(Linear) (Six-base) MAPSORT of: KIEE2613.seq Check: 5456 from: 1 to: 1230 KIEE2613.orf With 182 enzymes: SgfI * March 25, 2016 09:08 .. Acc65I G'GTAC_C Cuts at: 0 1100 1230 Size: 1100 130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 928 1230 Size: 928 302 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 52 84 1230 Size: 52 32 1146 Fragments arranged by size: 1146 52 32 AlwNI CAG_nnn'CTG Cuts at: 0 90 681 1097 1230 Size: 90 591 416 133 Fragments arranged by size: 591 416 133 90 AvaI C'yCGr_G Cuts at: 0 257 533 1230 Size: 257 276 697 Fragments arranged by size: 697 276 257 BamHI G'GATC_C Cuts at: 0 101 1230 Size: 101 1129 BanI G'GyrC_C Cuts at: 0 34 320 1100 1143 1230 Size: 34 286 780 43 87 Fragments arranged by size: 780 286 87 43 34 BanII G_rGCy'C Cuts at: 0 17 161 667 839 1141 1230 Size: 17 144 506 172 302 89 Fragments arranged by size: 506 302 172 144 89 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 420 925 1230 Size: 420 505 305 Fragments arranged by size: 505 420 305 BbvCI CC'TCA_GC Cuts at: 0 1160 1166 1230 Size: 1160 6 64 Fragments arranged by size: 1160 64 6 BfrBI ATG'CAT Cuts at: 0 269 1230 Size: 269 961 BglI GCCn_nnn'nGGC Cuts at: 0 648 899 1230 Size: 648 251 331 Fragments arranged by size: 648 331 251 BglII A'GATC_T Cuts at: 0 539 1230 Size: 539 691 BlpI GC'TnA_GC Cuts at: 0 553 578 1230 Size: 553 25 652 Fragments arranged by size: 652 553 25 Bme1580I G_kGCm'C Cuts at: 0 99 470 1148 1230 Size: 99 371 678 82 Fragments arranged by size: 678 371 99 82 BmrI ACTGGGnnnn_n' Cuts at: 0 141 1230 Size: 141 1089 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 177 1230 Size: 177 1053 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 172 181 214 655 853 870 1009 Size: 147 25 9 33 441 198 17 139 Cuts at: 1009 1230 Size: 221 Fragments arranged by size: 441 221 198 147 139 33 25 17 9 Bpu10I CC'TnA_GC Cuts at: 0 1160 1166 1230 Size: 1160 6 64 Fragments arranged by size: 1160 64 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1192 1230 Size: 1192 38 BsaAI yAC'GTr Cuts at: 0 243 1230 Size: 243 987 BsaBI GATnn'nnATC Cuts at: 0 515 1230 Size: 515 715 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 430 433 436 941 974 1230 Size: 169 261 3 3 505 33 256 Fragments arranged by size: 505 261 256 169 33 3 3 BseYI C'CCAG_C Cuts at: 0 125 211 451 1230 Size: 125 86 240 779 Fragments arranged by size: 779 240 125 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 765 1230 Size: 765 465 BsiHKAI G_wGCw'C Cuts at: 0 161 1230 Size: 161 1069 BsmBI CGTCTCn'nnnn_ Cuts at: 0 875 1230 Size: 875 355 Bsp1286I G_dGCh'C Cuts at: 0 17 99 161 470 667 839 1141 1148 Size: 17 82 62 309 197 172 302 7 Cuts at: 1148 1230 Size: 82 Fragments arranged by size: 309 302 197 172 82 82 62 17 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1051 1230 Size: 1051 179 BsrDI GCAATG_nn' Cuts at: 0 179 958 1177 1230 Size: 179 779 219 53 Fragments arranged by size: 779 219 179 53 BsrFI r'CCGG_y Cuts at: 0 49 1230 Size: 49 1181 BsrGI T'GTAC_A Cuts at: 0 302 1230 Size: 302 928 BstAPI GCAn_nnn'nTGC Cuts at: 0 181 1230 Size: 181 1049 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 384 894 1111 1230 Size: 171 213 510 217 119 Fragments arranged by size: 510 217 213 171 119 BstYI r'GATC_y Cuts at: 0 101 539 1230 Size: 101 438 691 Fragments arranged by size: 691 438 101 BtgI C'CryG_G Cuts at: 0 506 1230 Size: 506 724 BtsI GCAGTG_nn' Cuts at: 0 729 1079 1230 Size: 729 350 151 Fragments arranged by size: 729 350 151 DrdI GACnn_nn'nnGTC Cuts at: 0 184 1230 Size: 184 1046 EaeI y'GGCC_r Cuts at: 0 386 461 749 1230 Size: 386 75 288 481 Fragments arranged by size: 481 386 288 75 EarI CTCTTCn'nnn_ Cuts at: 0 998 1230 Size: 998 232 EcoICRI GAG'CTC Cuts at: 0 159 1230 Size: 159 1071 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 172 181 214 655 853 870 1009 Size: 147 25 9 33 441 198 17 139 Cuts at: 1009 1230 Size: 221 Fragments arranged by size: 441 221 198 147 139 33 25 17 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 486 1230 Size: 486 744 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 279 311 1230 Size: 279 32 919 Fragments arranged by size: 919 279 32 KpnI G_GTAC'C Cuts at: 0 1104 1230 Size: 1104 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 542 1230 Size: 542 688 MscI TGG'CCA Cuts at: 0 388 1230 Size: 388 842 MslI CAynn'nnrTG Cuts at: 0 382 1109 1230 Size: 382 727 121 Fragments arranged by size: 727 382 121 MspA1I CmG'CkG Cuts at: 0 678 890 1230 Size: 678 212 340 Fragments arranged by size: 678 340 212 NaeI GCC'GGC Cuts at: 0 51 1230 Size: 51 1179 NcoI C'CATG_G Cuts at: 0 506 1230 Size: 506 724 NdeI CA'TA_TG Cuts at: 0 714 1230 Size: 714 516 NgoMIV G'CCGG_C Cuts at: 0 49 1230 Size: 49 1181 NsiI A_TGCA'T Cuts at: 0 271 1230 Size: 271 959 NspI r_CATG'y Cuts at: 0 269 349 1230 Size: 269 80 881 Fragments arranged by size: 881 269 80 PasI CC'CwG_GG Cuts at: 0 660 1230 Size: 660 570 PflMI CCAn_nnn'nTGG Cuts at: 0 512 681 1097 1230 Size: 512 169 416 133 Fragments arranged by size: 512 416 169 133 PfoI T'CCnGG_A Cuts at: 0 56 830 1230 Size: 56 774 400 Fragments arranged by size: 774 400 56 PvuII CAG'CTG Cuts at: 0 890 1230 Size: 890 340 SacI G_AGCT'C Cuts at: 0 161 1230 Size: 161 1069 SfcI C'TryA_G Cuts at: 0 144 1230 Size: 144 1086 SmaI CCC'GGG Cuts at: 0 259 1230 Size: 259 971 SmlI C'TyrA_G Cuts at: 0 1207 1230 Size: 1207 23 SnaBI TAC'GTA Cuts at: 0 243 1230 Size: 243 987 SphI G_CATG'C Cuts at: 0 269 1230 Size: 269 961 StyI C'CwwG_G Cuts at: 0 482 506 807 1132 1230 Size: 482 24 301 325 98 Fragments arranged by size: 482 325 301 98 24 TatI w'GTAC_w Cuts at: 0 302 1230 Size: 302 928 XmaI C'CCGG_G Cuts at: 0 257 1230 Size: 257 973 Enzymes that do cut: Acc65I AlfI AloI AlwNI AvaI BamHI BanI BanII BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI PvuII SacI SfcI SmaI SmlI SnaBI SphI StyI TatI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BmgBI BmtI BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI NarI NheI NotI NruI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI