################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SspI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 682 CCGC CCGC(-3/-1) Alw44I 740 GTGCAC G^TGCAC AlwNI 1378 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 740 GTGCAC G^TGCAC AspHI 740 GTGCAC G[AT]GC[AT]^C BalI 1319 TGGCCA TGG^CCA BanI 1263 GGCACC G^G[CT][GA]CC BcgI 1236 CGAAGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1339 GTATCC GTATCC(6/5) BlpI 167 GCTCAGC GC^T[ACGT]AGC Bpu1102I 167 GCTCAGC GC^T[ACGT]AGC BsaI 753 GGTCTC GGTCTC(1/5) BseRI 244 CTCCTC GAGGAG(10/8) BsiHKAI 740 GTGCAC G[AT]GC[AT]^C Bsp1407I 824 TGTACA T^GTACA BsrGI 824 TGTACA T^GTACA BstXI 942 CCAGTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 167 GCTCAGC GC^T[ACGT]AGC DsaI 1071 CCATGG C^C[GA][CT]GG EaeI 1319 TGGCCA [CT]^GGCC[GA] HindIII 67 AAGCTT A^AGCTT HphI 1207 GGTGA GGTGA(8/7) MluNI 1319 TGGCCA TGG^CCA MscI 1319 TGGCCA TGG^CCA NcoI 1071 CCATGG C^CATGG PflMI 99 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 512 AGGACCT [GA]G^G[AT]CC[CT] SbfI 1275 CCTGCAGG CCTGCA^GG SphI 705 GCATGC GCATG^C Sse8387I 1275 CCTGCAGG CCTGCA^GG SspBI 824 TGTACA T^GTACA Tsp509I 1403 AATT ^AATT Van91I 99 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 465 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 601 GTAGAC GT^[AC][GT]AC 1090 GTCTAC AlwI 924 GATCC GGATC(4/5) 204 GGATC BbsI 220 GAAGAC GAAGAC(2/6) 1420 GAAGAC BpuAI 220 GAAGAC GAAGAC(2/6) 1420 GAAGAC BspMI 350 ACCTGC ACCTGC(4/8) 709 GCAGGT BstYI 204 GGATCT [GA]^GATC[CT] 923 AGATCC DraII 512 AGGACCT [GA]G^G[ACGT]CC[CT] 1106 AGGCCCT EcoO109I 512 AGGACCT [GA]G^G[ACGT]CC[CT] 1106 AGGCCCT EcoT14I 1071 CCATGG C^C[AT][AT]GG 1203 CCAAGG MflI 204 GGATCT [GA]^GATC[CT] 923 AGATCC MseI 391 TTAA T^TAA 659 TTAA MslI 910 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 970 CACAATAATG MspA1I 818 CAGCTG C[AC]G^C[GT]G 1365 CAGCTG PvuII 818 CAGCTG CAG^CTG 1365 CAGCTG SmlI 8 CTCAAG C^T[CT][GA]AG 115 CTCAAG SpeI 639 ACTAGT A^CTAGT 1530 ACTAGT StuI 849 AGGCCT AGG^CCT 1196 AGGCCT StyI 1071 CCATGG C^C[AT][AT]GG 1203 CCAAGG TaqI 1235 TCGA T^CGA 1464 TCGA TfiI 82 GATTC G^A[AT]TC 834 GAATC Tru9I 391 TTAA T^TAA 659 TTAA Tsp45I 589 GTGAC ^GT[GC]AC 979 GTGAC TthHB8I 1235 TCGA T^CGA 1464 TCGA XhoII 204 GGATCT [GA]^GATC[CT] 923 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AfaI AluI AvaII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI XcmI