(Circular) (Six-base) MAPSORT of: pFN21AE3057.seq Check: 9804 from: 1 to: 5341 pFN21AE3057 5341 bp With 182 enzymes: SgfI * March 28, 2012 12:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2220 2220 Size: 5341 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3151 278 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2684 1937 Size: 747 4594 Acc65I G'GTAC_C Cuts at: 2662 2662 Size: 5341 AclI AA'CG_TT Cuts at: 1563 3468 3841 1563 Size: 1905 373 3063 Fragments arranged by size: 3063 1905 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3404 4788 5056 1632 Size: 1772 1384 268 1917 Fragments arranged by size: 1917 1772 1384 268 AfeI AGC'GCT Cuts at: 2091 2453 2091 Size: 362 4979 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5144 AflIII A'CryG_T Cuts at: 1203 4240 5217 1203 Size: 3037 977 1327 Fragments arranged by size: 3037 1327 977 AgeI A'CCGG_T Cuts at: 2321 4172 2321 Size: 1851 3490 AhdI GACnn_n'nnGTC Cuts at: 4070 4070 Size: 5341 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5341 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5341 AlwNI CAG_nnn'CTG Cuts at: 3084 4656 3084 Size: 1572 3769 ApaLI G'TGCA_C Cuts at: 2207 3398 4554 2207 Size: 1191 1156 2994 Fragments arranged by size: 2994 1191 1156 ApoI r'AATT_y Cuts at: 1493 2335 2650 2876 3028 5137 1493 Size: 842 315 226 152 2109 1697 Fragments arranged by size: 2109 1697 842 315 226 152 AseI AT'TA_AT Cuts at: 160 1032 3895 160 Size: 872 2863 1606 Fragments arranged by size: 2863 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 AvaI C'yCGr_G Cuts at: 1870 1943 2666 1870 Size: 73 723 4545 Fragments arranged by size: 4545 723 73 BamHI G'GATC_C Cuts at: 2671 3038 2671 Size: 367 4974 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2662 4117 618 Size: 325 217 627 537 338 1455 1842 Fragments arranged by size: 1842 1455 627 537 338 325 217 BanII G_rGCy'C Cuts at: 1391 2026 2660 1391 Size: 635 634 4072 Fragments arranged by size: 4072 635 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3532 3566 1903 Size: 34 1595 34 3678 Fragments arranged by size: 3678 1595 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3233 4443 3233 Size: 1210 4131 BclI T'GATC_A Cuts at: 1548 1548 Size: 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3952 136 Size: 107 122 71 631 605 337 1943 1525 Fragments arranged by size: 1943 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5336 5336 Size: 5341 BlpI GC'TnA_GC Cuts at: 2758 2758 Size: 5341 Bme1580I G_kGCm'C Cuts at: 1790 2211 3402 4558 1790 Size: 421 1191 1156 2573 Fragments arranged by size: 2573 1191 1156 421 BmgBI CAC'GTC Cuts at: 1318 2191 2368 1318 Size: 873 177 4291 Fragments arranged by size: 4291 873 177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3072 4030 442 Size: 416 53 83 399 24 1655 958 1753 Fragments arranged by size: 1753 1655 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5341 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 5341 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2387 4001 1239 Size: 818 330 1614 2579 Fragments arranged by size: 2579 1614 818 330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3466 4331 4629 3466 Size: 865 298 4178 Fragments arranged by size: 4178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4004 882 Size: 617 2505 2219 Fragments arranged by size: 2505 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3132 493 Size: 711 1928 2702 Fragments arranged by size: 2702 1928 711 BsaBI GATnn'nnATC Cuts at: 5335 5335 Size: 5341 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 3148 3530 4165 Size: 53 83 186 936 1062 553 382 635 Cuts at: 4165 275 Size: 1451 Fragments arranged by size: 1451 1062 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 2321 2487 3774 4172 4446 4593 5004 2124 Size: 197 166 1287 398 274 147 411 2461 Fragments arranged by size: 2461 1287 411 398 274 197 166 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5116 Fragments arranged by size: 5116 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5341 BseYI C'CCAG_C Cuts at: 2232 2623 4544 5040 2232 Size: 391 1921 496 2533 Fragments arranged by size: 2533 1921 496 391 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4768 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2791 3552 4580 1540 Size: 275 186 790 761 1028 2301 Fragments arranged by size: 2301 1028 790 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2066 2211 2586 3402 3487 4558 2066 Size: 145 375 816 85 1071 2849 Fragments arranged by size: 2849 1071 816 375 145 85 BsmI GAATG_Cn' Cuts at: 2852 2945 2852 Size: 93 5248 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2066 2211 2586 2660 3402 3487 Size: 399 236 40 145 375 74 742 85 Cuts at: 3487 4558 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 236 145 85 74 40 BspEI T'CCGG_A Cuts at: 5004 5004 Size: 5341 BspHI T'CATG_A Cuts at: 2505 3230 4960 2505 Size: 725 1730 2886 Fragments arranged by size: 2886 1730 725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 2696 844 Size: 1376 476 3489 Fragments arranged by size: 3489 1376 476 BsrBI CCG'CTC Cuts at: 1394 1823 2479 3228 1394 Size: 429 656 749 3507 Fragments arranged by size: 3507 749 656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3836 4010 65 Size: 1183 2588 174 1396 Fragments arranged by size: 2588 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 3985 4172 1700 Size: 110 511 1664 187 2869 Fragments arranged by size: 2869 1664 511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5341 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5341 BssSI C'ACGA_G Cuts at: 3401 4413 3401 Size: 1012 4329 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5341 BstBI TT'CG_AA Cuts at: 4145 4145 Size: 5341 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5341 BstYI r'GATC_y Cuts at: 1972 2671 3038 3423 3440 4892 4978 4990 5336 Size: 699 367 385 17 1452 86 12 346 Cuts at: 5336 1972 Size: 1977 Fragments arranged by size: 1977 1452 699 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 513 Size: 552 939 96 3754 Fragments arranged by size: 3754 939 552 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4602 Fragments arranged by size: 4602 683 56 BtsI GCAGTG_nn' Cuts at: 2604 2857 3651 3671 2604 Size: 253 794 20 4274 Fragments arranged by size: 4274 794 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5306 DraI TTT'AAA Cuts at: 2645 2645 Size: 5341 DraIII CAC_nnn'GTG Cuts at: 2206 2206 Size: 5341 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4348 809 Size: 507 3032 1802 Fragments arranged by size: 3032 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2443 2788 3677 Size: 54 1425 50 275 18 613 345 889 Cuts at: 3677 5306 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 613 345 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2788 1537 Size: 275 976 4090 Fragments arranged by size: 4090 976 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 3271 1353 Size: 701 1217 3423 Fragments arranged by size: 3423 1217 701 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2432 3928 4302 4448 387 Size: 1064 138 843 1496 374 146 1280 Fragments arranged by size: 1496 1280 1064 843 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2387 3404 4001 4788 5056 1239 Size: 393 425 330 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 425 393 330 268 EcoRI G'AATT_C Cuts at: 2650 3028 2650 Size: 378 4963 FspI TGC'GCA Cuts at: 3847 3847 Size: 5341 HaeII r_GCGC'y Cuts at: 1678 2093 2455 2486 4488 1678 Size: 415 362 31 2002 2531 Fragments arranged by size: 2531 2002 415 362 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3982 4014 4056 4088 1373 Size: 32 135 32 2410 32 42 32 2626 Fragments arranged by size: 2626 2410 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2685 2931 669 Size: 988 281 747 246 3079 Fragments arranged by size: 3079 988 747 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5341 HpaI GTT'AAC Cuts at: 2931 2931 Size: 5341 KpnI G_GTAC'C Cuts at: 2666 2666 Size: 5341 MfeI C'AATT_G Cuts at: 2940 2940 Size: 5341 MluI A'CGCG_T Cuts at: 5217 5217 Size: 5341 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4455 4639 1305 Size: 3150 184 2007 Fragments arranged by size: 3150 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2445 10 Size: 54 1425 343 613 2906 Fragments arranged by size: 2906 1425 613 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2579 3299 3658 3817 518 Size: 648 1413 720 359 159 2042 Fragments arranged by size: 2042 1413 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2413 2623 2757 3434 4582 4827 1606 Size: 496 311 210 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 496 311 245 210 134 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3850 Fragments arranged by size: 3850 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5341 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5341 NotI GC'GGCC_GC Cuts at: 2788 2788 Size: 5341 NspI r_CATG'y Cuts at: 1132 1229 2699 4244 1132 Size: 97 1470 1545 2229 Fragments arranged by size: 2229 1545 1470 97 PacI TTA_AT'TAA Cuts at: 2253 2253 Size: 5341 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5341 PciI A'CATG_T Cuts at: 4240 4240 Size: 5341 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5341 PfoI T'CCnGG_A Cuts at: 2130 2472 2130 Size: 342 4999 PmeI GTTT'AAAC Cuts at: 2645 2645 Size: 5341 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3410 3442 4923 4955 3410 Size: 32 1481 32 3796 Fragments arranged by size: 3796 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2282 1613 Size: 669 4672 PsiI TTA'TAA Cuts at: 2911 2911 Size: 5341 PstI C_TGCA'G Cuts at: 830 2693 830 Size: 1863 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5341 PvuII CAG'CTG Cuts at: 2413 2413 Size: 5341 SacII CC_GC'GG Cuts at: 2103 2103 Size: 5341 SalI G'TCGA_C Cuts at: 1936 2683 1936 Size: 747 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5341 SbfI CC_TGCA'GG Cuts at: 2693 2693 Size: 5341 ScaI AGT'ACT Cuts at: 3589 5301 3589 Size: 1712 3629 SexAI A'CCwGG_T Cuts at: 2388 2388 Size: 5341 SfcI C'TryA_G Cuts at: 826 1045 2689 3824 4505 4696 826 Size: 219 1644 1135 681 191 1471 Fragments arranged by size: 1644 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5341 SgrAI Cr'CCGG_yG Cuts at: 1700 2321 1700 Size: 621 4720 SmaI CCC'GGG Cuts at: 1872 2668 1872 Size: 796 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3445 4346 4608 820 Size: 197 926 1502 901 262 1553 Fragments arranged by size: 1553 1502 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3132 493 Size: 2639 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5341 SphI G_CATG'C Cuts at: 1132 2699 1132 Size: 1567 3774 SspI AAT'ATT Cuts at: 5 52 3265 5 Size: 47 3213 2081 Fragments arranged by size: 3213 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5341 StyI C'CwwG_G Cuts at: 513 1065 2004 2328 2780 513 Size: 552 939 324 452 3074 Fragments arranged by size: 3074 939 552 452 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3368 3385 3538 3723 4168 325 Size: 1255 1788 17 153 185 445 1498 Fragments arranged by size: 1788 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3587 5299 96 Size: 274 80 33 51 1443 1610 1712 138 Fragments arranged by size: 1712 1610 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 5301 5333 2078 Size: 32 3191 32 2086 Fragments arranged by size: 3191 2086 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2390 1316 Size: 1074 4267 XbaI T'CTAG_A Cuts at: 2677 2677 Size: 5341 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2386 Size: 5341 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5341 XmaI C'CCGG_G Cuts at: 1870 2666 1870 Size: 796 4545 XmnI GAAnn'nnTTC Cuts at: 3470 3470 Size: 5341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3149 276 Size: 53 83 186 936 1615 2468 Fragments arranged by size: 2468 1615 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SfiI SfoI SrfI SwaI