(Circular) (Six-base) MAPSORT of: pFN21AE3426.seq Check: 9403 from: 1 to: 6484 pFN21AE3426 6484 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3670 3757 3670 Size: 87 6397 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 4294 278 Size: 53 83 186 936 561 2197 2468 Fragments arranged by size: 2468 2197 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 2310 3805 2310 Size: 1495 4989 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 3236 3513 3515 1166 Size: 2070 277 2 4135 Fragments arranged by size: 4135 2070 277 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 3674 3706 2103 Size: 32 1539 32 4881 Fragments arranged by size: 4881 1539 32 32 AlwNI CAG_nnn'CTG Cuts at: 4227 5799 4227 Size: 1572 4912 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2519 3793 4019 4171 6280 1493 Size: 1026 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 1026 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3647 3809 1870 Size: 73 1704 162 4545 Fragments arranged by size: 4545 1704 162 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2293 2310 2319 2368 2473 Size: 325 217 627 506 17 9 49 105 Cuts at: 2473 2744 2803 3097 3805 5260 618 Size: 271 59 294 708 1455 1842 Fragments arranged by size: 1842 1455 708 627 506 325 294 271 217 105 59 49 17 9 BanII G_rGCy'C Cuts at: 1391 3071 3803 1391 Size: 1680 732 4072 Fragments arranged by size: 4072 1680 732 BbeI G_GCGC'C Cuts at: 2323 2748 2807 2323 Size: 425 59 6000 Fragments arranged by size: 6000 425 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2974 2998 3265 928 Size: 971 1075 24 267 4147 Fragments arranged by size: 4147 1075 971 267 24 BbvCI CC'TCA_GC Cuts at: 3557 3600 3557 Size: 43 6441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3742 5095 136 Size: 107 122 71 631 605 2070 1353 1525 Fragments arranged by size: 2070 1525 1353 631 605 122 107 71 BglII A'GATC_T Cuts at: 2115 6479 2115 Size: 4364 2120 BlpI GC'TnA_GC Cuts at: 2060 2763 3901 2060 Size: 703 1138 4643 Fragments arranged by size: 4643 1138 703 Bme1580I G_kGCm'C Cuts at: 1790 2371 2394 2484 3102 4545 5701 1790 Size: 581 23 90 618 1443 1156 2573 Fragments arranged by size: 2573 1443 1156 618 581 90 23 BmgBI CAC'GTC Cuts at: 1318 2493 1318 Size: 1175 5309 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2137 4215 5173 Size: 416 53 83 399 24 720 2078 958 Cuts at: 5173 442 Size: 1753 Fragments arranged by size: 2078 1753 958 720 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3745 3745 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3659 5144 1239 Size: 2420 1485 2579 Fragments arranged by size: 2579 2420 1485 Bpu10I CC'TnA_GC Cuts at: 2136 3557 3600 3709 3738 2136 Size: 1421 43 109 29 4882 Fragments arranged by size: 4882 1421 109 43 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 3085 4609 5474 5772 2144 Size: 941 1524 865 298 2856 Fragments arranged by size: 2856 1524 941 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3170 3304 3635 3644 5147 882 Size: 617 1671 134 331 9 1503 2219 Fragments arranged by size: 2219 1671 1503 617 331 134 9 BsaAI yAC'GTr Cuts at: 493 1204 4275 493 Size: 711 3071 2702 Fragments arranged by size: 3071 2702 711 BsaBI GATnn'nnATC Cuts at: 2212 6478 2212 Size: 4266 2218 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2320 2603 2745 Size: 53 83 186 936 561 226 283 142 Cuts at: 2745 2804 3258 4291 4673 5308 275 Size: 59 454 1033 382 635 1451 Fragments arranged by size: 1451 1033 936 635 561 454 382 283 226 186 142 83 59 53 BsaWI w'CCGG_w Cuts at: 2398 3213 4917 5315 5589 5736 6147 2398 Size: 815 1704 398 274 147 411 2735 Fragments arranged by size: 2735 1704 815 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 3053 3083 1178 Size: 30 165 30 1497 30 123 30 4579 Fragments arranged by size: 4579 1497 165 123 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 2792 2795 3737 1186 Size: 1108 498 3 942 3933 Fragments arranged by size: 3933 1108 942 498 3 BseYI C'CCAG_C Cuts at: 2362 2463 2988 3057 3631 5687 6183 2362 Size: 101 525 69 574 2056 496 2663 Fragments arranged by size: 2663 2056 574 525 496 101 69 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 2633 2638 2906 1163 Size: 573 375 522 5 268 4741 Fragments arranged by size: 4741 573 522 375 268 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3934 4695 5723 1540 Size: 275 186 1189 744 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 744 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2137 2625 2736 3071 3123 3159 3243 4545 Size: 76 488 111 335 52 36 84 1302 Cuts at: 4545 4630 5701 2061 Size: 85 1071 2844 Fragments arranged by size: 2844 1302 1071 488 335 111 85 84 76 52 36 BsiWI C'GTAC_G Cuts at: 2070 2070 Size: 6484 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2137 2371 2394 2484 2625 2736 Size: 399 271 76 234 23 90 141 111 Cuts at: 2736 3071 3102 3123 3159 3243 3803 4545 4630 Size: 335 31 21 36 84 560 742 85 Cuts at: 4630 5701 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 560 399 335 271 234 141 111 90 85 84 76 36 31 23 21 BspEI T'CCGG_A Cuts at: 2398 6147 2398 Size: 3749 2735 BspHI T'CATG_A Cuts at: 2097 4373 6103 2097 Size: 2276 1730 2478 Fragments arranged by size: 2478 2276 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2928 3670 3757 3839 844 Size: 1454 630 742 87 82 3489 Fragments arranged by size: 3489 1454 742 630 87 82 BsrBI CCG'CTC Cuts at: 1394 1823 3742 4371 1394 Size: 429 1919 629 3507 Fragments arranged by size: 3507 1919 629 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 4979 5153 65 Size: 1183 1213 2518 174 1396 Fragments arranged by size: 2518 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2831 3185 3189 5128 5315 1700 Size: 110 1021 354 4 1939 187 2869 Fragments arranged by size: 2869 1939 1021 354 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 2701 2788 1925 Size: 776 87 5621 Fragments arranged by size: 5621 776 87 BssSI C'ACGA_G Cuts at: 2538 4544 5556 2538 Size: 2006 1012 3466 Fragments arranged by size: 3466 2006 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2191 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2115 3814 4181 4566 4583 6035 6121 6133 Size: 143 1699 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1699 1452 385 367 346 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2580 3498 3666 513 Size: 552 939 576 918 168 3331 Fragments arranged by size: 3331 939 918 576 552 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2779 487 Size: 683 56 1553 4192 Fragments arranged by size: 4192 1553 683 56 BtsI GCAGTG_nn' Cuts at: 2650 3576 4000 4794 4814 2650 Size: 926 424 794 20 4320 Fragments arranged by size: 4320 926 794 424 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 2375 3054 2375 Size: 679 5805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2218 5491 809 Size: 507 902 3273 1802 Fragments arranged by size: 3273 1802 902 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2357 2880 3042 Size: 54 1425 50 275 18 527 523 162 Cuts at: 3042 3187 3279 3592 3610 3743 3931 4820 6449 Size: 145 92 313 18 133 188 889 1629 Cuts at: 6449 8 Size: 43 Fragments arranged by size: 1629 1425 889 527 523 313 275 188 162 145 133 92 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 3931 1537 Size: 275 1375 744 4090 Fragments arranged by size: 4090 1375 744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3140 3347 3759 4414 1353 Size: 1787 207 412 655 3423 Fragments arranged by size: 3423 1787 655 412 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3069 3069 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3659 4547 5144 5931 6199 1239 Size: 393 2027 888 597 787 268 1524 Fragments arranged by size: 2027 1524 888 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2638 2774 3719 3758 2638 Size: 136 945 39 5364 Fragments arranged by size: 5364 945 136 39 EcoO109I rG'GnC_Cy Cuts at: 2167 3627 2167 Size: 1460 5024 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2039 2323 2748 2770 2807 5631 1678 Size: 361 284 425 22 37 2824 2531 Fragments arranged by size: 2824 2531 425 361 284 37 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 3053 3085 3351 Size: 32 135 32 665 32 784 32 266 Cuts at: 3351 3383 5125 5157 5199 5231 1373 Size: 32 1742 32 42 32 2626 Fragments arranged by size: 2626 1742 784 665 266 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 2319 2744 2803 2319 Size: 425 59 6000 Fragments arranged by size: 6000 425 59 KpnI G_GTAC'C Cuts at: 2314 3809 2314 Size: 1495 4989 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 3044 3281 3612 10 Size: 54 1425 343 1050 162 237 331 2882 Fragments arranged by size: 2882 1425 1050 343 331 237 162 54 MslI CAynn'nnrTG Cuts at: 518 1166 2672 2936 3095 3236 3513 3515 4442 Size: 648 1506 264 159 141 277 2 927 Cuts at: 4442 4801 4960 518 Size: 359 159 2042 Fragments arranged by size: 2042 1506 927 648 359 277 264 159 159 141 2 MspA1I CmG'CkG Cuts at: 1606 2362 2463 2828 3002 3432 3471 3604 3900 Size: 756 101 365 174 430 39 133 296 Cuts at: 3900 4577 5725 5970 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 756 677 430 365 296 245 174 133 101 39 NaeI GCC'GGC Cuts at: 2833 3187 2833 Size: 354 6130 NarI GG'CG_CC Cuts at: 2320 2745 2804 2320 Size: 425 59 6000 Fragments arranged by size: 6000 425 59 NcoI C'CATG_G Cuts at: 513 1065 2004 3498 3666 513 Size: 552 939 1494 168 3331 Fragments arranged by size: 3331 1494 939 552 168 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 2831 3185 2831 Size: 354 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2677 3842 5387 1132 Size: 97 1448 1165 1545 2229 Fragments arranged by size: 2229 1545 1448 1165 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6484 PfoI T'CCnGG_A Cuts at: 3071 3071 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 3627 2167 Size: 1460 5024 PshAI GACnn'nnGTC Cuts at: 1613 3524 1613 Size: 1911 4573 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PstI C_TGCA'G Cuts at: 830 2175 2849 3836 830 Size: 1345 674 987 3478 Fragments arranged by size: 3478 1345 987 674 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2463 3604 2463 Size: 1141 5343 SacI G_AGCT'C Cuts at: 3071 3071 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3627 3627 Size: 6484 SapI GCTCTTCn'nnn_ Cuts at: 3347 3347 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SexAI A'CCwGG_T Cuts at: 2371 2371 Size: 6484 SfcI C'TryA_G Cuts at: 826 1045 2171 2503 2845 3484 3758 3832 4967 Size: 219 1126 332 342 639 274 74 1135 Cuts at: 4967 5648 5839 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1126 681 639 342 332 274 219 191 74 SfiI GGCCn_nnn'nGGCC Cuts at: 3742 3742 Size: 6484 SfoI GGC'GCC Cuts at: 2321 2746 2805 2321 Size: 425 59 6000 Fragments arranged by size: 6000 425 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 3064 3647 4588 5489 5751 Size: 197 926 216 905 583 941 901 262 Cuts at: 5751 820 Size: 1553 Fragments arranged by size: 1553 941 926 905 901 583 262 216 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 3714 3728 1517 Size: 2197 14 4273 Fragments arranged by size: 4273 2197 14 StyI C'CwwG_G Cuts at: 513 1065 2004 3498 3666 3678 3923 513 Size: 552 939 1494 168 12 245 3074 Fragments arranged by size: 3074 1494 939 552 245 168 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 4511 4528 4681 4866 5311 325 Size: 1255 731 2200 17 153 185 445 1498 Fragments arranged by size: 2200 1498 1255 731 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6444 6476 6444 Size: 32 6452 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 3647 1943 Size: 1704 4780 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 4292 276 Size: 53 83 186 936 561 2197 2468 Fragments arranged by size: 2468 2197 936 561 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SrfI SwaI XcmI