(Circular) (Six-base) MAPSORT of: pFN21AB6295.seq Check: 6047 from: 1 to: 7129 pFN21AB6295 7129 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3083 3083 Size: 7129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4939 278 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2689 2873 3618 3842 4472 1937 Size: 752 184 745 224 630 4594 Fragments arranged by size: 4594 752 745 630 224 184 Acc65I G'GTAC_C Cuts at: 4450 4450 Size: 7129 AclI AA'CG_TT Cuts at: 1563 5256 5629 1563 Size: 3693 373 3063 Fragments arranged by size: 3693 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2730 2747 3081 5192 6576 6844 1632 Size: 1098 17 334 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 1098 334 268 17 AfeI AGC'GCT Cuts at: 2171 2171 Size: 7129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 6028 7005 1203 Size: 4825 977 1327 Fragments arranged by size: 4825 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 3853 5858 3853 Size: 2005 5124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2480 4195 1184 Size: 1296 1715 4118 Fragments arranged by size: 4118 1715 1296 AlwNI CAG_nnn'CTG Cuts at: 2068 3578 4004 4872 6444 2068 Size: 1510 426 868 1572 2753 Fragments arranged by size: 2753 1572 1510 868 426 ApaLI G'TGCA_C Cuts at: 3189 5186 6342 3189 Size: 1997 1156 3976 Fragments arranged by size: 3976 1997 1156 ApoI r'AATT_y Cuts at: 1493 2660 2770 3126 3428 4438 4664 4816 6925 Size: 1167 110 356 302 1010 226 152 2109 Cuts at: 6925 1493 Size: 1697 Fragments arranged by size: 2109 1697 1167 1010 356 302 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 2747 4069 4454 1870 Size: 73 804 1322 385 4545 Fragments arranged by size: 4545 1322 804 385 73 AvrII C'CTAG_G Cuts at: 2890 3079 2890 Size: 189 6940 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2815 2848 2815 Size: 33 7096 BamHI G'GATC_C Cuts at: 4459 4826 4459 Size: 367 6762 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4130 4330 4450 5905 618 Size: 325 217 627 2343 200 120 1455 1842 Fragments arranged by size: 2343 1842 1455 627 325 217 200 120 BanII G_rGCy'C Cuts at: 1391 2054 3456 3654 4448 1391 Size: 663 1402 198 794 4072 Fragments arranged by size: 4072 1402 794 663 198 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2166 2220 3069 3713 4173 4419 928 Size: 971 267 54 849 644 460 246 3638 Fragments arranged by size: 3638 971 849 644 460 267 246 54 BbvCI CC'TCA_GC Cuts at: 4005 4005 Size: 7129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5320 5354 1903 Size: 34 3383 34 3678 Fragments arranged by size: 3678 3383 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2816 5021 6231 2816 Size: 2205 1210 3714 Fragments arranged by size: 3714 2205 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2327 5740 136 Size: 107 122 71 631 605 655 3413 1525 Fragments arranged by size: 3413 1525 655 631 605 122 107 71 BglII A'GATC_T Cuts at: 7124 7124 Size: 7129 BlpI GC'TnA_GC Cuts at: 2541 3450 4546 2541 Size: 909 1096 5124 Fragments arranged by size: 5124 1096 909 Bme1580I G_kGCm'C Cuts at: 1790 3193 4353 5190 6346 1790 Size: 1403 1160 837 1156 2573 Fragments arranged by size: 2573 1403 1160 1156 837 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3743 4860 5818 Size: 416 53 83 399 24 2326 1117 958 Cuts at: 5818 442 Size: 1753 Fragments arranged by size: 2326 1753 1117 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 3159 2191 Size: 968 6161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2624 3552 5789 1239 Size: 920 465 928 2237 2579 Fragments arranged by size: 2579 2237 928 920 465 Bpu10I CC'TnA_GC Cuts at: 3088 4005 3088 Size: 917 6212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2852 3152 3239 5254 6119 6417 2852 Size: 300 87 2015 865 298 3564 Fragments arranged by size: 3564 2015 865 300 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2257 2298 5792 882 Size: 617 758 41 3494 2219 Fragments arranged by size: 3494 2219 758 617 41 BsaAI yAC'GTr Cuts at: 493 1204 4920 493 Size: 711 3716 2702 Fragments arranged by size: 3716 2702 711 BsaBI GATnn'nnATC Cuts at: 7123 7123 Size: 7129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4936 5318 5953 275 Size: 53 83 186 936 3403 382 635 1451 Fragments arranged by size: 3403 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3937 5562 5960 6234 6381 6792 3937 Size: 1625 398 274 147 411 4274 Fragments arranged by size: 4274 1625 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3196 3226 1178 Size: 30 165 30 1793 30 5081 Fragments arranged by size: 5081 1793 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4022 4398 1186 Size: 2836 376 3917 Fragments arranged by size: 3917 2836 376 BseYI C'CCAG_C Cuts at: 6332 6828 6332 Size: 496 6633 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4165 1163 Size: 573 2429 4127 Fragments arranged by size: 4127 2429 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 4579 5340 6368 1540 Size: 275 186 21 2557 761 1028 2301 Fragments arranged by size: 2557 2301 1028 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 3193 5190 5275 6346 3193 Size: 1997 85 1071 3976 Fragments arranged by size: 3976 1997 1071 85 BsmI GAATG_Cn' Cuts at: 4640 4733 4640 Size: 93 7036 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 3193 3456 3654 4353 4448 5190 Size: 399 264 1139 263 198 699 95 742 Cuts at: 5190 5275 6346 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1139 1071 742 699 399 264 263 198 95 85 BspEI T'CCGG_A Cuts at: 3937 6792 3937 Size: 2855 4274 BspHI T'CATG_A Cuts at: 3358 5018 6748 3358 Size: 1660 1730 3739 Fragments arranged by size: 3739 1730 1660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3083 3823 4484 844 Size: 2239 740 661 3489 Fragments arranged by size: 3489 2239 740 661 BsrBI CCG'CTC Cuts at: 1394 1823 2024 2143 5016 1394 Size: 429 201 119 2873 3507 Fragments arranged by size: 3507 2873 429 201 119 BsrDI GCAATG_nn' Cuts at: 65 1248 5624 5798 65 Size: 1183 4376 174 1396 Fragments arranged by size: 4376 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5773 5960 1700 Size: 110 3963 187 2869 Fragments arranged by size: 3963 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3991 96 Size: 3895 3234 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 3322 5189 6201 3322 Size: 1867 1012 4250 Fragments arranged by size: 4250 1867 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7129 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstXI CCAn_nnnn'nTGG Cuts at: 2908 2908 Size: 7129 BstYI r'GATC_y Cuts at: 1972 2121 2183 4459 4826 5211 5228 6680 6766 Size: 149 62 2276 367 385 17 1452 86 Cuts at: 6766 6778 7124 1972 Size: 12 346 1977 Fragments arranged by size: 2276 1977 1452 385 367 346 149 86 62 17 12 BstZ17I GTA'TAC Cuts at: 2690 2690 Size: 7129 Bsu36I CC'TnA_GG Cuts at: 2249 2249 Size: 7129 BtgI C'CryG_G Cuts at: 513 1065 2016 513 Size: 552 951 5626 Fragments arranged by size: 5626 951 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3681 487 Size: 683 56 2455 3935 Fragments arranged by size: 3935 2455 683 56 BtsI GCAGTG_nn' Cuts at: 3066 3291 3867 3925 4645 5439 5459 3066 Size: 225 576 58 720 794 20 4736 Fragments arranged by size: 4736 794 720 576 225 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 4433 4433 Size: 7129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6136 809 Size: 507 4820 1802 Fragments arranged by size: 4820 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 4576 5465 Size: 54 1425 50 275 18 189 2557 889 Cuts at: 5465 7094 8 Size: 1629 43 Fragments arranged by size: 2557 1629 1425 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 4576 1537 Size: 275 207 2557 4090 Fragments arranged by size: 4090 2557 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2502 2640 2847 2890 3294 3501 3822 Size: 900 249 138 207 43 404 207 321 Cuts at: 3822 4333 5059 1353 Size: 511 726 3423 Fragments arranged by size: 3423 900 726 511 404 321 249 207 207 138 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 5716 6090 6236 387 Size: 1064 138 498 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 498 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2624 2730 2747 3081 3552 5192 Size: 393 527 465 106 17 334 471 1640 Cuts at: 5192 5789 6576 6844 1239 Size: 597 787 268 1524 Fragments arranged by size: 1640 1524 787 597 527 471 465 393 334 268 106 17 EcoO109I rG'GnC_Cy Cuts at: 2722 2831 2886 3382 2722 Size: 109 55 496 6469 Fragments arranged by size: 6469 496 109 55 EcoRI G'AATT_C Cuts at: 3428 4438 4816 3428 Size: 1010 378 5741 Fragments arranged by size: 5741 1010 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2581 2581 Size: 7129 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 2173 6276 1678 Size: 495 4103 2531 Fragments arranged by size: 4103 2531 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 2276 2308 2335 Size: 32 135 32 638 32 34 32 27 Cuts at: 2335 2367 3038 3070 3196 3228 3839 3871 3874 Size: 32 671 32 126 32 611 32 3 Cuts at: 3874 3906 4310 4342 5770 5802 5844 5876 1373 Size: 32 404 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 671 638 611 404 135 126 42 34 32 32 32 32 32 32 32 32 32 32 32 32 27 3 HincII GTy'rAC Cuts at: 669 1657 1938 2874 3789 4473 4719 669 Size: 988 281 936 915 684 246 3079 Fragments arranged by size: 3079 988 936 915 684 281 246 HindIII A'AGCT_T Cuts at: 748 4290 748 Size: 3542 3587 HpaI GTT'AAC Cuts at: 3789 4719 3789 Size: 930 6199 KpnI G_GTAC'C Cuts at: 4454 4454 Size: 7129 MfeI C'AATT_G Cuts at: 4728 4728 Size: 7129 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2800 2826 3215 3252 3939 6243 6427 1305 Size: 1495 26 389 37 687 2304 184 2007 Fragments arranged by size: 2304 2007 1495 687 389 184 37 26 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5307 Fragments arranged by size: 5307 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 3342 5087 5446 5605 518 Size: 648 1062 1114 1745 359 159 2042 Fragments arranged by size: 2042 1745 1114 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2364 2620 3449 4004 4545 5222 6370 Size: 412 346 256 829 555 541 677 1148 Cuts at: 6370 6615 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 829 677 555 541 412 346 256 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6577 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 2019 4576 2019 Size: 2557 4572 NruI TCG'CGA Cuts at: 2118 2118 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 2227 2305 2467 4487 6032 1132 Size: 97 998 78 162 2020 1545 2229 Fragments arranged by size: 2229 2020 1545 998 162 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7129 PciI A'CATG_T Cuts at: 6028 6028 Size: 7129 PflMI CCAn_nnn'nTGG Cuts at: 1108 3234 1108 Size: 2126 5003 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2831 2886 3382 2831 Size: 55 496 6578 Fragments arranged by size: 6578 496 55 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7129 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PstI C_TGCA'G Cuts at: 830 2320 4153 4481 830 Size: 1490 1833 328 3478 Fragments arranged by size: 3478 1833 1490 328 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7129 PvuII CAG'CTG Cuts at: 2620 3449 4004 2620 Size: 829 555 5745 Fragments arranged by size: 5745 829 555 SacII CC_GC'GG Cuts at: 2019 2019 Size: 7129 SalI G'TCGA_C Cuts at: 1936 2872 4471 1936 Size: 936 1599 4594 Fragments arranged by size: 4594 1599 936 SanDI GG'GwC_CC Cuts at: 2886 2886 Size: 7129 SapI GCTCTTCn'nnn_ Cuts at: 2890 3294 3822 2890 Size: 404 528 6197 Fragments arranged by size: 6197 528 404 SbfI CC_TGCA'GG Cuts at: 4481 4481 Size: 7129 ScaI AGT'ACT Cuts at: 5377 7089 5377 Size: 1712 5417 SexAI A'CCwGG_T Cuts at: 2574 2574 Size: 7129 SfcI C'TryA_G Cuts at: 826 1045 2316 4149 4477 5612 6293 6484 826 Size: 219 1271 1833 328 1135 681 191 1471 Fragments arranged by size: 1833 1471 1271 1135 681 328 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 4456 1872 Size: 2584 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 2867 3131 3218 5233 6134 Size: 197 926 804 120 264 87 2015 901 Cuts at: 6134 6396 820 Size: 262 1553 Fragments arranged by size: 2015 1553 926 901 804 264 262 197 120 87 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7129 SphI G_CATG'C Cuts at: 1132 4487 1132 Size: 3355 3774 SspI AAT'ATT Cuts at: 5 52 5053 5 Size: 47 5001 2081 Fragments arranged by size: 5001 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7129 StyI C'CwwG_G Cuts at: 513 1065 2827 2890 3079 3456 4568 513 Size: 552 1762 63 189 377 1112 3074 Fragments arranged by size: 3074 1762 1112 552 377 189 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 3176 5156 5173 5326 5511 5956 Size: 1255 747 849 1980 17 153 185 445 Cuts at: 5956 325 Size: 1498 Fragments arranged by size: 1980 1498 1255 849 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3160 3476 3611 Size: 274 80 33 51 1443 1183 316 135 Cuts at: 3611 3991 4241 5375 7087 96 Size: 380 250 1134 1712 138 Fragments arranged by size: 1712 1443 1183 1134 380 316 274 250 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7089 7121 7089 Size: 32 7097 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7129 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3231 3231 Size: 7129 XhoI C'TCGA_G Cuts at: 1943 2747 1943 Size: 804 6325 XmaI C'CCGG_G Cuts at: 1870 4454 1870 Size: 2584 4545 XmnI GAAnn'nnTTC Cuts at: 3114 3428 5258 3114 Size: 314 1830 4985 Fragments arranged by size: 4985 1830 314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4937 276 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SfiI SfoI SrfI SwaI