################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI ItaI KasI KpnI KspI MaeI MamI MfeI MluI MseI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 65 TCCGGA T^CCGGA AluI 150 AGCT AG^CT AlwNI 188 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 44 GGACC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanI 199 GGTGCC G^G[CT][GA]CC BsaWI 65 TCCGGA [AT]^CCGG[AT] BseAI 65 TCCGGA T^CCGGA BsmAI 144 GAGAC GTCTC(1/5) BsmBI 144 GAGACG CGTCTC(1/5) BsmFI 43 GGGAC GGGAC(10/14) BspEI 65 TCCGGA T^CCGGA BssKI 203 CCTGG ^CC[ACGT]GG BstNI 203 CCTGG CC^[AT]GG BstYI 158 GGATCT [GA]^GATC[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 141 GAAGAG CTCTTC(1/4) EcoRII 203 CCTGG ^CC[AT]GG FauI 47 CCCGC CCCGC(4/6) HapII 66 CCGG C^CGG HpaII 66 CCGG C^CGG HphI 75 GGTGA GGTGA(8/7) Ksp632I 141 GAAGAG CTCTTC(1/4) MaeIII 229 GTCAC ^GT[ACGT]AC MflI 158 GGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MroI 65 TCCGGA T^CCGGA MscI 2 TGGCCA TGG^CCA MspA1I 224 CCGCTG C[AC]G^C[GT]G MspI 66 CCGG C^CGG MvaI 203 CCTGG CC^[AT]GG MwoI 193 GCTGGCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 180 CATG CATG^ PpuMI 43 GGGACCC [GA]G^G[AT]CC[CT] ScrFI 203 CCTGG CC^[ACGT]GG Tsp45I 229 GTCAC ^GT[GC]AC Tsp509I 84 AATT ^AATT XhoII 158 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwI 26 GGATC GGATC(4/5) 158 GGATC BbsI 183 GAAGAC GAAGAC(2/6) 251 GAAGAC BpuAI 183 GAAGAC GAAGAC(2/6) 251 GAAGAC Cfr13I 44 GGACC G^G[ACGT]CC 56 GGGCC DpnII 27 GATC ^GATC 159 GATC DraII 43 GGGACCC [GA]G^G[ACGT]CC[CT] 55 GGGGCCT EcoO109I 43 GGGACCC [GA]G^G[ACGT]CC[CT] 55 GGGGCCT HaeIII 3 GGCC GG^CC 57 GGCC MaeII 9 ACGT A^CGT 208 ACGT MboI 27 GATC ^GATC 159 GATC MnlI 59 CCTC CCTC(7/6) 63 CCTC NdeII 27 GATC ^GATC 159 GATC Sau3AI 27 GATC ^GATC 159 GATC Sau96I 44 GGACC G^G[ACGT]CC 56 GGGCC TspRI 166 CACTG CA[GC]TG[ACGT][ACGT]^ 188 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AciI AfaI BsiYI BslI Cac8I MboII NlaIV RsaI