2034 2034 Size: 5647 ApaLI G'TGCA_C Cuts at: 3704 4860 3704 Size: 1156 4491 ApoI r'AATT_y Cuts at: 1493 2956 3182 3334 5443 1493 Size: 1463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 226 152 AseI AT'TA_AT Cuts at: 160 1032 4201 160 Size: 872 3169 1606 Fragments arranged by size: 3169 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2972 1870 Size: 73 83 946 4545 Fragments arranged by size: 4545 946 83 73 AvrII C'CTAG_G Cuts at: 2539 2539 Size: 5647 BamHI G'GATC_C Cuts at: 2977 3344 2977 Size: 367 5280 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2343 2361 2450 2684 2810 Size: 325 217 627 556 18 89 234 126 Cuts at: 2810 2968 4423 618 Size: 158 1455 1842 Fragments arranged by size: 1842 1455 627 556 325 234 217 158 126 89 18 BanII G_rGCy'C Cuts at: 1391 2012 2034 2144 2848 2966 1391 Size: 621 22 110 704 118 4072 Fragments arranged by size: 4072 704 621 118 110 22 BbeI G_GCGC'C Cuts at: 2347 2347 Size: 5647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4676 BbvCI CC'TCA_GC Cuts at: 2595 2595 Size: 5647 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3838 3872 1903 Size: 34 1901 34 3678 Fragments arranged by size: 3678 1901 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 4749 3539 Size: 1210 4437 BclI T'GATC_A Cuts at: 1548 1548 Size: 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2029 4258 136 Size: 107 122 71 631 605 357 2229 1525 Fragments arranged by size: 2229 1525 631 605 357 122 107 71 BglII A'GATC_T Cuts at: 5642 5642 Size: 5647 BlpI GC'TnA_GC Cuts at: 2618 3064 2618 Size: 446 5201 Bme1580I G_kGCm'C Cuts at: 1790 2034 2134 3708 4864 1790 Size: 244 100 1574 1156 2573 Fragments arranged by size: 2573 1574 1156 244 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5647 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2885 3378 4336 Size: 416 53 83 399 24 1468 493 958 Cuts at: 4336 442 Size: 1753 Fragments arranged by size: 1753 1468 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5647 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2510 2562 2811 2862 4307 1239 Size: 917 354 52 249 51 1445 2579 Fragments arranged by size: 2579 1445 917 354 249 52 51 Bpu10I CC'TnA_GC Cuts at: 2595 2595 Size: 5647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3772 4637 4935 3772 Size: 865 298 4484 Fragments arranged by size: 4484 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2629 4310 882 Size: 617 1130 1681 2219 Fragments arranged by size: 2219 1681 1130 617 BsaAI yAC'GTr Cuts at: 493 1204 3438 493 Size: 711 2234 2702 Fragments arranged by size: 2702 2234 711 BsaBI GATnn'nnATC Cuts at: 5641 5641 Size: 5647 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2293 2344 3454 3836 Size: 53 83 186 936 760 51 1110 382 Cuts at: 3836 4471 275 Size: 635 1451 Fragments arranged by size: 1451 1110 936 760 635 382 186 83 53 51 BsaWI w'CCGG_w Cuts at: 4080 4478 4752 4899 5310 4080 Size: 398 274 147 411 4417 Fragments arranged by size: 4417 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2161 2189 2191 2219 1178 Size: 30 165 30 758 28 2 28 4606 Fragments arranged by size: 4606 758 165 30 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5647 BseYI C'CCAG_C Cuts at: 2085 2425 2789 4850 5346 2085 Size: 340 364 2061 496 2386 Fragments arranged by size: 2386 2061 496 364 340 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 1163 Size: 573 1006 4068 Fragments arranged by size: 4068 1006 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3097 3858 4886 1540 Size: 275 186 1096 761 1028 2301 Fragments arranged by size: 2301 1096 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2270 2443 3708 3793 4864 2270 Size: 173 1265 85 1071 3053 Fragments arranged by size: 3053 1265 1071 173 85 BsmI GAATG_Cn' Cuts at: 3158 3251 3158 Size: 93 5554 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2034 2134 2144 2270 2443 2848 Size: 399 222 22 100 10 126 173 405 Cuts at: 2848 2966 3708 3793 4864 1391 Size: 118 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 222 173 126 118 100 85 22 10 BspEI T'CCGG_A Cuts at: 5310 5310 Size: 5647 BspHI T'CATG_A Cuts at: 3536 5266 3536 Size: 1730 3917 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 3002 844 Size: 1487 671 3489 Fragments arranged by size: 3489 1487 671 BsrBI CCG'CTC Cuts at: 1394 1823 3534 1394 Size: 429 1711 3507 Fragments arranged by size: 3507 1711 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4142 4316 65 Size: 1183 2894 174 1396 Fragments arranged by size: 2894 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4291 4478 1700 Size: 110 2481 187 2869 Fragments arranged by size: 2869 2481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5647 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5647 BssSI C'ACGA_G Cuts at: 3707 4719 3707 Size: 1012 4635 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 794 Size: 1226 4421 BstBI TT'CG_AA Cuts at: 4451 4451 Size: 5647 BstEII G'GTnAC_C Cuts at: 1184 2381 1184 Size: 1197 4450 BstXI CCAn_nnnn'nTGG Cuts at: 2358 2443 2358 Size: 85 5562 BstYI r'GATC_y Cuts at: 1972 2818 2977 3344 3729 3746 5198 5284 5296 Size: 846 159 367 385 17 1452 86 12 Cuts at: 5296 5642 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 846 385 367 346 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4908 Fragments arranged by size: 4908 683 56 BtsI GCAGTG_nn' Cuts at: 2472 2754 3163 3957 3977 2472 Size: 282 409 794 20 4142 Fragments arranged by size: 4142 794 409 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5647 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5612 DraI TTT'AAA Cuts at: 2951 2951 Size: 5647 DraIII CAC_nnn'GTG Cuts at: 2189 2189 Size: 5647 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4654 809 Size: 507 3338 1802 Fragments arranged by size: 3338 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2766 2886 Size: 54 1425 50 275 18 486 450 120 Cuts at: 2886 2931 3094 3983 5612 8 Size: 45 163 889 1629 43 Fragments arranged by size: 1629 1425 889 486 450 275 163 120 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3094 1537 Size: 275 1282 4090 Fragments arranged by size: 4090 1282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 3577 1353 Size: 1169 1055 3423 Fragments arranged by size: 3423 1169 1055 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4234 4608 4754 387 Size: 1064 138 2645 374 146 1280 Fragments arranged by size: 2645 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2501 2510 2562 2811 2862 3710 Size: 393 524 345 9 52 249 51 848 Cuts at: 3710 4307 5094 5362 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 848 787 597 524 393 345 268 249 52 51 9 EcoNI CCTnn'n_nnAGG Cuts at: 2719 2719 Size: 5647 EcoO109I rG'GnC_Cy Cuts at: 2017 2030 2485 2644 2836 2017 Size: 13 455 159 192 4828 Fragments arranged by size: 4828 455 192 159 13 EcoRI G'AATT_C Cuts at: 2956 3334 2956 Size: 378 5269 FspI TGC'GCA Cuts at: 4153 4153 Size: 5647 HaeII r_GCGC'y Cuts at: 1678 2347 4794 1678 Size: 669 2447 2531 Fragments arranged by size: 2531 2447 669 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2245 2277 4288 Size: 32 135 32 617 32 24 32 2011 Cuts at: 4288 4320 4362 4394 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2011 617 135 42 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2991 3237 669 Size: 988 281 1053 246 3079 Fragments arranged by size: 3079 1053 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5647 HpaI GTT'AAC Cuts at: 3237 3237 Size: 5647 KasI G'GCGC_C Cuts at: 2343 2343 Size: 5647 KpnI G_GTAC'C Cuts at: 2814 2972 2814 Size: 158 5489 MfeI C'AATT_G Cuts at: 3246 3246 Size: 5647 MluI A'CGCG_T Cuts at: 5523 5523 Size: 5647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4761 4945 1305 Size: 3456 184 2007 Fragments arranged by size: 3456 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 2768 2888 2933 10 Size: 54 1425 343 486 450 120 45 2724 Fragments arranged by size: 2724 1425 486 450 343 120 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2127 2273 2379 3605 3964 4123 518 Size: 648 961 146 106 1226 359 159 2042 Fragments arranged by size: 2042 1226 961 648 359 159 146 106 MspA1I CmG'CkG Cuts at: 1606 2273 3063 3740 4888 5133 1606 Size: 667 790 677 1148 245 2120 Fragments arranged by size: 2120 1148 790 677 667 245 NarI GG'CG_CC Cuts at: 2344 2344 Size: 5647 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4156 Fragments arranged by size: 4156 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5647 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5647 NotI GC'GGCC_GC Cuts at: 3094 3094 Size: 5647 NspI r_CATG'y Cuts at: 1132 1229 3005 4550 1132 Size: 97 1776 1545 2229 Fragments arranged by size: 2229 1776 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5647 PciI A'CATG_T Cuts at: 4546 4546 Size: 5647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5647 PmeI GTTT'AAAC Cuts at: 2951 2951 Size: 5647 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 5229 5261 3716 Size: 32 1481 32 4102 Fragments arranged by size: 4102 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2836 2836 Size: 5647 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5647 PsiI TTA'TAA Cuts at: 3217 3217 Size: 5647 PspOMI G'GGCC_C Cuts at: 2030 2030 Size: 5647 PstI C_TGCA'G Cuts at: 830 2237 2245 2999 830 Size: 1407 8 754 3478 Fragments arranged by size: 3478 1407 754 8 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5647 PvuII CAG'CTG Cuts at: 2273 2273 Size: 5647 SalI G'TCGA_C Cuts at: 1936 2989 1936 Size: 1053 4594 SapI GCTCTTCn'nnn_ Cuts at: 2522 2522 Size: 5647 SbfI CC_TGCA'GG Cuts at: 2999 2999 Size: 5647 ScaI AGT'ACT Cuts at: 3895 5607 3895 Size: 1712 3935 SfcI C'TryA_G Cuts at: 826 1045 2233 2241 2533 2995 4130 4811 5002 Size: 219 1188 8 292 462 1135 681 191 Cuts at: 5002 826 Size: 1471 Fragments arranged by size: 1471 1188 1135 681 462 292 219 191 8 SfoI GGC'GCC Cuts at: 2345 2345 Size: 5647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5647 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5647 SmaI CCC'GGG Cuts at: 1872 2028 2974 1872 Size: 156 946 4545 Fragments arranged by size: 4545 946 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 3751 4652 4914 820 Size: 197 926 1808 901 262 1553 Fragments arranged by size: 1808 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3438 493 Size: 2945 2702 SpeI A'CTAG_T Cuts at: 152 2783 152 Size: 2631 3016 SphI G_CATG'C Cuts at: 1132 3005 1132 Size: 1873 3774 SspI AAT'ATT Cuts at: 5 52 3571 5 Size: 47 3519 2081 Fragments arranged by size: 3519 2081 47 StuI AGG'CCT Cuts at: 1517 2231 2677 1517 Size: 714 446 4487 Fragments arranged by size: 4487 714 446 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2522 2539 2794 2814 2898 Size: 552 939 87 431 17 255 20 84 Cuts at: 2898 3086 513 Size: 188 3074 Fragments arranged by size: 3074 939 552 431 255 188 87 84 20 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3674 3691 3844 4029 4474 325 Size: 1255 1067 1027 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1067 1027 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3893 5605 96 Size: 274 80 33 51 1443 1916 1712 138 Fragments arranged by size: 1916 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2161 2193 5607 5639 2161 Size: 32 3414 32 2169 Fragments arranged by size: 3414 2169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5647 XbaI T'CTAG_A Cuts at: 2983 2983 Size: 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2761 2896 2761 Size: 135 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5647 XmaI C'CCGG_G Cuts at: 1870 2026 2972 1870 Size: 156 946 4545 Fragments arranged by size: 4545 946 156 XmnI GAAnn'nnTTC Cuts at: 3776 3776 Size: 5647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3455 276 Size: 53 83 186 936 1921 2468 Fragments arranged by size: 2468 1921 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI