(Linear) (Six-base) MAPSORT of: KIEE3567.seq Check: 2813 from: 1 to: 3321 KIEE3567.orf With 182 enzymes: SgfI * March 22, 2012 16:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 539 3321 Size: 539 2782 AccI GT'mk_AC Cuts at: 0 128 3321 Size: 128 3193 AclI AA'CG_TT Cuts at: 0 694 725 2690 3321 Size: 694 31 1965 631 Fragments arranged by size: 1965 694 631 31 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 1183 3147 3171 3321 Size: 194 989 1964 24 150 Fragments arranged by size: 1964 989 194 150 24 AflII C'TTAA_G Cuts at: 0 2250 3321 Size: 2250 1071 AhdI GACnn_n'nnGTC Cuts at: 0 750 3321 Size: 750 2571 AleI CACnn'nnGTG Cuts at: 0 967 1300 2740 3321 Size: 967 333 1440 581 Fragments arranged by size: 1440 967 581 333 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2954 3321 Size: 2954 367 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2546 2578 3321 Size: 2546 32 743 Fragments arranged by size: 2546 743 32 AlwNI CAG_nnn'CTG Cuts at: 0 2682 3321 Size: 2682 639 ApaI G_GGCC'C Cuts at: 0 1243 3321 Size: 1243 2078 ApaLI G'TGCA_C Cuts at: 0 2786 3321 Size: 2786 535 ApoI r'AATT_y Cuts at: 0 274 631 822 1978 2316 2522 3321 Size: 274 357 191 1156 338 206 799 Fragments arranged by size: 1156 799 357 338 274 206 191 AvaI C'yCGr_G Cuts at: 0 1776 2271 3321 Size: 1776 495 1050 Fragments arranged by size: 1776 1050 495 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 395 428 3321 Size: 395 33 2893 Fragments arranged by size: 2893 395 33 BamHI G'GATC_C Cuts at: 0 2883 3321 Size: 2883 438 BanI G'GyrC_C Cuts at: 0 24 323 527 1372 2280 3321 Size: 24 299 204 845 908 1041 Fragments arranged by size: 1041 908 845 299 204 24 BanII G_rGCy'C Cuts at: 0 1243 1783 2087 2519 2925 3321 Size: 1243 540 304 432 406 396 Fragments arranged by size: 1243 540 432 406 396 304 BbeI G_GCGC'C Cuts at: 0 28 3321 Size: 28 3293 BbsI GAAGACnn'nnnn_ Cuts at: 0 446 1154 1994 3321 Size: 446 708 840 1327 Fragments arranged by size: 1327 840 708 446 BbvCI CC'TCA_GC Cuts at: 0 2702 3321 Size: 2702 619 BclI T'GATC_A Cuts at: 0 1871 3321 Size: 1871 1450 BglI GCCn_nnn'nGGC Cuts at: 0 106 3321 Size: 106 3215 BglII A'GATC_T Cuts at: 0 1343 1634 3321 Size: 1343 291 1687 Fragments arranged by size: 1687 1343 291 BlpI GC'TnA_GC Cuts at: 0 1285 3321 Size: 1285 2036 Bme1580I G_kGCm'C Cuts at: 0 530 1243 2285 2790 3321 Size: 530 713 1042 505 531 Fragments arranged by size: 1042 713 531 530 505 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2190 3321 Size: 2190 1131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 407 1416 1900 2292 2736 2802 2939 Size: 280 127 1009 484 392 444 66 137 Cuts at: 2939 3321 Size: 382 Fragments arranged by size: 1009 484 444 392 382 280 137 127 66 Bpu10I CC'TnA_GC Cuts at: 0 110 2702 3321 Size: 110 2592 619 Fragments arranged by size: 2592 619 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1492 3321 Size: 1492 1829 BsaI GGTCTCn'nnnn_ Cuts at: 0 317 1688 2076 2578 3321 Size: 317 1371 388 502 743 Fragments arranged by size: 1371 743 502 388 317 BsaHI Gr'CG_yC Cuts at: 0 25 3061 3321 Size: 25 3036 260 Fragments arranged by size: 3036 260 25 BsaWI w'CCGG_w Cuts at: 0 597 3321 Size: 597 2724 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 353 383 483 513 2546 2576 3321 Size: 353 30 100 30 2033 30 745 Fragments arranged by size: 2033 745 353 100 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 277 2158 2161 2164 2179 2519 2522 2596 Size: 277 1881 3 3 15 340 3 74 Cuts at: 2596 2880 3096 3166 3321 Size: 284 216 70 155 Fragments arranged by size: 1881 340 284 277 216 155 74 70 15 3 3 3 BseYI C'CCAG_C Cuts at: 0 668 3321 Size: 668 2653 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1341 2175 3321 Size: 1341 834 1146 Fragments arranged by size: 1341 1146 834 BsiHKAI G_wGCw'C Cuts at: 0 769 1719 2790 2925 3321 Size: 769 950 1071 135 396 Fragments arranged by size: 1071 950 769 396 135 BsmBI CGTCTCn'nnnn_ Cuts at: 0 486 1323 3321 Size: 486 837 1998 Fragments arranged by size: 1998 837 486 Bsp1286I G_dGCh'C Cuts at: 0 530 769 1243 1719 1783 2087 2285 2519 Size: 530 239 474 476 64 304 198 234 Cuts at: 2519 2790 2925 3321 Size: 271 135 396 Fragments arranged by size: 530 476 474 396 304 271 239 234 198 135 64 BspEI T'CCGG_A Cuts at: 0 597 3321 Size: 597 2724 BspHI T'CATG_A Cuts at: 0 1185 2028 2925 3321 Size: 1185 843 897 396 Fragments arranged by size: 1185 897 843 396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 539 3321 Size: 539 2782 BsrBI CCG'CTC Cuts at: 0 1270 3321 Size: 1270 2051 BsrDI GCAATG_nn' Cuts at: 0 747 782 3321 Size: 747 35 2539 Fragments arranged by size: 2539 747 35 BsrFI r'CCGG_y Cuts at: 0 3 3321 Size: 3 3318 BssSI C'ACGA_G Cuts at: 0 976 3321 Size: 976 2345 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 1096 1518 3321 Size: 237 859 422 1803 Fragments arranged by size: 1803 859 422 237 BstXI CCAn_nnnn'nTGG Cuts at: 0 2917 3212 3321 Size: 2917 295 109 Fragments arranged by size: 2917 295 109 BstYI r'GATC_y Cuts at: 0 1343 1464 1634 2200 2541 2883 3208 3321 Size: 1343 121 170 566 341 342 325 113 Fragments arranged by size: 1343 566 342 341 325 170 121 113 Bsu36I CC'TnA_GG Cuts at: 0 1678 3321 Size: 1678 1643 BtgI C'CryG_G Cuts at: 0 1784 3321 Size: 1784 1537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 36 3321 Size: 36 3285 BtsI GCAGTG_nn' Cuts at: 0 794 2472 3321 Size: 794 1678 849 Fragments arranged by size: 1678 849 794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 45 80 3321 Size: 45 35 3241 Fragments arranged by size: 3241 45 35 DraI TTT'AAA Cuts at: 0 660 821 3321 Size: 660 161 2500 Fragments arranged by size: 2500 660 161 EaeI y'GGCC_r Cuts at: 0 852 3321 Size: 852 2469 EarI CTCTTCn'nnn_ Cuts at: 0 181 534 2135 2156 2572 3114 3321 Size: 181 353 1601 21 416 542 207 Fragments arranged by size: 1601 542 416 353 207 181 21 EcoICRI GAG'CTC Cuts at: 0 2923 3321 Size: 2923 398 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 280 407 1183 1416 1900 2292 2736 Size: 194 86 127 776 233 484 392 444 Cuts at: 2736 2802 2939 3147 3171 3321 Size: 66 137 208 24 150 Fragments arranged by size: 776 484 444 392 233 208 194 150 137 127 86 66 24 EcoO109I rG'GnC_Cy Cuts at: 0 470 1239 3321 Size: 470 769 2082 Fragments arranged by size: 2082 769 470 EcoRI G'AATT_C Cuts at: 0 274 3321 Size: 274 3047 EcoRV GAT'ATC Cuts at: 0 2045 3321 Size: 2045 1276 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1653 2219 2498 2860 3321 Size: 1653 566 279 362 461 Fragments arranged by size: 1653 566 461 362 279 HaeII r_GCGC'y Cuts at: 0 28 3321 Size: 28 3293 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 483 515 736 768 1427 1459 2029 2061 Size: 483 32 221 32 659 32 570 32 Cuts at: 2061 2479 2511 2868 2875 2900 2907 3321 Size: 418 32 357 7 25 7 414 Fragments arranged by size: 659 570 483 418 414 357 221 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 0 1551 3059 3321 Size: 1551 1508 262 Fragments arranged by size: 1551 1508 262 HindIII A'AGCT_T Cuts at: 0 2253 3321 Size: 2253 1068 KasI G'GCGC_C Cuts at: 0 24 3321 Size: 24 3297 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2205 3321 Size: 2205 1116 MscI TGG'CCA Cuts at: 0 854 3321 Size: 854 2467 MslI CAynn'nnrTG Cuts at: 0 321 787 967 1081 1300 1615 2033 2740 Size: 321 466 180 114 219 315 418 707 Cuts at: 2740 3321 Size: 581 Fragments arranged by size: 707 581 466 418 321 315 219 180 114 NarI GG'CG_CC Cuts at: 0 25 3321 Size: 25 3296 NcoI C'CATG_G Cuts at: 0 1784 3321 Size: 1784 1537 PflMI CCAn_nnn'nTGG Cuts at: 0 1219 2551 2682 3321 Size: 1219 1332 131 639 Fragments arranged by size: 1332 1219 639 131 PfoI T'CCnGG_A Cuts at: 0 41 3321 Size: 41 3280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2546 2578 2907 2939 3321 Size: 2546 32 329 32 382 Fragments arranged by size: 2546 382 329 32 32 PpuMI rG'GwC_Cy Cuts at: 0 470 3321 Size: 470 2851 PshAI GACnn'nnGTC Cuts at: 0 2426 3321 Size: 2426 895 PspOMI G'GGCC_C Cuts at: 0 1239 3321 Size: 1239 2082 PstI C_TGCA'G Cuts at: 0 1028 2943 3071 3321 Size: 1028 1915 128 250 Fragments arranged by size: 1915 1028 250 128 SacI G_AGCT'C Cuts at: 0 2925 3321 Size: 2925 396 SanDI GG'GwC_CC Cuts at: 0 470 3321 Size: 470 2851 ScaI AGT'ACT Cuts at: 0 371 1056 3321 Size: 371 685 2265 Fragments arranged by size: 2265 685 371 SexAI A'CCwGG_T Cuts at: 0 901 1540 2993 3321 Size: 901 639 1453 328 Fragments arranged by size: 1453 901 639 328 SfcI C'TryA_G Cuts at: 0 1024 2939 3067 3321 Size: 1024 1915 128 254 Fragments arranged by size: 1915 1024 254 128 SfoI GGC'GCC Cuts at: 0 26 3321 Size: 26 3295 SmlI C'TyrA_G Cuts at: 0 1507 2250 3321 Size: 1507 743 1071 Fragments arranged by size: 1507 1071 743 SspI AAT'ATT Cuts at: 0 1528 3321 Size: 1528 1793 StyI C'CwwG_G Cuts at: 0 1232 1388 1784 2087 2431 3321 Size: 1232 156 396 303 344 890 Fragments arranged by size: 1232 890 396 344 303 156 TatI w'GTAC_w Cuts at: 0 369 1054 1274 1427 3321 Size: 369 685 220 153 1894 Fragments arranged by size: 1894 685 369 220 153 Tth111I GACn'n_nGTC Cuts at: 0 445 497 3321 Size: 445 52 2824 Fragments arranged by size: 2824 445 52 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 102 3321 Size: 102 3219 XmnI GAAnn'nnTTC Cuts at: 0 1474 1529 1563 1991 2690 3321 Size: 1474 55 34 428 699 631 Fragments arranged by size: 1474 699 631 428 55 34 Enzymes that do cut: AarI AccI AclI AcuI AflII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI NarI NcoI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI SacI SanDI ScaI SexAI SfcI SfoI SmlI SspI StyI TatI Tth111I XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflIII AgeI AscI AseI AsiSI AvrII BcgI BciVI BfrBI BmgBI BmrI BmtI BsaAI BsaBI BsiEI BsiWI BsmI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraIII DrdI EagI EciI EcoNI FseI FspI FspAI HpaI KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI XbaI XhoI XmaI ZraI