(Circular) (Six-base) MAPSORT of: pFN21AB8499.seq Check: 6773 from: 1 to: 7501 pFN21AB8499 7501 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5311 278 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2797 4609 4844 1937 Size: 860 1812 235 4594 Fragments arranged by size: 4594 1812 860 235 Acc65I G'GTAC_C Cuts at: 4822 4822 Size: 7501 AclI AA'CG_TT Cuts at: 1563 5628 6001 1563 Size: 4065 373 3063 Fragments arranged by size: 4065 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2712 3144 4034 5564 6948 7216 1632 Size: 1080 432 890 1530 1384 268 1917 Fragments arranged by size: 1917 1530 1384 1080 890 432 268 AflII C'TTAA_G Cuts at: 820 1017 2560 820 Size: 197 1543 5761 Fragments arranged by size: 5761 1543 197 AflIII A'CryG_T Cuts at: 1203 6400 7377 1203 Size: 5197 977 1327 Fragments arranged by size: 5197 1327 977 AgeI A'CCGG_T Cuts at: 6332 6332 Size: 7501 AhdI GACnn_n'nnGTC Cuts at: 6230 6230 Size: 7501 AleI CACnn'nnGTG Cuts at: 1166 2063 1166 Size: 897 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7501 AlwNI CAG_nnn'CTG Cuts at: 5244 6816 5244 Size: 1572 5929 ApaLI G'TGCA_C Cuts at: 5558 6714 5558 Size: 1156 6345 ApoI r'AATT_y Cuts at: 1493 2260 2929 3034 3276 3480 3520 3552 3985 Size: 767 669 105 242 204 40 32 433 Cuts at: 3985 4129 4560 4810 5036 5188 7297 1493 Size: 144 431 250 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 669 433 431 250 242 226 204 152 144 105 40 32 AseI AT'TA_AT Cuts at: 160 1032 6055 160 Size: 872 5023 1606 Fragments arranged by size: 5023 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 AvaI C'yCGr_G Cuts at: 1870 1943 4826 1870 Size: 73 2883 4545 Fragments arranged by size: 4545 2883 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2415 2448 3147 3180 3886 3919 2415 Size: 33 699 33 706 33 5997 Fragments arranged by size: 5997 706 699 33 33 33 BamHI G'GATC_C Cuts at: 4831 5198 4831 Size: 367 7134 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 4822 6277 618 Size: 325 217 627 324 2711 1455 1842 Fragments arranged by size: 2711 1842 1455 627 325 324 217 BanII G_rGCy'C Cuts at: 1391 4820 1391 Size: 3429 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3839 928 Size: 971 1940 4590 Fragments arranged by size: 4590 1940 971 BbvCI CC'TCA_GC Cuts at: 2316 2316 Size: 7501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5692 5726 1903 Size: 34 3755 34 3678 Fragments arranged by size: 3755 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2555 2889 5393 6603 2555 Size: 334 2504 1210 3453 Fragments arranged by size: 3453 2504 1210 334 BclI T'GATC_A Cuts at: 1548 1548 Size: 7501 BfrBI ATG'CAT Cuts at: 3187 3308 4208 3187 Size: 121 900 6480 Fragments arranged by size: 6480 900 121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6112 136 Size: 107 122 71 631 605 4440 1525 Fragments arranged by size: 4440 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2251 2524 7496 2251 Size: 273 4972 2256 Fragments arranged by size: 4972 2256 273 BlpI GC'TnA_GC Cuts at: 4918 4918 Size: 7501 Bme1580I G_kGCm'C Cuts at: 1790 5562 6718 1790 Size: 3772 1156 2573 Fragments arranged by size: 3772 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7501 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2356 3394 5232 Size: 416 53 83 399 24 939 1038 1838 Cuts at: 5232 6190 442 Size: 958 1753 Fragments arranged by size: 1838 1753 1038 958 939 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7501 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 6161 1239 Size: 917 4005 2579 Fragments arranged by size: 4005 2579 917 Bpu10I CC'TnA_GC Cuts at: 2316 2316 Size: 7501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4310 5626 6491 6789 4310 Size: 1316 865 298 5022 Fragments arranged by size: 5022 1316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6164 882 Size: 617 4665 2219 Fragments arranged by size: 4665 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5292 493 Size: 711 4088 2702 Fragments arranged by size: 4088 2702 711 BsaBI GATnn'nnATC Cuts at: 7495 7495 Size: 7501 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5308 5690 6325 275 Size: 53 83 186 936 3775 382 635 1451 Fragments arranged by size: 3775 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5934 6332 6606 6753 7164 5934 Size: 398 274 147 411 6271 Fragments arranged by size: 6271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4632 4662 1178 Size: 30 165 30 3229 30 4017 Fragments arranged by size: 4017 3229 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 1186 Size: 1333 6168 BseYI C'CCAG_C Cuts at: 6704 7200 6704 Size: 496 7005 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6928 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4951 5712 6740 1540 Size: 275 186 2950 761 1028 2301 Fragments arranged by size: 2950 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3742 5562 5647 6718 3742 Size: 1820 85 1071 4525 Fragments arranged by size: 4525 1820 1071 85 BsmI GAATG_Cn' Cuts at: 3310 5012 5105 3310 Size: 1702 93 5706 Fragments arranged by size: 5706 1702 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3742 4820 5562 5647 6718 1391 Size: 399 1952 1078 742 85 1071 2174 Fragments arranged by size: 2174 1952 1078 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7164 7164 Size: 7501 BspHI T'CATG_A Cuts at: 2245 2532 5390 7120 2245 Size: 287 2858 1730 2626 Fragments arranged by size: 2858 2626 1730 287 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3449 4856 844 Size: 2605 1407 3489 Fragments arranged by size: 3489 2605 1407 BsrBI CCG'CTC Cuts at: 1394 1823 5388 1394 Size: 429 3565 3507 Fragments arranged by size: 3565 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2220 5996 6170 65 Size: 1183 972 3776 174 1396 Fragments arranged by size: 3776 1396 1183 972 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6145 6332 1700 Size: 110 4335 187 2869 Fragments arranged by size: 4335 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2575 96 Size: 2479 5022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7501 BssSI C'ACGA_G Cuts at: 5561 6573 5561 Size: 1012 6489 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7501 BstBI TT'CG_AA Cuts at: 6305 6305 Size: 7501 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7501 BstYI r'GATC_y Cuts at: 1972 2251 2517 2524 4098 4358 4831 5198 5583 Size: 279 266 7 1574 260 473 367 385 Cuts at: 5583 5600 7052 7138 7150 7496 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1574 1452 473 385 367 346 279 266 260 86 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6949 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6762 Fragments arranged by size: 6762 683 56 BtsI GCAGTG_nn' Cuts at: 2386 4653 5017 5811 5831 2386 Size: 2267 364 794 20 4056 Fragments arranged by size: 4056 2267 794 364 20 ClaI AT'CG_AT Cuts at: 1554 2901 1554 Size: 1347 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7466 DraI TTT'AAA Cuts at: 3225 4279 4554 4805 3225 Size: 1054 275 251 5921 Fragments arranged by size: 5921 1054 275 251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6508 809 Size: 507 5192 1802 Fragments arranged by size: 5192 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2383 4948 5837 Size: 54 1425 50 275 18 553 2565 889 Cuts at: 5837 7466 8 Size: 1629 43 Fragments arranged by size: 2565 1629 1425 889 553 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4948 1537 Size: 275 3136 4090 Fragments arranged by size: 4090 3136 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3478 3840 3861 3905 4155 5431 1353 Size: 2125 362 21 44 250 1276 3423 Fragments arranged by size: 3423 2125 1276 362 250 44 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6088 6462 6608 387 Size: 1064 138 4499 374 146 1280 Fragments arranged by size: 4499 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2712 3144 4034 5564 6161 6948 Size: 393 524 556 432 890 1530 597 787 Cuts at: 6948 7216 1239 Size: 268 1524 Fragments arranged by size: 1530 1524 890 787 597 556 524 432 393 268 EcoRI G'AATT_C Cuts at: 4810 5188 4810 Size: 378 7123 EcoRV GAT'ATC Cuts at: 2818 2818 Size: 7501 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3479 4580 3479 Size: 1101 6400 FspI TGC'GCA Cuts at: 6007 6007 Size: 7501 HaeII r_GCGC'y Cuts at: 1678 6648 1678 Size: 4970 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2509 2541 3656 3688 4174 Size: 32 135 32 937 32 1115 32 486 Cuts at: 4174 4206 6142 6174 6216 6248 1373 Size: 32 1936 32 42 32 2626 Fragments arranged by size: 2626 1936 1115 937 486 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2798 4153 4338 4845 5091 669 Size: 988 281 860 1355 185 507 246 3079 Fragments arranged by size: 3079 1355 988 860 507 281 246 185 HindIII A'AGCT_T Cuts at: 748 748 Size: 7501 HpaI GTT'AAC Cuts at: 4338 5091 4338 Size: 753 6748 KpnI G_GTAC'C Cuts at: 4826 4826 Size: 7501 MfeI C'AATT_G Cuts at: 2012 5100 2012 Size: 3088 4413 MluI A'CGCG_T Cuts at: 7377 7377 Size: 7501 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2857 6615 6799 1305 Size: 1552 3758 184 2007 Fragments arranged by size: 3758 2007 1552 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2385 10 Size: 54 1425 343 553 5126 Fragments arranged by size: 5126 1425 553 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3192 3323 3429 5459 5818 5977 Size: 648 897 1129 131 106 2030 359 159 Cuts at: 5977 518 Size: 2042 Fragments arranged by size: 2042 2030 1129 897 648 359 159 131 106 MspA1I CmG'CkG Cuts at: 1606 2915 3071 4917 5594 6742 6987 1606 Size: 1309 156 1846 677 1148 245 2120 Fragments arranged by size: 2120 1846 1309 1148 677 245 156 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6949 NdeI CA'TA_TG Cuts at: 387 387 Size: 7501 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7501 NotI GC'GGCC_GC Cuts at: 4948 4948 Size: 7501 NsiI A_TGCA'T Cuts at: 3189 3310 4210 3189 Size: 121 900 6480 Fragments arranged by size: 6480 900 121 NspI r_CATG'y Cuts at: 1132 1229 4859 6404 1132 Size: 97 3630 1545 2229 Fragments arranged by size: 3630 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7501 PciI A'CATG_T Cuts at: 6400 6400 Size: 7501 PflMI CCAn_nnn'nTGG Cuts at: 1108 3981 4538 1108 Size: 2873 557 4071 Fragments arranged by size: 4071 2873 557 PmeI GTTT'AAAC Cuts at: 4805 4805 Size: 7501 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5570 5602 7083 7115 5570 Size: 32 1481 32 5956 Fragments arranged by size: 5956 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7501 PsiI TTA'TAA Cuts at: 3380 5071 3380 Size: 1691 5810 PstI C_TGCA'G Cuts at: 830 3490 4312 4853 830 Size: 2660 822 541 3478 Fragments arranged by size: 3478 2660 822 541 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7501 PvuII CAG'CTG Cuts at: 2915 3071 2915 Size: 156 7345 SalI G'TCGA_C Cuts at: 1936 2796 4843 1936 Size: 860 2047 4594 Fragments arranged by size: 4594 2047 860 SbfI CC_TGCA'GG Cuts at: 4853 4853 Size: 7501 ScaI AGT'ACT Cuts at: 2222 5749 7461 2222 Size: 3527 1712 2262 Fragments arranged by size: 3527 2262 1712 SfcI C'TryA_G Cuts at: 826 1045 2910 3486 4308 4708 4797 4849 5984 Size: 219 1865 576 822 400 89 52 1135 Cuts at: 5984 6665 6856 826 Size: 681 191 1471 Fragments arranged by size: 1865 1471 1135 822 681 576 400 219 191 89 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7501 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7501 SmaI CCC'GGG Cuts at: 1872 4828 1872 Size: 2956 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2560 4289 5605 6506 6768 820 Size: 197 926 617 1729 1316 901 262 1553 Fragments arranged by size: 1729 1553 1316 926 901 617 262 197 SnaBI TAC'GTA Cuts at: 493 5292 493 Size: 4799 2702 SpeI A'CTAG_T Cuts at: 152 2753 3791 152 Size: 2601 1038 3862 Fragments arranged by size: 3862 2601 1038 SphI G_CATG'C Cuts at: 1132 4859 1132 Size: 3727 3774 SspI AAT'ATT Cuts at: 5 52 3538 4084 5425 5 Size: 47 3486 546 1341 2081 Fragments arranged by size: 3486 2081 1341 546 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7501 StyI C'CwwG_G Cuts at: 513 1065 2100 2292 4940 513 Size: 552 1035 192 2648 3074 Fragments arranged by size: 3074 2648 1035 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2375 3413 5528 5545 5698 5883 6328 Size: 1255 795 1038 2115 17 153 185 445 Cuts at: 6328 325 Size: 1498 Fragments arranged by size: 2115 1498 1255 1038 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 2575 2803 Size: 274 80 33 51 1443 243 355 228 Cuts at: 2803 3029 4674 5747 7459 96 Size: 226 1645 1073 1712 138 Fragments arranged by size: 1712 1645 1443 1073 355 274 243 228 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4632 4664 7461 7493 4632 Size: 32 2797 32 4640 Fragments arranged by size: 4640 2797 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7501 XbaI T'CTAG_A Cuts at: 4837 4837 Size: 7501 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3239 3407 4409 3239 Size: 168 1002 6331 Fragments arranged by size: 6331 1002 168 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7501 XmaI C'CCGG_G Cuts at: 1870 4826 1870 Size: 2956 4545 XmnI GAAnn'nnTTC Cuts at: 2676 4047 4454 5630 2676 Size: 1371 407 1176 4547 Fragments arranged by size: 4547 1371 1176 407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5309 276 Size: 53 83 186 936 3775 2468 Fragments arranged by size: 3775 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI