################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 468 GACGTC GACGT^C AcyI 468 GACGTC G[GA]^CG[CT]C AfaI 459 GTAC GT^AC AlwNI 305 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 27 GAGCTC G[AT]GC[AT]^C AvaI 482 CCCGGG C^[CT]CG[GA]G BanI 411 GGTGCC G^G[CT][GA]CC BanII 27 GAGCTC G[GA]GC[CT]^C BclI 50 TGATCA T^GATCA BfaI 394 CTAG C^TAG BmyI 27 GAGCTC G[AGT]GC[ACT]^C BsaAI 460 TACGTG [CT]AC^GT[GA] BsaHI 468 GACGTC G[GA]^CG[CT]C BsgI 454 GTGCAG GTGCAG(16/14) BsiHKAI 27 GAGCTC G[AT]GC[AT]^C BsmAI 466 GAGAC GTCTC(1/5) BsmBI 466 GAGACG CGTCTC(1/5) BsoBI 482 CCCGGG C^[CT]CG[GA]G Bsp1286I 27 GAGCTC G[AGT]GC[ACT]^C BspMI 408 GCAGGT ACCTGC(4/8) BsrBI 400 GAGCGG CCGCTC(-3/-3) BstNI 428 CCAGG CC^[AT]GG Cac8I 452 GCGTGC GC[ACGT]^[ACGT]GC DpnII 51 GATC ^GATC DraI 595 TTTAAA TTT^AAA DraIII 43 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 442 GAAGAG CTCTTC(1/4) EcoNI 39 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 316 GAATTC G^AATTC EcoRII 428 CCAGG ^CC[AT]GG EcoT14I 32 CCTTGG C^C[AT][AT]GG FbaI 50 TGATCA T^GATCA HapII 483 CCGG C^CGG Hin1I 468 GACGTC G[GA]^CG[CT]C HpaII 483 CCGG C^CGG HphI 48 GGTGA GGTGA(8/7) Ksp632I 442 GAAGAG CTCTTC(1/4) MaeI 394 CTAG C^TAG MboI 51 GATC ^GATC MseI 596 TTAA T^TAA MspI 483 CCGG C^CGG MvaI 428 CCAGG CC^[AT]GG NdeII 51 GATC ^GATC NlaIV 411 GGTGCC GG[ACGT]^[ACGT]CC RsaI 459 GTAC GT^AC SacI 27 GAGCTC GAGCT^C Sau3AI 51 GATC ^GATC SmaI 482 CCCGGG CCC^GGG StyI 32 CCTTGG C^C[AT][AT]GG TaqI 234 TCGA T^CGA Tru9I 596 TTAA T^TAA TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 234 TCGA T^CGA XmaCI 482 CCCGGG C^CCGGG XmaI 482 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 316 GAATTC [GA]^AATT[CT] 359 AAATTC AluI 28 AGCT AG^CT 141 AGCT ApoI 316 GAATTC [GA]^AATT[CT] 359 AAATTC BbsI 121 GAAGAC GAAGAC(2/6) 477 GAAGAC BcnI 482 CCCGG CC^[GC]GG 483 CCGGG BpuAI 121 GAAGAC GAAGAC(2/6) 477 GAAGAC BsaJI 32 CCTTGG C^C[ACGT][ACGT]GG 482 CCCGGG BseRI 397 GAGGAG GAGGAG(10/8) 421 GAGGAG HinfI 295 GAATC G^A[ACGT]TC 330 GATTC MspA1I 63 CAGCGG C[AC]G^C[GT]G 206 CCGCTG MwoI 62 GCAGCGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 566 GCAAGAAAAGC NciI 482 CCCGG CC^[GC]GG 483 CCGGG TfiI 295 GAATC G^A[AT]TC 330 GATTC Tsp509I 317 AATT ^AATT 360 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI Asp700I BbvI BsiYI BslI BssKI Cfr13I Fnu4HI HaeIII ItaI MaeII MboII MnlI NlaIII Sau96I ScrFI TseI XmnI