(Linear) (Six-base) MAPSORT of: KIBB5921.seq Check: 8754 from: 1 to: 1515 KIBB5921.orf With 182 enzymes: SgfI * March 22, 2012 15:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 312 1515 Size: 312 1203 Acc65I G'GTAC_C Cuts at: 0 266 1203 1515 Size: 266 937 312 Fragments arranged by size: 937 312 266 AflIII A'CryG_T Cuts at: 0 1472 1515 Size: 1472 43 AleI CACnn'nnGTG Cuts at: 0 1270 1515 Size: 1270 245 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 166 1515 Size: 166 1349 ApaI G_GGCC'C Cuts at: 0 46 1127 1515 Size: 46 1081 388 Fragments arranged by size: 1081 388 46 ApoI r'AATT_y Cuts at: 0 481 1515 Size: 481 1034 AvaI C'yCGr_G Cuts at: 0 45 375 782 871 1515 Size: 45 330 407 89 644 Fragments arranged by size: 644 407 330 89 45 BanI G'GyrC_C Cuts at: 0 266 295 1203 1515 Size: 266 29 908 312 Fragments arranged by size: 908 312 266 29 BanII G_rGCy'C Cuts at: 0 46 1127 1515 Size: 46 1081 388 Fragments arranged by size: 1081 388 46 BbsI GAAGACnn'nnnn_ Cuts at: 0 1508 1515 Size: 1508 7 BbvCI CC'TCA_GC Cuts at: 0 588 1515 Size: 588 927 BglI GCCn_nnn'nGGC Cuts at: 0 763 1515 Size: 763 752 Bme1580I G_kGCm'C Cuts at: 0 26 46 1127 1515 Size: 26 20 1081 388 Fragments arranged by size: 1081 388 26 20 BmgBI CAC'GTC Cuts at: 0 339 1515 Size: 339 1176 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 297 603 1515 Size: 297 306 912 Fragments arranged by size: 912 306 297 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 721 1515 Size: 712 9 794 Fragments arranged by size: 794 712 9 Bpu10I CC'TnA_GC Cuts at: 0 574 588 1515 Size: 574 14 927 Fragments arranged by size: 927 574 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 397 1420 1515 Size: 397 1023 95 Fragments arranged by size: 1023 397 95 BsaI GGTCTCn'nnnn_ Cuts at: 0 919 1515 Size: 919 596 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1219 1249 1515 Size: 1219 30 266 Fragments arranged by size: 1219 266 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 514 517 1515 Size: 514 3 998 Fragments arranged by size: 998 514 3 BseYI C'CCAG_C Cuts at: 0 389 832 970 1069 1515 Size: 389 443 138 99 446 Fragments arranged by size: 446 443 389 138 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 1515 Size: 282 1233 BsiEI CG_ry'CG Cuts at: 0 40 1515 Size: 40 1475 BsmBI CGTCTCn'nnnn_ Cuts at: 0 252 1515 Size: 252 1263 Bsp1286I G_dGCh'C Cuts at: 0 26 46 1127 1515 Size: 26 20 1081 388 Fragments arranged by size: 1081 388 26 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 254 312 1515 Size: 254 58 1203 Fragments arranged by size: 1203 254 58 BsrDI GCAATG_nn' Cuts at: 0 1161 1515 Size: 1161 354 BsrFI r'CCGG_y Cuts at: 0 13 1515 Size: 13 1502 BsrGI T'GTAC_A Cuts at: 0 1014 1515 Size: 1014 501 BstXI CCAn_nnnn'nTGG Cuts at: 0 277 1515 Size: 277 1238 BstYI r'GATC_y Cuts at: 0 254 966 1515 Size: 254 712 549 Fragments arranged by size: 712 549 254 Bsu36I CC'TnA_GG Cuts at: 0 1169 1515 Size: 1169 346 BtgI C'CryG_G Cuts at: 0 192 237 1194 1268 1515 Size: 192 45 957 74 247 Fragments arranged by size: 957 247 192 74 45 BtsI GCAGTG_nn' Cuts at: 0 887 1494 1515 Size: 887 607 21 Fragments arranged by size: 887 607 21 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1179 1214 1470 1505 1515 Size: 1179 35 256 35 10 Fragments arranged by size: 1179 256 35 35 10 EaeI y'GGCC_r Cuts at: 0 11 37 99 1277 1515 Size: 11 26 62 1178 238 Fragments arranged by size: 1178 238 62 26 11 EagI C'GGCC_G Cuts at: 0 37 1515 Size: 37 1478 EarI CTCTTCn'nnn_ Cuts at: 0 491 1303 1490 1515 Size: 491 812 187 25 Fragments arranged by size: 812 491 187 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 154 1515 Size: 154 1361 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 721 1515 Size: 712 9 794 Fragments arranged by size: 794 712 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 100 1388 1515 Size: 100 1288 127 Fragments arranged by size: 1288 127 100 EcoO109I rG'GnC_Cy Cuts at: 0 1123 1124 1515 Size: 1123 1 391 Fragments arranged by size: 1123 391 1 EcoRV GAT'ATC Cuts at: 0 1446 1515 Size: 1446 69 HaeII r_GCGC'y Cuts at: 0 20 1515 Size: 20 1495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 595 607 627 639 776 808 1036 1068 Size: 595 12 20 12 137 32 228 32 Cuts at: 1068 1515 Size: 447 Fragments arranged by size: 595 447 228 137 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 114 1515 Size: 114 1401 KpnI G_GTAC'C Cuts at: 0 270 1207 1515 Size: 270 937 308 Fragments arranged by size: 937 308 270 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 902 1061 1154 1515 Size: 902 159 93 361 Fragments arranged by size: 902 361 159 93 MslI CAynn'nnrTG Cuts at: 0 892 1270 1515 Size: 892 378 245 Fragments arranged by size: 892 378 245 MspA1I CmG'CkG Cuts at: 0 78 150 225 389 690 918 1283 1515 Size: 78 72 75 164 301 228 365 232 Fragments arranged by size: 365 301 232 228 164 78 75 72 NaeI GCC'GGC Cuts at: 0 15 1515 Size: 15 1500 NdeI CA'TA_TG Cuts at: 0 1304 1515 Size: 1304 211 NgoMIV G'CCGG_C Cuts at: 0 13 1515 Size: 13 1502 NspI r_CATG'y Cuts at: 0 384 1515 Size: 384 1131 PspOMI G'GGCC_C Cuts at: 0 42 1123 1515 Size: 42 1081 392 Fragments arranged by size: 1081 392 42 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 620 652 1515 Size: 620 32 863 Fragments arranged by size: 863 620 32 PstI C_TGCA'G Cuts at: 0 1288 1515 Size: 1288 227 PvuII CAG'CTG Cuts at: 0 225 389 690 918 1283 1515 Size: 225 164 301 228 365 232 Fragments arranged by size: 365 301 232 228 225 164 SapI GCTCTTCn'nnn_ Cuts at: 0 1303 1515 Size: 1303 212 SfcI C'TryA_G Cuts at: 0 1284 1356 1371 1515 Size: 1284 72 15 144 Fragments arranged by size: 1284 144 72 15 SmaI CCC'GGG Cuts at: 0 47 377 873 1515 Size: 47 330 496 642 Fragments arranged by size: 642 496 330 47 SmlI C'TyrA_G Cuts at: 0 412 1435 1515 Size: 412 1023 80 Fragments arranged by size: 1023 412 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1365 1515 Size: 1365 150 TatI w'GTAC_w Cuts at: 0 786 1014 1515 Size: 786 228 501 Fragments arranged by size: 786 501 228 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 933 965 1515 Size: 933 32 550 Fragments arranged by size: 933 550 32 Tth111I GACn'n_nGTC Cuts at: 0 421 1384 1515 Size: 421 963 131 Fragments arranged by size: 963 421 131 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 846 1448 1515 Size: 846 602 67 Fragments arranged by size: 846 602 67 XmaI C'CCGG_G Cuts at: 0 45 375 871 1515 Size: 45 330 496 644 Fragments arranged by size: 644 496 330 45 XmnI GAAnn'nnTTC Cuts at: 0 1474 1515 Size: 1474 41 Enzymes that do cut: AarI Acc65I AflIII AleI AlfI ApaI ApoI AvaI BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KpnI MmeI MslI MspA1I NaeI NdeI NgoMIV NspI PspOMI PsrI PstI PvuII SapI SfcI SmaI SmlI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AgeI AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EcoICRI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MscI NarI NcoI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI XbaI XhoI ZraI