(Circular) (Six-base) MAPSORT of: pFN21AE5149.seq Check: 9322 from: 1 to: 4981 pFN21AE5149 4981 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2791 278 Size: 53 83 186 936 1255 2468 Fragments arranged by size: 2468 1255 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2324 1937 Size: 387 4594 Acc65I G'GTAC_C Cuts at: 2302 2302 Size: 4981 AclI AA'CG_TT Cuts at: 1563 3108 3481 1563 Size: 1545 373 3063 Fragments arranged by size: 3063 1545 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3044 4428 4696 1632 Size: 650 762 1384 268 1917 Fragments arranged by size: 1917 1384 762 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4784 AflIII A'CryG_T Cuts at: 1203 3880 4857 1203 Size: 2677 977 1327 Fragments arranged by size: 2677 1327 977 AgeI A'CCGG_T Cuts at: 3812 3812 Size: 4981 AhdI GACnn_n'nnGTC Cuts at: 3710 3710 Size: 4981 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4981 AlwNI CAG_nnn'CTG Cuts at: 2198 2724 4296 2198 Size: 526 1572 2883 Fragments arranged by size: 2883 1572 526 ApaLI G'TGCA_C Cuts at: 3038 4194 3038 Size: 1156 3825 ApoI r'AATT_y Cuts at: 1493 2290 2516 2668 4777 1493 Size: 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 226 152 AseI AT'TA_AT Cuts at: 160 1032 3535 160 Size: 872 2503 1606 Fragments arranged by size: 2503 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4981 AvaI C'yCGr_G Cuts at: 1870 1943 2306 1870 Size: 73 363 4545 Fragments arranged by size: 4545 363 73 BamHI G'GATC_C Cuts at: 2311 2678 2311 Size: 367 4614 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 2302 3757 618 Size: 325 217 627 417 98 1455 1842 Fragments arranged by size: 1842 1455 627 417 325 217 98 BanII G_rGCy'C Cuts at: 1391 2300 1391 Size: 909 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2195 2263 928 Size: 971 296 68 3646 Fragments arranged by size: 3646 971 296 68 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3172 3206 1903 Size: 34 1235 34 3678 Fragments arranged by size: 3678 1235 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2873 4083 2873 Size: 1210 3771 BclI T'GATC_A Cuts at: 1548 1548 Size: 4981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3592 136 Size: 107 122 71 631 605 1920 1525 Fragments arranged by size: 1920 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4976 4976 Size: 4981 BlpI GC'TnA_GC Cuts at: 2398 2398 Size: 4981 Bme1580I G_kGCm'C Cuts at: 1790 3042 4198 1790 Size: 1252 1156 2573 Fragments arranged by size: 2573 1252 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4981 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2712 3670 442 Size: 416 53 83 399 24 1295 958 1753 Fragments arranged by size: 1753 1295 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4981 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3641 1239 Size: 2402 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3106 3971 4269 3106 Size: 865 298 3818 Fragments arranged by size: 3818 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3644 882 Size: 617 2145 2219 Fragments arranged by size: 2219 2145 617 BsaAI yAC'GTr Cuts at: 493 1204 2772 493 Size: 711 1568 2702 Fragments arranged by size: 2702 1568 711 BsaBI GATnn'nnATC Cuts at: 4975 4975 Size: 4981 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2788 3170 3805 275 Size: 53 83 186 936 1255 382 635 1451 Fragments arranged by size: 1451 1255 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2070 3414 3812 4086 4233 4644 2070 Size: 1344 398 274 147 411 2407 Fragments arranged by size: 2407 1344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4756 Fragments arranged by size: 4756 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4981 BseYI C'CCAG_C Cuts at: 4184 4680 4184 Size: 496 4485 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2431 3192 4220 1540 Size: 275 186 430 761 1028 2301 Fragments arranged by size: 2301 1028 761 430 275 186 BsiHKAI G_wGCw'C Cuts at: 3042 3127 4198 3042 Size: 85 1071 3825 Fragments arranged by size: 3825 1071 85 BsmI GAATG_Cn' Cuts at: 2492 2585 2492 Size: 93 4888 BsmBI CGTCTCn'nnnn_ Cuts at: 2143 2143 Size: 4981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2300 3042 3127 4198 1391 Size: 399 510 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 399 85 BspEI T'CCGG_A Cuts at: 2070 4644 2070 Size: 2574 2407 BspHI T'CATG_A Cuts at: 2870 4600 2870 Size: 1730 3251 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2336 844 Size: 1492 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2868 1394 Size: 429 1045 3507 Fragments arranged by size: 3507 1045 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3476 3650 65 Size: 1183 2228 174 1396 Fragments arranged by size: 2228 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3625 3812 1700 Size: 110 1815 187 2869 Fragments arranged by size: 2869 1815 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4981 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4981 BssSI C'ACGA_G Cuts at: 3041 4053 3041 Size: 1012 3969 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4981 BstBI TT'CG_AA Cuts at: 3785 3785 Size: 4981 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4981 BstYI r'GATC_y Cuts at: 1972 2163 2311 2678 3063 3080 4532 4618 4630 Size: 191 148 367 385 17 1452 86 12 Cuts at: 4630 4976 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 191 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3490 Fragments arranged by size: 3490 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4242 Fragments arranged by size: 4242 683 56 BtsI GCAGTG_nn' Cuts at: 2497 3291 3311 2497 Size: 794 20 4167 Fragments arranged by size: 4167 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4981 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4946 DraI TTT'AAA Cuts at: 2285 2285 Size: 4981 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3988 809 Size: 507 2672 1802 Fragments arranged by size: 2672 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2428 3317 Size: 54 1425 50 275 18 177 421 889 Cuts at: 3317 4946 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 421 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2428 1537 Size: 275 616 4090 Fragments arranged by size: 4090 616 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 2911 1353 Size: 788 770 3423 Fragments arranged by size: 3423 788 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2236 3568 3942 4088 387 Size: 1064 138 647 1332 374 146 1280 Fragments arranged by size: 1332 1280 1064 647 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 3044 3641 4428 4696 1239 Size: 393 650 762 597 787 268 1524 Fragments arranged by size: 1524 787 762 650 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2049 2061 2049 Size: 12 4969 EcoRI G'AATT_C Cuts at: 2290 2668 2290 Size: 378 4603 FspI TGC'GCA Cuts at: 3487 3487 Size: 4981 HaeII r_GCGC'y Cuts at: 1678 4128 1678 Size: 2450 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3622 3654 3696 3728 1373 Size: 32 135 32 2050 32 42 32 2626 Fragments arranged by size: 2626 2050 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2325 2571 669 Size: 988 281 387 246 3079 Fragments arranged by size: 3079 988 387 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4981 HpaI GTT'AAC Cuts at: 2571 2571 Size: 4981 KpnI G_GTAC'C Cuts at: 2306 2306 Size: 4981 MfeI C'AATT_G Cuts at: 2580 2580 Size: 4981 MluI A'CGCG_T Cuts at: 4857 4857 Size: 4981 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4095 4279 1305 Size: 2790 184 2007 Fragments arranged by size: 2790 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 2982 Fragments arranged by size: 2982 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3298 3457 518 Size: 648 1773 359 159 2042 Fragments arranged by size: 2042 1773 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2397 3074 4222 4467 1606 Size: 625 166 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 625 245 166 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3490 Fragments arranged by size: 3490 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4981 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4981 NotI GC'GGCC_GC Cuts at: 2428 2428 Size: 4981 NspI r_CATG'y Cuts at: 1132 1229 2339 3884 1132 Size: 97 1110 1545 2229 Fragments arranged by size: 2229 1545 1110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4981 PciI A'CATG_T Cuts at: 3880 3880 Size: 4981 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4981 PmeI GTTT'AAAC Cuts at: 2285 2285 Size: 4981 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3050 3082 4563 4595 3050 Size: 32 1481 32 3436 Fragments arranged by size: 3436 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2049 2049 Size: 4981 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4981 PsiI TTA'TAA Cuts at: 2551 2551 Size: 4981 PstI C_TGCA'G Cuts at: 830 2333 830 Size: 1503 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4981 SalI G'TCGA_C Cuts at: 1936 2323 1936 Size: 387 4594 SanDI GG'GwC_CC Cuts at: 2049 2049 Size: 4981 SbfI CC_TGCA'GG Cuts at: 2333 2333 Size: 4981 ScaI AGT'ACT Cuts at: 3229 4941 3229 Size: 1712 3269 SexAI A'CCwGG_T Cuts at: 2277 2277 Size: 4981 SfcI C'TryA_G Cuts at: 826 1045 2329 3464 4145 4336 826 Size: 219 1284 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4981 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4981 SmaI CCC'GGG Cuts at: 1872 2308 1872 Size: 436 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3085 3986 4248 820 Size: 197 926 1142 901 262 1553 Fragments arranged by size: 1553 1142 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2772 493 Size: 2279 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4981 SphI G_CATG'C Cuts at: 1132 2339 1132 Size: 1207 3774 SspI AAT'ATT Cuts at: 5 52 2905 5 Size: 47 2853 2081 Fragments arranged by size: 2853 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4981 StyI C'CwwG_G Cuts at: 513 1065 2004 2420 513 Size: 552 939 416 3074 Fragments arranged by size: 3074 939 552 416 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3008 3025 3178 3363 3808 325 Size: 1255 1428 17 153 185 445 1498 Fragments arranged by size: 1498 1428 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3227 4939 Size: 274 80 33 51 1443 135 1115 1712 Cuts at: 4939 96 Size: 138 Fragments arranged by size: 1712 1443 1115 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2162 2194 4941 4973 2162 Size: 32 2747 32 2170 Fragments arranged by size: 2747 2170 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4981 XbaI T'CTAG_A Cuts at: 2317 2317 Size: 4981 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4981 XmaI C'CCGG_G Cuts at: 1870 2306 1870 Size: 436 4545 XmnI GAAnn'nnTTC Cuts at: 3110 3110 Size: 4981 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2789 276 Size: 53 83 186 936 1255 2468 Fragments arranged by size: 2468 1255 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI