(Circular) (Six-base) MAPSORT of: pFN21AE1921.seq Check: 3857 from: 1 to: 6076 pFN21AE1921 6076 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2225 3234 2225 Size: 1009 5067 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3886 278 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3419 1937 Size: 1482 4594 Acc65I G'GTAC_C Cuts at: 2797 3397 2797 Size: 600 5476 AclI AA'CG_TT Cuts at: 1563 4203 4576 1563 Size: 2640 373 3063 Fragments arranged by size: 3063 2640 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2948 3123 4139 5523 5791 1632 Size: 1316 175 1016 1384 268 1917 Fragments arranged by size: 1917 1384 1316 1016 268 175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5879 AflIII A'CryG_T Cuts at: 1203 4975 5952 1203 Size: 3772 977 1327 Fragments arranged by size: 3772 1327 977 AgeI A'CCGG_T Cuts at: 4907 4907 Size: 6076 AhdI GACnn_n'nnGTC Cuts at: 4805 4805 Size: 6076 AleI CACnn'nnGTG Cuts at: 1166 2816 2884 1166 Size: 1650 68 4358 Fragments arranged by size: 4358 1650 68 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6076 AlwNI CAG_nnn'CTG Cuts at: 2155 2971 3819 5391 2155 Size: 816 848 1572 2840 Fragments arranged by size: 2840 1572 848 816 ApaI G_GGCC'C Cuts at: 2071 2903 2071 Size: 832 5244 ApaLI G'TGCA_C Cuts at: 4133 5289 4133 Size: 1156 4920 ApoI r'AATT_y Cuts at: 1493 3385 3611 3763 5872 1493 Size: 1892 226 152 2109 1697 Fragments arranged by size: 2109 1892 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4630 160 Size: 872 3598 1606 Fragments arranged by size: 3598 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 AvaI C'yCGr_G Cuts at: 1870 1943 2235 3401 1870 Size: 73 292 1166 4545 Fragments arranged by size: 4545 1166 292 73 BamHI G'GATC_C Cuts at: 2835 3406 3773 2835 Size: 571 367 5138 Fragments arranged by size: 5138 571 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2797 3222 3261 3284 Size: 325 217 627 267 743 425 39 23 Cuts at: 3284 3332 3397 4852 618 Size: 48 65 1455 1842 Fragments arranged by size: 1842 1455 743 627 425 325 267 217 65 48 39 23 BanII G_rGCy'C Cuts at: 1391 2071 2639 2903 3126 3395 1391 Size: 680 568 264 223 269 4072 Fragments arranged by size: 4072 680 568 269 264 223 BbeI G_GCGC'C Cuts at: 2058 3288 2058 Size: 1230 4846 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2438 2572 928 Size: 971 539 134 4432 Fragments arranged by size: 4432 971 539 134 BbvCI CC'TCA_GC Cuts at: 3200 3200 Size: 6076 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4267 4301 1903 Size: 34 50 34 2246 34 3678 Fragments arranged by size: 3678 2246 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 5178 3968 Size: 1210 4866 BclI T'GATC_A Cuts at: 1548 1548 Size: 6076 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4687 136 Size: 107 122 71 631 605 3015 1525 Fragments arranged by size: 3015 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6071 6071 Size: 6076 BlpI GC'TnA_GC Cuts at: 3493 3493 Size: 6076 Bme1580I G_kGCm'C Cuts at: 1790 2071 2903 3337 4137 5293 1790 Size: 281 832 434 800 1156 2573 Fragments arranged by size: 2573 1156 832 800 434 281 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6076 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3807 4765 442 Size: 416 53 83 399 24 2390 958 1753 Fragments arranged by size: 2390 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3023 1056 Size: 1967 4109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2192 2363 3331 4736 1239 Size: 938 15 171 968 1405 2579 Fragments arranged by size: 2579 1405 968 938 171 15 Bpu10I CC'TnA_GC Cuts at: 2495 3200 2495 Size: 705 5371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2998 4201 5066 5364 2998 Size: 1203 865 298 3710 Fragments arranged by size: 3710 1203 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4739 882 Size: 617 3240 2219 Fragments arranged by size: 3240 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3291 3867 493 Size: 711 2087 576 2702 Fragments arranged by size: 2702 2087 711 576 BsaBI GATnn'nnATC Cuts at: 6070 6070 Size: 6076 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3234 3285 3883 Size: 53 83 186 936 522 1179 51 598 Cuts at: 3883 4265 4900 275 Size: 382 635 1451 Fragments arranged by size: 1451 1179 936 635 598 522 382 186 83 53 51 BsaWI w'CCGG_w Cuts at: 3085 4509 4907 5181 5328 5739 3085 Size: 1424 398 274 147 411 3422 Fragments arranged by size: 3422 1424 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2612 2642 1178 Size: 30 165 30 1209 30 4612 Fragments arranged by size: 4612 1209 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2174 2275 2866 3334 1186 Size: 937 51 101 591 468 3928 Fragments arranged by size: 3928 937 591 468 101 51 BseYI C'CCAG_C Cuts at: 2166 2790 2801 2859 5279 5775 2166 Size: 624 11 58 2420 496 2467 Fragments arranged by size: 2467 2420 624 496 58 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2159 2302 2503 3209 3313 1163 Size: 573 408 15 143 201 706 104 3926 Fragments arranged by size: 3926 706 573 408 201 143 104 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3526 4287 5315 1540 Size: 275 186 1525 761 1028 2301 Fragments arranged by size: 2301 1525 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2630 3126 4137 4222 5293 2630 Size: 496 1011 85 1071 3413 Fragments arranged by size: 3413 1071 1011 496 85 BsmI GAATG_Cn' Cuts at: 3587 3680 3587 Size: 93 5983 BsmBI CGTCTCn'nnnn_ Cuts at: 2746 2746 Size: 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2630 2639 2903 3126 3337 3395 Size: 399 281 559 9 264 223 211 58 Cuts at: 3395 4137 4222 5293 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 281 264 223 211 85 58 9 BspEI T'CCGG_A Cuts at: 3085 5739 3085 Size: 2654 3422 BspHI T'CATG_A Cuts at: 3965 5695 3965 Size: 1730 4346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 3234 3292 3431 844 Size: 1381 1009 58 139 3489 Fragments arranged by size: 3489 1381 1009 139 58 BsrBI CCG'CTC Cuts at: 1394 1823 2758 3342 3963 1394 Size: 429 935 584 621 3507 Fragments arranged by size: 3507 935 621 584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2308 2361 4571 4745 65 Size: 1183 1060 53 2210 174 1396 Fragments arranged by size: 2210 1396 1183 1060 174 53 BsrFI r'CCGG_y Cuts at: 1700 1810 2077 2435 3258 4720 4907 1700 Size: 110 267 358 823 1462 187 2869 Fragments arranged by size: 2869 1462 823 358 267 187 110 BsrGI T'GTAC_A Cuts at: 96 2724 96 Size: 2628 3448 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6076 BssSI C'ACGA_G Cuts at: 4136 5148 4136 Size: 1012 5064 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6076 BstBI TT'CG_AA Cuts at: 4880 4880 Size: 6076 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6076 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 6076 BstYI r'GATC_y Cuts at: 1972 2835 3406 3773 4158 4175 5627 5713 5725 Size: 863 571 367 385 17 1452 86 12 Cuts at: 5725 6071 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 863 571 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2564 Size: 6076 BtgI C'CryG_G Cuts at: 513 1065 2658 2814 513 Size: 552 1593 156 3775 Fragments arranged by size: 3775 1593 552 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2812 487 Size: 683 56 1586 3751 Fragments arranged by size: 3751 1586 683 56 BtsI GCAGTG_nn' Cuts at: 2156 2515 3592 4386 4406 2156 Size: 359 1077 794 20 3826 Fragments arranged by size: 3826 1077 794 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6076 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6041 DraI TTT'AAA Cuts at: 3380 3380 Size: 6076 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5083 809 Size: 507 3767 1802 Fragments arranged by size: 3767 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2086 2094 2222 Size: 54 1425 50 275 18 256 8 128 Cuts at: 2222 3523 4412 6041 8 Size: 1301 889 1629 43 Fragments arranged by size: 1629 1425 1301 889 275 256 128 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 3523 1537 Size: 275 1711 4090 Fragments arranged by size: 4090 1711 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2552 2606 2987 4006 1353 Size: 932 267 54 381 1019 3423 Fragments arranged by size: 3423 1019 932 381 267 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3209 3220 4663 5037 5183 387 Size: 1064 138 1620 11 1443 374 146 1280 Fragments arranged by size: 1620 1443 1280 1064 374 146 138 11 EcoICRI GAG'CTC Cuts at: 3124 3124 Size: 6076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2192 2363 2948 3123 3331 4139 Size: 393 545 15 171 585 175 208 808 Cuts at: 4139 4736 5523 5791 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 808 787 597 585 545 393 268 208 175 171 15 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2159 Size: 6076 EcoO109I rG'GnC_Cy Cuts at: 2068 2713 2899 2900 2068 Size: 645 186 1 5244 Fragments arranged by size: 5244 645 186 1 EcoRI G'AATT_C Cuts at: 3385 3763 3385 Size: 378 5698 EcoRV GAT'ATC Cuts at: 2986 2986 Size: 6076 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3133 3133 Size: 6076 FspI TGC'GCA Cuts at: 4582 4582 Size: 6076 HaeII r_GCGC'y Cuts at: 1678 2058 3220 3288 5223 1678 Size: 380 1162 68 1935 2531 Fragments arranged by size: 2531 1935 1162 380 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 4717 4749 4791 Size: 32 135 32 1040 32 2073 32 42 Cuts at: 4791 4823 1373 Size: 32 2626 Fragments arranged by size: 2626 2073 1040 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3666 669 Size: 988 281 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6076 HpaI GTT'AAC Cuts at: 3666 3666 Size: 6076 KasI G'GCGC_C Cuts at: 2054 3284 2054 Size: 1230 4846 KpnI G_GTAC'C Cuts at: 2801 3401 2801 Size: 600 5476 MfeI C'AATT_G Cuts at: 3675 3675 Size: 6076 MluI A'CGCG_T Cuts at: 5952 5952 Size: 6076 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 5190 5374 1305 Size: 1293 2592 184 2007 Fragments arranged by size: 2592 2007 1293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2224 10 Size: 54 1425 343 392 3862 Fragments arranged by size: 3862 1425 392 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 2816 2884 4034 4393 4552 518 Size: 648 1371 279 68 1150 359 159 2042 Fragments arranged by size: 2042 1371 1150 648 359 279 159 68 MspA1I CmG'CkG Cuts at: 1606 2023 2034 2170 2316 2576 2610 3492 4169 Size: 417 11 136 146 260 34 882 677 Cuts at: 4169 5317 5562 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 882 677 417 260 245 146 136 34 11 NaeI GCC'GGC Cuts at: 2079 2079 Size: 6076 NarI GG'CG_CC Cuts at: 2055 3285 2055 Size: 1230 4846 NcoI C'CATG_G Cuts at: 513 1065 2658 2814 513 Size: 552 1593 156 3775 Fragments arranged by size: 3775 1593 552 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 6076 NgoMIV G'CCGG_C Cuts at: 2077 2077 Size: 6076 NheI G'CTAG_C Cuts at: 1052 3019 1052 Size: 1967 4109 NotI GC'GGCC_GC Cuts at: 3523 3523 Size: 6076 NspI r_CATG'y Cuts at: 1132 1229 2542 3434 4979 1132 Size: 97 1313 892 1545 2229 Fragments arranged by size: 2229 1545 1313 892 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6076 PciI A'CATG_T Cuts at: 4975 4975 Size: 6076 PflMI CCAn_nnn'nTGG Cuts at: 1108 2820 1108 Size: 1712 4364 PmeI GTTT'AAAC Cuts at: 3380 3380 Size: 6076 PmlI CAC'GTG Cuts at: 3291 3291 Size: 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4145 4177 5658 5690 4145 Size: 32 1481 32 4531 Fragments arranged by size: 4531 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3003 3320 1613 Size: 1390 317 4369 Fragments arranged by size: 4369 1390 317 PsiI TTA'TAA Cuts at: 3646 3646 Size: 6076 PspOMI G'GGCC_C Cuts at: 2067 2899 2067 Size: 832 5244 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3239 3271 3239 Size: 32 6044 PstI C_TGCA'G Cuts at: 830 2106 2195 3231 3428 830 Size: 1276 89 1036 197 3478 Fragments arranged by size: 3478 1276 1036 197 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6076 PvuII CAG'CTG Cuts at: 2170 2316 2170 Size: 146 5930 SacI G_AGCT'C Cuts at: 3126 3126 Size: 6076 SalI G'TCGA_C Cuts at: 1936 3418 1936 Size: 1482 4594 SapI GCTCTTCn'nnn_ Cuts at: 2285 2285 Size: 6076 SbfI CC_TGCA'GG Cuts at: 3231 3428 3231 Size: 197 5879 ScaI AGT'ACT Cuts at: 2765 4324 6036 2765 Size: 1559 1712 2805 Fragments arranged by size: 2805 1712 1559 SfcI C'TryA_G Cuts at: 826 1045 2102 2191 3227 3424 4559 5240 5431 Size: 219 1057 89 1036 197 1135 681 191 Cuts at: 5431 826 Size: 1471 Fragments arranged by size: 1471 1135 1057 1036 681 219 197 191 89 SfoI GGC'GCC Cuts at: 2056 3286 2056 Size: 1230 4846 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6076 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6076 SmaI CCC'GGG Cuts at: 1872 3403 1872 Size: 1531 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3013 4180 5081 5343 820 Size: 197 926 1070 1167 901 262 1553 Fragments arranged by size: 1553 1167 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3867 493 Size: 3374 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6076 SphI G_CATG'C Cuts at: 1132 3434 1132 Size: 2302 3774 SspI AAT'ATT Cuts at: 5 52 2675 4000 5 Size: 47 2623 1325 2081 Fragments arranged by size: 2623 2081 1325 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6076 StyI C'CwwG_G Cuts at: 513 1065 2071 2658 2814 3515 513 Size: 552 1006 587 156 701 3074 Fragments arranged by size: 3074 1006 701 587 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2844 4103 4120 4273 4458 4903 325 Size: 1255 1264 1259 17 153 185 445 1498 Fragments arranged by size: 1498 1264 1259 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 2763 3058 Size: 274 80 33 51 1443 747 39 295 Cuts at: 3058 4322 6034 96 Size: 1264 1712 138 Fragments arranged by size: 1712 1443 1264 747 295 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2840 2872 6036 6068 2840 Size: 32 3164 32 2848 Fragments arranged by size: 3164 2848 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6076 XbaI T'CTAG_A Cuts at: 3412 3412 Size: 6076 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6076 XmaI C'CCGG_G Cuts at: 1870 3401 1870 Size: 1531 4545 XmnI GAAnn'nnTTC Cuts at: 2765 4205 2765 Size: 1440 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3884 276 Size: 53 83 186 936 2350 2468 Fragments arranged by size: 2468 2350 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI