################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AlwNI AocI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbvCI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HinfI HpaI KpnI MaeIII MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp45I Tsp509I Tth111I ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 491 ACACGT A^C[GA][CT]GT AlwI 1640 GGATC GGATC(4/5) Aor51HI 950 AGCGCT AGC^GCT ApaI 510 GGGCCC GGGCC^C Asp700I 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1406 TGCGCA TGC^GCA BbrPI 907 CACGTG CAC^GTG BciVI 1346 GTATCC GTATCC(6/5) BclI 1592 TGATCA T^GATCA BfaI 925 CTAG C^TAG BglII 473 AGATCT A^GATCT BlpI 1218 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1218 GCTGAGC GC^T[ACGT]AGC BsaAI 907 CACGTG [CT]AC^GT[GA] BssHII 375 GCGCGC G^CGCGC BssSI 1111 CACGAG CACGAG(-5/-1) CelII 1218 GCTGAGC GC^T[ACGT]AGC Eco47III 950 AGCGCT AGC^GCT EcoNI 921 CCTTCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1193 CCATGG C^C[AT][AT]GG FbaI 1592 TGATCA T^GATCA FspI 1406 TGCGCA TGC^GCA KspI 80 CCGCGG CCGC^GG MaeI 925 CTAG C^TAG MseI 734 TTAA T^TAA NcoI 1193 CCATGG C^CATGG NotI 16 GCGGCCGC GC^GGCCGC NspI 833 ACATGC [GA]CATG^[CT] PaeR7I 241 CTCGAG C^TCGAG PflMI 1052 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 907 CACGTG CAC^GTG PmlI 907 CACGTG CAC^GTG SacII 80 CCGCGG CCGC^GG ScaI 1136 AGTACT AGT^ACT SexAI 824 ACCTGGT A^CC[AT]GGT SmaI 582 CCCGGG CCC^GGG StuI 1478 AGGCCT AGG^CCT StyI 1193 CCATGG C^C[AT][AT]GG Tru9I 734 TTAA T^TAA Van91I 1052 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 924 TCTAGA T^CTAGA XhoI 241 CTCGAG C^TCGAG XmaCI 582 CCCGGG C^CCGGG XmaI 582 CCCGGG C^CCGGG XmnI 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1204 GTAGAC GT^[AC][GT]AC 1291 GTCTAC BalI 1043 TGGCCA TGG^CCA 1190 TGGCCA BanII 510 GGGCCC G[GA]GC[CT]^C 1569 GAGCCC BbsI 1417 GTCTTC GAAGAC(2/6) 1585 GTCTTC BcgI 170 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 329 GCACCGTGCTCG BpmI 1245 CTCCAG CTGGAG(16/14) 1390 CTGGAG BpuAI 1417 GTCTTC GAAGAC(2/6) 1585 GTCTTC BseRI 739 GAGGAG GAGGAG(10/8) 742 GAGGAG BsgI 1005 CTGCAC GTGCAG(16/14) 274 GTGCAG BsiEI 17 CGGCCG CG[GA][CT]^CG 1091 CGGCCG BsmAI 1711 GAGAC GTCTC(1/5) 1071 GTCTC BstXI 203 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1448 CCATCGTCCTGG BstYI 473 AGATCT [GA]^GATC[CT] 1640 GGATCT DrdI 1576 GACTTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1656 GACCCTGCAGTC EagI 17 CGGCCG C^GGCCG 1091 CGGCCG EclXI 17 CGGCCG C^GGCCG 1091 CGGCCG Eco52I 17 CGGCCG C^GGCCG 1091 CGGCCG MflI 473 AGATCT [GA]^GATC[CT] 1640 GGATCT MluNI 1043 TGGCCA TGG^CCA 1190 TGGCCA MscI 1043 TGGCCA TGG^CCA 1190 TGGCCA MslI 120 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1353 CACCATCATG PpuMI 5 GGGACCC [GA]G^G[AT]CC[CT] 1211 GGGACCT PstI 1513 CTGCAG CTGCA^G 1660 CTGCAG SfcI 1513 CTGCAG C^T[GA][CT]AG 1660 CTGCAG SfiI 1116 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1149 GGCCGCGTGGGCC SmlI 241 CTCGAG C^T[CT][GA]AG 640 CTCAAG XhoII 473 AGATCT [GA]^GATC[CT] 1640 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I ApaLI AspHI AvaI AvaII BanI BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI KasI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI TseI TspRI TthHB8I XcmI