(Circular) (Six-base) MAPSORT of: pFN21AE9517.seq Check: 2566 from: 1 to: 6427 pFN21AE9517 6427 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3210 3297 3770 1937 Size: 1273 87 473 4594 Fragments arranged by size: 4594 1273 473 87 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2833 3409 4490 5874 6142 1632 Size: 1201 576 1081 1384 268 1917 Fragments arranged by size: 1917 1384 1201 1081 576 268 AfeI AGC'GCT Cuts at: 2957 2957 Size: 6427 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 2496 5326 6303 1203 Size: 1293 2830 977 1327 Fragments arranged by size: 2830 1327 1293 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2147 3680 1184 Size: 963 1533 3931 Fragments arranged by size: 3931 1533 963 AlwNI CAG_nnn'CTG Cuts at: 4170 5742 4170 Size: 1572 4855 ApaI G_GGCC'C Cuts at: 2519 2519 Size: 6427 ApaLI G'TGCA_C Cuts at: 2173 2252 2332 2462 4484 5640 2173 Size: 79 80 130 2022 1156 2960 Fragments arranged by size: 2960 2022 1156 130 80 79 ApoI r'AATT_y Cuts at: 1493 3736 3962 4114 6223 1493 Size: 2243 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2246 2432 2587 3752 1870 Size: 73 303 186 155 1165 4545 Fragments arranged by size: 4545 1165 303 186 155 73 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2650 2696 2993 3283 Size: 325 217 627 303 560 46 297 290 Cuts at: 3283 3377 3675 3748 5203 618 Size: 94 298 73 1455 1842 Fragments arranged by size: 1842 1455 627 560 325 303 298 297 290 217 94 73 46 BanII G_rGCy'C Cuts at: 1391 2519 3578 3746 1391 Size: 1128 1059 168 4072 Fragments arranged by size: 4072 1128 1059 168 BbeI G_GCGC'C Cuts at: 2094 2700 3287 3679 2094 Size: 606 587 392 4842 Fragments arranged by size: 4842 606 587 392 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3415 3583 928 Size: 971 1516 168 3772 Fragments arranged by size: 3772 1516 971 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2164 2198 2323 2357 4618 Size: 34 50 34 143 34 125 34 2261 Cuts at: 4618 4652 1903 Size: 34 3678 Fragments arranged by size: 3678 2261 143 125 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3362 4319 5529 3362 Size: 957 1210 4260 Fragments arranged by size: 4260 1210 957 BclI T'GATC_A Cuts at: 1548 3597 1548 Size: 2049 4378 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2437 2523 3128 Size: 107 122 71 631 605 765 86 605 Cuts at: 3128 3161 3555 5038 136 Size: 33 394 1483 1525 Fragments arranged by size: 1525 1483 765 631 605 605 394 122 107 86 71 33 BglII A'GATC_T Cuts at: 2478 6422 2478 Size: 3944 2483 BlpI GC'TnA_GC Cuts at: 3224 3844 3224 Size: 620 5807 Bme1580I G_kGCm'C Cuts at: 1790 2056 2177 2256 2336 2466 2519 2655 2996 Size: 266 121 79 80 130 53 136 341 Cuts at: 2996 3171 3447 3546 4488 5644 1790 Size: 175 276 99 942 1156 2573 Fragments arranged by size: 2573 1156 942 341 276 266 175 136 130 121 99 80 79 53 BmgBI CAC'GTC Cuts at: 1318 2499 3315 3525 1318 Size: 1181 816 210 4220 Fragments arranged by size: 4220 1181 816 210 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3508 4158 5116 Size: 416 53 83 399 24 2091 650 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 2091 1753 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2977 3151 2977 Size: 174 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3235 3416 5087 1239 Size: 1996 181 1671 2579 Fragments arranged by size: 2579 1996 1671 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 4552 5417 5715 2630 Size: 1922 865 298 3342 Fragments arranged by size: 3342 1922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5090 882 Size: 617 3591 2219 Fragments arranged by size: 3591 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2914 4218 493 Size: 711 1710 1304 2702 Fragments arranged by size: 2702 1710 1304 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2611 2697 3284 Size: 53 83 186 936 558 520 86 587 Cuts at: 3284 3420 3676 4234 4616 5251 275 Size: 136 256 558 382 635 1451 Fragments arranged by size: 1451 936 635 587 558 558 520 382 256 186 136 86 83 53 BsaWI w'CCGG_w Cuts at: 4860 5258 5532 5679 6090 4860 Size: 398 274 147 411 5197 Fragments arranged by size: 5197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6202 Fragments arranged by size: 6202 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2759 2762 1186 Size: 1573 3 4851 Fragments arranged by size: 4851 1573 3 BseYI C'CCAG_C Cuts at: 2071 2215 5630 6126 2071 Size: 144 3415 496 2372 Fragments arranged by size: 3415 2372 496 144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2995 1163 Size: 573 564 695 4595 Fragments arranged by size: 4595 695 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2025 3099 3877 4638 5666 1540 Size: 275 186 24 1074 778 761 1028 2301 Fragments arranged by size: 2301 1074 1028 778 761 275 186 24 BsiHKAI G_wGCw'C Cuts at: 2177 2256 2336 2343 2466 3033 3435 3498 4488 Size: 79 80 7 123 567 402 63 990 Cuts at: 4488 4573 5644 2177 Size: 85 1071 2960 Fragments arranged by size: 2960 1071 990 567 402 123 85 80 79 63 7 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2177 2256 2336 2343 2466 2519 Size: 399 266 121 79 80 7 123 53 Cuts at: 2519 2655 2996 3033 3171 3435 3447 3498 3546 Size: 136 341 37 138 264 12 51 48 Cuts at: 3546 3578 3746 4488 4573 5644 1391 Size: 32 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 266 264 168 138 136 123 121 85 80 79 53 51 48 37 32 12 7 BspEI T'CCGG_A Cuts at: 6090 6090 Size: 6427 BspHI T'CATG_A Cuts at: 2406 3363 3594 4316 6046 2406 Size: 957 231 722 1730 2787 Fragments arranged by size: 2787 1730 957 722 231 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 2565 2574 3228 3782 844 Size: 1376 345 9 654 554 3489 Fragments arranged by size: 3489 1376 654 554 345 9 BsrBI CCG'CTC Cuts at: 1394 1823 2027 2437 2863 3228 4314 1394 Size: 429 204 410 426 365 1086 3507 Fragments arranged by size: 3507 1086 429 426 410 365 204 BsrDI GCAATG_nn' Cuts at: 65 1248 4922 5096 65 Size: 1183 3674 174 1396 Fragments arranged by size: 3674 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2918 3098 3329 5071 5258 1700 Size: 110 1108 180 231 1742 187 2869 Fragments arranged by size: 2869 1742 1108 231 187 180 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 2380 1925 Size: 455 5972 BssSI C'ACGA_G Cuts at: 3116 4487 5499 3116 Size: 1371 1012 4044 Fragments arranged by size: 4044 1371 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6427 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6427 BstXI CCAn_nnnn'nTGG Cuts at: 2215 3460 2215 Size: 1245 5182 BstYI r'GATC_y Cuts at: 1972 2478 3645 3757 4124 4509 4526 5978 6064 Size: 506 1167 112 367 385 17 1452 86 Cuts at: 6064 6076 6422 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1167 506 385 367 346 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2085 2110 2140 2904 3198 513 Size: 552 1020 25 30 764 294 3742 Fragments arranged by size: 3742 1020 764 552 294 30 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2167 487 Size: 683 56 941 4747 Fragments arranged by size: 4747 941 683 56 BtsI GCAGTG_nn' Cuts at: 2288 2532 3943 4737 4757 2288 Size: 244 1411 794 20 3958 Fragments arranged by size: 3958 1411 794 244 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3587 3667 5434 809 Size: 507 2271 80 1767 1802 Fragments arranged by size: 2271 1802 1767 507 80 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2901 2916 Size: 54 1425 50 275 18 192 879 15 Cuts at: 2916 3048 3096 3153 3195 3327 3556 3874 4763 Size: 132 48 57 42 132 229 318 889 Cuts at: 4763 6392 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 879 318 275 229 192 132 132 57 54 50 48 43 42 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2022 3096 3874 1537 Size: 275 210 1074 778 4090 Fragments arranged by size: 4090 1074 778 275 210 EarI CTCTTCn'nnn_ Cuts at: 1353 3026 3308 3350 4357 1353 Size: 1673 282 42 1007 3423 Fragments arranged by size: 3423 1673 1007 282 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2833 3235 3409 3416 4490 5087 5874 Size: 393 1201 402 174 7 1074 597 787 Cuts at: 5874 6142 1239 Size: 268 1524 Fragments arranged by size: 1524 1201 1074 787 597 402 393 268 174 7 EcoNI CCTnn'n_nnAGG Cuts at: 2930 2930 Size: 6427 EcoO109I rG'GnC_Cy Cuts at: 2011 2515 2592 3217 2011 Size: 504 77 625 5221 Fragments arranged by size: 5221 625 504 77 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2437 2437 Size: 6427 FspI TGC'GCA Cuts at: 3413 4933 3413 Size: 1520 4907 FspAI rTGC'GCAy Cuts at: 3413 3413 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 2069 2094 2405 2700 2758 2848 2959 3287 Size: 391 25 311 295 58 90 111 328 Cuts at: 3287 3679 5574 1678 Size: 392 1895 2531 Fragments arranged by size: 2531 1895 392 391 328 311 295 111 90 58 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5068 5100 5142 5174 1373 Size: 32 135 32 3496 32 42 32 2626 Fragments arranged by size: 3496 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6427 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KasI G'GCGC_C Cuts at: 2090 2696 3283 3675 2090 Size: 606 587 392 4842 Fragments arranged by size: 4842 606 587 392 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2481 5541 5725 1305 Size: 1176 3060 184 2007 Fragments arranged by size: 3060 2007 1176 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3050 3197 10 Size: 54 1425 343 1218 147 3240 Fragments arranged by size: 3240 1425 1218 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 3362 4385 4744 4903 518 Size: 648 963 1233 1023 359 159 2042 Fragments arranged by size: 2042 1233 1023 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 2170 2269 3843 4520 5668 5913 1606 Size: 481 83 99 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 481 245 99 83 NaeI GCC'GGC Cuts at: 2920 3100 3331 2920 Size: 180 231 6016 Fragments arranged by size: 6016 231 180 NarI GG'CG_CC Cuts at: 2091 2697 3284 3676 2091 Size: 606 587 392 4842 Fragments arranged by size: 4842 606 587 392 NcoI C'CATG_G Cuts at: 513 1065 3198 513 Size: 552 2133 3742 Fragments arranged by size: 3742 2133 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NgoMIV G'CCGG_C Cuts at: 2918 3098 3329 2918 Size: 180 231 6016 Fragments arranged by size: 6016 231 180 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 2022 3874 2022 Size: 1852 4575 NspI r_CATG'y Cuts at: 1132 1229 2842 3785 5330 1132 Size: 97 1613 943 1545 2229 Fragments arranged by size: 2229 1613 1545 943 97 PasI CC'CwG_GG Cuts at: 1348 2041 1348 Size: 693 5734 PciI A'CATG_T Cuts at: 5326 5326 Size: 6427 PflMI CCAn_nnn'nTGG Cuts at: 1108 3063 1108 Size: 1955 4472 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PmlI CAC'GTG Cuts at: 2914 2914 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 3217 2011 Size: 1206 5221 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspOMI G'GGCC_C Cuts at: 2515 2515 Size: 6427 PspXI vC'TCGA_Gb Cuts at: 2246 2246 Size: 6427 PstI C_TGCA'G Cuts at: 830 3522 3669 3779 830 Size: 2692 147 110 3478 Fragments arranged by size: 3478 2692 147 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 SacII CC_GC'GG Cuts at: 2088 2088 Size: 6427 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SanDI GG'GwC_CC Cuts at: 2011 2011 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 3143 4675 6387 3143 Size: 1532 1712 3183 Fragments arranged by size: 3183 1712 1532 SexAI A'CCwGG_T Cuts at: 2829 2829 Size: 6427 SfcI C'TryA_G Cuts at: 826 1045 3518 3665 3775 4910 5591 5782 826 Size: 219 2473 147 110 1135 681 191 1471 Fragments arranged by size: 2473 1471 1135 681 219 191 147 110 SfiI GGCCn_nnn'nGGCC Cuts at: 3128 3161 3128 Size: 33 6394 SfoI GGC'GCC Cuts at: 2092 2698 3285 3677 2092 Size: 606 587 392 4842 Fragments arranged by size: 4842 606 587 392 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 2589 3754 1872 Size: 717 1165 4545 Fragments arranged by size: 4545 1165 717 SmlI C'TyrA_G Cuts at: 820 1017 1943 2246 2645 4531 5432 5694 820 Size: 197 926 303 399 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 399 303 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 3485 1517 Size: 1968 4459 StyI C'CwwG_G Cuts at: 513 1065 3198 3866 513 Size: 552 2133 668 3074 Fragments arranged by size: 3074 2133 668 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2669 2761 3401 4454 4471 4624 4809 Size: 1255 1089 92 640 1053 17 153 185 Cuts at: 4809 5254 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1089 1053 640 445 185 153 92 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2733 3141 4673 Size: 274 80 33 51 1443 756 408 1532 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 1712 1532 1443 756 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 2929 3763 2929 Size: 834 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 3190 3457 3022 Size: 168 267 5992 Fragments arranged by size: 5992 267 168 XhoI C'TCGA_G Cuts at: 1943 2246 1943 Size: 303 6124 XmaI C'CCGG_G Cuts at: 1870 2587 3752 1870 Size: 717 1165 4545 Fragments arranged by size: 4545 1165 717 XmnI GAAnn'nnTTC Cuts at: 2414 4556 2414 Size: 2142 4285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PfoI PsrI PvuII RsrII SacI SapI SrfI SwaI