Restriction map for
pFN21AE2394
(Linear) (Six-base) MAPSORT of: KIEE2394.seq Check: 4074 from: 1 to: 2463
KIEE2394.orf
With 182 enzymes: SgfI *
January 26, 2012 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 408 2463
Size: 408 2055
AatII G_ACGT'C
Cuts at: 0 761 1492 2463
Size: 761 731 971
Fragments arranged by size:
971 761 731
AccI GT'mk_AC
Cuts at: 0 1250 2463
Size: 1250 1213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1904 2306 2463
Size: 710 1194 402 157
Fragments arranged by size:
1194 710 402 157
AflII C'TTAA_G
Cuts at: 0 2136 2463
Size: 2136 327
AflIII A'CryG_T
Cuts at: 0 1929 2463
Size: 1929 534
AleI CACnn'nnGTG
Cuts at: 0 1907 2463
Size: 1907 556
AlwNI CAG_nnn'CTG
Cuts at: 0 45 84 1294 2463
Size: 45 39 1210 1169
Fragments arranged by size:
1210 1169 45 39
ApaI G_GGCC'C
Cuts at: 0 858 2463
Size: 858 1605
ApoI r'AATT_y
Cuts at: 0 1381 1560 2463
Size: 1381 179 903
Fragments arranged by size:
1381 903 179
AvaI C'yCGr_G
Cuts at: 0 647 2463
Size: 647 1816
BanI G'GyrC_C
Cuts at: 0 28 2463
Size: 28 2435
BanII G_rGCy'C
Cuts at: 0 377 858 944 2369 2463
Size: 377 481 86 1425 94
Fragments arranged by size:
1425 481 377 94 86
BbeI G_GCGC'C
Cuts at: 0 32 2463
Size: 32 2431
BbsI GAAGACnn'nnnn_
Cuts at: 0 1826 2108 2463
Size: 1826 282 355
Fragments arranged by size:
1826 355 282
BciVI GTATCCnnnnn_n'
Cuts at: 0 573 667 2463
Size: 573 94 1796
Fragments arranged by size:
1796 573 94
BclI T'GATC_A
Cuts at: 0 491 2349 2463
Size: 491 1858 114
Fragments arranged by size:
1858 491 114
BlpI GC'TnA_GC
Cuts at: 0 638 769 2463
Size: 638 131 1694
Fragments arranged by size:
1694 638 131
Bme1580I G_kGCm'C
Cuts at: 0 858 2463
Size: 858 1605
BmgBI CAC'GTC
Cuts at: 0 1398 2463
Size: 1398 1065
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 2463
Size: 430 2033
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 396 2298 2463
Size: 396 1902 165
Fragments arranged by size:
1902 396 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1358 1841 2463
Size: 67 1291 483 622
Fragments arranged by size:
1291 622 483 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1201 1882 2463
Size: 692 509 681 581
Fragments arranged by size:
692 681 581 509
BsaI GGTCTCn'nnnn_
Cuts at: 0 622 2463
Size: 622 1841
BsaAI yAC'GTr
Cuts at: 0 787 1224 2463
Size: 787 437 1239
Fragments arranged by size:
1239 787 437
BsaBI GATnn'nnATC
Cuts at: 0 1031 1539 1991 2463
Size: 1031 508 452 472
Fragments arranged by size:
1031 508 472 452
BsaHI Gr'CG_yC
Cuts at: 0 29 758 1489 2463
Size: 29 729 731 974
Fragments arranged by size:
974 731 729 29
BsaWI w'CCGG_w
Cuts at: 0 1946 2463
Size: 1946 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 994 1024 2463
Size: 994 30 1439
Fragments arranged by size:
1439 994 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 256 579 1243 2247 2463
Size: 118 138 323 664 1004 216
Fragments arranged by size:
1004 664 323 216 138 118
BseYI C'CCAG_C
Cuts at: 0 2142 2463
Size: 2142 321
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 2463
Size: 809 1654
BsiHKAI G_wGCw'C
Cuts at: 0 377 786 944 2369 2463
Size: 377 409 158 1425 94
Fragments arranged by size:
1425 409 377 158 94
BsmI GAATG_Cn'
Cuts at: 0 337 839 1665 2463
Size: 337 502 826 798
Fragments arranged by size:
826 798 502 337
BsmBI CGTCTCn'nnnn_
Cuts at: 0 765 1882 2463
Size: 765 1117 581
Fragments arranged by size:
1117 765 581
Bsp1286I G_dGCh'C
Cuts at: 0 377 786 858 944 2369 2463
Size: 377 409 72 86 1425 94
Fragments arranged by size:
1425 409 377 94 86 72
BspHI T'CATG_A
Cuts at: 0 1317 1419 2463
Size: 1317 102 1044
Fragments arranged by size:
1317 1044 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 408 2463
Size: 356 52 2055
Fragments arranged by size:
2055 356 52
BsrBI CCG'CTC
Cuts at: 0 458 2463
Size: 458 2005
BsrDI GCAATG_nn'
Cuts at: 0 1086 1640 2463
Size: 1086 554 823
Fragments arranged by size:
1086 823 554
BsrFI r'CCGG_y
Cuts at: 0 31 2463
Size: 31 2432
BstAPI GCAn_nnn'nTGC
Cuts at: 0 423 2463
Size: 423 2040
BstEII G'GTnAC_C
Cuts at: 0 1186 2463
Size: 1186 1277
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1813 2463
Size: 1813 650
BstYI r'GATC_y
Cuts at: 0 308 2012 2421 2463
Size: 308 1704 409 42
Fragments arranged by size:
1704 409 308 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 134 2463
Size: 134 2329
BtsI GCAGTG_nn'
Cuts at: 0 1902 2463
Size: 1902 561
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1030 1065 2463
Size: 1030 35 1398
Fragments arranged by size:
1398 1030 35
EaeI y'GGCC_r
Cuts at: 0 65 2463
Size: 65 2398
EarI CTCTTCn'nnn_
Cuts at: 0 1160 1600 2003 2204 2463
Size: 1160 440 403 201 259
Fragments arranged by size:
1160 440 403 259 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1792 2463
Size: 1792 671
EcoICRI GAG'CTC
Cuts at: 0 375 942 2367 2463
Size: 375 567 1425 96
Fragments arranged by size:
1425 567 375 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 710 1358 1841 1904 2306 2463
Size: 67 643 648 483 63 402 157
Fragments arranged by size:
648 643 483 402 157 67 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2136 2194 2463
Size: 2136 58 269
Fragments arranged by size:
2136 269 58
EcoO109I rG'GnC_Cy
Cuts at: 0 855 870 1148 2463
Size: 855 15 278 1315
Fragments arranged by size:
1315 855 278 15
EcoRV GAT'ATC
Cuts at: 0 1713 1723 2463
Size: 1713 10 740
Fragments arranged by size:
1713 740 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 658 744 2311 2463
Size: 658 86 1567 152
Fragments arranged by size:
1567 658 152 86
HaeII r_GCGC'y
Cuts at: 0 32 2463
Size: 32 2431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 641 673 1297 1329 1577 1609 1712 1744
Size: 641 32 624 32 248 32 103 32
Cuts at: 1744 2203 2235 2463
Size: 459 32 228
Fragments arranged by size:
641 624 459 248 228 103 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2247 2439 2463
Size: 2247 192 24
Fragments arranged by size:
2247 192 24
HindIII A'AGCT_T
Cuts at: 0 766 1940 2463
Size: 766 1174 523
Fragments arranged by size:
1174 766 523
HpaI GTT'AAC
Cuts at: 0 2247 2439 2463
Size: 2247 192 24
Fragments arranged by size:
2247 192 24
KasI G'GCGC_C
Cuts at: 0 28 2463
Size: 28 2435
MfeI C'AATT_G
Cuts at: 0 1685 2463
Size: 1685 778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1944 2463
Size: 1944 519
MslI CAynn'nnrTG
Cuts at: 0 1079 1907 1972 2463
Size: 1079 828 65 491
Fragments arranged by size:
1079 828 491 65
MspA1I CmG'CkG
Cuts at: 0 19 964 1294 2463
Size: 19 945 330 1169
Fragments arranged by size:
1169 945 330 19
NaeI GCC'GGC
Cuts at: 0 33 2463
Size: 33 2430
NarI GG'CG_CC
Cuts at: 0 29 2463
Size: 29 2434
NgoMIV G'CCGG_C
Cuts at: 0 31 2463
Size: 31 2432
NspI r_CATG'y
Cuts at: 0 1933 2463
Size: 1933 530
PciI A'CATG_T
Cuts at: 0 1929 2463
Size: 1929 534
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 928 1519 2463
Size: 45 883 591 944
Fragments arranged by size:
944 883 591 45
PfoI T'CCnGG_A
Cuts at: 0 89 2463
Size: 89 2374
PmlI CAC'GTG
Cuts at: 0 787 1224 2463
Size: 787 437 1239
Fragments arranged by size:
1239 787 437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1800 1832 2463
Size: 1800 32 631
Fragments arranged by size:
1800 631 32
PpuMI rG'GwC_Cy
Cuts at: 0 870 1148 2463
Size: 870 278 1315
Fragments arranged by size:
1315 870 278
PsiI TTA'TAA
Cuts at: 0 2299 2463
Size: 2299 164
PspOMI G'GGCC_C
Cuts at: 0 854 2463
Size: 854 1609
PspXI vC'TCGA_Gb
Cuts at: 0 647 2463
Size: 647 1816
PvuII CAG'CTG
Cuts at: 0 964 1294 2463
Size: 964 330 1169
Fragments arranged by size:
1169 964 330
SacI G_AGCT'C
Cuts at: 0 377 944 2369 2463
Size: 377 567 1425 94
Fragments arranged by size:
1425 567 377 94
ScaI AGT'ACT
Cuts at: 0 2158 2463
Size: 2158 305
SfcI C'TryA_G
Cuts at: 0 249 2463
Size: 249 2214
SfoI GGC'GCC
Cuts at: 0 30 2463
Size: 30 2433
SmlI C'TyrA_G
Cuts at: 0 647 707 1216 1861 2136 2463
Size: 647 60 509 645 275 327
Fragments arranged by size:
647 645 509 327 275 60
StyI C'CwwG_G
Cuts at: 0 320 858 922 2463
Size: 320 538 64 1541
Fragments arranged by size:
1541 538 320 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 449 458 2386 2463
Size: 449 9 1928 77
Fragments arranged by size:
1928 449 77 9
TatI w'GTAC_w
Cuts at: 0 1195 2156 2463
Size: 1195 961 307
Fragments arranged by size:
1195 961 307
Tth111I GACn'n_nGTC
Cuts at: 0 1830 2463
Size: 1830 633
XbaI T'CTAG_A
Cuts at: 0 1995 2463
Size: 1995 468
XhoI C'TCGA_G
Cuts at: 0 647 2463
Size: 647 1816
XmnI GAAnn'nnTTC
Cuts at: 0 85 158 286 839 2463
Size: 85 73 128 553 1624
Fragments arranged by size:
1624 553 128 85 73
ZraI GAC'GTC
Cuts at: 0 759 1490 2463
Size: 759 731 973
Fragments arranged by size:
973 759 731
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BciVI
BclI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgZI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MmeI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI
PspXI PvuII SacI ScaI SfcI SfoI SmlI StyI
TaqII TatI Tth111I XbaI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BfrBI BglI BglII BmtI Bpu10I BsiEI BsiWI BspEI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgI ClaI
DraI DraIII DrdI EagI EcoRI FseI FspI FspAI
KpnI MluI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PshAI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XcmI XmaI