################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I CpoI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 1411 AACGTT AA^CGTT AcsI 453 GAATTC [GA]^AATT[CT] AflIII 1377 ACACGT A^C[GA][CT]GT AhdI 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 936 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 453 GAATTC [GA]^AATT[CT] Asp700I 331 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 895 GGACC G^G[AT]CC BamHI 1240 GGATCC G^GATCC BciVI 1395 GGATAC GTATCC(6/5) BsaBI 1048 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 203 GAGACC GGTCTC(1/5) BsgI 1299 CTGCAC GTGCAG(16/14) BsmI 1370 GAATGC GAATGC(1/-1) Bsp1407I 66 TGTACA T^GTACA BspDI 496 ATCGAT AT^CGAT BspHI 48 TCATGA T^CATGA BsrGI 66 TGTACA T^GTACA BssKI 1133 CCTGG ^CC[ACGT]GG BstNI 1133 CCTGG CC^[AT]GG BstXI 1035 CCATATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1240 GGATCC [GA]^GATC[CT] CfoI 435 GCGC GCG^C ClaI 496 ATCGAT AT^CGAT DraI 415 TTTAAA TTT^AAA Eam1105I 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1306 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 453 GAATTC G^AATTC EcoRII 1133 CCTGG ^CC[AT]GG EcoRV 1165 GATATC GAT^ATC HhaI 435 GCGC GCG^C HinP1I 435 GCGC G^CGC HindIII 1460 AAGCTT A^AGCTT MamI 1048 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1240 GGATCC [GA]^GATC[CT] MspA1I 152 CAGCTG C[AC]G^C[GT]G MvaI 1133 CCTGG CC^[AT]GG NcoI 373 CCATGG C^CATGG NspI 688 ACATGC [GA]CATG^[CT] Psp1406I 1411 AACGTT AA^CGTT PvuII 152 CAGCTG CAG^CTG RcaI 48 TCATGA T^CATGA ScrFI 1133 CCTGG CC^[ACGT]GG SnaBI 1448 TACGTA TAC^GTA SspBI 66 TGTACA T^GTACA XcmI 791 CCAACTTTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1240 GGATCC [GA]^GATC[CT] XmnI 331 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbsI 352 GAAGAC GAAGAC(2/6) 1421 GTCTTC BpuAI 352 GAAGAC GAAGAC(2/6) 1421 GTCTTC BsaAI 1378 CACGTA [CT]AC^GT[GA] 1448 TACGTA Cac8I 1139 GCTTGC GC[ACGT]^[ACGT]GC 1273 GCTTGC Cfr13I 895 GGACC G^G[ACGT]CC 1010 GGGCC DsaI 207 CCGTGG C^C[GA][CT]GG 373 CCATGG EarI 859 GAAGAG CTCTTC(1/4) 1248 GAAGAG EcoT14I 373 CCATGG C^C[AT][AT]GG 1355 CCAAGG Ksp632I 859 GAAGAG CTCTTC(1/4) 1248 GAAGAG MslI 228 CATTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 823 CATGATAATG PflMI 976 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1013 CCACTGAATGG PleI 683 GAGTC GAGTC(4/5) 864 GAGTC PstI 155 CTGCAG CTGCA^G 1430 CTGCAG Sau96I 895 GGACC G^G[ACGT]CC 1010 GGGCC SfcI 155 CTGCAG C^T[GA][CT]AG 1430 CTGCAG StyI 373 CCATGG C^C[AT][AT]GG 1355 CCAAGG TaqI 497 TCGA T^CGA 971 TCGA TfiI 97 GAATC G^A[AT]TC 541 GATTC TthHB8I 497 TCGA T^CGA 971 TCGA Van91I 976 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1013 CCACTGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AluI AlwI AseI AsnI BbvI BfaI BsaJI BsiYI BslI BsmAI BsrDI BsrI BstF5I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV PshBI RsaI Sau3AI SfaNI SmlI Tru9I TseI Tsp45I Tsp509I TspRI