################################## # NO CUT ENZYMES ( 213 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BciVI BclI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstNI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI MwoI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcsI 196 AAATTT [GA]^AATT[CT] AfaI 212 GTAC GT^AC AflII 322 CTTAAG C^TTAAG AlwNI 74 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 196 AAATTT [GA]^AATT[CT] Asp700I 180 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 30 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 69 CCCGG CC^[GC]GG BfrI 322 CTTAAG C^TTAAG BsiYI 229 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 229 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 76 GGGAC GGGAC(10/14) BsrDI 147 CATTGC GCAATG(2/0) BsrI 282 CCAGT ACTGG(1/-1) BssKI 69 CCCGG ^CC[ACGT]GG BstEII 259 GGTGACC G^GT[ACGT]ACC BstF5I 226 CATCC GGATG(2/0) BstPI 259 GGTGACC G^GT[ACGT]ACC EcoO65I 259 GGTGACC G^GT[ACGT]ACC FokI 226 CATCC GGATG(9/13) HapII 70 CCGG C^CGG HindIII 182 AAGCTT A^AGCTT HpaII 70 CCGG C^CGG MseI 323 TTAA T^TAA MspI 70 CCGG C^CGG NciI 69 CCCGG CC^[GC]GG NlaIII 331 CATG CATG^ RsaI 212 GTAC GT^AC ScrFI 69 CCCGG CC^[ACGT]GG SfaNI 43 GATGC GCATC(5/9) SmlI 322 CTTAAG C^T[CT][GA]AG TaqI 29 TCGA T^CGA Tru9I 323 TTAA T^TAA Tsp509I 197 AATT ^AATT TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 29 TCGA T^CGA XmnI 180 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### DdeI 50 CTAAG C^T[ACGT]AG 80 CTGAG HinfI 101 GAATC G^A[ACGT]TC 335 GATTC HphI 55 GGTGA GGTGA(8/7) 259 GGTGA MaeIII 56 GTGAC ^GT[ACGT]AC 260 GTGAC TfiI 101 GAATC G^A[AT]TC 335 GATTC Tsp45I 56 GTGAC ^GT[GC]AC 260 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### AluI MnlI