(Circular) (Six-base) MAPSORT of: pFN21AB5810.seq Check: 6040 from: 1 to: 6049 pFN21AB5810 6049 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2413 2813 3392 1937 Size: 476 400 579 4594 Fragments arranged by size: 4594 579 476 400 Acc65I G'GTAC_C Cuts at: 2274 3370 2274 Size: 1096 4953 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 2786 3255 4112 5496 5764 1632 Size: 466 688 469 857 1384 268 1917 Fragments arranged by size: 1917 1384 857 688 469 466 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 3099 4948 5925 1203 Size: 1896 1849 977 1327 Fragments arranged by size: 1896 1849 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 2295 2932 3792 5364 2295 Size: 637 860 1572 2980 Fragments arranged by size: 2980 1572 860 637 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 2614 2663 2799 3063 3186 3229 3358 3584 Size: 1121 49 136 264 123 43 129 226 Cuts at: 3584 3736 5845 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1121 264 226 152 136 129 123 49 43 AseI AT'TA_AT Cuts at: 160 1032 2379 4603 160 Size: 872 1347 2224 1606 Fragments arranged by size: 2224 1606 1347 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2229 3374 1870 Size: 73 286 1145 4545 Fragments arranged by size: 4545 1145 286 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 3370 4825 618 Size: 325 217 627 487 1096 1455 1842 Fragments arranged by size: 1842 1455 1096 627 487 325 217 BanII G_rGCy'C Cuts at: 1391 3368 1391 Size: 1977 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2067 928 Size: 971 168 4910 Fragments arranged by size: 4910 971 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4660 136 Size: 107 122 71 631 605 2988 1525 Fragments arranged by size: 2988 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3111 3130 6044 3111 Size: 19 2914 3116 Fragments arranged by size: 3116 2914 19 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 4110 5266 1790 Size: 2320 1156 2573 Fragments arranged by size: 2573 2320 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3780 4738 442 Size: 416 53 83 399 24 2363 958 1753 Fragments arranged by size: 2363 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2402 3245 4709 1239 Size: 833 330 843 1464 2579 Fragments arranged by size: 2579 1464 843 833 330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3029 4174 5039 5337 3029 Size: 1145 865 298 3741 Fragments arranged by size: 3741 1145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2988 4712 882 Size: 617 1489 1724 2219 Fragments arranged by size: 2219 1724 1489 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3856 4238 4873 275 Size: 53 83 186 936 2323 382 635 1451 Fragments arranged by size: 2323 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5824 Fragments arranged by size: 5824 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6049 BseYI C'CCAG_C Cuts at: 2596 3086 3302 5252 5748 2596 Size: 490 216 1950 496 2897 Fragments arranged by size: 2897 1950 496 490 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3177 1163 Size: 573 1441 4035 Fragments arranged by size: 4035 1441 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2180 3285 4110 4195 5266 2180 Size: 1105 825 85 1071 2963 Fragments arranged by size: 2963 1105 1071 825 85 BsmI GAATG_Cn' Cuts at: 2507 3560 3653 2507 Size: 1053 93 4903 Fragments arranged by size: 4903 1053 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 3285 3368 4110 4195 5266 1391 Size: 399 390 1105 83 742 85 1071 2174 Fragments arranged by size: 2174 1105 1071 742 399 390 85 83 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3404 844 Size: 2560 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3936 1394 Size: 429 2113 3507 Fragments arranged by size: 3507 2113 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3044 4544 4718 65 Size: 1183 1796 1500 174 1396 Fragments arranged by size: 1796 1500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2235 96 Size: 2139 3910 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 3341 4109 5121 3341 Size: 768 1012 4269 Fragments arranged by size: 4269 1012 768 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6049 BstBI TT'CG_AA Cuts at: 2492 4853 2492 Size: 2361 3688 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3310 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2553 3111 3130 3379 3746 4131 4148 5600 Size: 581 558 19 249 367 385 17 1452 Cuts at: 5600 5686 5698 6044 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 581 558 385 367 346 249 86 19 17 12 BstZ17I GTA'TAC Cuts at: 2814 2814 Size: 6049 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5497 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2728 487 Size: 683 56 1502 3808 Fragments arranged by size: 3808 1502 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2151 3565 4359 4379 2061 Size: 90 1414 794 20 3731 Fragments arranged by size: 3731 1414 794 90 20 ClaI AT'CG_AT Cuts at: 1554 2398 1554 Size: 844 5205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2685 2685 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2633 5056 809 Size: 507 1317 2423 1802 Fragments arranged by size: 2423 1802 1317 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2676 3496 4385 Size: 54 1425 50 275 18 846 820 889 Cuts at: 4385 6014 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 846 820 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3979 1353 Size: 2626 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2098 2402 2786 3245 3255 4112 Size: 393 440 26 304 384 459 10 857 Cuts at: 4112 4709 5496 5764 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 857 787 597 459 440 393 384 304 268 26 10 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2765 Size: 6049 EcoO109I rG'GnC_Cy Cuts at: 3004 3004 Size: 6049 EcoRI G'AATT_C Cuts at: 2614 3229 3358 3736 2614 Size: 615 129 378 4927 Fragments arranged by size: 4927 615 378 129 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2295 5196 1678 Size: 617 2901 2531 Fragments arranged by size: 2901 2531 617 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2252 2284 4690 Size: 32 135 32 440 32 208 32 2406 Cuts at: 4690 4722 4764 4796 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2406 440 208 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2414 3393 3639 669 Size: 988 281 476 979 246 3079 Fragments arranged by size: 3079 988 979 476 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 2278 3374 2278 Size: 1096 4953 MfeI C'AATT_G Cuts at: 2163 3648 2163 Size: 1485 4564 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 2662 5163 5347 1305 Size: 1209 148 2501 184 2007 Fragments arranged by size: 2501 2007 1209 184 148 MscI TGG'CCA Cuts at: 10 64 1489 1832 2678 10 Size: 54 1425 343 846 3381 Fragments arranged by size: 3381 1425 846 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4007 4366 4525 518 Size: 648 2841 359 159 2042 Fragments arranged by size: 2841 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2528 2596 3465 4142 5290 5535 1606 Size: 922 68 869 677 1148 245 2120 Fragments arranged by size: 2120 1148 922 869 677 245 68 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5497 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2086 2112 3103 3407 4952 1132 Size: 97 857 26 991 304 1545 2229 Fragments arranged by size: 2229 1545 991 857 304 97 26 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 3099 4948 3099 Size: 1849 4200 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6049 PfoI T'CCnGG_A Cuts at: 2049 2088 3190 2049 Size: 39 1102 4908 Fragments arranged by size: 4908 1102 39 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3004 Size: 6049 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspXI vC'TCGA_Gb Cuts at: 2229 2229 Size: 6049 PstI C_TGCA'G Cuts at: 830 3401 830 Size: 2571 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2528 2596 2528 Size: 68 5981 SalI G'TCGA_C Cuts at: 1936 2412 3391 1936 Size: 476 979 4594 Fragments arranged by size: 4594 979 476 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 2807 2807 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 3397 4532 5213 5404 826 Size: 219 2352 1135 681 191 1471 Fragments arranged by size: 2352 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2229 3008 4153 5054 5316 820 Size: 197 926 286 779 1145 901 262 1553 Fragments arranged by size: 1553 1145 926 901 779 286 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 2086 2112 3407 1132 Size: 954 26 1295 3774 Fragments arranged by size: 3774 1295 954 26 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 2964 1517 Size: 1447 4602 StyI C'CwwG_G Cuts at: 513 1065 3488 513 Size: 552 2423 3074 Fragments arranged by size: 3074 2423 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3316 4076 4093 4246 4431 4876 325 Size: 1255 1736 760 17 153 185 445 1498 Fragments arranged by size: 1736 1498 1255 760 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 4295 6007 Size: 274 80 33 51 1443 258 2060 1712 Cuts at: 6007 96 Size: 138 Fragments arranged by size: 2060 1712 1443 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2053 2085 6009 6041 2053 Size: 32 3924 32 2061 Fragments arranged by size: 3924 2061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 2229 1943 Size: 286 5763 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI