(Linear) (Six-base) MAPSORT of: KIBB8519.seq Check: 7599 from: 1 to: 2859 KIBB8519.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2206 2859 Size: 2206 653 AatII G_ACGT'C Cuts at: 0 485 2859 Size: 485 2374 AccI GT'mk_AC Cuts at: 0 125 1750 2859 Size: 125 1625 1109 Fragments arranged by size: 1625 1109 125 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 2054 2761 2859 Size: 377 1677 707 98 Fragments arranged by size: 1677 707 377 98 AflII C'TTAA_G Cuts at: 0 55 541 1393 2665 2859 Size: 55 486 852 1272 194 Fragments arranged by size: 1272 852 486 194 55 AflIII A'CryG_T Cuts at: 0 1809 2859 Size: 1809 1050 AhdI GACnn_n'nnGTC Cuts at: 0 481 2859 Size: 481 2378 ApoI r'AATT_y Cuts at: 0 61 110 980 1083 1201 2859 Size: 61 49 870 103 118 1658 Fragments arranged by size: 1658 870 118 103 61 49 AseI AT'TA_AT Cuts at: 0 2006 2859 Size: 2006 853 AvaI C'yCGr_G Cuts at: 0 2645 2859 Size: 2645 214 AvrII C'CTAG_G Cuts at: 0 1545 2859 Size: 1545 1314 BamHI G'GATC_C Cuts at: 0 838 2859 Size: 838 2021 BanI G'GyrC_C Cuts at: 0 814 2859 Size: 814 2045 BanII G_rGCy'C Cuts at: 0 1567 2859 Size: 1567 1292 BbsI GAAGACnn'nnnn_ Cuts at: 0 480 722 2762 2859 Size: 480 242 2040 97 Fragments arranged by size: 2040 480 242 97 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1395 1429 2859 Size: 1395 34 1430 Fragments arranged by size: 1430 1395 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1281 2739 2859 Size: 1281 1458 120 Fragments arranged by size: 1458 1281 120 BclI T'GATC_A Cuts at: 0 2 315 429 2859 Size: 2 313 114 2430 Fragments arranged by size: 2430 313 114 2 BfrBI ATG'CAT Cuts at: 0 311 2859 Size: 311 2548 BglI GCCn_nnn'nGGC Cuts at: 0 187 2859 Size: 187 2672 Bme1580I G_kGCm'C Cuts at: 0 1900 2061 2859 Size: 1900 161 798 Fragments arranged by size: 1900 798 161 BmgBI CAC'GTC Cuts at: 0 1468 2859 Size: 1468 1391 BmrI ACTGGGnnnn_n' Cuts at: 0 1203 2264 2295 2859 Size: 1203 1061 31 564 Fragments arranged by size: 1203 1061 564 31 BmtI G_CTAG'C Cuts at: 0 770 2859 Size: 770 2089 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 523 1280 2859 Size: 484 39 757 1579 Fragments arranged by size: 1579 757 484 39 Bpu10I CC'TnA_GC Cuts at: 0 1577 2612 2859 Size: 1577 1035 247 Fragments arranged by size: 1577 1035 247 BsaI GGTCTCn'nnnn_ Cuts at: 0 1330 2680 2859 Size: 1330 1350 179 Fragments arranged by size: 1350 1330 179 BsaAI yAC'GTr Cuts at: 0 2046 2859 Size: 2046 813 BsaHI Gr'CG_yC Cuts at: 0 482 1800 2285 2859 Size: 482 1318 485 574 Fragments arranged by size: 1318 574 485 482 BsaWI w'CCGG_w Cuts at: 0 161 2859 Size: 161 2698 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 2563 2566 2779 2821 2824 2859 Size: 238 2325 3 213 42 3 35 Fragments arranged by size: 2325 238 213 42 35 3 3 BseYI C'CCAG_C Cuts at: 0 2640 2660 2859 Size: 2640 20 199 Fragments arranged by size: 2640 199 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1448 2859 Size: 1448 1411 BsiEI CG_ry'CG Cuts at: 0 14 2859 Size: 14 2845 BsmI GAATG_Cn' Cuts at: 0 87 2859 Size: 87 2772 Bsp1286I G_dGCh'C Cuts at: 0 1567 1900 2061 2859 Size: 1567 333 161 798 Fragments arranged by size: 1567 798 333 161 BspEI T'CCGG_A Cuts at: 0 161 2859 Size: 161 2698 BspHI T'CATG_A Cuts at: 0 1049 1242 2081 2859 Size: 1049 193 839 778 Fragments arranged by size: 1049 839 778 193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2206 2859 Size: 2206 653 BsrDI GCAATG_nn' Cuts at: 0 455 1413 1964 2859 Size: 455 958 551 895 Fragments arranged by size: 958 895 551 455 BstAPI GCAn_nnn'nTGC Cuts at: 0 2440 2859 Size: 2440 419 BstBI TT'CG_AA Cuts at: 0 804 1734 2859 Size: 804 930 1125 Fragments arranged by size: 1125 930 804 BstYI r'GATC_y Cuts at: 0 838 2859 Size: 838 2021 BstZ17I GTA'TAC Cuts at: 0 1751 2859 Size: 1751 1108 Bsu36I CC'TnA_GG Cuts at: 0 677 953 2859 Size: 677 276 1906 Fragments arranged by size: 1906 677 276 BtgI C'CryG_G Cuts at: 0 1454 1760 2859 Size: 1454 306 1099 Fragments arranged by size: 1454 1099 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 432 2859 Size: 432 2427 BtsI GCAGTG_nn' Cuts at: 0 918 2859 Size: 918 1941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1341 1376 2859 Size: 1341 35 1483 Fragments arranged by size: 1483 1341 35 EaeI y'GGCC_r Cuts at: 0 11 2174 2859 Size: 11 2163 685 Fragments arranged by size: 2163 685 11 EagI C'GGCC_G Cuts at: 0 11 2859 Size: 11 2848 EarI CTCTTCn'nnn_ Cuts at: 0 35 265 1676 2859 Size: 35 230 1411 1183 Fragments arranged by size: 1411 1183 230 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 377 484 523 1280 2054 2761 2859 Size: 377 107 39 757 774 707 98 Fragments arranged by size: 774 757 707 377 107 98 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 1282 1504 2374 2859 Size: 1282 222 870 485 Fragments arranged by size: 1282 870 485 222 EcoO109I rG'GnC_Cy Cuts at: 0 255 1722 2670 2859 Size: 255 1467 948 189 Fragments arranged by size: 1467 948 255 189 EcoRI G'AATT_C Cuts at: 0 1083 2859 Size: 1083 1776 EcoRV GAT'ATC Cuts at: 0 693 1413 2859 Size: 693 720 1446 Fragments arranged by size: 1446 720 693 HaeII r_GCGC'y Cuts at: 0 2189 2859 Size: 2189 670 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 467 499 720 752 868 900 2717 2749 Size: 467 32 221 32 116 32 1817 32 Cuts at: 2749 2859 Size: 110 Fragments arranged by size: 1817 467 221 116 110 32 32 32 32 MfeI C'AATT_G Cuts at: 0 374 2859 Size: 374 2485 MscI TGG'CCA Cuts at: 0 2176 2859 Size: 2176 683 MslI CAynn'nnrTG Cuts at: 0 1361 2165 2859 Size: 1361 804 694 Fragments arranged by size: 1361 804 694 MspA1I CmG'CkG Cuts at: 0 16 1534 2133 2859 Size: 16 1518 599 726 Fragments arranged by size: 1518 726 599 16 NcoI C'CATG_G Cuts at: 0 1454 1760 2859 Size: 1454 306 1099 Fragments arranged by size: 1454 1099 306 NdeI CA'TA_TG Cuts at: 0 2267 2859 Size: 2267 592 NheI G'CTAG_C Cuts at: 0 766 2859 Size: 766 2093 NsiI A_TGCA'T Cuts at: 0 313 2859 Size: 313 2546 NspI r_CATG'y Cuts at: 0 555 1813 2859 Size: 555 1258 1046 Fragments arranged by size: 1258 1046 555 PasI CC'CwG_GG Cuts at: 0 2199 2859 Size: 2199 660 PciI A'CATG_T Cuts at: 0 1809 2859 Size: 1809 1050 PflMI CCAn_nnn'nTGG Cuts at: 0 387 1193 2631 2859 Size: 387 806 1438 228 Fragments arranged by size: 1438 806 387 228 PfoI T'CCnGG_A Cuts at: 0 861 1296 2248 2859 Size: 861 435 952 611 Fragments arranged by size: 952 861 611 435 PpuMI rG'GwC_Cy Cuts at: 0 255 1722 2670 2859 Size: 255 1467 948 189 Fragments arranged by size: 1467 948 255 189 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 581 613 2859 Size: 581 32 2246 Fragments arranged by size: 2246 581 32 PstI C_TGCA'G Cuts at: 0 1019 2859 Size: 1019 1840 PvuII CAG'CTG Cuts at: 0 1534 2133 2859 Size: 1534 599 726 Fragments arranged by size: 1534 726 599 ScaI AGT'ACT Cuts at: 0 590 2859 Size: 590 2269 SfcI C'TryA_G Cuts at: 0 1015 1211 1754 2691 2859 Size: 1015 196 543 937 168 Fragments arranged by size: 1015 937 543 196 168 SmlI C'TyrA_G Cuts at: 0 55 541 1393 2665 2859 Size: 55 486 852 1272 194 Fragments arranged by size: 1272 852 486 194 55 StyI C'CwwG_G Cuts at: 0 1283 1440 1454 1545 1760 1964 2510 2859 Size: 1283 157 14 91 215 204 546 349 Fragments arranged by size: 1283 546 349 215 204 157 91 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1213 2859 Size: 1213 1646 TatI w'GTAC_w Cuts at: 0 588 2859 Size: 588 2271 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1128 1160 1962 1994 2859 Size: 1128 32 802 32 865 Fragments arranged by size: 1128 865 802 32 32 XbaI T'CTAG_A Cuts at: 0 423 2859 Size: 423 2436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 333 2859 Size: 333 2526 XmnI GAAnn'nnTTC Cuts at: 0 24 2122 2859 Size: 24 2098 737 Fragments arranged by size: 2098 737 24 ZraI GAC'GTC Cuts at: 0 483 2859 Size: 483 2376 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AhdI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I MfeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PasI PciI PflMI PfoI PpuMI PsrI PstI PvuII ScaI SfcI SmlI StyI TaqII TatI TstI XbaI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI BaeI BbeI BbvCI BglII BlpI BplI BpuEI BsaBI BsaXI BsiHKAI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI ClaI DraI DraIII DrdI EciI EcoICRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NgoMIV NotI NruI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI