(Linear) (Six-base) MAPSORT of: KIEE2068.seq Check: 4692 from: 1 to: 2181 KIEE2068.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1015 2181 Size: 1015 1166 AccI GT'mk_AC Cuts at: 0 528 2181 Size: 528 1653 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1064 1124 2181 Size: 1064 60 1057 Fragments arranged by size: 1064 1057 60 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1156 2054 2181 Size: 1156 898 127 Fragments arranged by size: 1156 898 127 AlwNI CAG_nnn'CTG Cuts at: 0 1129 1759 2181 Size: 1129 630 422 Fragments arranged by size: 1129 630 422 ApoI r'AATT_y Cuts at: 0 1349 2181 Size: 1349 832 BanI G'GyrC_C Cuts at: 0 685 1230 2149 2181 Size: 685 545 919 32 Fragments arranged by size: 919 685 545 32 BanII G_rGCy'C Cuts at: 0 1731 1809 2012 2181 Size: 1731 78 203 169 Fragments arranged by size: 1731 203 169 78 BbsI GAAGACnn'nnnn_ Cuts at: 0 488 1104 1859 2181 Size: 488 616 755 322 Fragments arranged by size: 755 616 488 322 BbvCI CC'TCA_GC Cuts at: 0 2060 2181 Size: 2060 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 163 197 2181 Size: 163 34 1984 Fragments arranged by size: 1984 163 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2 2181 Size: 2 2179 BfrBI ATG'CAT Cuts at: 0 831 2181 Size: 831 1350 BlpI GC'TnA_GC Cuts at: 0 1808 2181 Size: 1808 373 Bme1580I G_kGCm'C Cuts at: 0 690 2154 2181 Size: 690 1464 27 Fragments arranged by size: 1464 690 27 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 873 2181 Size: 873 1308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 821 2181 Size: 821 1360 Bpu10I CC'TnA_GC Cuts at: 0 2060 2181 Size: 2060 121 BsaAI yAC'GTr Cuts at: 0 826 2181 Size: 826 1355 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1421 2181 Size: 1421 760 BsiEI CG_ry'CG Cuts at: 0 143 2181 Size: 143 2038 BsiHKAI G_wGCw'C Cuts at: 0 1809 1875 2012 2181 Size: 1809 66 137 169 Fragments arranged by size: 1809 169 137 66 Bsp1286I G_dGCh'C Cuts at: 0 690 1731 1809 1875 2012 2154 2181 Size: 690 1041 78 66 137 142 27 Fragments arranged by size: 1041 690 142 137 78 66 27 BspHI T'CATG_A Cuts at: 0 195 515 2181 Size: 195 320 1666 Fragments arranged by size: 1666 320 195 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1015 2181 Size: 1015 1166 BsrDI GCAATG_nn' Cuts at: 0 1361 1484 2181 Size: 1361 123 697 Fragments arranged by size: 1361 697 123 BstAPI GCAn_nnn'nTGC Cuts at: 0 230 1021 1468 2181 Size: 230 791 447 713 Fragments arranged by size: 791 713 447 230 BstBI TT'CG_AA Cuts at: 0 63 1254 2181 Size: 63 1191 927 Fragments arranged by size: 1191 927 63 BstYI r'GATC_y Cuts at: 0 176 854 2181 Size: 176 678 1327 Fragments arranged by size: 1327 678 176 BstZ17I GTA'TAC Cuts at: 0 529 2181 Size: 529 1652 Bsu36I CC'TnA_GG Cuts at: 0 257 2181 Size: 257 1924 BtsI GCAGTG_nn' Cuts at: 0 1118 1832 2181 Size: 1118 714 349 Fragments arranged by size: 1118 714 349 EaeI y'GGCC_r Cuts at: 0 2120 2181 Size: 2120 61 EarI CTCTTCn'nnn_ Cuts at: 0 842 1100 1178 1904 2181 Size: 842 258 78 726 277 Fragments arranged by size: 842 726 277 258 78 EcoICRI GAG'CTC Cuts at: 0 1807 2010 2181 Size: 1807 203 171 Fragments arranged by size: 1807 203 171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 821 1064 1124 2181 Size: 821 243 60 1057 Fragments arranged by size: 1057 821 243 60 EcoO109I rG'GnC_Cy Cuts at: 0 1392 2181 Size: 1392 789 EcoRV GAT'ATC Cuts at: 0 672 2181 Size: 672 1509 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1087 1306 2181 Size: 1087 219 875 Fragments arranged by size: 1087 875 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 952 984 1545 1577 1646 1678 Size: 100 32 820 32 561 32 69 32 Cuts at: 1678 2155 2181 Size: 477 26 Fragments arranged by size: 820 561 477 100 69 32 32 32 32 26 HincII GTy'rAC Cuts at: 0 1791 2181 Size: 1791 390 HindIII A'AGCT_T Cuts at: 0 152 2181 Size: 152 2029 MfeI C'AATT_G Cuts at: 0 1019 1169 2181 Size: 1019 150 1012 Fragments arranged by size: 1019 1012 150 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 277 750 2181 Size: 277 473 1431 Fragments arranged by size: 1431 473 277 MscI TGG'CCA Cuts at: 0 2122 2181 Size: 2122 59 MslI CAynn'nnrTG Cuts at: 0 1715 1718 2175 2181 Size: 1715 3 457 6 Fragments arranged by size: 1715 457 6 3 MspA1I CmG'CkG Cuts at: 0 1474 2181 Size: 1474 707 NdeI CA'TA_TG Cuts at: 0 1323 1785 1905 2176 2181 Size: 1323 462 120 271 5 Fragments arranged by size: 1323 462 271 120 5 NsiI A_TGCA'T Cuts at: 0 833 2181 Size: 833 1348 NspI r_CATG'y Cuts at: 0 174 2181 Size: 174 2007 PpuMI rG'GwC_Cy Cuts at: 0 1392 2181 Size: 1392 789 PsiI TTA'TAA Cuts at: 0 1867 2181 Size: 1867 314 PstI C_TGCA'G Cuts at: 0 226 419 1125 2181 Size: 226 193 706 1056 Fragments arranged by size: 1056 706 226 193 PvuII CAG'CTG Cuts at: 0 1474 2181 Size: 1474 707 SacI G_AGCT'C Cuts at: 0 1809 2012 2181 Size: 1809 203 169 Fragments arranged by size: 1809 203 169 SfcI C'TryA_G Cuts at: 0 222 415 680 1121 2181 Size: 222 193 265 441 1060 Fragments arranged by size: 1060 441 265 222 193 SpeI A'CTAG_T Cuts at: 0 1507 2181 Size: 1507 674 SphI G_CATG'C Cuts at: 0 174 2181 Size: 174 2007 SspI AAT'ATT Cuts at: 0 780 2181 Size: 780 1401 StuI AGG'CCT Cuts at: 0 25 35 2181 Size: 25 10 2146 Fragments arranged by size: 2146 25 10 StyI C'CwwG_G Cuts at: 0 724 1581 1625 2181 Size: 724 857 44 556 Fragments arranged by size: 857 724 556 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 386 2181 Size: 386 1795 TatI w'GTAC_w Cuts at: 0 535 2181 Size: 535 1646 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1494 1526 2181 Size: 1494 32 655 Fragments arranged by size: 1494 655 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 756 1450 2181 Size: 756 694 731 Fragments arranged by size: 756 731 694 XmnI GAAnn'nnTTC Cuts at: 0 1607 2181 Size: 1607 574 Enzymes that do cut: AarI AccI AcuI AlfI AlwNI ApoI BanI BanII BbsI BbvCI BcgI BciVI BfrBI BlpI Bme1580I BplI BpmI Bpu10I BsaAI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstAPI BstBI BstYI BstZ17I Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NdeI NsiI NspI PpuMI PsiI PstI PvuII SacI SfcI SpeI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BglI BglII BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI