(Circular) (Six-base) MAPSORT of: pFN21AB5838.seq Check: 901 from: 1 to: 6148 pFN21AB5838 6148 bp With 182 enzymes: SgfI * March 28, 2012 12:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3200 3200 Size: 6148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2798 3141 3491 1937 Size: 861 343 350 4594 Fragments arranged by size: 4594 861 350 343 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 2641 4275 4648 1563 Size: 1078 1634 373 3063 Fragments arranged by size: 3063 1634 1078 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3447 4211 5595 5863 1632 Size: 1815 764 1384 268 1917 Fragments arranged by size: 1917 1815 1384 764 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 2624 1166 Size: 1458 4690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2460 1184 Size: 1276 4872 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 3300 3457 3683 3835 5944 1493 Size: 1807 157 226 152 2109 1697 Fragments arranged by size: 2109 1807 1697 226 157 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3473 1870 Size: 73 1530 4545 Fragments arranged by size: 4545 1530 73 AvrII C'CTAG_G Cuts at: 2980 2980 Size: 6148 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3469 4924 618 Size: 325 217 627 1682 1455 1842 Fragments arranged by size: 1842 1682 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3467 1391 Size: 2076 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3360 928 Size: 971 1461 3716 Fragments arranged by size: 3716 1461 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BfrBI ATG'CAT Cuts at: 2437 3173 2437 Size: 736 5412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3246 6143 3246 Size: 2897 3251 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 4209 5365 1790 Size: 2419 1156 2573 Fragments arranged by size: 2573 2419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3879 4837 Size: 416 53 83 399 24 762 1700 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1700 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3110 3223 4808 1239 Size: 1028 843 113 1585 2579 Fragments arranged by size: 2579 1585 1028 843 113 Bpu10I CC'TnA_GC Cuts at: 2049 2049 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2836 3255 4581 4979 5253 5400 5811 2836 Size: 419 1326 398 274 147 411 3173 Fragments arranged by size: 3173 1326 419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5923 Fragments arranged by size: 5923 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3389 1186 Size: 2203 3945 BseYI C'CCAG_C Cuts at: 5351 5847 5351 Size: 496 5652 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 2963 1163 Size: 573 414 813 4348 Fragments arranged by size: 4348 813 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 2069 4209 4294 5365 2055 Size: 14 2140 85 1071 2838 Fragments arranged by size: 2838 2140 1071 85 14 BsmI GAATG_Cn' Cuts at: 2519 3659 3752 2519 Size: 1140 93 4915 Fragments arranged by size: 4915 1140 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2069 3467 4209 4294 5365 1391 Size: 399 265 14 1398 742 85 1071 2174 Fragments arranged by size: 2174 1398 1071 742 399 265 85 14 BspEI T'CCGG_A Cuts at: 3255 5811 3255 Size: 2556 3592 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3200 3503 844 Size: 2356 303 3489 Fragments arranged by size: 3489 2356 303 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2559 2856 4643 4817 65 Size: 1183 1311 297 1787 174 1396 Fragments arranged by size: 1787 1396 1311 1183 297 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 794 Size: 1265 4883 BstBI TT'CG_AA Cuts at: 3304 4952 3304 Size: 1648 4500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2455 Size: 6148 BstYI r'GATC_y Cuts at: 1972 3246 3478 3845 4230 4247 5699 5785 5797 Size: 1274 232 367 385 17 1452 86 12 Cuts at: 5797 6143 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1274 385 367 346 232 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3121 3121 Size: 6148 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4657 Fragments arranged by size: 4657 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3664 4458 4478 3664 Size: 794 20 5334 Fragments arranged by size: 5334 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3178 3595 4484 Size: 54 1425 50 275 18 1348 417 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1348 889 417 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2184 3155 3423 4078 1353 Size: 739 92 971 268 655 3423 Fragments arranged by size: 3423 971 739 655 268 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 3110 3223 3447 4211 4808 5595 Size: 393 635 843 113 224 764 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1524 843 787 764 635 597 393 268 224 113 EcoNI CCTnn'n_nnAGG Cuts at: 2580 2580 Size: 6148 EcoO109I rG'GnC_Cy Cuts at: 2977 2977 Size: 6148 EcoRI G'AATT_C Cuts at: 3300 3457 3835 3300 Size: 157 378 5613 Fragments arranged by size: 5613 378 157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 3268 2767 Size: 501 5647 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4789 4821 4863 4895 1373 Size: 32 135 32 3217 32 42 32 2626 Fragments arranged by size: 3217 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3492 3738 669 Size: 988 281 852 702 246 3079 Fragments arranged by size: 3079 988 852 702 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 2208 2331 2925 3747 2208 Size: 123 594 822 4609 Fragments arranged by size: 4609 822 594 123 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2293 2979 5262 5446 1305 Size: 988 686 2283 184 2007 Fragments arranged by size: 2283 2007 988 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 2453 2624 4106 4465 4624 518 Size: 648 1276 11 171 1482 359 159 2042 Fragments arranged by size: 2042 1482 1276 648 359 171 159 11 MspA1I CmG'CkG Cuts at: 1606 3564 4241 5389 5634 1606 Size: 1958 677 1148 245 2120 Fragments arranged by size: 2120 1958 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4657 Fragments arranged by size: 4657 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 2439 3175 2439 Size: 736 5412 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PfoI T'CCnGG_A Cuts at: 3087 3087 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2977 2977 Size: 6148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2262 3500 830 Size: 1432 1238 3478 Fragments arranged by size: 3478 1432 1238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2184 3423 2184 Size: 1239 4909 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2258 2514 2799 3496 4631 5312 5503 Size: 219 1213 256 285 697 1135 681 191 Cuts at: 5503 826 Size: 1471 Fragments arranged by size: 1471 1213 1135 697 681 285 256 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 2289 2980 3587 513 Size: 552 939 285 691 607 3074 Fragments arranged by size: 3074 939 691 607 552 285 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4175 4192 4345 4530 4975 325 Size: 1255 2595 17 153 185 445 1498 Fragments arranged by size: 2595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4394 6106 96 Size: 274 80 33 51 1443 2417 1712 138 Fragments arranged by size: 2417 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2307 2339 6108 6140 2307 Size: 32 3769 32 2315 Fragments arranged by size: 3769 2315 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2794 1316 Size: 1478 4670 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI