(Circular) (Six-base) MAPSORT of: pFN21AE3281.seq Check: 7620 from: 1 to: 6841 pFN21AE3281 6841 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2851 2851 Size: 6841 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4651 278 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4184 1937 Size: 2247 4594 Acc65I G'GTAC_C Cuts at: 4162 4162 Size: 6841 AclI AA'CG_TT Cuts at: 1563 4968 5341 1563 Size: 3405 373 3063 Fragments arranged by size: 3405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3328 3512 3906 4904 6288 6556 1632 Size: 1696 184 394 998 1384 268 1917 Fragments arranged by size: 1917 1696 1384 998 394 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6644 AflIII A'CryG_T Cuts at: 1203 5740 6717 1203 Size: 4537 977 1327 Fragments arranged by size: 4537 1327 977 AgeI A'CCGG_T Cuts at: 2884 5672 2884 Size: 2788 4053 AhdI GACnn_n'nnGTC Cuts at: 5570 5570 Size: 6841 AleI CACnn'nnGTG Cuts at: 1166 4067 1166 Size: 2901 3940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3303 1184 Size: 2119 4722 AlwNI CAG_nnn'CTG Cuts at: 2246 4584 6156 2246 Size: 2338 1572 2931 Fragments arranged by size: 2931 2338 1572 ApaLI G'TGCA_C Cuts at: 4898 6054 4898 Size: 1156 5685 ApoI r'AATT_y Cuts at: 1493 2260 2290 2650 2985 3040 3469 4150 4376 Size: 767 30 360 335 55 429 681 226 Cuts at: 4376 4528 6637 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 767 681 429 360 335 226 152 55 30 AseI AT'TA_AT Cuts at: 160 1032 3263 3522 5395 160 Size: 872 2231 259 1873 1606 Fragments arranged by size: 2231 1873 1606 872 259 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 2217 2664 4166 1870 Size: 73 274 447 1502 4545 Fragments arranged by size: 4545 1502 447 274 73 AvrII C'CTAG_G Cuts at: 3134 3134 Size: 6841 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2661 2694 2661 Size: 33 6808 BamHI G'GATC_C Cuts at: 2437 4171 4538 2437 Size: 1734 367 4740 Fragments arranged by size: 4740 1734 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2103 2660 4162 5617 618 Size: 325 217 627 316 557 1502 1455 1842 Fragments arranged by size: 1842 1502 1455 627 557 325 316 217 BanII G_rGCy'C Cuts at: 1391 2190 2218 2386 2635 2847 3134 3835 4160 Size: 799 28 168 249 212 287 701 325 Cuts at: 4160 1391 Size: 4072 Fragments arranged by size: 4072 799 701 325 287 249 212 168 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 3129 928 Size: 971 137 1093 4640 Fragments arranged by size: 4640 1093 971 137 BbvCI CC'TCA_GC Cuts at: 2307 2307 Size: 6841 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5032 5066 1903 Size: 34 50 34 3011 34 3678 Fragments arranged by size: 3678 3011 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2874 3469 4733 5943 2874 Size: 595 1264 1210 3772 Fragments arranged by size: 3772 1264 1210 595 BclI T'GATC_A Cuts at: 1548 2011 3052 1548 Size: 463 1041 5337 Fragments arranged by size: 5337 1041 463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 5452 136 Size: 107 122 71 631 605 526 3254 1525 Fragments arranged by size: 3254 1525 631 605 526 122 107 71 BglII A'GATC_T Cuts at: 2086 2328 3402 3967 6836 2086 Size: 242 1074 565 2869 2091 Fragments arranged by size: 2869 2091 1074 565 242 BlpI GC'TnA_GC Cuts at: 3922 4258 3922 Size: 336 6505 Bme1580I G_kGCm'C Cuts at: 1790 2164 2665 4902 6058 1790 Size: 374 501 2237 1156 2573 Fragments arranged by size: 2573 2237 1156 501 374 BmgBI CAC'GTC Cuts at: 1318 4065 1318 Size: 2747 4094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4572 5530 442 Size: 416 53 83 399 24 3155 958 1753 Fragments arranged by size: 3155 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2950 5501 1239 Size: 1711 2551 2579 Fragments arranged by size: 2579 2551 1711 Bpu10I CC'TnA_GC Cuts at: 2307 2307 Size: 6841 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3148 3352 4966 5831 6129 3148 Size: 204 1614 865 298 3860 Fragments arranged by size: 3860 1614 865 298 204 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2966 5504 882 Size: 617 1467 2538 2219 Fragments arranged by size: 2538 2219 1467 617 BsaAI yAC'GTr Cuts at: 493 1204 3431 4632 493 Size: 711 2227 1201 2702 Fragments arranged by size: 2702 2227 1201 711 BsaBI GATnn'nnATC Cuts at: 6835 6835 Size: 6841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4648 5030 5665 275 Size: 53 83 186 936 3115 382 635 1451 Fragments arranged by size: 3115 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2884 5274 5672 5946 6093 6504 2884 Size: 2390 398 274 147 411 3221 Fragments arranged by size: 3221 2390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2874 2904 1178 Size: 30 165 30 1471 30 5115 Fragments arranged by size: 5115 1471 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2882 2915 3049 1186 Size: 1696 33 134 4978 Fragments arranged by size: 4978 1696 134 33 BseYI C'CCAG_C Cuts at: 3667 6044 6540 3667 Size: 2377 496 3968 Fragments arranged by size: 3968 2377 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2859 4118 1163 Size: 573 1123 1259 3886 Fragments arranged by size: 3886 1259 1123 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4291 5052 6080 1540 Size: 275 186 2290 761 1028 2301 Fragments arranged by size: 2301 2290 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2218 3204 4902 4987 6058 2218 Size: 986 1698 85 1071 3001 Fragments arranged by size: 3001 1698 1071 986 85 BsmI GAATG_Cn' Cuts at: 3292 4352 4445 3292 Size: 1060 93 5688 Fragments arranged by size: 5688 1060 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2164 2190 2218 2386 2635 2665 2847 Size: 399 374 26 28 168 249 30 182 Cuts at: 2847 3134 3204 3835 4160 4902 4987 6058 1391 Size: 287 70 631 325 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 631 399 374 325 287 249 182 168 85 70 30 28 26 BspEI T'CCGG_A Cuts at: 6504 6504 Size: 6841 BspHI T'CATG_A Cuts at: 4730 6460 4730 Size: 1730 5111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2851 4196 844 Size: 2007 1345 3489 Fragments arranged by size: 3489 2007 1345 BsrBI CCG'CTC Cuts at: 1394 1823 4728 1394 Size: 429 2905 3507 Fragments arranged by size: 3507 2905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3648 5336 5510 65 Size: 1183 2400 1688 174 1396 Fragments arranged by size: 2400 1688 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2884 5485 5672 1700 Size: 110 1074 2601 187 2869 Fragments arranged by size: 2869 2601 1074 187 110 BsrGI T'GTAC_A Cuts at: 96 3804 96 Size: 3708 3133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 4901 5913 4901 Size: 1012 5829 BstAPI GCAn_nnn'nTGC Cuts at: 794 3089 794 Size: 2295 4546 BstBI TT'CG_AA Cuts at: 5645 5645 Size: 6841 BstEII G'GTnAC_C Cuts at: 1184 2617 1184 Size: 1433 5408 BstYI r'GATC_y Cuts at: 1972 2086 2328 2437 3402 3967 4171 4538 4923 Size: 114 242 109 965 565 204 367 385 Cuts at: 4923 4940 6392 6478 6490 6836 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 965 565 385 367 346 242 204 114 109 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6102 Fragments arranged by size: 6102 683 56 BtsI GCAGTG_nn' Cuts at: 4357 5151 5171 4357 Size: 794 20 6027 Fragments arranged by size: 6027 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6806 DraI TTT'AAA Cuts at: 3373 4145 3373 Size: 772 6069 DraIII CAC_nnn'GTG Cuts at: 2548 3226 2548 Size: 678 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2558 5848 809 Size: 507 1242 3290 1802 Fragments arranged by size: 3290 1802 1242 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4134 4288 5177 Size: 54 1425 50 275 18 2304 154 889 Cuts at: 5177 6806 8 Size: 1629 43 Fragments arranged by size: 2304 1629 1425 889 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4288 1537 Size: 275 2476 4090 Fragments arranged by size: 4090 2476 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2529 3347 3933 3944 4771 1353 Size: 1176 818 586 11 827 3423 Fragments arranged by size: 3423 1176 827 818 586 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5428 5802 5948 387 Size: 1064 138 3839 374 146 1280 Fragments arranged by size: 3839 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2216 2216 Size: 6841 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2950 3328 3512 3906 4904 5501 6288 Size: 393 1318 378 184 394 998 597 787 Cuts at: 6288 6556 1239 Size: 268 1524 Fragments arranged by size: 1524 1318 998 787 597 394 393 378 268 184 EcoO109I rG'GnC_Cy Cuts at: 2965 2965 Size: 6841 EcoRI G'AATT_C Cuts at: 4150 4528 4150 Size: 378 6463 EcoRV GAT'ATC Cuts at: 3436 3436 Size: 6841 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3606 3764 3606 Size: 158 6683 FspI TGC'GCA Cuts at: 5347 5347 Size: 6841 HaeII r_GCGC'y Cuts at: 1678 5988 1678 Size: 4310 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2601 2633 3044 Size: 32 135 32 825 32 172 32 411 Cuts at: 3044 3076 5482 5514 5556 5588 1373 Size: 32 2406 32 42 32 2626 Fragments arranged by size: 2626 2406 825 411 172 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 4185 4431 669 Size: 988 281 625 1622 246 3079 Fragments arranged by size: 3079 1622 988 625 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6841 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KpnI G_GTAC'C Cuts at: 4166 4166 Size: 6841 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4054 5955 6139 1305 Size: 2749 1901 184 2007 Fragments arranged by size: 2749 2007 1901 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4136 10 Size: 54 1425 343 2304 2715 Fragments arranged by size: 2715 2304 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2787 3812 4067 4799 5158 5317 518 Size: 648 1621 1025 255 732 359 159 2042 Fragments arranged by size: 2042 1621 1025 732 648 359 255 159 MspA1I CmG'CkG Cuts at: 1606 2816 3916 4257 4934 6082 6327 1606 Size: 1210 1100 341 677 1148 245 2120 Fragments arranged by size: 2120 1210 1148 1100 677 341 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6289 NdeI CA'TA_TG Cuts at: 387 387 Size: 6841 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6841 NotI GC'GGCC_GC Cuts at: 4288 4288 Size: 6841 NspI r_CATG'y Cuts at: 1132 1229 4199 5744 1132 Size: 97 2970 1545 2229 Fragments arranged by size: 2970 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6841 PciI A'CATG_T Cuts at: 5740 5740 Size: 6841 PflMI CCAn_nnn'nTGG Cuts at: 1108 2467 3226 3674 1108 Size: 1359 759 448 4275 Fragments arranged by size: 4275 1359 759 448 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4910 4942 6423 6455 4910 Size: 32 1481 32 5296 Fragments arranged by size: 5296 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2965 2965 Size: 6841 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6841 PsiI TTA'TAA Cuts at: 4411 4411 Size: 6841 PstI C_TGCA'G Cuts at: 830 2596 3094 4193 830 Size: 1766 498 1099 3478 Fragments arranged by size: 3478 1766 1099 498 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 PvuII CAG'CTG Cuts at: 3916 3916 Size: 6841 SacI G_AGCT'C Cuts at: 2218 2218 Size: 6841 SalI G'TCGA_C Cuts at: 1936 4183 1936 Size: 2247 4594 SapI GCTCTTCn'nnn_ Cuts at: 2529 3933 2529 Size: 1404 5437 SbfI CC_TGCA'GG Cuts at: 4193 4193 Size: 6841 ScaI AGT'ACT Cuts at: 5089 6801 5089 Size: 1712 5129 SexAI A'CCwGG_T Cuts at: 3222 3222 Size: 6841 SfcI C'TryA_G Cuts at: 826 1045 2592 3090 4189 5324 6005 6196 826 Size: 219 1547 498 1099 1135 681 191 1471 Fragments arranged by size: 1547 1471 1135 1099 681 498 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 4168 1872 Size: 2296 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3127 3331 4945 5846 6108 820 Size: 197 926 1184 204 1614 901 262 1553 Fragments arranged by size: 1614 1553 1184 926 901 262 204 197 SnaBI TAC'GTA Cuts at: 493 4632 493 Size: 4139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 4199 1132 Size: 3067 3774 SspI AAT'ATT Cuts at: 5 52 3511 3715 3906 4765 5 Size: 47 3459 204 191 859 2081 Fragments arranged by size: 3459 2081 859 204 191 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6841 StyI C'CwwG_G Cuts at: 513 1065 2340 3134 4280 513 Size: 552 1275 794 1146 3074 Fragments arranged by size: 3074 1275 1146 794 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 4868 4885 5038 5223 5668 325 Size: 1255 879 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 879 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3804 5087 6799 Size: 274 80 33 51 1443 1827 1283 1712 Cuts at: 6799 96 Size: 138 Fragments arranged by size: 1827 1712 1443 1283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6801 6833 6801 Size: 32 6809 Tth111I GACn'n_nGTC Cuts at: 1316 2179 1316 Size: 863 5978 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 2468 Size: 6841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6841 XmaI C'CCGG_G Cuts at: 1870 4166 1870 Size: 2296 4545 XmnI GAAnn'nnTTC Cuts at: 4970 4970 Size: 6841 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4649 276 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI