################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 446 CGCG CG^CG AcsI 657 AAATTT [GA]^AATT[CT] AfaI 217 GTAC GT^AC AflIII 198 ACGTGT A^C[GA][CT]GT AlwI 144 GGATC GGATC(4/5) AocI 492 CCTGAGG CC^T[ACGT]AGG ApoI 657 AAATTT [GA]^AATT[CT] AvaI 830 CCCGAG C^[CT]CG[GA]G AvaII 113 GGACC G^G[AT]CC BanI 518 GGCACC G^G[CT][GA]CC BclI 80 TGATCA T^GATCA BcnI 522 CCGGG CC^[GC]GG BfaI 194 CTAG C^TAG BsaAI 803 TACGTG [CT]AC^GT[GA] BsaWI 75 TCCGGT [AT]^CCGG[AT] BseRI 495 GAGGAG GAGGAG(10/8) BsmAI 47 GAGAC GTCTC(1/5) BsoBI 830 CCCGAG C^[CT]CG[GA]G BspHI 621 TCATGA T^CATGA BsrDI 859 GCAATG GCAATG(2/0) BsrI 696 ACTGG ACTGG(1/-1) BstUI 446 CGCG CG^CG BstYI 144 GGATCT [GA]^GATC[CT] Bsu36I 492 CCTGAGG CC^T[ACGT]AGG Eco81I 492 CCTGAGG CC^T[ACGT]AGG EcoT14I 816 CCAAGG C^C[AT][AT]GG FbaI 80 TGATCA T^GATCA HgaI 444 GACGC GACGC(5/10) MaeI 194 CTAG C^TAG MflI 144 GGATCT [GA]^GATC[CT] MseI 152 TTAA T^TAA MspA1I 663 CAGCTG C[AC]G^C[GT]G MvnI 446 CGCG CG^CG MwoI 856 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 522 CCGGG CC^[GC]GG NdeI 730 CATATG CA^TATG PflMI 135 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 112 GGGACCT [GA]G^G[AT]CC[CT] PvuII 663 CAGCTG CAG^CTG RcaI 621 TCATGA T^CATGA RsaI 217 GTAC GT^AC SexAI 626 ACCAGGT A^CC[AT]GGT StyI 816 CCAAGG C^C[AT][AT]GG TaqI 558 TCGA T^CGA Tru9I 152 TTAA T^TAA Tsp509I 658 AATT ^AATT TthHB8I 558 TCGA T^CGA Van91I 135 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 193 TCTAGA T^CTAGA XhoII 144 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 664 AGCT AG^CT 737 AGCT BalI 765 TGGCCA TGG^CCA 890 TGGCCA BanII 393 GGGCTC G[GA]GC[CT]^C 833 GAGCCC BpmI 172 CTCCAG CTGGAG(16/14) 370 CTGGAG BspMI 115 ACCTGC ACCTGC(4/8) 708 ACCTGC CfoI 447 GCGC GCG^C 565 GCGC DraII 112 GGGACCT [GA]G^G[ACGT]CC[CT] 524 GGGGCCT EaeI 765 TGGCCA [CT]^GGCC[GA] 890 TGGCCA EarI 529 CTCTTC CTCTTC(1/4) 880 GAAGAG EcoO109I 112 GGGACCT [GA]G^G[ACGT]CC[CT] 524 GGGGCCT HapII 76 CCGG C^CGG 522 CCGG HhaI 447 GCGC GCG^C 565 GCGC HinP1I 447 GCGC G^CGC 565 GCGC HpaII 76 CCGG C^CGG 522 CCGG HphI 78 GGTGA GGTGA(8/7) 424 TCACC Ksp632I 529 CTCTTC CTCTTC(1/4) 880 GAAGAG MluNI 765 TGGCCA TGG^CCA 890 TGGCCA MscI 765 TGGCCA TGG^CCA 890 TGGCCA MspI 76 CCGG C^CGG 522 CCGG PleI 243 GAGTC GAGTC(4/5) 373 GAGTC Tsp45I 423 GTCAC ^GT[GC]AC 775 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeII MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI TspRI