(Circular) (Six-base) MAPSORT of: pFN21AE1716.seq Check: 724 from: 1 to: 5365 pFN21AE1716 5365 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3175 278 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2708 1937 Size: 771 4594 Acc65I G'GTAC_C Cuts at: 2686 2686 Size: 5365 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 3428 4812 5080 1632 Size: 584 1212 1384 268 1917 Fragments arranged by size: 1917 1384 1212 584 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 4264 5241 1203 Size: 3061 977 1327 Fragments arranged by size: 3061 1327 977 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 3970 AlwNI CAG_nnn'CTG Cuts at: 3108 4680 3108 Size: 1572 3793 ApaI G_GGCC'C Cuts at: 2607 2607 Size: 5365 ApaLI G'TGCA_C Cuts at: 3422 4578 3422 Size: 1156 4209 ApoI r'AATT_y Cuts at: 1493 2305 2674 2900 3052 5161 1493 Size: 812 369 226 152 2109 1697 Fragments arranged by size: 2109 1697 812 369 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2600 2690 1870 Size: 73 657 90 4545 Fragments arranged by size: 4545 657 90 73 BamHI G'GATC_C Cuts at: 2695 3062 2695 Size: 367 4998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2686 4141 618 Size: 325 217 627 899 1455 1842 Fragments arranged by size: 1842 1455 899 627 325 217 BanII G_rGCy'C Cuts at: 1391 2607 2684 1391 Size: 1216 77 4072 Fragments arranged by size: 4072 1216 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3556 3590 1903 Size: 34 50 34 1535 34 3678 Fragments arranged by size: 3678 1535 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2367 3257 4467 2367 Size: 890 1210 3265 Fragments arranged by size: 3265 1210 890 BclI T'GATC_A Cuts at: 1548 1548 Size: 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3976 136 Size: 107 122 71 631 605 2304 1525 Fragments arranged by size: 2304 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5360 5360 Size: 5365 BlpI GC'TnA_GC Cuts at: 2782 2782 Size: 5365 Bme1580I G_kGCm'C Cuts at: 1790 2607 3426 4582 1790 Size: 817 819 1156 2573 Fragments arranged by size: 2573 1156 819 817 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3096 4054 442 Size: 416 53 83 399 24 1679 958 1753 Fragments arranged by size: 1753 1679 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 4025 1239 Size: 1046 1740 2579 Fragments arranged by size: 2579 1740 1046 Bpu10I CC'TnA_GC Cuts at: 2009 2117 2009 Size: 108 5257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2131 3490 4355 4653 2131 Size: 1359 865 298 2843 Fragments arranged by size: 2843 1359 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4028 882 Size: 617 2529 2219 Fragments arranged by size: 2529 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3156 493 Size: 711 1952 2702 Fragments arranged by size: 2702 1952 711 BsaBI GATnn'nnATC Cuts at: 5359 5359 Size: 5365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3172 3554 4189 275 Size: 53 83 186 936 1639 382 635 1451 Fragments arranged by size: 1639 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3798 4196 4470 4617 5028 3798 Size: 398 274 147 411 4135 Fragments arranged by size: 4135 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5140 Fragments arranged by size: 5140 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2352 2557 2566 1186 Size: 1166 205 9 3985 Fragments arranged by size: 3985 1166 205 9 BseYI C'CCAG_C Cuts at: 2223 4568 5064 2223 Size: 2345 496 2524 Fragments arranged by size: 2524 2345 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2549 1163 Size: 573 813 3979 Fragments arranged by size: 3979 813 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2815 3576 4604 1540 Size: 275 186 814 761 1028 2301 Fragments arranged by size: 2301 1028 814 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3426 3511 4582 3426 Size: 85 1071 4209 Fragments arranged by size: 4209 1071 85 BsiWI C'GTAC_G Cuts at: 2097 2097 Size: 5365 BsmI GAATG_Cn' Cuts at: 2876 2969 2876 Size: 93 5272 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 2684 3426 3511 4582 1391 Size: 399 817 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 817 742 399 85 77 BspEI T'CCGG_A Cuts at: 5028 5028 Size: 5365 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2720 844 Size: 1876 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3252 1394 Size: 429 1429 3507 Fragments arranged by size: 3507 1429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2284 2395 3860 4034 65 Size: 1183 1036 111 1465 174 1396 Fragments arranged by size: 1465 1396 1183 1036 174 111 BsrFI r'CCGG_y Cuts at: 1700 1810 4009 4196 1700 Size: 110 2199 187 2869 Fragments arranged by size: 2869 2199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5365 BssSI C'ACGA_G Cuts at: 3425 4437 3425 Size: 1012 4353 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5365 BstBI TT'CG_AA Cuts at: 4169 4169 Size: 5365 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstYI r'GATC_y Cuts at: 1972 2695 3062 3447 3464 4916 5002 5014 5360 Size: 723 367 385 17 1452 86 12 346 Cuts at: 5360 1972 Size: 1977 Fragments arranged by size: 1977 1452 723 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4813 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4626 Fragments arranged by size: 4626 683 56 BtsI GCAGTG_nn' Cuts at: 2881 3675 3695 2881 Size: 794 20 4551 Fragments arranged by size: 4551 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2108 4372 809 Size: 507 792 2264 1802 Fragments arranged by size: 2264 1802 792 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2812 3701 5330 Size: 54 1425 50 275 18 982 889 1629 Cuts at: 5330 8 Size: 43 Fragments arranged by size: 1629 1425 982 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2812 1537 Size: 275 1000 4090 Fragments arranged by size: 4090 1000 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 2378 3295 1353 Size: 794 231 917 3423 Fragments arranged by size: 3423 917 794 231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3952 4326 4472 387 Size: 1064 138 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2285 3428 4025 4812 5080 1239 Size: 393 584 69 1143 597 787 268 1524 Fragments arranged by size: 1524 1143 787 597 584 393 268 69 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 4512 1678 Size: 2834 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4006 4038 4080 4112 1373 Size: 32 135 32 2434 32 42 32 2626 Fragments arranged by size: 2626 2434 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2113 2317 2709 2955 669 Size: 988 281 175 204 392 246 3079 Fragments arranged by size: 3079 988 392 281 246 204 175 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2317 2955 2317 Size: 638 4727 KpnI G_GTAC'C Cuts at: 2690 2690 Size: 5365 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4479 4663 1305 Size: 3174 184 2007 Fragments arranged by size: 3174 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3543 Fragments arranged by size: 3543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3323 3682 3841 518 Size: 648 1416 741 359 159 2042 Fragments arranged by size: 2042 1416 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2781 3458 4606 4851 1606 Size: 1175 677 1148 245 2120 Fragments arranged by size: 2120 1175 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4813 NdeI CA'TA_TG Cuts at: 387 387 Size: 5365 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5365 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2723 4268 1132 Size: 97 1494 1545 2229 Fragments arranged by size: 2229 1545 1494 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5365 PciI A'CATG_T Cuts at: 4264 4264 Size: 5365 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5365 PfoI T'CCnGG_A Cuts at: 2443 2443 Size: 5365 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5365 PsiI TTA'TAA Cuts at: 2935 2935 Size: 5365 PspOMI G'GGCC_C Cuts at: 2603 2603 Size: 5365 PstI C_TGCA'G Cuts at: 830 2717 830 Size: 1887 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 SalI G'TCGA_C Cuts at: 1936 2707 1936 Size: 771 4594 SapI GCTCTTCn'nnn_ Cuts at: 2378 2378 Size: 5365 SbfI CC_TGCA'GG Cuts at: 2717 2717 Size: 5365 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2500 2713 3848 4529 4720 826 Size: 219 1455 213 1135 681 191 1471 Fragments arranged by size: 1471 1455 1135 681 219 213 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2602 2692 1872 Size: 730 90 4545 Fragments arranged by size: 4545 730 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 2146 3469 4370 4632 820 Size: 197 926 203 1323 901 262 1553 Fragments arranged by size: 1553 1323 926 901 262 203 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2723 1132 Size: 1591 3774 SspI AAT'ATT Cuts at: 5 52 3289 5 Size: 47 3237 2081 Fragments arranged by size: 3237 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5365 StyI C'CwwG_G Cuts at: 513 1065 2804 513 Size: 552 1739 3074 Fragments arranged by size: 3074 1739 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2455 2527 3392 3409 3562 3747 4192 Size: 1255 875 72 865 17 153 185 445 Cuts at: 4192 325 Size: 1498 Fragments arranged by size: 1498 1255 875 865 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 5323 96 Size: 274 80 33 51 1443 1634 1712 138 Fragments arranged by size: 1712 1634 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5365 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2600 2690 1870 Size: 730 90 4545 Fragments arranged by size: 4545 730 90 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3173 276 Size: 53 83 186 936 1639 2468 Fragments arranged by size: 2468 1639 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI