(Circular) (Six-base) MAPSORT of: pFN21AE6789.seq Check: 3967 from: 1 to: 6445 pFN21AE6789 6445 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 2730 3766 2730 Size: 1036 5409 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2684 3569 4508 5892 6160 1632 Size: 1052 885 939 1384 268 1917 Fragments arranged by size: 1917 1384 1052 939 885 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 3356 3605 1166 Size: 2190 249 4006 Fragments arranged by size: 4006 2190 249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AlwNI CAG_nnn'CTG Cuts at: 2182 4188 5760 2182 Size: 2006 1572 2867 Fragments arranged by size: 2867 2006 1572 ApaI G_GGCC'C Cuts at: 2762 3046 2762 Size: 284 6161 ApaLI G'TGCA_C Cuts at: 2209 2604 4502 5658 2209 Size: 395 1898 1156 2996 Fragments arranged by size: 2996 1898 1156 395 ApoI r'AATT_y Cuts at: 1493 3754 3980 4132 6241 1493 Size: 2261 226 152 2109 1697 Fragments arranged by size: 2261 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 3323 3770 1870 Size: 73 1380 447 4545 Fragments arranged by size: 4545 1380 447 73 AvrII C'CTAG_G Cuts at: 2854 2854 Size: 6445 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2730 3471 3491 3766 5221 Size: 325 217 627 943 741 20 275 1455 Cuts at: 5221 618 Size: 1842 Fragments arranged by size: 1842 1455 943 741 627 325 275 217 20 BanII G_rGCy'C Cuts at: 1391 2330 2762 3046 3096 3764 1391 Size: 939 432 284 50 668 4072 Fragments arranged by size: 4072 939 668 432 284 50 BbeI G_GCGC'C Cuts at: 3495 3495 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2672 2960 928 Size: 971 773 288 4413 Fragments arranged by size: 4413 971 773 288 BbvCI CC'TCA_GC Cuts at: 2117 2274 2117 Size: 157 6288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2155 2189 4636 4670 1903 Size: 34 50 34 134 34 2447 34 3678 Fragments arranged by size: 3678 2447 134 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3287 4337 5547 3287 Size: 1050 1210 4185 Fragments arranged by size: 4185 1210 1050 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BfrBI ATG'CAT Cuts at: 3037 3037 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2567 3500 5056 Size: 107 122 71 631 605 895 933 1556 Cuts at: 5056 136 Size: 1525 Fragments arranged by size: 1556 1525 933 895 631 605 122 107 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 2630 3264 3862 2630 Size: 634 598 5213 Fragments arranged by size: 5213 634 598 Bme1580I G_kGCm'C Cuts at: 1790 2213 2608 2659 2762 3046 4506 5662 1790 Size: 423 395 51 103 284 1460 1156 2573 Fragments arranged by size: 2573 1460 1156 423 395 284 103 51 BmgBI CAC'GTC Cuts at: 1318 2458 2503 1318 Size: 1140 45 5260 Fragments arranged by size: 5260 1140 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4176 5134 442 Size: 416 53 83 399 24 2759 958 1753 Fragments arranged by size: 2759 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2993 3152 5105 1239 Size: 881 873 159 1953 2579 Fragments arranged by size: 2579 1953 881 873 159 Bpu10I CC'TnA_GC Cuts at: 2117 2274 3219 2117 Size: 157 945 5343 Fragments arranged by size: 5343 945 157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4570 5435 5733 4570 Size: 865 298 5282 Fragments arranged by size: 5282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5108 882 Size: 617 3609 2219 Fragments arranged by size: 3609 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3607 4236 493 Size: 711 2403 629 2702 Fragments arranged by size: 2702 2403 711 629 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3479 3492 4252 4634 Size: 53 83 186 936 1946 13 760 382 Cuts at: 4634 5269 275 Size: 635 1451 Fragments arranged by size: 1946 1451 936 760 635 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2312 2342 3455 3485 3551 Size: 30 165 30 909 30 1113 30 66 Cuts at: 3551 3581 1178 Size: 30 4042 Fragments arranged by size: 4042 1113 909 165 66 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2120 2291 2876 1186 Size: 931 3 171 585 4755 Fragments arranged by size: 4755 931 585 171 3 BseYI C'CCAG_C Cuts at: 2143 2592 3045 5648 6144 2143 Size: 449 453 2603 496 