(Linear) (Six-base) MAPSORT of: KIEE9517.seq Check: 8422 from: 1 to: 1722 KIEE9517.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AccI GT'mk_AC Cuts at: 0 1205 1292 1722 Size: 1205 87 430 Fragments arranged by size: 1205 430 87 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 828 1404 1722 Size: 828 576 318 Fragments arranged by size: 828 576 318 AfeI AGC'GCT Cuts at: 0 952 1722 Size: 952 770 AflIII A'CryG_T Cuts at: 0 491 1722 Size: 491 1231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 142 1675 1722 Size: 142 1533 47 Fragments arranged by size: 1533 142 47 ApaI G_GGCC'C Cuts at: 0 514 1722 Size: 514 1208 ApaLI G'TGCA_C Cuts at: 0 168 247 327 457 1722 Size: 168 79 80 130 1265 Fragments arranged by size: 1265 168 130 80 79 AvaI C'yCGr_G Cuts at: 0 241 427 582 1722 Size: 241 186 155 1140 Fragments arranged by size: 1140 241 186 155 BanI G'GyrC_C Cuts at: 0 85 645 691 988 1278 1372 1670 1722 Size: 85 560 46 297 290 94 298 52 Fragments arranged by size: 560 298 297 290 94 85 52 46 BanII G_rGCy'C Cuts at: 0 514 1573 1722 Size: 514 1059 149 Fragments arranged by size: 1059 514 149 BbeI G_GCGC'C Cuts at: 0 89 695 1282 1674 1722 Size: 89 606 587 392 48 Fragments arranged by size: 606 587 392 89 48 BbsI GAAGACnn'nnnn_ Cuts at: 0 1410 1578 1722 Size: 1410 168 144 Fragments arranged by size: 1410 168 144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 159 193 318 352 1722 Size: 159 34 125 34 1370 Fragments arranged by size: 1370 159 125 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1357 1722 Size: 1357 365 BclI T'GATC_A Cuts at: 0 1592 1722 Size: 1592 130 BglI GCCn_nnn'nGGC Cuts at: 0 432 518 1123 1156 1550 1722 Size: 432 86 605 33 394 172 Fragments arranged by size: 605 432 394 172 86 33 BglII A'GATC_T Cuts at: 0 473 1722 Size: 473 1249 BlpI GC'TnA_GC Cuts at: 0 1219 1722 Size: 1219 503 Bme1580I G_kGCm'C Cuts at: 0 51 172 251 331 461 514 650 991 Size: 51 121 79 80 130 53 136 341 Cuts at: 991 1166 1442 1541 1722 Size: 175 276 99 181 Fragments arranged by size: 341 276 181 175 136 130 121 99 80 79 53 51 BmgBI CAC'GTC Cuts at: 0 494 1310 1520 1722 Size: 494 816 210 202 Fragments arranged by size: 816 494 210 202 BmrI ACTGGGnnnn_n' Cuts at: 0 1503 1722 Size: 1503 219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 972 1146 1722 Size: 972 174 576 Fragments arranged by size: 972 576 174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1230 1411 1722 Size: 1230 181 311 Fragments arranged by size: 1230 311 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 1722 Size: 625 1097 BsaAI yAC'GTr Cuts at: 0 909 1722 Size: 909 813 BsaHI Gr'CG_yC Cuts at: 0 86 606 692 1279 1415 1671 1722 Size: 86 520 86 587 136 256 51 Fragments arranged by size: 587 520 256 136 86 86 51 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 754 757 1722 Size: 754 3 965 Fragments arranged by size: 965 754 3 BseYI C'CCAG_C Cuts at: 0 66 210 1722 Size: 66 144 1512 Fragments arranged by size: 1512 144 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 990 1722 Size: 295 695 732 Fragments arranged by size: 732 695 295 BsiEI CG_ry'CG Cuts at: 0 20 1094 1722 Size: 20 1074 628 Fragments arranged by size: 1074 628 20 BsiHKAI G_wGCw'C Cuts at: 0 172 251 331 338 461 1028 1430 1493 Size: 172 79 80 7 123 567 402 63 Cuts at: 1493 1722 Size: 229 Fragments arranged by size: 567 402 229 172 123 80 79 63 7 Bsp1286I G_dGCh'C Cuts at: 0 51 172 251 331 338 461 514 650 Size: 51 121 79 80 7 123 53 136 Cuts at: 650 991 1028 1166 1430 1442 1493 1541 1573 Size: 341 37 138 264 12 51 48 32 Cuts at: 1573 1722 Size: 149 Fragments arranged by size: 341 264 149 138 136 123 121 80 79 53 51 51 48 37 32 12 7 BspHI T'CATG_A Cuts at: 0 401 1358 1589 1722 Size: 401 957 231 133 Fragments arranged by size: 957 401 231 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 215 560 569 1223 1722 Size: 215 345 9 654 499 Fragments arranged by size: 654 499 345 215 9 BsrBI CCG'CTC Cuts at: 0 22 432 858 1223 1722 Size: 22 410 426 365 499 Fragments arranged by size: 499 426 410 365 22 BsrFI r'CCGG_y Cuts at: 0 913 1093 1324 1722 Size: 913 180 231 398 Fragments arranged by size: 913 398 231 180 BssHII G'CGCG_C Cuts at: 0 375 1722 Size: 375 1347 BssSI C'ACGA_G Cuts at: 0 1111 1722 Size: 1111 611 BstXI CCAn_nnnn'nTGG Cuts at: 0 210 1455 1722 Size: 210 1245 267 Fragments arranged by size: 1245 267 210 BstYI r'GATC_y Cuts at: 0 473 1640 1722 Size: 473 1167 82 Fragments arranged by size: 1167 473 82 BtgI C'CryG_G Cuts at: 0 80 105 135 899 1193 1722 Size: 80 25 30 764 294 529 Fragments arranged by size: 764 529 294 80 30 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 162 1722 Size: 162 1560 BtsI GCAGTG_nn' Cuts at: 0 283 527 1722 Size: 283 244 1195 Fragments arranged by size: 1195 283 244 DrdI GACnn_nn'nnGTC Cuts at: 0 1582 1662 1722 Size: 1582 80 60 Fragments arranged by size: 1582 80 60 EaeI y'GGCC_r Cuts at: 0 17 896 911 1043 1091 1148 1190 1322 Size: 17 879 15 132 48 57 42 132 Cuts at: 1322 1551 1722 Size: 229 171 Fragments arranged by size: 879 229 171 132 132 57 48 42 17 15 EagI C'GGCC_G Cuts at: 0 17 1091 1722 Size: 17 1074 631 Fragments arranged by size: 1074 631 17 EarI CTCTTCn'nnn_ Cuts at: 0 1021 1303 1345 1722 Size: 1021 282 42 377 Fragments arranged by size: 1021 377 282 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 828 1230 1404 1411 1722 Size: 828 402 174 7 311 Fragments arranged by size: 828 402 311 174 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 925 1722 Size: 925 797 EcoO109I rG'GnC_Cy Cuts at: 0 6 510 587 1212 1722 Size: 6 504 77 625 510 Fragments arranged by size: 625 510 504 77 6 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 432 1722 Size: 432 1290 FspI TGC'GCA Cuts at: 0 1408 1722 Size: 1408 314 FspAI rTGC'GCAy Cuts at: 0 1408 1722 Size: 1408 314 HaeII r_GCGC'y Cuts at: 0 64 89 400 695 753 843 954 1282 Size: 64 25 311 295 58 90 111 328 Cuts at: 1282 1674 1722 Size: 392 48 Fragments arranged by size: 392 328 311 295 111 90 64 58 48 25 KasI G'GCGC_C Cuts at: 0 85 691 1278 1670 1722 Size: 85 606 587 392 52 Fragments arranged by size: 606 587 392 85 52 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 476 1722 Size: 476 1246 MscI TGG'CCA Cuts at: 0 1045 1192 1722 Size: 1045 147 530 Fragments arranged by size: 1045 530 147 MslI CAynn'nnrTG Cuts at: 0 124 1357 1722 Size: 124 1233 365 Fragments arranged by size: 1233 365 124 MspA1I CmG'CkG Cuts at: 0 82 165 264 1722 Size: 82 83 99 1458 Fragments arranged by size: 1458 99 83 82 NaeI GCC'GGC Cuts at: 0 915 1095 1326 1722 Size: 915 180 231 396 Fragments arranged by size: 915 396 231 180 NarI GG'CG_CC Cuts at: 0 86 692 1279 1671 1722 Size: 86 606 587 392 51 Fragments arranged by size: 606 587 392 86 51 NcoI C'CATG_G Cuts at: 0 1193 1722 Size: 1193 529 NgoMIV G'CCGG_C Cuts at: 0 913 1093 1324 1722 Size: 913 180 231 398 Fragments arranged by size: 913 398 231 180 NotI GC'GGCC_GC Cuts at: 0 17 1722 Size: 17 1705 NspI r_CATG'y Cuts at: 0 837 1722 Size: 837 885 PasI CC'CwG_GG Cuts at: 0 36 1722 Size: 36 1686 PflMI CCAn_nnn'nTGG Cuts at: 0 1058 1722 Size: 1058 664 PmlI CAC'GTG Cuts at: 0 909 1722 Size: 909 813 PpuMI rG'GwC_Cy Cuts at: 0 6 1212 1722 Size: 6 1206 510 Fragments arranged by size: 1206 510 6 PspOMI G'GGCC_C Cuts at: 0 510 1722 Size: 510 1212 PspXI vC'TCGA_Gb Cuts at: 0 241 1722 Size: 241 1481 PstI C_TGCA'G Cuts at: 0 1517 1664 1722 Size: 1517 147 58 Fragments arranged by size: 1517 147 58 SacII CC_GC'GG Cuts at: 0 83 1722 Size: 83 1639 SanDI GG'GwC_CC Cuts at: 0 6 1722 Size: 6 1716 ScaI AGT'ACT Cuts at: 0 1138 1722 Size: 1138 584 SexAI A'CCwGG_T Cuts at: 0 824 1722 Size: 824 898 SfcI C'TryA_G Cuts at: 0 1513 1660 1722 Size: 1513 147 62 Fragments arranged by size: 1513 147 62 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1123 1156 1722 Size: 1123 33 566 Fragments arranged by size: 1123 566 33 SfoI GGC'GCC Cuts at: 0 87 693 1280 1672 1722 Size: 87 606 587 392 50 Fragments arranged by size: 606 587 392 87 50 SmaI CCC'GGG Cuts at: 0 584 1722 Size: 584 1138 SmlI C'TyrA_G Cuts at: 0 241 640 1722 Size: 241 399 1082 Fragments arranged by size: 1082 399 241 StuI AGG'CCT Cuts at: 0 1480 1722 Size: 1480 242 StyI C'CwwG_G Cuts at: 0 1193 1722 Size: 1193 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 664 756 1396 1722 Size: 664 92 640 326 Fragments arranged by size: 664 640 326 92 TatI w'GTAC_w Cuts at: 0 728 1136 1722 Size: 728 408 586 Fragments arranged by size: 728 586 408 XbaI T'CTAG_A Cuts at: 0 924 1722 Size: 924 798 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1017 1185 1452 1722 Size: 1017 168 267 270 Fragments arranged by size: 1017 270 267 168 XhoI C'TCGA_G Cuts at: 0 241 1722 Size: 241 1481 XmaI C'CCGG_G Cuts at: 0 582 1722 Size: 582 1140 XmnI GAAnn'nnTTC Cuts at: 0 409 1722 Size: 409 1313 Enzymes that do cut: AccI AcuI AfeI AflIII AlfI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PmlI PpuMI PspOMI PspXI PstI SacII SanDI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BfrBI BmtI Bpu10I BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I ZraI