(Circular) (Six-base) MAPSORT of: pFN21AE3567.seq Check: 8579 from: 1 to: 8026 pFN21AE3567 8026 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2544 2544 Size: 8026 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5836 278 Size: 53 83 186 936 4300 2468 Fragments arranged by size: 4300 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2133 5369 1937 Size: 196 3236 4594 Fragments arranged by size: 4594 3236 196 Acc65I G'GTAC_C Cuts at: 5347 5347 Size: 8026 AclI AA'CG_TT Cuts at: 1563 2699 2730 4695 6153 6526 1563 Size: 1136 31 1965 1458 373 3063 Fragments arranged by size: 3063 1965 1458 1136 373 31 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 3188 5152 5176 6089 7473 7741 1632 Size: 567 989 1964 24 913 1384 268 1917 Fragments arranged by size: 1964 1917 1384 989 913 567 268 24 AflII C'TTAA_G Cuts at: 820 1017 4255 820 Size: 197 3238 4591 Fragments arranged by size: 4591 3238 197 AflIII A'CryG_T Cuts at: 1203 6925 7902 1203 Size: 5722 977 1327 Fragments arranged by size: 5722 1327 977 AgeI A'CCGG_T Cuts at: 6857 6857 Size: 8026 AhdI GACnn_n'nnGTC Cuts at: 2755 6755 2755 Size: 4000 4026 AleI CACnn'nnGTG Cuts at: 1166 2972 3305 4745 1166 Size: 1806 333 1440 4447 Fragments arranged by size: 4447 1806 1440 333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4959 1184 Size: 3775 4251 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4551 4583 4551 Size: 32 7994 AlwNI CAG_nnn'CTG Cuts at: 4687 5769 7341 4687 Size: 1082 1572 5372 Fragments arranged by size: 5372 1572 1082 ApaI G_GGCC'C Cuts at: 3248 3248 Size: 8026 ApaLI G'TGCA_C Cuts at: 4791 6083 7239 4791 Size: 1292 1156 5578 Fragments arranged by size: 5578 1292 1156 ApoI r'AATT_y Cuts at: 1493 2279 2636 2827 3983 4321 4527 5335 5561 Size: 786 357 191 1156 338 206 808 226 Cuts at: 5561 5713 7822 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1156 808 786 357 338 226 206 191 152 AseI AT'TA_AT Cuts at: 160 1032 6580 160 Size: 872 5548 1606 Fragments arranged by size: 5548 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8026 AvaI C'yCGr_G Cuts at: 1870 1943 3781 4276 5351 1870 Size: 73 1838 495 1075 4545 Fragments arranged by size: 4545 1838 1075 495 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2400 2433 2400 Size: 33 7993 BamHI G'GATC_C Cuts at: 4888 5356 5723 4888 Size: 468 367 7191 Fragments arranged by size: 7191 468 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2328 2532 3377 4285 Size: 325 217 627 242 299 204 845 908 Cuts at: 4285 5347 6802 618 Size: 1062 1455 1842 Fragments arranged by size: 1842 1455 1062 908 845 627 325 299 242 217 204 BanII G_rGCy'C Cuts at: 1391 3248 3788 4092 4524 4930 5345 1391 Size: 1857 540 304 432 406 415 4072 Fragments arranged by size: 4072 1857 540 432 415 406 304 BbeI G_GCGC'C Cuts at: 2033 2033 Size: 8026 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2451 3159 3999 928 Size: 971 552 708 840 4955 Fragments arranged by size: 4955 971 840 708 552 BbvCI CC'TCA_GC Cuts at: 4707 4707 Size: 8026 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6217 6251 1903 Size: 34 50 34 4196 34 3678 Fragments arranged by size: 4196 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5918 7128 5918 Size: 1210 6816 BclI T'GATC_A Cuts at: 1548 3876 1548 Size: 2328 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 6637 136 Size: 107 122 71 631 605 439 4526 1525 Fragments arranged by size: 4526 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 3348 3639 8021 3348 Size: 291 4382 3353 Fragments arranged by size: 4382 3353 291 BlpI GC'TnA_GC Cuts at: 3290 5443 3290 Size: 2153 5873 Bme1580I G_kGCm'C Cuts at: 1790 2535 3248 4290 4795 6087 7243 1790 Size: 745 713 1042 505 1292 1156 2573 Fragments arranged by size: 2573 1292 1156 1042 745 713 505 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8026 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5757 6715 442 Size: 416 53 83 399 24 4340 958 1753 Fragments arranged by size: 4340 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8026 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4195 4195 Size: 8026 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2412 3421 3905 4297 4741 4807 4944 Size: 1046 127 1009 484 392 444 66 137 Cuts at: 4944 6686 1239 Size: 1742 2579 Fragments arranged by size: 2579 1742 1046 1009 484 444 392 137 127 66 Bpu10I CC'TnA_GC Cuts at: 2115 4707 2115 Size: 2592 5434 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3497 6151 7016 7314 3497 Size: 2654 865 298 4209 Fragments arranged by size: 4209 2654 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2322 3693 4081 4583 6689 882 Size: 617 823 1371 388 502 2106 2219 Fragments arranged by size: 2219 2106 1371 823 617 502 388 BsaAI yAC'GTr Cuts at: 493 1204 5817 493 Size: 711 4613 2702 Fragments arranged by size: 4613 2702 711 BsaBI GATnn'nnATC Cuts at: 8020 8020 Size: 8026 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 5066 5833 6215 Size: 53 83 186 936 497 3036 767 382 Cuts at: 6215 6850 275 Size: 635 1451 Fragments arranged by size: 3036 1451 936 767 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2602 6459 6857 7131 7278 7689 2602 Size: 3857 398 274 147 411 2939 Fragments arranged by size: 3857 2939 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 2488 2518 4551 Size: 30 165 30 955 30 100 30 2033 Cuts at: 4551 4581 1178 Size: 30 4623 Fragments arranged by size: 4623 2033 955 165 100 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 4163 4166 4169 4184 4524 4527 4601 Size: 1096 1881 3 3 15 340 3 74 Cuts at: 4601 4885 5101 5171 1186 Size: 284 216 70 4041 Fragments arranged by size: 4041 1881 1096 340 284 216 74 70 15 3 3 3 BseYI C'CCAG_C Cuts at: 2673 7229 7725 2673 Size: 4556 496 2974 Fragments arranged by size: 4556 2974 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3346 4180 1163 Size: 573 1610 834 5009 Fragments arranged by size: 5009 1610 834 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5476 6237 7265 1540 Size: 275 186 3475 761 1028 2301 Fragments arranged by size: 3475 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2774 3724 4795 4930 6087 6172 7243 2774 Size: 950 1071 135 1157 85 1071 3557 Fragments arranged by size: 3557 1157 1071 1071 950 135 85 BsmI GAATG_Cn' Cuts at: 5537 5630 5537 Size: 93 7933 BsmBI CGTCTCn'nnnn_ Cuts at: 2491 3328 2491 Size: 837 7189 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 2774 3248 3724 3788 4092 4290 Size: 399 745 239 474 476 64 304 198 Cuts at: 4290 4524 4795 4930 5345 6087 6172 7243 1391 Size: 234 271 135 415 742 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 476 474 415 399 304 271 239 234 198 135 85 64 BspEI T'CCGG_A Cuts at: 2602 7689 2602 Size: 5087 2939 BspHI T'CATG_A Cuts at: 3190 4033 4930 5915 7645 3190 Size: 843 897 985 1730 3571 Fragments arranged by size: 3571 1730 985 897 843 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2544 5381 844 Size: 1700 2837 3489 Fragments arranged by size: 3489 2837 1700 BsrBI CCG'CTC Cuts at: 1394 1823 3275 5913 1394 Size: 429 1452 2638 3507 Fragments arranged by size: 3507 2638 1452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 2787 6521 6695 65 Size: 1183 1504 35 3734 174 1396 Fragments arranged by size: 3734 1504 1396 1183 174 35 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 6670 6857 1700 Size: 110 198 4662 187 2869 Fragments arranged by size: 4662 2869 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8026 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8026 BssSI C'ACGA_G Cuts at: 2981 6086 7098 2981 Size: 3105 1012 3909 Fragments arranged by size: 3909 3105 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 3101 3523 794 Size: 1448 859 422 5297 Fragments arranged by size: 5297 1448 859 422 BstBI TT'CG_AA Cuts at: 6830 6830 Size: 8026 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8026 BstXI CCAn_nnnn'nTGG Cuts at: 4922 5217 4922 Size: 295 7731 BstYI r'GATC_y Cuts at: 1972 3348 3469 3639 4205 4546 4888 5213 5356 Size: 1376 121 170 566 341 342 325 143 Cuts at: 5356 5723 6108 6125 7577 7663 7675 8021 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1376 566 385 367 346 342 341 325 170 143 121 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3683 3683 Size: 8026 BtgI C'CryG_G Cuts at: 513 1065 3789 513 Size: 552 2724 4750 Fragments arranged by size: 4750 2724 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2041 487 Size: 683 56 815 6472 Fragments arranged by size: 6472 815 683 56 BtsI GCAGTG_nn' Cuts at: 2799 4477 5542 6336 6356 2799 Size: 1678 1065 794 20 4469 Fragments arranged by size: 4469 1678 1065 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8026 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2050 2085 529 Size: 35 1486 35 6470 Fragments arranged by size: 6470 1486 35 35 DraI TTT'AAA Cuts at: 2665 2826 5330 2665 Size: 161 2504 5361 Fragments arranged by size: 5361 2504 161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7033 809 Size: 507 5717 1802 Fragments arranged by size: 5717 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2857 5473 6362 Size: 54 1425 50 275 18 1027 2616 889 Cuts at: 6362 7991 8 Size: 1629 43 Fragments arranged by size: 2616 1629 1425 1027 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5473 1537 Size: 275 3661 4090 Fragments arranged by size: 4090 3661 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2539 4140 4161 4577 5119 5956 1353 Size: 833 353 1601 21 416 542 837 3423 Fragments arranged by size: 3423 1601 837 833 542 416 353 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6613 6987 7133 387 Size: 1064 138 5024 374 146 1280 Fragments arranged by size: 5024 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4928 4928 Size: 8026 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2285 2412 3188 3421 3905 4297 Size: 393 567 86 127 776 233 484 392 Cuts at: 4297 4741 4807 4944 5152 5176 6089 6686 7473 Size: 444 66 137 208 24 913 597 787 Cuts at: 7473 7741 1239 Size: 268 1524 Fragments arranged by size: 1524 913 787 776 597 567 484 444 393 392 268 233 208 137 127 86 66 24 EcoO109I rG'GnC_Cy Cuts at: 2475 3244 2475 Size: 769 7257 EcoRI G'AATT_C Cuts at: 2279 5335 5713 2279 Size: 3056 378 4592 Fragments arranged by size: 4592 3056 378 EcoRV GAT'ATC Cuts at: 4050 4050 Size: 8026 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3658 4224 4503 4865 3658 Size: 566 279 362 6819 Fragments arranged by size: 6819 566 362 279 FspI TGC'GCA Cuts at: 6532 6532 Size: 8026 HaeII r_GCGC'y Cuts at: 1678 2033 7173 1678 Size: 355 5140 2531 Fragments arranged by size: 5140 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2488 2520 2741 2773 3432 Size: 32 135 32 916 32 221 32 659 Cuts at: 3432 3464 4034 4066 4484 4516 4873 4880 4905 Size: 32 570 32 418 32 357 7 25 Cuts at: 4905 4912 6667 6699 6741 6773 1373 Size: 7 1755 32 42 32 2626 Fragments arranged by size: 2626 1755 916 659 570 418 357 221 135 42 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3556 5064 5370 5616 669 Size: 988 281 1618 1508 306 246 3079 Fragments arranged by size: 3079 1618 1508 988 306 281 246 HindIII A'AGCT_T Cuts at: 748 4258 748 Size: 3510 4516 HpaI GTT'AAC Cuts at: 5616 5616 Size: 8026 KasI G'GCGC_C Cuts at: 2029 2029 Size: 8026 KpnI G_GTAC'C Cuts at: 5351 5351 Size: 8026 MfeI C'AATT_G Cuts at: 5625 5625 Size: 8026 MluI A'CGCG_T Cuts at: 7902 7902 Size: 8026 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4210 7140 7324 1305 Size: 2905 2930 184 2007 Fragments arranged by size: 2930 2905 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2859 10 Size: 54 1425 343 1027 5177 Fragments arranged by size: 5177 1425 1027 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2326 2792 2972 3086 3305 3620 4038 Size: 648 1160 466 180 114 219 315 418 Cuts at: 4038 4745 5984 6343 6502 518 Size: 707 1239 359 159 2042 Fragments arranged by size: 2042 1239 1160 707 648 466 418 359 315 219 180 159 114 MspA1I CmG'CkG Cuts at: 1606 5442 6119 7267 7512 1606 Size: 3836 677 1148 245 2120 Fragments arranged by size: 3836 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2030 2030 Size: 8026 NcoI C'CATG_G Cuts at: 513 1065 3789 513 Size: 552 2724 4750 Fragments arranged by size: 4750 2724 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8026 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8026 NotI GC'GGCC_GC Cuts at: 5473 5473 Size: 8026 NspI r_CATG'y Cuts at: 1132 1229 5384 6929 1132 Size: 97 4155 1545 2229 Fragments arranged by size: 4155 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8026 PciI A'CATG_T Cuts at: 6925 6925 Size: 8026 PflMI CCAn_nnn'nTGG Cuts at: 1108 3224 4556 4687 1108 Size: 2116 1332 131 4447 Fragments arranged by size: 4447 2116 1332 131 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 8026 PmeI GTTT'AAAC Cuts at: 5330 5330 Size: 8026 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4551 4583 4912 4944 6095 6127 7608 7640 4551 Size: 32 329 32 1151 32 1481 32 4937 Fragments arranged by size: 4937 1481 1151 329 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2475 2475 Size: 8026 PshAI GACnn'nnGTC Cuts at: 1613 4431 1613 Size: 2818 5208 PsiI TTA'TAA Cuts at: 5596 5596 Size: 8026 PspOMI G'GGCC_C Cuts at: 3244 3244 Size: 8026 PstI C_TGCA'G Cuts at: 830 3033 4948 5076 5378 830 Size: 2203 1915 128 302 3478 Fragments arranged by size: 3478 2203 1915 302 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8026 SacI G_AGCT'C Cuts at: 4930 4930 Size: 8026 SalI G'TCGA_C Cuts at: 1936 5368 1936 Size: 3432 4594 SanDI GG'GwC_CC Cuts at: 2475 2475 Size: 8026 SbfI CC_TGCA'GG Cuts at: 5378 5378 Size: 8026 ScaI AGT'ACT Cuts at: 2376 3061 6274 7986 2376 Size: 685 3213 1712 2416 Fragments arranged by size: 3213 2416 1712 685 SexAI A'CCwGG_T Cuts at: 2906 3545 4998 2906 Size: 639 1453 5934 Fragments arranged by size: 5934 1453 639 SfcI C'TryA_G Cuts at: 826 1045 3029 4944 5072 5374 6509 7190 7381 Size: 219 1984 1915 128 302 1135 681 191 Cuts at: 7381 826 Size: 1471 Fragments arranged by size: 1984 1915 1471 1135 681 302 219 191 128 SfoI GGC'GCC Cuts at: 2031 2031 Size: 8026 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8026 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8026 SmaI CCC'GGG Cuts at: 1872 5353 1872 Size: 3481 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3512 4255 6130 7031 7293 820 Size: 197 926 1569 743 1875 901 262 1553 Fragments arranged by size: 1875 1569 1553 926 901 743 262 197 SnaBI TAC'GTA Cuts at: 493 5817 493 Size: 5324 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8026 SphI G_CATG'C Cuts at: 1132 5384 1132 Size: 4252 3774 SspI AAT'ATT Cuts at: 5 52 3533 5950 5 Size: 47 3481 2417 2081 Fragments arranged by size: 3481 2417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8026 StyI C'CwwG_G Cuts at: 513 1065 3237 3393 3789 4092 4436 5465 513 Size: 552 2172 156 396 303 344 1029 3074 Fragments arranged by size: 3074 2172 1029 552 396 344 303 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6053 6070 6223 6408 6853 325 Size: 1255 4473 17 153 185 445 1498 Fragments arranged by size: 4473 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2374 3059 3279 Size: 274 80 33 51 1443 397 685 220 Cuts at: 3279 3432 6272 7984 96 Size: 153 2840 1712 138 Fragments arranged by size: 2840 1712 1443 685 397 274 220 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7986 8018 7986 Size: 32 7994 Tth111I GACn'n_nGTC Cuts at: 1316 2450 2502 1316 Size: 1134 52 6840 Fragments arranged by size: 6840 1134 52 XbaI T'CTAG_A Cuts at: 5362 5362 Size: 8026 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2107 Size: 8026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8026 XmaI C'CCGG_G Cuts at: 1870 5351 1870 Size: 3481 4545 XmnI GAAnn'nnTTC Cuts at: 3479 3534 3568 3996 4695 6155 3479 Size: 55 34 428 699 1460 5350 Fragments arranged by size: 5350 1460 699 428 55 34 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5834 276 Size: 53 83 186 936 4300 2468 Fragments arranged by size: 4300 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI