################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tth111I XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 352 GTATAC GT^[AC][GT]AC AcsI 382 GAATTT [GA]^AATT[CT] AflIII 785 ACACGT A^C[GA][CT]GT AlwI 68 GGATC GGATC(4/5) AlwNI 840 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 382 GAATTT [GA]^AATT[CT] AvaI 820 CTCGAG C^[CT]CG[GA]G AvaII 849 GGACC G^G[AT]CC BbsI 290 GAAGAC GAAGAC(2/6) BglI 473 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 290 GAAGAC GAAGAC(2/6) BsaAI 786 CACGTA [CT]AC^GT[GA] BsaBI 730 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 912 GAGGAG GAGGAG(10/8) BsgI 650 GTGCAG GTGCAG(16/14) BsmAI 915 GAGAC GTCTC(1/5) BsoBI 820 CTCGAG C^[CT]CG[GA]G Bsp1407I 611 TGTACA T^GTACA BspMI 356 ACCTGC ACCTGC(4/8) BsrGI 611 TGTACA T^GTACA Bst1107I 352 GTATAC GTA^TAC BstEII 676 GGTTACC G^GT[ACGT]ACC BstPI 676 GGTTACC G^GT[ACGT]ACC BstZ17I 352 GTATAC GTA^TAC DraII 621 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 621 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 676 GGTTACC G^GT[ACGT]ACC EcoRV 722 GATATC GAT^ATC HincII 487 GTCAAC GT[CT]^[GA]AC HindII 487 GTCAAC GT[CT]^[GA]AC MaeII 787 ACGT A^CGT MamI 730 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 223 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 919 CAGCGG C[AC]G^C[GT]G NlaIV 848 GGGACC GG[ACGT]^[ACGT]CC NspI 646 GCATGT [GA]CATG^[CT] PaeR7I 820 CTCGAG C^TCGAG SfcI 419 CTGTAG C^T[GA][CT]AG SspBI 611 TGTACA T^GTACA SspI 76 AATATT AAT^ATT Tsp45I 88 GTCAC ^GT[GC]AC XbaI 282 TCTAGA T^CTAGA XcmI 465 CCATTATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 820 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbvI 195 GCAGC GCAGC(8/12) 713 GCAGC BfaI 94 CTAG C^TAG 283 CTAG BsmFI 565 GGGAC GGGAC(10/14) 848 GGGAC BstF5I 257 CATCC GGATG(2/0) 390 GGATG FokI 257 CATCC GGATG(9/13) 390 GGATG HinfI 594 GATTC G^A[ACGT]TC 932 GATTC MaeI 94 CTAG C^TAG 283 CTAG MseI 458 TTAA T^TAA 701 TTAA PflMI 260 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 839 CCAGCCTCTGG SfaNI 256 GCATC GCATC(5/9) 582 GCATC SmlI 617 CTCAAG C^T[CT][GA]AG 820 CTCGAG TfiI 594 GATTC G^A[AT]TC 932 GATTC Tru9I 458 TTAA T^TAA 701 TTAA TseI 195 GCAGC G^C[AT]GC 713 GCAGC Tsp509I 368 AATT ^AATT 383 AATT TspRI 143 CACTG CA[GC]TG[ACGT][ACGT]^ 669 CAGTG Van91I 260 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 839 CCAGCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AfaI AluI BpmI BsaJI BsiYI BslI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI TaqI TthHB8I