2444 Fragments arranged by size: 2603 2444 496 453 449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2768 1163 Size: 573 1032 4840 Fragments arranged by size: 4840 1032 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2213 2608 3096 4506 4591 5662 2213 Size: 395 488 1410 85 1071 2996 Fragments arranged by size: 2996 1410 1071 488 395 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 BsmBI CGTCTCn'nnnn_ Cuts at: 2785 2785 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2330 2608 2659 2762 3046 3096 Size: 399 423 117 278 51 103 284 50 Cuts at: 3096 3764 4506 4591 5662 1391 Size: 668 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 423 399 284 278 117 103 85 51 50 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 2509 2814 4334 6064 2509 Size: 305 1520 1730 2890 Fragments arranged by size: 2890 1730 1520 305 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 3800 844 Size: 1682 1274 3489 Fragments arranged by size: 3489 1682 1274 BsrBI CCG'CTC Cuts at: 1394 1823 3223 4332 1394 Size: 429 1400 1109 3507 Fragments arranged by size: 3507 1400 1109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2794 3019 4940 5114 65 Size: 1183 1546 225 1921 174 1396 Fragments arranged by size: 1921 1546 1396 1183 225 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2440 2676 3405 5089 5276 1700 Size: 110 630 236 729 1684 187 2869 Fragments arranged by size: 2869 1684 729 630 236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 2564 4505 5517 2564 Size: 1941 1012 3492 Fragments arranged by size: 3492 1941 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6445 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 3052 1184 Size: 1868 4577 BstXI CCAn_nnnn'nTGG Cuts at: 3673 3673 Size: 6445 BstYI r'GATC_y Cuts at: 1972 2178 2793 3525 3702 3775 4142 4527 4544 Size: 206 615 732 177 73 367 385 17 Cuts at: 4544 5996 6082 6094 6440 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 732 615 385 367 346 206 177 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2293 2367 3073 3299 3416 3530 513 Size: 552 1228 74 706 226 117 114 3428 Fragments arranged by size: 3428 1228 706 552 226 117 114 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2452 487 Size: 683 56 1226 4480 Fragments arranged by size: 4480 1226 683 56 BtsI GCAGTG_nn' Cuts at: 2171 3961 4755 4775 2171 Size: 1790 794 20 3841 Fragments arranged by size: 3841 1790 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 2206 2206 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5452 809 Size: 507 4136 1802 Fragments arranged by size: 4136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3144 3407 3892 Size: 54 1425 50 275 18 1314 263 485 Cuts at: 3892 4781 6410 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1314 889 485 275 263 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2582 3068 3101 3338 4375 1353 Size: 1181 48 486 33 237 1037 3423 Fragments arranged by size: 3423 1181 1037 486 237 48 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3199 5032 5406 5552 387 Size: 1064 138 1610 1833 374 146 1280 Fragments arranged by size: 1833 1610 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3094 3094 Size: 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2684 2993 3152 3569 4508 5105 Size: 393 488 564 309 159 417 939 597 Cuts at: 5105 5892 6160 1239 Size: 787 268 1524 Fragments arranged by size: 1524 939 787 597 564 488 417 393 309 268 159 EcoNI CCTnn'n_nnAGG Cuts at: 2186 2858 2186 Size: 672 5773 EcoO109I rG'GnC_Cy Cuts at: 2758 3042 3295 3713 2758 Size: 284 253 418 5490 Fragments arranged by size: 5490 418 284 253 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FseI GG_CCGG'CC Cuts at: 3409 3409 Size: 6445 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 2338 3495 5592 1678 Size: 660 1157 2097 2531 Fragments arranged by size: 2531 2097 1157 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3353 3385 5086 5118 5160 Size: 32 135 32 1781 32 1701 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 1781 1701 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 3491 3491 Size: 6445 KpnI G_GTAC'C Cuts at: 2734 3770 2734 Size: 1036 5409 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 5559 5743 1305 Size: 984 3270 184 2007 Fragments arranged by size: 3270 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3146 10 Size: 54 1425 343 1314 3309 Fragments arranged by size: 3309 1425 1314 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 3356 3605 4403 4762 4921 518 Size: 648 1035 1155 249 798 359 159 2042 Fragments arranged by size: 2042 1155 1035 798 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2054 2662 3049 3687 3861 4538 5686 5931 Size: 448 608 387 638 174 677 1148 245 Cuts at: 5931 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 638 608 448 387 245 174 NaeI GCC'GGC Cuts at: 2678 3407 2678 Size: 729 5716 NarI GG'CG_CC Cuts at: 3492 3492 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 2293 3530 513 Size: 552 1228 1237 3428 Fragments arranged by size: 3428 1237 1228 552 NdeI CA'TA_TG Cuts at: 387 3103 387 Size: 2716 3729 NgoMIV G'CCGG_C Cuts at: 2676 3405 2676 Size: 729 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NsiI A_TGCA'T Cuts at: 3039 3039 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 2072 2090 3037 3803 5348 1132 Size: 97 843 18 947 766 1545 2229 Fragments arranged by size: 2229 1545 947 843 766 97 18 PasI CC'CwG_GG Cuts at: 1348 2671 2755 3228 1348 Size: 1323 84 473 4565 Fragments arranged by size: 4565 1323 473 84 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6445 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3295 3713 3295 Size: 418 6027 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 2758 3042 2758 Size: 284 6161 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3527 3559 3527 Size: 32 6413 PstI C_TGCA'G Cuts at: 830 2178 3585 3630 3797 830 Size: 1348 1407 45 167 3478 Fragments arranged by size: 3478 1407 1348 167 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2662 3049 2662 Size: 387 6058 SacI G_AGCT'C Cuts at: 3096 3096 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SanDI GG'GwC_CC Cuts at: 3713 3713 Size: 6445 SapI GCTCTTCn'nnn_ Cuts at: 2582 3101 3338 2582 Size: 519 237 5689 Fragments arranged by size: 5689 519 237 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 2390 4693 6405 2390 Size: 2303 1712 2430 Fragments arranged by size: 2430 2303 1712 SexAI A'CCwGG_T Cuts at: 2726 3672 2726 Size: 946 5499 SfcI C'TryA_G Cuts at: 826 1045 2174 3581 3626 3793 4928 5609 5800 Size: 219 1129 1407 45 167 1135 681 191 Cuts at: 5800 826 Size: 1471 Fragments arranged by size: 1471 1407 1135 1129 681 219 191 167 45 SfoI GGC'GCC Cuts at: 3493 3493 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3772 1872 Size: 1900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4549 5450 5712 820 Size: 197 926 2606 901 262 1553 Fragments arranged by size: 2606 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 2090 3037 3803 1132 Size: 958 947 766 3774 Fragments arranged by size: 3774 958 947 766 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6445 StyI C'CwwG_G Cuts at: 513 1065 2293 2854 3354 3530 3884 513 Size: 552 1228 561 500 176 354 3074 Fragments arranged by size: 3074 1228 561 552 500 354 176 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3263 4472 4489 4642 4827 5272 325 Size: 1255 1683 1209 17 153 185 445 1498 Fragments arranged by size: 1683 1498 1255 1209 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 2865 4691 Size: 274 80 33 51 1443 411 477 1826 Cuts at: 4691 6403 96 Size: 1712 138 Fragments arranged by size: 1826 1712 1443 477 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 3455 3487 6405 6437 2163 Size: 32 1260 32 2918 32 2171 Fragments arranged by size: 2918 2171 1260 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2965 1316 Size: 1649 4796 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3391 3391 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3770 1870 Size: 1900 4545 XmnI GAAnn'nnTTC Cuts at: 4574 4574 Size: 6445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI NruI PacI PfoI PmlI PspXI RsrII SacII SfiI SrfI SwaI