Restriction map for
pFN21AB6904
(Linear) (Six-base) MAPSORT of: KIBB6904.seq Check: 26 from: 1 to: 669
KIBB6904.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 669
Size: 266 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 669
Size: 127 542
AlwNI CAG_nnn'CTG
Cuts at: 0 56 478 669
Size: 56 422 191
Fragments arranged by size:
422 191 56
BamHI G'GATC_C
Cuts at: 0 494 669
Size: 494 175
BanI G'GyrC_C
Cuts at: 0 313 669
Size: 313 356
BanII G_rGCy'C
Cuts at: 0 398 669
Size: 398 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 669
Size: 90 579
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 494 528 669
Size: 494 34 141
Fragments arranged by size:
494 141 34
BfrBI ATG'CAT
Cuts at: 0 181 669
Size: 181 488
BlpI GC'TnA_GC
Cuts at: 0 32 669
Size: 32 637
Bme1580I G_kGCm'C
Cuts at: 0 316 472 669
Size: 316 156 197
Fragments arranged by size:
316 197 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 303 669
Size: 170 133 366
Fragments arranged by size:
366 170 133
BsaAI yAC'GTr
Cuts at: 0 117 669
Size: 117 552
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 669
Size: 385 30 254
Fragments arranged by size:
385 254 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 529 669
Size: 478 51 140
Fragments arranged by size:
478 140 51
BseYI C'CCAG_C
Cuts at: 0 122 669
Size: 122 547
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 291 669
Size: 48 243 378
Fragments arranged by size:
378 243 48
BsiHKAI G_wGCw'C
Cuts at: 0 398 669
Size: 398 271
Bsp1286I G_dGCh'C
Cuts at: 0 316 398 472 669
Size: 316 82 74 197
Fragments arranged by size:
316 197 82 74
BsrDI GCAATG_nn'
Cuts at: 0 116 669
Size: 116 553
BstYI r'GATC_y
Cuts at: 0 494 669
Size: 494 175
BtgI C'CryG_G
Cuts at: 0 362 669
Size: 362 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 141 669
Size: 141 528
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 98 133 669
Size: 98 35 536
Fragments arranged by size:
536 98 35
EaeI y'GGCC_r
Cuts at: 0 140 669
Size: 140 529
EarI CTCTTCn'nnn_
Cuts at: 0 91 669
Size: 91 578
EcoICRI GAG'CTC
Cuts at: 0 396 669
Size: 396 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 266 303 669
Size: 170 96 37 366
Fragments arranged by size:
366 170 96 37
EcoO109I rG'GnC_Cy
Cuts at: 0 53 669
Size: 53 616
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 365 397 669
Size: 166 32 167 32 272
Fragments arranged by size:
272 167 166 32 32
HincII GTy'rAC
Cuts at: 0 284 669
Size: 284 385
MscI TGG'CCA
Cuts at: 0 142 669
Size: 142 527
MspA1I CmG'CkG
Cuts at: 0 21 36 547 669
Size: 21 15 511 122
Fragments arranged by size:
511 122 21 15
NcoI C'CATG_G
Cuts at: 0 362 669
Size: 362 307
NsiI A_TGCA'T
Cuts at: 0 183 669
Size: 183 486
PflMI CCAn_nnn'nTGG
Cuts at: 0 56 669
Size: 56 613
PmlI CAC'GTG
Cuts at: 0 117 669
Size: 117 552
PpuMI rG'GwC_Cy
Cuts at: 0 53 669
Size: 53 616
PshAI GACnn'nnGTC
Cuts at: 0 247 669
Size: 247 422
PvuII CAG'CTG
Cuts at: 0 36 547 669
Size: 36 511 122
Fragments arranged by size:
511 122 36
SacI G_AGCT'C
Cuts at: 0 398 669
Size: 398 271
StyI C'CwwG_G
Cuts at: 0 9 348 362 669
Size: 9 339 14 307
Fragments arranged by size:
339 307 14 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 669
Size: 317 32 320
Fragments arranged by size:
320 317 32
Enzymes that do cut:
AcuI AlfI AlwNI BamHI BanI BanII BbsI BcgI
BfrBI BlpI Bme1580I BpmI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BsrDI BstYI BtgI BtgZI CspCI
EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII MscI
MspA1I NcoI NsiI PflMI PmlI PpuMI PshAI PvuII
SacI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI
BclI BglI BglII BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI