(Circular) (Six-base) MAPSORT of: pFN21AE1960.seq Check: 7015 from: 1 to: 6271 pFN21AE1960 6271 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2607 3096 3614 1937 Size: 670 489 518 4594 Fragments arranged by size: 4594 670 518 489 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2915 3422 4334 5718 5986 1632 Size: 1283 507 912 1384 268 1917 Fragments arranged by size: 1917 1384 1283 912 507 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 5170 6147 1203 Size: 3967 977 1327 Fragments arranged by size: 3967 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 3388 4014 5586 3388 Size: 626 1572 4073 Fragments arranged by size: 4073 1572 626 ApaLI G'TGCA_C Cuts at: 2745 4328 5484 2745 Size: 1583 1156 3532 Fragments arranged by size: 3532 1583 1156 ApoI r'AATT_y Cuts at: 1493 3580 3806 3958 6067 1493 Size: 2087 226 152 2109 1697 Fragments arranged by size: 2109 2087 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3596 1870 Size: 73 1653 4545 Fragments arranged by size: 4545 1653 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2520 2553 2520 Size: 33 6238 BamHI G'GATC_C Cuts at: 3601 3968 3601 Size: 367 5904 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 3592 5047 618 Size: 325 217 627 1481 324 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 3590 1391 Size: 2199 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 3432 928 Size: 971 333 1200 3767 Fragments arranged by size: 3767 1200 971 333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3230 3264 4462 4496 1903 Size: 34 1293 34 1198 34 3678 Fragments arranged by size: 3678 1293 1198 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3355 4163 5373 3355 Size: 808 1210 4253 Fragments arranged by size: 4253 1210 808 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4882 136 Size: 107 122 71 631 605 3210 1525 Fragments arranged by size: 3210 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 2173 3688 2173 Size: 1515 4756 Bme1580I G_kGCm'C Cuts at: 1790 2749 3164 3552 4332 5488 1790 Size: 959 415 388 780 1156 2573 Fragments arranged by size: 2573 1156 959 780 415 388 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4002 4960 442 Size: 416 53 83 399 24 2585 958 1753 Fragments arranged by size: 2585 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2534 2746 4931 1239 Size: 941 354 212 2185 2579 Fragments arranged by size: 2579 2185 941 354 212 Bpu10I CC'TnA_GC Cuts at: 3261 3261 Size: 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1998 2105 4396 5261 5559 1998 Size: 107 2291 865 298 2710 Fragments arranged by size: 2710 2291 865 298 107 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2764 4934 882 Size: 617 1265 2170 2219 Fragments arranged by size: 2219 2170 1265 617 BsaAI yAC'GTr Cuts at: 493 1204 4062 493 Size: 711 2858 2702 Fragments arranged by size: 2858 2702 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4078 4460 5095 275 Size: 53 83 186 936 2545 382 635 1451 Fragments arranged by size: 2545 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3122 3152 1178 Size: 30 165 30 1719 30 4297 Fragments arranged by size: 4297 1719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 1186 Size: 1054 5217 BseYI C'CCAG_C Cuts at: 3507 5474 5970 3507 Size: 1967 496 3808 Fragments arranged by size: 3808 1967 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2749 4332 4417 5488 2749 Size: 1583 85 1071 3532 Fragments arranged by size: 3532 1583 1071 85 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2749 3164 3552 3590 4332 4417 5488 Size: 399 959 415 388 38 742 85 1071 Cuts at: 5488 1391 Size: 2174 Fragments arranged by size: 2174 1071 959 742 415 399 388 85 38 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 2705 3626 844 Size: 1520 341 921 3489 Fragments arranged by size: 3489 1520 921 341 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4915 5102 1700 Size: 110 3105 187 2869 Fragments arranged by size: 3105 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2829 96 Size: 2733 3538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6271 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstXI CCAn_nnnn'nTGG Cuts at: 2954 2954 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2209 2928 3601 3968 4353 4370 5822 5908 Size: 237 719 673 367 385 17 1452 86 Cuts at: 5908 5920 6266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 719 673 385 367 346 237 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3076 513 Size: 552 2011 3708 Fragments arranged by size: 3708 2011 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3164 3444 3787 4581 4601 3164 Size: 280 343 794 20 4834 Fragments arranged by size: 4834 794 343 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3324 3718 4607 Size: 54 1425 50 275 18 1494 394 889 Cuts at: 4607 6236 8 Size: 1629 43 Fragments arranged by size: 1629 1494 1425 889 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3106 3210 3466 4201 1353 Size: 1753 104 256 735 3423 Fragments arranged by size: 3423 1753 735 256 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2534 2746 2915 3422 4334 4931 Size: 393 548 354 212 169 507 912 597 Cuts at: 4931 5718 5986 1239 Size: 787 268 1524 Fragments arranged by size: 1524 912 787 597 548 507 393 354 268 212 169 EcoO109I rG'GnC_Cy Cuts at: 2518 3112 2518 Size: 594 5677 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2444 2689 2444 Size: 245 6026 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 5418 1678 Size: 3740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2920 2952 3122 3124 3154 Size: 32 135 32 1348 32 170 2 30 Cuts at: 3154 3156 3419 3451 4912 4944 4986 5018 1373 Size: 2 263 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 1348 263 170 135 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2021 2872 3454 3615 3861 669 Size: 988 281 83 851 582 161 246 3079 Fragments arranged by size: 3079 988 851 582 281 246 161 83 HindIII A'AGCT_T Cuts at: 748 2072 748 Size: 1324 4947 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5385 5569 1305 Size: 4080 184 2007 Fragments arranged by size: 4080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3326 10 Size: 54 1425 343 1494 2955 Fragments arranged by size: 2955 1494 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2919 2979 4229 4588 4747 518 Size: 648 1753 60 1250 359 159 2042 Fragments arranged by size: 2042 1753 1250 648 359 159 60 MspA1I CmG'CkG Cuts at: 1606 2825 3372 3687 4364 5512 5757 1606 Size: 1219 547 315 677 1148 245 2120 Fragments arranged by size: 2120 1219 1148 677 547 315 245 NcoI C'CATG_G Cuts at: 513 1065 3076 513 Size: 552 2011 3708 Fragments arranged by size: 3708 2011 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2511 2714 3359 3629 5174 1132 Size: 97 1282 203 645 270 1545 2229 Fragments arranged by size: 2229 1545 1282 645 270 203 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 1108 Size: 1002 5269 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 2518 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 2283 3015 3159 3285 3623 830 Size: 1453 732 144 126 338 3478 Fragments arranged by size: 3478 1453 732 338 144 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2825 3372 2825 Size: 547 5724 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3285 3623 3285 Size: 338 5933 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2279 2604 3011 3155 3281 3619 4754 Size: 219 1234 325 407 144 126 338 1135 Cuts at: 4754 5435 5626 826 Size: 681 191 1471 Fragments arranged by size: 1471 1234 1135 681 407 338 325 219 191 144 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2013 2120 4375 5276 5538 820 Size: 197 926 70 107 2255 901 262 1553 Fragments arranged by size: 2255 1553 926 901 262 197 107 70 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 2644 3535 152 Size: 2492 891 2888 Fragments arranged by size: 2888 2492 891 SphI G_CATG'C Cuts at: 1132 2714 3629 1132 Size: 1582 915 3774 Fragments arranged by size: 3774 1582 915 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 2856 3203 1517 Size: 1339 347 4585 Fragments arranged by size: 4585 1339 347 StyI C'CwwG_G Cuts at: 513 1065 3076 3208 3710 513 Size: 552 2011 132 502 3074 Fragments arranged by size: 3074 2011 552 502 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3286 4298 4315 4468 4653 5098 325 Size: 1255 1706 1012 17 153 185 445 1498 Fragments arranged by size: 1706 1498 1255 1012 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 4517 6229 Size: 274 80 33 51 1443 852 1688 1712 Cuts at: 6229 96 Size: 138 Fragments arranged by size: 1712 1688 1443 852 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 2470 3607 2470 Size: 1137 5134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2155 2305 3083 2155 Size: 150 778 5343 Fragments arranged by size: 5343 778 150 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 4400 4400 Size: 6271 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI