(Linear) (Six-base) MAPSORT of: KIEE4169.seq Check: 4211 from: 1 to: 1863 KIEE4169.orf With 182 enzymes: SgfI * March 22, 2012 17:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1801 1863 Size: 1801 62 AccI GT'mk_AC Cuts at: 0 1391 1863 Size: 1391 472 Acc65I G'GTAC_C Cuts at: 0 1669 1863 Size: 1669 194 AfeI AGC'GCT Cuts at: 0 1279 1863 Size: 1279 584 AhdI GACnn_n'nnGTC Cuts at: 0 1311 1863 Size: 1311 552 AleI CACnn'nnGTG Cuts at: 0 484 1863 Size: 484 1379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 961 1863 Size: 961 902 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 565 597 1709 1741 1863 Size: 565 32 1112 32 122 Fragments arranged by size: 1112 565 122 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 255 1863 Size: 255 1608 AvaI C'yCGr_G Cuts at: 0 742 1863 Size: 742 1121 BamHI G'GATC_C Cuts at: 0 911 1863 Size: 911 952 BanI G'GyrC_C Cuts at: 0 1479 1611 1669 1813 1863 Size: 1479 132 58 144 50 Fragments arranged by size: 1479 144 132 58 50 BanII G_rGCy'C Cuts at: 0 68 530 1739 1863 Size: 68 462 1209 124 Fragments arranged by size: 1209 462 124 68 BbsI GAAGACnn'nnnn_ Cuts at: 0 399 651 1074 1118 1863 Size: 399 252 423 44 745 Fragments arranged by size: 745 423 399 252 44 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 78 112 1863 Size: 78 34 1751 Fragments arranged by size: 1751 78 34 BciVI GTATCCnnnnn_n' Cuts at: 0 910 1863 Size: 910 953 BclI T'GATC_A Cuts at: 0 965 1863 Size: 965 898 BglI GCCn_nnn'nGGC Cuts at: 0 844 1214 1863 Size: 844 370 649 Fragments arranged by size: 844 649 370 BlpI GC'TnA_GC Cuts at: 0 632 1863 Size: 632 1231 Bme1580I G_kGCm'C Cuts at: 0 738 859 1614 1863 Size: 738 121 755 249 Fragments arranged by size: 755 738 249 121 BmgBI CAC'GTC Cuts at: 0 240 1053 1863 Size: 240 813 810 Fragments arranged by size: 813 810 240 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1246 1659 1863 Size: 1246 413 204 Fragments arranged by size: 1246 413 204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 528 1112 1723 1736 1770 1863 Size: 241 287 584 611 13 34 93 Fragments arranged by size: 611 584 287 241 93 34 13 BsaAI yAC'GTr Cuts at: 0 1245 1350 1395 1863 Size: 1245 105 45 468 Fragments arranged by size: 1245 468 105 45 BsaHI Gr'CG_yC Cuts at: 0 617 1863 Size: 617 1246 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 19 49 214 244 565 595 1696 1709 Size: 19 30 165 30 321 30 1101 13 Cuts at: 1709 1726 1739 1863 Size: 17 13 124 Fragments arranged by size: 1101 321 165 124 30 30 30 19 17 13 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 40 238 586 892 901 988 1777 1780 Size: 40 198 348 306 9 87 789 3 Cuts at: 1780 1863 Size: 83 Fragments arranged by size: 789 348 306 198 87 83 40 9 3 BseYI C'CCAG_C Cuts at: 0 6 737 1176 1546 1863 Size: 6 731 439 370 317 Fragments arranged by size: 731 439 370 317 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 241 322 781 1863 Size: 199 42 81 459 1082 Fragments arranged by size: 1082 459 199 81 42 BsiEI CG_ry'CG Cuts at: 0 521 1863 Size: 521 1342 BsiHKAI G_wGCw'C Cuts at: 0 530 1373 1739 1863 Size: 530 843 366 124 Fragments arranged by size: 843 530 366 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 418 445 1699 1863 Size: 418 27 1254 164 Fragments arranged by size: 1254 418 164 27 Bsp1286I G_dGCh'C Cuts at: 0 68 530 738 859 1373 1614 1739 1863 Size: 68 462 208 121 514 241 125 124 Fragments arranged by size: 514 462 241 208 125 124 121 68 BspHI T'CATG_A Cuts at: 0 962 1863 Size: 962 901 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1801 1863 Size: 1801 62 BsrBI CCG'CTC Cuts at: 0 1499 1863 Size: 1499 364 BsrGI T'GTAC_A Cuts at: 0 266 1586 1863 Size: 266 1320 277 Fragments arranged by size: 1320 277 266 BssHII G'CGCG_C Cuts at: 0 178 201 434 490 642 644 690 1863 Size: 178 23 233 56 152 2 46 1173 Fragments arranged by size: 1173 233 178 152 56 46 23 2 BssSI C'ACGA_G Cuts at: 0 727 781 1863 Size: 727 54 1082 Fragments arranged by size: 1082 727 54 BstAPI GCAn_nnn'nTGC Cuts at: 0 1813 1863 Size: 1813 50 BstEII G'GTnAC_C Cuts at: 0 1062 1551 1863 Size: 1062 489 312 Fragments arranged by size: 1062 489 312 BstXI CCAn_nnnn'nTGG Cuts at: 0 399 1245 1863 Size: 399 846 618 Fragments arranged by size: 846 618 399 BstYI r'GATC_y Cuts at: 0 335 911 951 1863 Size: 335 576 40 912 Fragments arranged by size: 912 576 335 40 BtgI C'CryG_G Cuts at: 0 84 317 446 1863 Size: 84 233 129 1417 Fragments arranged by size: 1417 233 129 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 124 516 1863 Size: 124 392 1347 Fragments arranged by size: 1347 392 124 BtsI GCAGTG_nn' Cuts at: 0 584 1419 1863 Size: 584 835 444 Fragments arranged by size: 835 584 444 DraIII CAC_nnn'GTG Cuts at: 0 1368 1863 Size: 1368 495 EaeI y'GGCC_r Cuts at: 0 152 401 518 1359 1527 1863 Size: 152 249 117 841 168 336 Fragments arranged by size: 841 336 249 168 152 117 EagI C'GGCC_G Cuts at: 0 518 1863 Size: 518 1345 EarI CTCTTCn'nnn_ Cuts at: 0 1105 1863 Size: 1105 758 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 37 1863 Size: 37 1826 EcoICRI GAG'CTC Cuts at: 0 528 1737 1863 Size: 528 1209 126 Fragments arranged by size: 1209 528 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 528 1112 1723 1736 1770 1863 Size: 241 287 584 611 13 34 93 Fragments arranged by size: 611 584 287 241 93 34 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 223 1863 Size: 223 1640 EcoO109I rG'GnC_Cy Cuts at: 0 1038 1125 1863 Size: 1038 87 738 Fragments arranged by size: 1038 738 87 FspI TGC'GCA Cuts at: 0 754 1863 Size: 754 1109 HaeII r_GCGC'y Cuts at: 0 7 381 639 720 983 1281 1863 Size: 7 374 258 81 263 298 582 Fragments arranged by size: 582 374 298 263 258 81 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 214 246 1297 1329 1696 1728 1863 Size: 214 32 1051 32 367 32 135 Fragments arranged by size: 1051 367 214 135 32 32 32 KpnI G_GTAC'C Cuts at: 0 1673 1863 Size: 1673 190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1055 1863 Size: 416 639 808 Fragments arranged by size: 808 639 416 MscI TGG'CCA Cuts at: 0 1361 1863 Size: 1361 502 MslI CAynn'nnrTG Cuts at: 0 358 484 961 1243 1320 1537 1863 Size: 358 126 477 282 77 217 326 Fragments arranged by size: 477 358 326 282 217 126 77 MspA1I CmG'CkG Cuts at: 0 33 319 1191 1205 1341 1546 1863 Size: 33 286 872 14 136 205 317 Fragments arranged by size: 872 317 286 205 136 33 14 NcoI C'CATG_G Cuts at: 0 84 1863 Size: 84 1779 NspI r_CATG'y Cuts at: 0 78 942 1863 Size: 78 864 921 Fragments arranged by size: 921 864 78 PflMI CCAn_nnn'nTGG Cuts at: 0 144 1863 Size: 144 1719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 112 144 565 597 1709 1741 1863 Size: 112 32 421 32 1112 32 122 Fragments arranged by size: 1112 421 122 112 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1038 1125 1863 Size: 1038 87 738 Fragments arranged by size: 1038 738 87 PstI C_TGCA'G Cuts at: 0 163 374 950 1151 1863 Size: 163 211 576 201 712 Fragments arranged by size: 712 576 211 201 163 PvuII CAG'CTG Cuts at: 0 1546 1863 Size: 1546 317 SacI G_AGCT'C Cuts at: 0 530 1739 1863 Size: 530 1209 124 Fragments arranged by size: 1209 530 124 SacII CC_GC'GG Cuts at: 0 320 1863 Size: 320 1543 SbfI CC_TGCA'GG Cuts at: 0 950 1863 Size: 950 913 SexAI A'CCwGG_T Cuts at: 0 1034 1076 1863 Size: 1034 42 787 Fragments arranged by size: 1034 787 42 SfcI C'TryA_G Cuts at: 0 159 370 946 1147 1863 Size: 159 211 576 201 716 Fragments arranged by size: 716 576 211 201 159 SnaBI TAC'GTA Cuts at: 0 1395 1863 Size: 1395 468 StuI AGG'CCT Cuts at: 0 1097 1624 1863 Size: 1097 527 239 Fragments arranged by size: 1097 527 239 StyI C'CwwG_G Cuts at: 0 84 839 1067 1863 Size: 84 755 228 796 Fragments arranged by size: 796 755 228 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1281 1497 1863 Size: 1281 216 366 Fragments arranged by size: 1281 366 216 TatI w'GTAC_w Cuts at: 0 266 1586 1863 Size: 266 1320 277 Fragments arranged by size: 1320 277 266 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 314 346 1156 1188 1863 Size: 314 32 810 32 675 Fragments arranged by size: 810 675 314 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1291 1387 1710 1863 Size: 1291 96 323 153 Fragments arranged by size: 1291 323 153 96 Enzymes that do cut: AarI AccI Acc65I AfeI AhdI AleI AlfI AloI AlwNI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI NspI PflMI PpiI PpuMI PstI PvuII SacI SacII SbfI SexAI SfcI SnaBI StuI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AatII AclI AcuI AflII AflIII AgeI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI BsmI BspEI BsrDI BsrFI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI ZraI