################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AfaI 717 GTAC GT^AC AlwNI 919 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AscI 1024 GGCGCGCC GG^CGCGCC AspHI 175 GTGCTC G[AT]GC[AT]^C BamHI 327 GGATCC G^GATCC BglII 827 AGATCT A^GATCT BsgI 535 GTGCAG GTGCAG(16/14) BsiHKAI 175 GTGCTC G[AT]GC[AT]^C BsmAI 15 GTCTC GTCTC(1/5) BssHII 1025 GCGCGC G^CGCGC BssSI 853 CTCGTG CACGAG(-5/-1) BstAPI 918 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 353 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 277 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 38 CCAAGG C^C[AT][AT]GG MaeIII 247 GTGAC ^GT[ACGT]AC NaeI 258 GCCGGC GCC^GGC NgoMIV 258 GCCGGC G^CCGGC NlaIII 816 CATG CATG^ NotI 427 GCGGCCGC GC^GGCCGC PflMI 1073 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1056 CAGCTG CAG^CTG RsaI 717 GTAC GT^AC SexAI 704 ACCTGGT A^CC[AT]GGT SmaI 615 CCCGGG CCC^GGG StuI 683 AGGCCT AGG^CCT StyI 38 CCAAGG C^C[AT][AT]GG Tsp45I 247 GTGAC ^GT[GC]AC Van91I 1073 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 615 CCCGGG C^CCGGG XmaI 615 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 328 GATCC GGATC(4/5) 327 GGATC Aor51HI 872 AGCGCT AGC^GCT 884 AGCGCT ApaI 270 GGGCCC GGGCC^C 1008 GGGCCC BstF5I 263 CATCC GGATG(2/0) 900 CATCC BstYI 327 GGATCC [GA]^GATC[CT] 827 AGATCT DpnII 328 GATC ^GATC 828 GATC DraII 270 GGGCCCC [GA]G^G[ACGT]CC[CT] 1007 GGGGCCC Eco47III 872 AGCGCT AGC^GCT 884 AGCGCT EcoO109I 270 GGGCCCC [GA]G^G[ACGT]CC[CT] 1007 GGGGCCC FokI 263 CATCC GGATG(9/13) 900 CATCC HinfI 158 GACTC G^A[ACGT]TC 733 GAGTC MboI 328 GATC ^GATC 828 GATC MflI 327 GGATCC [GA]^GATC[CT] 827 AGATCT NdeII 328 GATC ^GATC 828 GATC PleI 158 GACTC GAGTC(4/5) 733 GAGTC PstI 664 CTGCAG CTGCA^G 772 CTGCAG Sau3AI 328 GATC ^GATC 828 GATC SfaNI 921 GATGC GCATC(5/9) 262 GCATC SfcI 664 CTGCAG C^T[GA][CT]AG 772 CTGCAG SgrAI 500 CGCCGGTG C[GA]^CCGG[CT]G 546 CACCGGCG XhoII 327 GGATCC [GA]^GATC[CT] 827 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BlpI BmyI Bpu1102I BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI KspI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV SacII Sau96I ScrFI SfoI SmlI TseI TspRI