################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BseRI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AhdI 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 210 GTGCAC G^TGCAC AlwI 759 GATCC GGATC(4/5) ApaLI 210 GTGCAC G^TGCAC AspEI 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 836 TGGCCA TGG^CCA BbrPI 700 CACGTG CAC^GTG BbsI 562 GTCTTC GAAGAC(2/6) BcnI 497 CCCGG CC^[GC]GG BfaI 97 CTAG C^TAG BpuAI 562 GTCTTC GAAGAC(2/6) BsaAI 700 CACGTG [CT]AC^GT[GA] BsaI 160 GAGACC GGTCTC(1/5) BsiEI 401 CGGCCG CG[GA][CT]^CG BsmBI 754 GAGACG CGTCTC(1/5) BsmFI 384 GTCCC GGGAC(10/14) Bsp1407I 773 TGTACA T^GTACA BspMI 542 ACCTGC ACCTGC(4/8) BsrGI 773 TGTACA T^GTACA BsrI 864 ACTGG ACTGG(1/-1) EagI 401 CGGCCG C^GGCCG Eam1105I 756 GACGATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 401 CGGCCG C^GGCCG Eco52I 401 CGGCCG C^GGCCG HaeII 120 GGCGCT [GA]GCGC^[CT] HapII 498 CCGG C^CGG HpaII 498 CCGG C^CGG KspI 372 CCGCGG CCGC^GG MaeI 97 CTAG C^TAG MboII 563 TCTTC GAAGA(8/7) MluNI 836 TGGCCA TGG^CCA MscI 836 TGGCCA TGG^CCA MspI 498 CCGG C^CGG NciI 497 CCCGG CC^[GC]GG NcoI 515 CCATGG C^CATGG NspI 143 GCATGC [GA]CATG^[CT] PleI 904 GACTC GAGTC(4/5) PmaCI 700 CACGTG CAC^GTG PmlI 700 CACGTG CAC^GTG SacII 372 CCGCGG CCGC^GG SphI 143 GCATGC GCATG^C SspBI 773 TGTACA T^GTACA StuI 99 AGGCCT AGG^CCT Tsp509I 505 AATT ^AATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 300 GTAC GT^AC 774 GTAC AspHI 210 GTGCAC G[AT]GC[AT]^C 492 GAGCAC BanI 7 GGCACC G^G[CT][GA]CC 356 GGCACC BpmI 27 CTCCAG CTGGAG(16/14) 751 CTGGAG BsiHKAI 210 GTGCAC G[AT]GC[AT]^C 492 GAGCAC BstAPI 231 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 357 GCACCCGCTGC DpnII 54 GATC ^GATC 759 GATC EcoT14I 515 CCATGG C^C[AT][AT]GG 839 CCAAGG HphI 582 GGTGA GGTGA(8/7) 38 TCACC MaeII 614 ACGT A^CGT 701 ACGT MboI 54 GATC ^GATC 759 GATC NdeII 54 GATC ^GATC 759 GATC RsaI 300 GTAC GT^AC 774 GTAC Sau3AI 54 GATC ^GATC 759 GATC StyI 515 CCATGG C^C[AT][AT]GG 839 CCAAGG TfiI 465 GATTC G^A[AT]TC 536 GATTC XcmI 179 CCAACCTGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 800 CCAACTGCTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BbvI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI HaeIII HgaI HhaI Hin1I HinP1I HinfI ItaI MaeIII MnlI MslI MspA1I MvaI MvnI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI Tsp45I TspRI