################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BalI BbeI BbvCI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 251 GGTACC G^GTACC AccI 377 GTAGAC GT^[AC][GT]AC AcsI 121 GAATTT [GA]^AATT[CT] ApoI 121 GAATTT [GA]^AATT[CT] Asp718I 251 GGTACC G^GTACC BamHI 762 GGATCC G^GATCC BbrPI 1701 CACGTG CAC^GTG BbsI 1393 GAAGAC GAAGAC(2/6) BcgI 608 GCAACCGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1509 TGATCA T^GATCA BglI 1117 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1373 CTCCAG CTGGAG(16/14) BpuAI 1393 GAAGAC GAAGAC(2/6) BsaAI 1701 CACGTG [CT]AC^GT[GA] BsaWI 1175 ACCGGA [AT]^CCGG[AT] Bsp1407I 1082 TGTACA T^GTACA BspMI 209 ACCTGC ACCTGC(4/8) BsrGI 1082 TGTACA T^GTACA BssSI 1576 CTCGTG CACGAG(-5/-1) BstBI 264 TTCGAA TT^CGAA BstEII 564 GGTAACC G^GT[ACGT]ACC BstPI 564 GGTAACC G^GT[ACGT]ACC Cac8I 1122 GCTGGC GC[ACGT]^[ACGT]GC CfoI 1665 GCGC GCG^C DraI 933 TTTAAA TTT^AAA DraII 1105 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 1115 TGGCCG [CT]^GGCC[GA] EcoO109I 1105 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 564 GGTAACC G^GT[ACGT]ACC EcoT14I 1502 CCTTGG C^C[AT][AT]GG EcoT22I 455 ATGCAT ATGCA^T FbaI 1509 TGATCA T^GATCA HapII 1176 CCGG C^CGG HgaI 1200 GACGC GACGC(5/10) HhaI 1665 GCGC GCG^C HinP1I 1665 GCGC G^CGC HpaII 1176 CCGG C^CGG KpnI 251 GGTACC GGTAC^C MaeII 1702 ACGT A^CGT MspI 1176 CCGG C^CGG NdeI 653 CATATG CA^TATG NsiI 455 ATGCAT ATGCA^T NspI 1673 ACATGC [GA]CATG^[CT] NspV 264 TTCGAA TT^CGAA PacI 1531 TTAATTAA TTAAT^TAA PmaCI 1701 CACGTG CAC^GTG PmlI 1701 CACGTG CAC^GTG PpuMI 1105 GGGACCC [GA]G^G[AT]CC[CT] PvuII 534 CAGCTG CAG^CTG SexAI 568 ACCTGGT A^CC[AT]GGT SfcI 1143 CTACAG C^T[GA][CT]AG SfuI 264 TTCGAA TT^CGAA SmlI 1440 CTTGAG C^T[CT][GA]AG SspBI 1082 TGTACA T^GTACA StyI 1502 CCTTGG C^C[AT][AT]GG Tsp45I 976 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 902 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 1146 CAGGCACTG AocI 907 CCTGAGG CC^T[ACGT]AGG 1786 CCTAAGG BanI 251 GGTACC G^G[CT][GA]CC 1096 GGCACC BciVI 678 GGATAC GTATCC(6/5) 1234 GTATCC BsmFI 1105 GGGAC GGGAC(10/14) 199 GTCCC BstYI 273 AGATCC [GA]^GATC[CT] 762 GGATCC Bsu36I 907 CCTGAGG CC^T[ACGT]AGG 1786 CCTAAGG EarI 115 GAAGAG CTCTTC(1/4) 1424 GAAGAG Eco81I 907 CCTGAGG CC^T[ACGT]AGG 1786 CCTAAGG Ksp632I 115 GAAGAG CTCTTC(1/4) 1424 GAAGAG MflI 273 AGATCC [GA]^GATC[CT] 762 GGATCC MslI 653 CATATGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1674 CATGCCTGTG MspA1I 534 CAGCTG C[AC]G^C[GT]G 560 CAGCGG MwoI 1117 GCCGTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1126 GCGGCGAGTGC NlaIII 219 CATG CATG^ 1674 CATG SacI 1251 GAGCTC GAGCT^C 1776 GAGCTC SspI 61 AATATT AAT^ATT 1431 AATATT XhoII 273 AGATCC [GA]^GATC[CT] 762 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI