(Circular) (Six-base) MAPSORT of: pFN21AE2394.seq Check: 7544 from: 1 to: 7168 pFN21AE2394 7168 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2413 2413 Size: 7168 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2766 3497 4978 278 Size: 53 83 186 936 1230 731 1481 2468 Fragments arranged by size: 2468 1481 1230 936 731 186 83 53 AccI GT'mk_AC Cuts at: 1937 3255 4511 1937 Size: 1318 1256 4594 Fragments arranged by size: 4594 1318 1256 Acc65I G'GTAC_C Cuts at: 4489 4489 Size: 7168 AclI AA'CG_TT Cuts at: 1563 5295 5668 1563 Size: 3732 373 3063 Fragments arranged by size: 3732 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2715 3909 4311 5231 6615 6883 1632 Size: 1083 1194 402 920 1384 268 1917 Fragments arranged by size: 1917 1384 1194 1083 920 402 268 AflII C'TTAA_G Cuts at: 820 1017 4141 820 Size: 197 3124 3847 Fragments arranged by size: 3847 3124 197 AflIII A'CryG_T Cuts at: 1203 3934 6067 7044 1203 Size: 2731 2133 977 1327 Fragments arranged by size: 2731 2133 1327 977 AgeI A'CCGG_T Cuts at: 5999 5999 Size: 7168 AhdI GACnn_n'nnGTC Cuts at: 5897 5897 Size: 7168 AleI CACnn'nnGTG Cuts at: 1166 3912 1166 Size: 2746 4422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7168 AlwNI CAG_nnn'CTG Cuts at: 2050 2089 3299 4911 6483 2050 Size: 39 1210 1612 1572 2735 Fragments arranged by size: 2735 1612 1572 1210 39 ApaI G_GGCC'C Cuts at: 2863 2863 Size: 7168 ApaLI G'TGCA_C Cuts at: 5225 6381 5225 Size: 1156 6012 ApoI r'AATT_y Cuts at: 1493 3386 3565 4477 4703 4855 6964 1493 Size: 1893 179 912 226 152 2109 1697 Fragments arranged by size: 2109 1893 1697 912 226 179 152 AseI AT'TA_AT Cuts at: 160 1032 5722 160 Size: 872 4690 1606 Fragments arranged by size: 4690 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 AvaI C'yCGr_G Cuts at: 1870 1943 2652 4493 1870 Size: 73 709 1841 4545 Fragments arranged by size: 4545 1841 709 73 BamHI G'GATC_C Cuts at: 4498 4865 4498 Size: 367 6801 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 4489 5944 618 Size: 325 217 627 246 2456 1455 1842 Fragments arranged by size: 2456 1842 1455 627 325 246 217 BanII G_rGCy'C Cuts at: 1391 2382 2863 2949 4374 4487 1391 Size: 991 481 86 1425 113 4072 Fragments arranged by size: 4072 1425 991 481 113 86 BbeI G_GCGC'C Cuts at: 2037 2037 Size: 7168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3831 4113 928 Size: 971 1932 282 3983 Fragments arranged by size: 3983 1932 971 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5359 5393 1903 Size: 34 3422 34 3678 Fragments arranged by size: 3678 3422 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2578 2672 5060 6270 2578 Size: 94 2388 1210 3476 Fragments arranged by size: 3476 2388 1210 94 BclI T'GATC_A Cuts at: 1548 2496 4354 1548 Size: 948 1858 4362 Fragments arranged by size: 4362 1858 948 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5779 136 Size: 107 122 71 631 605 4107 1525 Fragments arranged by size: 4107 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7163 7163 Size: 7168 BlpI GC'TnA_GC Cuts at: 2643 2774 4585 2643 Size: 131 1811 5226 Fragments arranged by size: 5226 1811 131 Bme1580I G_kGCm'C Cuts at: 1790 2863 5229 6385 1790 Size: 1073 2366 1156 2573 Fragments arranged by size: 2573 2366 1156 1073 BmgBI CAC'GTC Cuts at: 1318 3403 1318 Size: 2085 5083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2435 4899 5857 Size: 416 53 83 399 24 1018 2464 958 Cuts at: 5857 442 Size: 1753 Fragments arranged by size: 2464 1753 1018 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7168 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 4303 2401 Size: 1902 5266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 3363 3846 5828 1239 Size: 833 1291 483 1982 2579 Fragments arranged by size: 2579 1982 1291 833 483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2697 3206 3887 5293 6158 6456 2697 Size: 509 681 1406 865 298 3409 Fragments arranged by size: 3409 1406 865 681 509 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2627 5831 882 Size: 617 1128 3204 2219 Fragments arranged by size: 3204 2219 1128 617 BsaAI yAC'GTr Cuts at: 493 1204 2792 3229 4959 493 Size: 711 1588 437 1730 2702 Fragments arranged by size: 2702 1730 1588 711 437 BsaBI GATnn'nnATC Cuts at: 3036 3544 3996 7162 3036 Size: 508 452 3166 3042 Fragments arranged by size: 3166 3042 508 452 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2763 3494 4975 Size: 53 83 186 936 501 729 731 1481 Cuts at: 4975 5357 5992 275 Size: 382 635 1451 Fragments arranged by size: 1481 1451 936 731 729 635 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3951 5601 5999 6273 6420 6831 3951 Size: 1650 398 274 147 411 4288 Fragments arranged by size: 4288 1650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2999 3029 1178 Size: 30 165 30 1596 30 5317 Fragments arranged by size: 5317 1596 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2261 2584 3248 4252 1186 Size: 937 138 323 664 1004 4102 Fragments arranged by size: 4102 1004 937 664 323 138 BseYI C'CCAG_C Cuts at: 4147 6371 6867 4147 Size: 2224 496 4448 Fragments arranged by size: 4448 2224 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2814 1163 Size: 573 1078 5517 Fragments arranged by size: 5517 1078 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4618 5379 6407 1540 Size: 275 186 2617 761 1028 2301 Fragments arranged by size: 2617 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2382 2791 2949 4374 5229 5314 6385 2382 Size: 409 158 1425 855 85 1071 3165 Fragments arranged by size: 3165 1425 1071 855 409 158 85 BsmI GAATG_Cn' Cuts at: 2342 2844 3670 4679 4772 2342 Size: 502 826 1009 93 4738 Fragments arranged by size: 4738 1009 826 502 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2770 3887 2770 Size: 1117 6051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2791 2863 2949 4374 4487 5229 Size: 399 592 409 72 86 1425 113 742 Cuts at: 5229 5314 6385 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1425 1071 742 592 409 399 113 86 85 72 BspEI T'CCGG_A Cuts at: 6831 6831 Size: 7168 BspHI T'CATG_A Cuts at: 3322 3424 5057 6787 3322 Size: 102 1633 1730 3703 Fragments arranged by size: 3703 1730 1633 102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2413 4523 844 Size: 1517 52 2110 3489 Fragments arranged by size: 3489 2110 1517 52 BsrBI CCG'CTC Cuts at: 1394 1823 2463 5055 1394 Size: 429 640 2592 3507 Fragments arranged by size: 3507 2592 640 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3091 3645 5663 5837 65 Size: 1183 1843 554 2018 174 1396 Fragments arranged by size: 2018 1843 1396 1183 554 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 5812 5999 1700 Size: 110 226 3776 187 2869 Fragments arranged by size: 3776 2869 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7168 BssSI C'ACGA_G Cuts at: 5228 6240 5228 Size: 1012 6156 BstAPI GCAn_nnn'nTGC Cuts at: 794 2428 794 Size: 1634 5534 BstBI TT'CG_AA Cuts at: 5972 5972 Size: 7168 BstEII G'GTnAC_C Cuts at: 1184 3191 1184 Size: 2007 5161 BstXI CCAn_nnnn'nTGG Cuts at: 3818 3818 Size: 7168 BstYI r'GATC_y Cuts at: 1972 2313 4017 4426 4498 4865 5250 5267 6719 Size: 341 1704 409 72 367 385 17 1452 Cuts at: 6719 6805 6817 7163 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1704 1452 409 385 367 346 341 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5677 Fragments arranged by size: 5677 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2139 487 Size: 683 56 913 5516 Fragments arranged by size: 5516 913 683 56 BtsI GCAGTG_nn' Cuts at: 3907 4684 5478 5498 3907 Size: 777 794 20 5577 Fragments arranged by size: 5577 794 777 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3035 3070 529 Size: 35 2471 35 4627 Fragments arranged by size: 4627 2471 35 35 DraI TTT'AAA Cuts at: 4472 4472 Size: 7168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6175 809 Size: 507 4859 1802 Fragments arranged by size: 4859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 4615 5504 Size: 54 1425 50 275 18 240 2545 889 Cuts at: 5504 7133 8 Size: 1629 43 Fragments arranged by size: 2545 1629 1425 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4615 1537 Size: 275 2803 4090 Fragments arranged by size: 4090 2803 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3165 3605 4008 4209 5098 1353 Size: 1812 440 403 201 889 3423 Fragments arranged by size: 3423 1812 889 440 403 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3797 5755 6129 6275 387 Size: 1064 138 2208 1958 374 146 1280 Fragments arranged by size: 2208 1958 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2380 2947 4372 2380 Size: 567 1425 5176 Fragments arranged by size: 5176 1425 567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2715 3363 3846 3909 4311 5231 Size: 393 440 643 648 483 63 402 920 Cuts at: 5231 5828 6615 6883 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 920 787 648 643 597 483 440 402 393 268 63 EcoNI CCTnn'n_nnAGG Cuts at: 4141 4199 4141 Size: 58 7110 EcoO109I rG'GnC_Cy Cuts at: 2860 2875 3153 2860 Size: 15 278 6875 Fragments arranged by size: 6875 278 15 EcoRI G'AATT_C Cuts at: 4477 4855 4477 Size: 378 6790 EcoRV GAT'ATC Cuts at: 3718 3728 3718 Size: 10 7158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2663 2749 4316 2663 Size: 86 1567 5515 Fragments arranged by size: 5515 1567 86 FspI TGC'GCA Cuts at: 5674 5674 Size: 7168 HaeII r_GCGC'y Cuts at: 1678 2037 6315 1678 Size: 359 4278 2531 Fragments arranged by size: 4278 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2646 2678 3302 3334 3582 Size: 32 135 32 1074 32 624 32 248 Cuts at: 3582 3614 3717 3749 4208 4240 5809 5841 5883 Size: 32 103 32 459 32 1569 32 42 Cuts at: 5883 5915 1373 Size: 32 2626 Fragments arranged by size: 2626 1569 1074 624 459 248 135 103 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4252 4444 4512 4758 669 Size: 988 281 2314 192 68 246 3079 Fragments arranged by size: 3079 2314 988 281 246 192 68 HindIII A'AGCT_T Cuts at: 748 2771 3945 748 Size: 2023 1174 3971 Fragments arranged by size: 3971 2023 1174 HpaI GTT'AAC Cuts at: 4252 4444 4758 4252 Size: 192 314 6662 Fragments arranged by size: 6662 314 192 KasI G'GCGC_C Cuts at: 2033 2033 Size: 7168 KpnI G_GTAC'C Cuts at: 4493 4493 Size: 7168 MfeI C'AATT_G Cuts at: 3690 4767 3690 Size: 1077 6091 MluI A'CGCG_T Cuts at: 7044 7044 Size: 7168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3949 6282 6466 1305 Size: 2644 2333 184 2007 Fragments arranged by size: 2644 2333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5346 Fragments arranged by size: 5346 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3084 3912 3977 5126 5485 5644 518 Size: 648 1918 828 65 1149 359 159 2042 Fragments arranged by size: 2042 1918 1149 828 648 359 159 65 MspA1I CmG'CkG Cuts at: 1606 2024 2969 3299 4584 5261 6409 6654 1606 Size: 418 945 330 1285 677 1148 245 2120 Fragments arranged by size: 2120 1285 1148 945 677 418 330 245 NaeI GCC'GGC Cuts at: 2038 2038 Size: 7168 NarI GG'CG_CC Cuts at: 2034 2034 Size: 7168 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5677 Fragments arranged by size: 5677 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7168 NgoMIV G'CCGG_C Cuts at: 2036 2036 Size: 7168 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7168 NotI GC'GGCC_GC Cuts at: 4615 4615 Size: 7168 NspI r_CATG'y Cuts at: 1132 1229 3938 4526 6071 1132 Size: 97 2709 588 1545 2229 Fragments arranged by size: 2709 2229 1545 588 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7168 PciI A'CATG_T Cuts at: 3934 6067 3934 Size: 2133 5035 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2933 3524 1108 Size: 942 883 591 4752 Fragments arranged by size: 4752 942 883 591 PfoI T'CCnGG_A Cuts at: 2094 2094 Size: 7168 PmeI GTTT'AAAC Cuts at: 4472 4472 Size: 7168 PmlI CAC'GTG Cuts at: 2792 3229 2792 Size: 437 6731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3805 3837 5237 5269 6750 6782 3805 Size: 32 1400 32 1481 32 4191 Fragments arranged by size: 4191 1481 1400 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2875 3153 2875 Size: 278 6890 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7168 PsiI TTA'TAA Cuts at: 4304 4738 4304 Size: 434 6734 PspOMI G'GGCC_C Cuts at: 2859 2859 Size: 7168 PspXI vC'TCGA_Gb Cuts at: 2652 2652 Size: 7168 PstI C_TGCA'G Cuts at: 830 4520 830 Size: 3690 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7168 PvuII CAG'CTG Cuts at: 2969 3299 2969 Size: 330 6838 SacI G_AGCT'C Cuts at: 2382 2949 4374 2382 Size: 567 1425 5176 Fragments arranged by size: 5176 1425 567 SalI G'TCGA_C Cuts at: 1936 4510 1936 Size: 2574 4594 SbfI CC_TGCA'GG Cuts at: 4520 4520 Size: 7168 ScaI AGT'ACT Cuts at: 4163 5416 7128 4163 Size: 1253 1712 4203 Fragments arranged by size: 4203 1712 1253 SfcI C'TryA_G Cuts at: 826 1045 2254 4516 5651 6332 6523 826 Size: 219 1209 2262 1135 681 191 1471 Fragments arranged by size: 2262 1471 1209 1135 681 219 191 SfoI GGC'GCC Cuts at: 2035 2035 Size: 7168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7168 SmaI CCC'GGG Cuts at: 1872 4495 1872 Size: 2623 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2652 2712 3221 3866 4141 5272 Size: 197 926 709 60 509 645 275 1131 Cuts at: 5272 6173 6435 820 Size: 901 262 1553 Fragments arranged by size: 1553 1131 926 901 709 645 509 275 262 197 60 SnaBI TAC'GTA Cuts at: 493 4959 493 Size: 4466 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7168 SphI G_CATG'C Cuts at: 1132 4526 1132 Size: 3394 3774 SspI AAT'ATT Cuts at: 5 52 5092 5 Size: 47 5040 2081 Fragments arranged by size: 5040 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7168 StyI C'CwwG_G Cuts at: 513 1065 2004 2325 2863 2927 4607 513 Size: 552 939 321 538 64 1680 3074 Fragments arranged by size: 3074 1680 939 552 538 321 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2454 2463 4391 5195 5212 5365 5550 Size: 1255 874 9 1928 804 17 153 185 Cuts at: 5550 5995 325 Size: 445 1498 Fragments arranged by size: 1928 1498 1255 874 804 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3200 4161 5414 Size: 274 80 33 51 1443 1223 961 1253 Cuts at: 5414 7126 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1253 1223 961 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7128 7160 7128 Size: 32 7136 Tth111I GACn'n_nGTC Cuts at: 1316 3835 1316 Size: 2519 4649 XbaI T'CTAG_A Cuts at: 4000 4504 4000 Size: 504 6664 XhoI C'TCGA_G Cuts at: 1943 2652 1943 Size: 709 6459 XmaI C'CCGG_G Cuts at: 1870 4493 1870 Size: 2623 4545 XmnI GAAnn'nnTTC Cuts at: 2090 2163 2291 2844 5297 2090 Size: 73 128 553 2453 3961 Fragments arranged by size: 3961 2453 553 128 73 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2764 3495 4976 276 Size: 53 83 186 936 1230 731 1481 2468 Fragments arranged by size: 2468 1481 1230 936 731 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI