(Circular) (Six-base) MAPSORT of: pFN21AE5570.seq Check: 169 from: 1 to: 6070 pFN21AE5570 6070 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3413 1937 Size: 1476 4594 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 2418 4197 4570 1563 Size: 855 1779 373 3063 Fragments arranged by size: 3063 1779 855 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4133 5517 5785 1632 Size: 2501 1384 268 1917 Fragments arranged by size: 2501 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 2493 4969 5946 1203 Size: 1290 2476 977 1327 Fragments arranged by size: 2476 1327 1290 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 2407 4799 2407 Size: 2392 3678 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 3185 3813 5385 3185 Size: 628 1572 3870 Fragments arranged by size: 3870 1572 628 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2039 3229 3379 3605 3757 5866 1493 Size: 546 1190 150 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 546 226 152 150 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 3395 1870 Size: 73 1452 4545 Fragments arranged by size: 4545 1452 73 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2462 3391 4846 618 Size: 325 217 627 231 444 929 1455 1842 Fragments arranged by size: 1842 1455 929 627 444 325 231 217 BanII G_rGCy'C Cuts at: 1391 3389 1391 Size: 1998 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BfrBI ATG'CAT Cuts at: 2359 3193 2359 Size: 834 5236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2448 6065 2448 Size: 3617 2453 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 4131 5287 1790 Size: 2341 1156 2573 Fragments arranged by size: 2573 2341 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3801 4759 442 Size: 416 53 83 399 24 2384 958 1753 Fragments arranged by size: 2384 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 2804 4730 1239 Size: 1272 293 1926 2579 Fragments arranged by size: 2579 1926 1272 293 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2390 2897 4195 5060 5358 2390 Size: 507 1298 865 298 3102 Fragments arranged by size: 3102 1298 865 507 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2394 4733 882 Size: 617 895 2339 2219 Fragments arranged by size: 2339 2219 895 617 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 2316 6064 2316 Size: 3748 2322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3877 4259 4894 275 Size: 53 83 186 936 2344 382 635 1451 Fragments arranged by size: 2344 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 2840 2870 1178 Size: 30 165 30 1374 30 33 30 4378 Fragments arranged by size: 4378 1374 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2318 3038 3065 3353 3356 1186 Size: 949 183 720 27 288 3 3900 Fragments arranged by size: 3900 949 720 288 183 27 3 BseYI C'CCAG_C Cuts at: 5273 5769 5273 Size: 496 5574 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3084 1163 Size: 573 1348 4149 Fragments arranged by size: 4149 1348 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4131 4216 5287 4131 Size: 85 1071 4914 Fragments arranged by size: 4914 1071 85 BsmI GAATG_Cn' Cuts at: 2361 3193 3581 3674 2361 Size: 832 388 93 4757 Fragments arranged by size: 4757 832 388 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3389 4131 4216 5287 1391 Size: 399 1599 742 85 1071 2174 Fragments arranged by size: 2174 1599 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3425 844 Size: 2581 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2803 4130 5142 2803 Size: 1327 1012 3731 Fragments arranged by size: 3731 1327 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6070 BstXI CCAn_nnnn'nTGG Cuts at: 2210 2210 Size: 6070 BstYI r'GATC_y Cuts at: 1972 2448 3400 3767 4152 4169 5621 5707 5719 Size: 476 952 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 952 476 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2516 3109 513 Size: 552 1451 593 3474 Fragments arranged by size: 3474 1451 593 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 3586 4380 4400 3586 Size: 794 20 5256 Fragments arranged by size: 5256 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 2623 2949 3374 2623 Size: 326 425 5319 Fragments arranged by size: 5319 425 326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2977 5077 809 Size: 507 1661 2100 1802 Fragments arranged by size: 2100 1802 1661 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2753 3517 4406 Size: 54 1425 50 275 18 923 764 889 Cuts at: 4406 6035 8 Size: 1629 43 Fragments arranged by size: 1629 1425 923 889 764 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4000 1353 Size: 2647 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2861 4657 5031 5177 387 Size: 1064 138 1272 1796 374 146 1280 Fragments arranged by size: 1796 1280 1272 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 2804 4133 4730 5517 5785 1239 Size: 393 879 293 1329 597 787 268 1524 Fragments arranged by size: 1524 1329 879 787 597 393 293 268 EcoO109I rG'GnC_Cy Cuts at: 2614 2614 Size: 6070 EcoRI G'AATT_C Cuts at: 3229 3379 3757 3229 Size: 150 378 5542 Fragments arranged by size: 5542 378 150 EcoRV GAT'ATC Cuts at: 2112 3160 2112 Size: 1048 5022 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2393 2425 2777 Size: 32 135 32 508 32 281 32 352 Cuts at: 2777 2809 3061 3093 3149 3181 4711 4743 4785 Size: 32 252 32 56 32 1530 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 1530 508 352 281 252 135 56 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3271 3414 3660 669 Size: 988 281 1333 143 246 3079 Fragments arranged by size: 3079 1333 988 281 246 143 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2485 3006 5184 5368 1305 Size: 1180 521 2178 184 2007 Fragments arranged by size: 2178 2007 1180 521 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4248 Fragments arranged by size: 4248 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4028 4387 4546 518 Size: 648 2862 359 159 2042 Fragments arranged by size: 2862 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3486 4163 5311 5556 1606 Size: 1880 677 1148 245 2120 Fragments arranged by size: 2120 1880 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2516 3109 513 Size: 552 1451 593 3474 Fragments arranged by size: 3474 1451 593 552 NdeI CA'TA_TG Cuts at: 387 2370 387 Size: 1983 4087 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NsiI A_TGCA'T Cuts at: 2361 3195 2361 Size: 834 5236 NspI r_CATG'y Cuts at: 1132 1229 2497 3217 3428 4973 1132 Size: 97 1268 720 211 1545 2229 Fragments arranged by size: 2229 1545 1268 720 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2493 4969 2493 Size: 2476 3594 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6070 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2614 2614 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PstI C_TGCA'G Cuts at: 830 3132 3422 830 Size: 2302 290 3478 Fragments arranged by size: 3478 2302 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 3128 3418 4553 5234 5425 826 Size: 219 2083 290 1135 681 191 1471 Fragments arranged by size: 2083 1471 1135 681 290 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 2876 4174 5075 5337 820 Size: 197 926 462 471 1298 901 262 1553 Fragments arranged by size: 1553 1298 926 901 471 462 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2499 2516 3109 3509 513 Size: 552 1434 17 593 400 3074 Fragments arranged by size: 3074 1434 593 552 400 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4097 4114 4267 4452 4897 325 Size: 1255 2517 17 153 185 445 1498 Fragments arranged by size: 2517 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4316 6028 96 Size: 274 80 33 51 1443 2339 1712 138 Fragments arranged by size: 2339 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2794 2826 2840 2872 6030 6062 2794 Size: 32 14 32 3158 32 2802 Fragments arranged by size: 3158 2802 32 32 32 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3142 3142 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI