################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstPI BstUI Bsu36I CelII Cfr10I CpoI DraI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI Ksp632I KspI MamI MluI MroI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI StyI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 269 GGTACC G^GTACC AflIII 1094 ACATGT A^C[GA][CT]GT AseI 373 ATTAAT AT^TAAT AsnI 373 ATTAAT AT^TAAT Asp718I 269 GGTACC G^GTACC AvaI 224 CTCGAG C^[CT]CG[GA]G BalI 671 TGGCCA TGG^CCA BanI 269 GGTACC G^G[CT][GA]CC BbsI 69 GTCTTC GAAGAC(2/6) BbvI 135 GCAGC GCAGC(8/12) BfaI 1286 CTAG C^TAG BpuAI 69 GTCTTC GAAGAC(2/6) BsaI 989 GAGACC GGTCTC(1/5) BsgI 1151 GTGCAG GTGCAG(16/14) BsmI 496 GAATGC GAATGC(1/-1) BsoBI 224 CTCGAG C^[CT]CG[GA]G Bsp1407I 230 TGTACA T^GTACA BspDI 392 ATCGAT AT^CGAT BspLU11I 1094 ACATGT A^CATGT BsrDI 1040 CATTGC GCAATG(2/0) BsrGI 230 TGTACA T^GTACA BssSI 1336 CTCGTG CACGAG(-5/-1) Bst1107I 807 GTATAC GTA^TAC BstBI 486 TTCGAA TT^CGAA BstXI 1298 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 807 GTATAC GTA^TAC CfoI 287 GCGC GCG^C ClaI 392 ATCGAT AT^CGAT DraII 998 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 675 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 622 GACCATCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 671 TGGCCA [CT]^GGCC[GA] EcoNI 756 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 998 AGGACCT [GA]G^G[ACGT]CC[CT] HaeII 286 AGCGCC [GA]GCGC^[CT] HhaI 287 GCGC GCG^C HinP1I 287 GCGC G^CGC HincII 407 GTCGAC GT[CT]^[GA]AC HindII 407 GTCGAC GT[CT]^[GA]AC KpnI 269 GGTACC GGTAC^C MaeI 1286 CTAG C^TAG MaeII 711 ACGT A^CGT MfeI 158 CAATTG C^AATTG MluNI 671 TGGCCA TGG^CCA MscI 671 TGGCCA TGG^CCA MunI 158 CAATTG C^AATTG NspV 486 TTCGAA TT^CGAA PaeR7I 224 CTCGAG C^TCGAG PpuMI 998 AGGACCT [GA]G^G[AT]CC[CT] PshBI 373 ATTAAT AT^TAAT SalI 407 GTCGAC G^TCGAC SexAI 802 ACCAGGT A^CC[AT]GGT SfuI 486 TTCGAA TT^CGAA SspBI 230 TGTACA T^GTACA StuI 957 AGGCCT AGG^CCT TseI 135 GCAGC G^C[AT]GC XhoI 224 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 407 GTCGAC GT^[AC][GT]AC 807 GTATAC AfaI 231 GTAC GT^AC 270 GTAC AlwI 549 GATCC GGATC(4/5) 1189 GGATC AlwNI 285 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 922 CAGCTCCTG AspHI 171 GAGCAC G[AT]GC[AT]^C 1276 GAGCAC BglII 1106 AGATCT A^GATCT 1125 AGATCT BmyI 171 GAGCAC G[AGT]GC[ACT]^C 1276 GAGCAC BsaJI 34 CCTGGG C^C[ACGT][ACGT]GG 642 CCTGGG BsiHKAI 171 GAGCAC G[AT]GC[AT]^C 1276 GAGCAC BsmAI 481 GAGAC GTCTC(1/5) 989 GAGAC BsmFI 653 GGGAC GGGAC(10/14) 193 GTCCC Bsp1286I 171 GAGCAC G[AGT]GC[ACT]^C 1276 GAGCAC EcoRI 609 GAATTC G^AATTC 1224 GAATTC Fnu4HI 135 GCAGC GC^[ACGT]GC 766 GCCGC ItaI 135 GCAGC GC^[ACGT]GC 766 GCCGC MspA1I 521 CAGCTG C[AC]G^C[GT]G 589 CAGCTG NlaIV 269 GGTACC GG[ACGT]^[ACGT]CC 1008 GGAACC PvuII 521 CAGCTG CAG^CTG 589 CAGCTG RsaI 231 GTAC GT^AC 270 GTAC SmlI 224 CTCGAG C^T[CT][GA]AG 1003 CTTGAG SphI 77 GCATGC GCATG^C 103 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AcsI AluI ApoI AvaII BpmI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NspI PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I XhoII